SAMN18806508
External Id
Lomonosov Moscow State University, Moscow, Russia
INSDC center name
2022-05-19T00:50:14.563Z
INSDC first public
2022-05-19T00:50:14.563Z
INSDC last update
SRS8736484
INSDC secondary accession
live
INSDC status
starch (5 g/kg) and K2HPO4 + (NH4)2SO4 (1 g/kg)
Introduced_nutrificants
Metagenome or environmental
NCBI submission model
Metagenome.environmental.1.0
NCBI submission package
28 days
Period
SRS8736484
SRA accession
non-treated (control for pesticides mix)
Treatment
2019
collection_date
Agrosoddy-podzolic soil was dried at 40 ℃ and sieved through a 1 mm sieve. 100 g of soil was mixed in a mortar with 20 ml of water (control variants) or with 20 ml of water supplemented with pesticide preparations or their mixture (application rate =). After that, the soil samples were placed in 250 ml glass jars. Agrosoddy-podzolic soil is rather poor (Corg=1.5%), therefore microbiological processes go on in it rather slowly, which can complicate monitoring the state of the microbial community. Because of this, we decided to include in some experiment variants with the initiation of succession due to the addition of an additional carbon source. Preliminary experiments with measuring carbon dioxide emissions showed that starch in an amount of 5 g/kg of air-dry soil was best suited for this purpose. Along with the starch, we also introduced mineral salts into the soil in order to avoid NPK limitation. Moreover, we introduced K2HPO4 and (NH4)2SO4 in an amount of 1 g/kg.
description
Russia: Moscow
geo_loc_name
Agrosoddy-podzolic soil (horizons O, A)
isolation_source
55.712314 N 37.543667 E
lat_lon
soil metagenome
http://purl.obolibrary.org/obo/NCBITaxon_410658
organism
Agrosoddy-podzolic soil with introduced starch (5 g/kg) and K2HPO4 + (NH4)2SO4 (1 g/kg), non-treated (control for pesticides mix), 28 days
title