SAMN18747484
External Id
The First Affiliated Hospital of Sun Yat-sen University
INSDC center name
2022-03-24T00:00:00Z
INSDC first public
2022-03-24T02:27:21.973Z
INSDC last update
SRS8707611
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
SRS8707611
SRA accession
HNSCC cells
cell type
normal
http://purl.obolibrary.org/obo/PATO_0000461
genotype
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
organism
HNSCC
source_name
WT_ribo
title
To immobilize initiating ribosomes, harringtonine was added to cell culture medium to a final concentration of 2 µg/ml. Cells were Incubated for 120 s with harringtonine in the incubator. Next, to block translational elongation, cycloheximide was added to cell culture medium to a final concentration of 100 µg/ml. Cells were mixed well and proceeded immediately to cell lysis.
treatment