SAMN18613429
External Id
Nanjing Medical University
INSDC center name
2022-04-13T18:40:42.793Z
INSDC first public
2022-04-13T18:40:42.793Z
INSDC last update
SRS8633852
INSDC secondary accession
live
INSDC status
Human
NCBI submission model
Human.1.0
NCBI submission package
SRS8633852
SRA accession
60 days in vitro
age
WiCell
biomaterial_provider
H9
cell_line
To obtain a single-cell suspension, 5-7 organoids at D60 were randomly selected into a 1.5 ml EP tube with 1 ml of TrypLE (Life Technologies) and incubated in a 37 degrees Celsius incubator for 40 min with gentle agitation every 5-8 min. The organoids were then washed with DPBS (Life Technologies) 3 times and gently dissociated into single cells with a 200 microliter pipette.
isolate
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
organism
female
sex
human brain organoids
tissue
Human sample from Homo sapiens
title