characteristics.text: SAMN18237358
characteristics.characteristic: External Id
characteristics.text: The Chinese University of Hong Kong
characteristics.characteristic: INSDC center name
characteristics.text: 2023-01-01T00:51:58.373Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-01-01T00:51:58.373Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS8432823
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Model organism or animal
characteristics.characteristic: NCBI submission model
characteristics.text: Model.organism.animal.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS8432823
characteristics.characteristic: SRA accession
characteristics.text: 10 weeks
characteristics.characteristic: age
characteristics.text: A bead-based strategy to screen viable CD45- cells was applied. The whole mouse aortae (n=3) of the Western diet group were isolated after intracardiac perfusion with ice-cold PBS. The finely cut aortae were incubated in 1x aortic dissociation enzyme cocktail (450 U/mL collagenase type I, 60 U/mL DNase I, and 60U/mL hyaluronidase) at 37oC for 1 h. The digestion was halted by adding FACS buffer (2 mM EDTA, 2% FBS). The cell suspension was transferred through 50 m cell strainer for subsequent incubation with dead cell removal microbeads (Miltenyi Biotec, North Rhine-Westphalia, Germany) at room temperature for 15 min. Viable cells were collected by transferring cell suspension through a MS column (Miltenyi Biotec) on a magnet. Later, the viable cells were incubated with CD45 microbeads (Miltenyi Biotec) on ice for 15 min for negative selection through a LS column (Miltenyi Biotec) on a magnet. The flow-through cells were centrifuged and resuspended in 0.04% BSA-PBS for droplet-based scRNA-seq.
characteristics.characteristic: description
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Single cell
characteristics.characteristic: sample_type
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: ApoE-/-
characteristics.characteristic: strain
characteristics.text: Aortae
characteristics.characteristic: tissue
characteristics.text: ApoE-/- mice fed with Western diet
characteristics.characteristic: title
characteristics.text: Western diet
characteristics.characteristic: treatment