characteristics.text: SAMN10584743
characteristics.characteristic: External Id
characteristics.text: Helmholtz Zentrum Muenchen
characteristics.characteristic: INSDC center name
characteristics.text: 2020-01-20T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-01-17T21:06:07.233Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS4175972
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Metagenome or environmental
characteristics.characteristic: NCBI submission model
characteristics.text: Metagenome.environmental.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS4175972
characteristics.characteristic: SRA accession
characteristics.text: 2012-09-25
characteristics.characteristic: collection date
characteristics.text: Biological soil crust grown on the bulk soil from the Lieberose sand dune and sampled after ten months of incubation, replicate 2
characteristics.characteristic: description
characteristics.text: Germany: Lieberose
characteristics.characteristic: geo loc name
characteristics.text: biological soil crust
characteristics.characteristic: isolation source
characteristics.text: 51.55 N 14.22 E
characteristics.characteristic: lat lon
characteristics.text: biocrust metagenome
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_1710357
characteristics.characteristic: organism
characteristics.text: DNA was extracted using the ‘Genomic DNA from soil’ NucleoSpin Soil Kit (Macherey-Nagel, DE). DNA was sheared using an E220 Focused-ultrasonicator (Covaris, USA). Library preparation was performed using the NEBNext Ultra DNA Library Prep Kit for Illumina and the NEBNext Multiplex Oligos for Illumina (both New England Biolabs, UK). Sequencing was performed on a MiSeq sequencer using a MiSeq Reagent Kit v3 for 600 cycle (Illumina, USA).
characteristics.characteristic: samp mat process
characteristics.text: L22
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002225
characteristics.characteristic: source_material_id
characteristics.text: Metagenome or environmental sample from biocrust metagenome
characteristics.characteristic: title