characteristics.text: SAMN14918461
characteristics.characteristic: External Id
characteristics.text: University of Bremen
characteristics.characteristic: INSDC center name
characteristics.text: 2020-05-14T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-02-14T21:08:37.487Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS6658885
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: MIMAG
characteristics.characteristic: NCBI submission model
characteristics.text: MIMAG.6.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS6658885
characteristics.characteristic: SRA accession
characteristics.text: This BioSample is a metagenomic assembly obtained from the marine sediment metagenome BioSamples: SAMN14820628, SAMN14820629, and SAMN14820630
characteristics.characteristic: derived-from
characteristics.text: Keywords: GSC:MIxS;MIMAG:6.0
characteristics.characteristic: description
characteristics.text: 1
characteristics.characteristic: environmental sample
characteristics.text: North Sea: German Bight (Helgoland Mud Area)
characteristics.characteristic: geo loc name
characteristics.text: BM5_bin.12
characteristics.characteristic: isolate
characteristics.text: marine sediment
characteristics.characteristic: isolation source
characteristics.text: 54.052300 N 7.580400 E
characteristics.characteristic: lat lon
characteristics.text: marine sediment metagenome
characteristics.characteristic: metagenome source
characteristics.text: 1
characteristics.characteristic: metagenomic
characteristics.text: Mycoplasmatota bacterium
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_2231116
characteristics.characteristic: organism
characteristics.text: obligate anaerobe
characteristics.characteristic: rel_to_oxygen
characteristics.text: Enrichment was prepared using 5 mL volume from 4th transfer as starting material in a final volume of 50 mL widdel medium. Sample name identifies the unique information of each sample including the target 16S rRNA gene and the phase of the enrichment transfer.
characteristics.characteristic: samp mat process
characteristics.text: 5 mL enrichment was centrifuged down to 1 mL volume which was used for DNA extraction
characteristics.characteristic: samp_size
characteristics.text: metagenomic assembly
characteristics.characteristic: sample-type
characteristics.text: Benzoate + magnetite enrichment at 30ºC after the fifth transfer completely metabolised benzoate coupled to magnetite reduction. 5mL was collected from the enrichment bottle and stored at -20ºC prior to DNA extraction and metagenome sequencing. Pooled DNA from three replicates of the BM5 enrichment used for metagenome sequencing. BM5_bin.12 is the reassembled metagenome assembled genome
characteristics.characteristic: source_material_id
characteristics.text: MIMAG Metagenome-assembled Genome sample from Mycoplasmatota bacterium
characteristics.characteristic: title