characteristics.text: SAMN27594069
characteristics.characteristic: External Id
characteristics.text: Shanghai veterinary research institute
characteristics.characteristic: INSDC center name
characteristics.text: 2023-05-01T00:52:13.993Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-05-01T00:52:13.993Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS12623996
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Viral
characteristics.characteristic: NCBI submission model
characteristics.text: Virus.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS12623996
characteristics.characteristic: SRA accession
characteristics.text: not collected
characteristics.characteristic: breed
characteristics.text: 2021-01-01
characteristics.characteristic: collection_date
characteristics.text: not collected
characteristics.characteristic: cultivar
characteristics.text: A recombinant virus was successfully generated by homologous recombination through co-transfection with the transfer vector and then infected with ASFV GZ201801 wild type virus. The deletion mutant was purified by successive rounds of plaque purification and limiting dilution. The purified recombinant virus was named ASFV-GZdeltaI73R, and its genome sequence was analysed by nanopore sequencing, confirming the successful deletion of I73R in the recombinant virus.
characteristics.characteristic: description
characteristics.text: China
characteristics.characteristic: geo_loc_name
characteristics.text: Sus scrofa
characteristics.characteristic: host
characteristics.text: virus suspension
characteristics.characteristic: isolate
characteristics.text: cells
characteristics.characteristic: isolation_source
characteristics.text: African swine fever virus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10497
characteristics.characteristic: organism
characteristics.text: ASFV GZ201801
characteristics.characteristic: strain
characteristics.text: Viral sample from African swine fever virus
characteristics.characteristic: title