characteristics.text: SAMN26135468
characteristics.characteristic: External Id
characteristics.text: UNC Charlotte
characteristics.characteristic: INSDC center name
characteristics.text: 2022-02-21T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-03-09T11:36:45.843Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS12060130
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Plant
characteristics.characteristic: NCBI submission model
characteristics.text: Plant.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS12060130
characteristics.characteristic: SRA accession
characteristics.text: F3H-OX4 (VF36 with p35S:F3H transgene)
characteristics.characteristic: cultivar
characteristics.text: Anthers of flowers from each genotype were harvested into a 15mL falcon tube and resuspended in 5000uL of pollen germination media (PGM). 2000uL was removed and divided into two 1.5mL Eppendorf tubes per genotype and incubated in a 28C water bath for 30 minutes. One of the tubes was moved to a 34C water bath and both tubes were incubated for an additional 90 minutes. The samples were centrifuged, PGM removed, flash frozen in liquid nitrogen, and stored at -80 until extraction with the Qiagen RNeasy Plant Mini Kit.
characteristics.characteristic: design description
characteristics.text: flowering
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0007016
characteristics.characteristic: dev stage
characteristics.text: F3H-OX4-120min-28-34C-R2_R1_001.fastq.gz
characteristics.characteristic: filename
characteristics.text: F3H-OX4-120min-28-34C-R2_R2_001.fastq.gz
characteristics.characteristic: filename2
characteristics.text: fastq
characteristics.characteristic: filetype
characteristics.text: USA: Greensboro, North Carolina
characteristics.characteristic: geo loc name
characteristics.text: Illumina HiSeq 4000
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0008563
characteristics.characteristic: instrument model
characteristics.text: OX4.Stress.2
characteristics.characteristic: library id
characteristics.text: paired
characteristics.characteristic: library layout
characteristics.text: PolyA
characteristics.characteristic: library_selection_(click_for_details)
characteristics.text: TRANSCRIPTOMIC
characteristics.characteristic: library_source_(click_for_details)
characteristics.text: RNA-Seq
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002770
characteristics.characteristic: library_strategy_(click_for_details)
characteristics.text: Solanum lycopersicum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4081
characteristics.characteristic: organism
characteristics.text: ILLUMINA
characteristics.characteristic: platform_(click_for_details)
characteristics.text: pollen tube
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001004
characteristics.characteristic: tissue
characteristics.text: RNA-Seq of Solanum lycopersicum: F3H-OX4 (VF36 with p35S:F3H transgene) pollen tube at 34C
characteristics.characteristic: title
characteristics.text: RNA-Seq of Solanum lycopersicum: F3H-OX4 (VF36 with p35S:F3H transgene) pollen tube at 34C
characteristics.characteristic: title