characteristics.text: SAMN27575416
characteristics.characteristic: External Id
characteristics.text: Shanghai veterinary research institute
characteristics.characteristic: INSDC center name
characteristics.text: 2023-05-01T00:50:33.723Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-05-01T00:50:33.723Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS12604808
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Microbe, viral or environmental
characteristics.characteristic: NCBI submission model
characteristics.text: Microbe.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS12604808
characteristics.characteristic: SRA accession
characteristics.text: 2022-03-01
characteristics.characteristic: collection_date
characteristics.text: To characterize the RNA-binding specificity of pI73R during ASFV infection, a recombinant ASFV-GZmI73R-HA virus was generated with pI73R tagged at the carboxyl end with the human influenza virus Hemagglutinin Tag (HA) based on ASFV Chinese strain GZ201801, and the RNA Immunoprecipitation sequencing (RIP-Seq) assay was performed with BMDM cells infected with ASFV-GZ-mI73RHA virus. The ASFV pI73R-RNA complexes were enriched using HA-Tag antibody, the eluted RNA was analyzed by high-throughput sequencing technology.
characteristics.characteristic: description
characteristics.text: China
characteristics.characteristic: geo_loc_name
characteristics.text: Sus scrofa
characteristics.characteristic: host
characteristics.text: lysated by PIPA buffer
characteristics.characteristic: isolate
characteristics.text: cells
characteristics.characteristic: isolation_source
characteristics.text: African swine fever virus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10497
characteristics.characteristic: organism
characteristics.text: cell culture
characteristics.characteristic: sample_type
characteristics.text: Microbe sample from African swine fever virus
characteristics.characteristic: title