characteristics.text: SAMN25694603
characteristics.characteristic: External Id
characteristics.text: Institute of Molecular Genetics, National Research Centre Kurchatov Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2023-02-05T00:50:18.410Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-02-05T00:50:18.410Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS11894825
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Model organism or animal
characteristics.characteristic: NCBI submission model
characteristics.text: Model.organism.animal.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS11894825
characteristics.characteristic: SRA accession
characteristics.text: two-month-old
characteristics.characteristic: age
characteristics.text: Institute of Molecular Genetics, National Research Centre Kurchatov Institute, 123182, Russia, Moscow; Pirogov Russian National Research Medical University, 117997, Russia, Moscow
characteristics.characteristic: biomaterial_provider
characteristics.text: tMCAO in experimental rats was initiated by endovascular occlusion of right middle cerebral artery using monofilament for 90 minutes followed by reperfusion. The anaesthesia was induced by 3% isoflurane and maintained by 1.5-2% isoflurane. Anesthesia was used only for the filament insertion and removal, while in between these procedures the rats were taken out of anesthesia. At 24 h after tMCAO operation, rats were decapitated. The subcortical structures of the brain were isolated from the right (ipsilateral) hemisphere. mRNA fraction from total RNA of rat subcortex was used for high-throughput RNA-sequencing (RNA-Seq). Sequencing was carried out using an Illumina HiSeq 1500 instrument. At least 10 million reads (1/50 nt) were generated.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: dev_stage
characteristics.text: Rattus norvegicus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10116
characteristics.characteristic: organism
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: Wistar
characteristics.characteristic: strain
characteristics.text: subcortex
characteristics.characteristic: tissue
characteristics.text: Model organism or animal sample from Rattus norvegicus
characteristics.characteristic: title
characteristics.text: rat 122, tMCAO, right (ipsilateral) hemisphere
characteristics.characteristic: treatment