characteristics.text: SAMN27070027
characteristics.characteristic: External Id
characteristics.text: Cancer Science Institute of Singapore
characteristics.characteristic: INSDC center name
characteristics.text: 2023-02-21T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-02-21T23:44:00.917Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS12428243
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS12428243
characteristics.characteristic: SRA accession
characteristics.text: B16/F10
characteristics.characteristic: cell line
characteristics.text: sgRNA
characteristics.characteristic: expression manipulation technique
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: murine melanoma cell line
characteristics.characteristic: source_name
characteristics.text: B16/F10-Cas9
characteristics.characteristic: title
characteristics.text: CRISPR_v2_puro empty vector was transfected into B16/F10 cells via lentivirus. Two weeks (at least) after viral transfection, total RNA was extracted for NGS seq.
characteristics.characteristic: treatment