characteristics.text: SAMN26354320
characteristics.characteristic: External Id
characteristics.text: Zhengzhou University
characteristics.characteristic: INSDC center name
characteristics.text: 2023-05-05T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-05-05T03:52:01.942Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS12194980
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Metagenome or environmental
characteristics.characteristic: NCBI submission model
characteristics.text: Metagenome.environmental.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS12194980
characteristics.characteristic: SRA accession
characteristics.text: 2020-11-17
characteristics.characteristic: collection_date
characteristics.text: Brassica rapa
characteristics.characteristic: host
characteristics.text: BrCHR39-silenced lines
characteristics.characteristic: isolation_source
characteristics.text: not collected
characteristics.characteristic: lat_lon
characteristics.text: plant metagenome
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_1297885
characteristics.characteristic: organism
characteristics.text: The stems and axillary buds of the wild-type and BrCHR39-silenced brassica rapa grown for 10 weeks were collected with three biological replicates.
characteristics.characteristic: samp_collect_device
characteristics.text: The total DNA was extracted using the TRIzol reagent QIAamp Fast DNA Tissue Kit (Qiagen, Dusseldorf, Germany). Then the pair-end 2×150 bp sequencing on an Illumina Hiseq 4002 platform was performed in the LC Sciences and the raw data was to pre-process. The methylation level was identified via a sliding-window approach, which determined by the ratio of the number of reads supporting cytosines (methylated) over total reads (methylated and non-methylated) using perl scripts in house and MethPipe.
characteristics.characteristic: samp_mat_process
characteristics.text: S_stem
characteristics.characteristic: title