characteristics.text: SAMN29883286
characteristics.characteristic: External Id
characteristics.text: INRAE
characteristics.characteristic: INSDC center name
characteristics.text: 2023-01-03T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-01-03T05:14:02.219Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS14056763
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Model organism or animal
characteristics.characteristic: NCBI submission model
characteristics.text: Model.organism.animal.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS14056763
characteristics.characteristic: SRA accession
characteristics.text: Isabelle Darboux (INRAE, UMR 1333 DGIMI, France)
characteristics.characteristic: biomaterial_provider
characteristics.text: S. frugiperda larvae (corn strain) were maintained in the DGIMI laboratory for many years and reared on the Poitout artificiel diet (Poitout S et al, Ann Nutr Aliment 28: 173-187, 1974) at 25°C under a photoperiod of 16h light and 8h dark. Mock-infected larvae were injected with an equivalent volume of Phosphate-buffered alone (PBS). Hemolymph was collected 72h after injection. Hemocytes were harvested by centrigugation at 800 x g for 5 min and washed in PBS. The cell pellet was then resuspended in the TRK lysis buffer of the E.Z.N.A.® total RNA kit I (OMEGA). Total RNA was extracted using the E.Z.N.A.® total RNA kit I according to the manufacturer’s instructions (OMEGA). RNA samples were treated with TURBO DNA-free kit (Ambion) to eliminate potential genomic DNA contamination. RNA concentration and integrity were assessed using a NanoDrop ND-1000 spectrophotometer and an Agilent 2100 Bioanalyzer, respectively. Total RNA with RNA integrity (RIN) scores >= 9 were retained for RNA sequencing. For each replicate, total RNA was extracted from 25-28 control larvae.
characteristics.characteristic: description
characteristics.text: larvae stage 5
characteristics.characteristic: dev_stage
characteristics.text: PBS
characteristics.characteristic: infection
characteristics.text: Spodoptera frugiperda
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7108
characteristics.characteristic: organism
characteristics.text: biological replicate 1
characteristics.characteristic: replicate
characteristics.text: tissue sample
characteristics.characteristic: sample_type
characteristics.text: corn
characteristics.characteristic: strain
characteristics.text: hemocyte
characteristics.characteristic: tissue
characteristics.text: Spodoptera frugiperda hemocyte
characteristics.characteristic: title