characteristics.text: SAMN29883282
characteristics.characteristic: External Id
characteristics.text: INRAE
characteristics.characteristic: INSDC center name
characteristics.text: 2023-01-03T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-01-03T05:14:01.959Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS14056759
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Model organism or animal
characteristics.characteristic: NCBI submission model
characteristics.text: Model.organism.animal.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS14056759
characteristics.characteristic: SRA accession
characteristics.text: Isabelle Darboux (INRAE, UMR 1333 DGIMI, France)
characteristics.characteristic: biomaterial_provider
characteristics.text: S. frugiperda larvae (corn strain) were maintained in the DGIMI laboratory for many years and reared on the Poitout artificiel diet (Poitout S et al, Ann Nutr Aliment 28: 173-187, 1974) at 25°C under a photoperiod of 16h light and 8h dark. The JcDV stock was prepared as reported in Mutuel D et al (Virology 403: 137-144, 2010). In brief, S. frugiperda larvae were infected with JcDV by oral ingestion. After 7-10 days, JcDV was extracted from dead larvae, purified on salt density gradient, and then dialysed. JcDV concentration was estimated by quantitative PCR and expressed as « viral genome/ml » (vg/ml). The virus stock was maintained at -20°C until use. JcDV was injected into fifth-instar S. frugiperda larvae (1-day-old). A viral suspension of 7.2e10 vg of JcDV was injected in each larva. Mortality in JcDV-infected larvae was observed from 72h after infection. At this time, the mortality ranged between 20-36% in the three replicates. Only JcDV-infected larvae with low signs of pathogenesis were used for the preparation of RNAs in order to avoid RNA degradation. After collection of hemolymph, hemocytes were harvested by centrigugation at 800 x g for 5 min and washed in PBS. The cell pellet was then resuspended in the TRK lysis buffer of the E.Z.N.A.® total RNA kit I (OMEGA). Total RNA was extracted using the E.Z.N.A.® total RNA kit I according to the manufacturer’s instructions (OMEGA). RNA samples were treated with TURBO DNA-free kit (Ambion) to eliminate potential genomic DNA contamination. RNA concentration and integrity were assessed using a NanoDrop ND-1000 spectrophotometer and an Agilent 2100 Bioanalyzer, respectively. Total RNA with RNA integrity (RIN) scores >= 9 were retained for RNA sequencing. For each replicate, total RNA was extracted from 50-58 JcDV-infected larvae.
characteristics.characteristic: description
characteristics.text: larvae stage 5
characteristics.characteristic: dev_stage
characteristics.text: JcDV
characteristics.characteristic: infection
characteristics.text: Spodoptera frugiperda
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7108
characteristics.characteristic: organism
characteristics.text: biological replicate 3
characteristics.characteristic: replicate
characteristics.text: tissue sample
characteristics.characteristic: sample_type
characteristics.text: corn
characteristics.characteristic: strain
characteristics.text: hemocyte
characteristics.characteristic: tissue
characteristics.text: Spodoptera frugiperda hemocyte
characteristics.characteristic: title