characteristics.text: SAMN31888822
characteristics.characteristic: External Id
characteristics.text: Saiyong Zhu's lab, Life Sciences Institute, Zhejiang University
characteristics.characteristic: INSDC center name
characteristics.text: 2023-05-03T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-05-03T07:37:23.446Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS15880319
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS15880319
characteristics.characteristic: SRA accession
characteristics.text: Reprogramming intermediate
characteristics.characteristic: cell type
characteristics.text: 129XOG2
characteristics.characteristic: genotype
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Reprogramming intermediate
characteristics.characteristic: source_name
characteristics.text: D4, scRNAseq
characteristics.characteristic: title
characteristics.text: iPSCs were seeded on Feeder and maintained in mESC medium. mESC medium contains 47% DMEM/F12, 47% Neural basal medium, 1 x N2 supplement, 1 x B27 supplement, 1% Glutamax, 1% nonessential amino acids (NEAA), 0.055 mM 2-mercaptoethanol (β-ME), 1% P/S, 1000 units/mL mLIF, 3 μM CHIR99021 and 0.2 μM PD0325901. MEFs were cultured in MEF medium, containing DMEM Basic, 10% fetal bovine serum (FBS), 1% penicillin and streptomycin (P/S). OG2 MEFs of passages 1-3 were seeded into 6-well plates at density of 2.5 x 10^4 cells/plate. On the next day, medium was changed to reprogramming medium. Medium was changed every 2 days. For reprogramming day 4 to 8, the medium was renewed every day.
characteristics.characteristic: treatment