characteristics.text: SAMN31853184
characteristics.characteristic: External Id
characteristics.text: Department of Molecular Medicine, University of Padua
characteristics.characteristic: INSDC center name
characteristics.text: 2023-02-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-02-02T04:42:25.343Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS15853660
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS15853660
characteristics.characteristic: SRA accession
characteristics.text: primed induced pluripotent stem cells
characteristics.characteristic: cell type
characteristics.text: Cells were cultured in Feeder-free on pre-coated plates with 0.5% growth factor-reduced Matrigel (CORNING 356231) (in PBS with MgCl2/CaCl2, Sigma-Aldrich D8662) in E8 medium (made in-house according to12) at 37°C, 5% CO2, 5% O2. Cells were passaged every 3-4 days at a split ratio of 1:8 following dissociation with 0.5 mM EDTA (Invitrogen AM99260G) in PBS without MgCl2/CaCl2 (Sigma-Aldrich D8662), pH8.
characteristics.characteristic: growth protocol
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: KiPS primed-iPSCs
characteristics.characteristic: source_name
characteristics.text: KiPS_Rep_1
characteristics.characteristic: title