characteristics.text: SAMN31976376
characteristics.characteristic: External Id
characteristics.text: The University of Sydney
characteristics.characteristic: INSDC center name
characteristics.text: 2023-01-31T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-01-31T11:23:02.148Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS15936795
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Human
characteristics.characteristic: NCBI submission model
characteristics.text: Human.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS15936795
characteristics.characteristic: SRA accession
characteristics.text: 47
characteristics.characteristic: age
characteristics.text: NIH NeuroBioBank
characteristics.characteristic: biomaterial_provider
characteristics.text: Background: DNA methylation is a critical molecular mark involved in cellular differentiation and cell-specific processes. Single-cell whole genome DNA methylation profiling methods hold great potential to resolve the DNA methylation profiles of individual cell-types. Here we present a method that couples single-cell combinatorial indexing (sci) with enzymatic conversion (sciEM) of unmethylated cytosines. Results: The sciEM method facilitates DNA methylation profiling of single-cells that is highly correlated with single-cell bisulfite-based workflows (r2 >0.99) whilst improving sequencing alignment rates, reducing adapter contamination and over-estimation of DNA methylation levels (CpG and non-CpG). As proof-of-concept we perform sciEM analysis of the temporal lobe, motor cortex, hippocampus and cerebellum of the human brain to resolve single-cell DNA methylation of all major cell-types. Conclusion: To our knowledge sciEM represents the first non-bisulfite single-cell DNA methylation sequencing approach with single-base resolution.
characteristics.characteristic: description
characteristics.text: 47-year-old female donor with no diagnosis of neurological disease
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: Brain
characteristics.characteristic: tissue
characteristics.text: Single-cell DNA methylation sequencing by combinatorial indexing and enzymatic DNA methylation conversion.
characteristics.characteristic: title