characteristics.text: SAMN32928079
characteristics.characteristic: External Id
characteristics.text: University of California at Berkeley
characteristics.characteristic: INSDC center name
characteristics.text: 2023-03-23T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-03-23T17:56:02.244Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS16599367
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Microbe, viral or environmental
characteristics.characteristic: NCBI submission model
characteristics.text: Microbe.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS16599367
characteristics.characteristic: SRA accession
characteristics.text: yes
characteristics.characteristic: UMI
characteristics.text: Nicholas Ingolia
characteristics.characteristic: biomaterial_provider
characteristics.text: 100 nt to 120 nt
characteristics.characteristic: cdna_size_selection
characteristics.text: Culture 4
characteristics.characteristic: cell_lysate_batch
characteristics.text: sucrose cushion
characteristics.characteristic: cushion_gradient
characteristics.text: 250 ng/ml of anhydrotetracycline, biological replicate 1, mirRICH small RNA enrichment, cDNA size-selection from 100 nt to 120 nt RNA size-selection, mRNA digested by P1 nuclease, library generated by OTTR, F753-labeling, with UMI
characteristics.characteristic: description
characteristics.text: HTS1
characteristics.characteristic: knock_down
characteristics.text: OTTR, F753-labeling, with UMI
characteristics.characteristic: library_method
characteristics.text: P1 nuclease
characteristics.characteristic: nuclease_digestion
characteristics.text: Saccharomyces cerevisiae
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4932
characteristics.characteristic: organism
characteristics.text: biological 1
characteristics.characteristic: replicate
characteristics.text: mirRICH enrichment
characteristics.characteristic: rna_size_selection
characteristics.text: Ribosome profiling of cell lysate from log-phase growth yeast cell culture
characteristics.characteristic: sample_type
characteristics.text: gcn2(delta) dCas9-Mxi1::TetR::kanMXsyn P(RPR1)Tet-HTS1sgRNA::KlLEU2 log-phase growth lysate
characteristics.characteristic: strain
characteristics.text: 30 °C
characteristics.characteristic: temp
characteristics.text: mirRICH small RNA enrichment and cDNA size-selection of P1 nuclease digested RPFs from a sucrose cushion pellet, biological replicate 1 of anhydrotetracycline treated cells (HTS1 knock-down)
characteristics.characteristic: title