characteristics.text: 2020-10-13
characteristics.characteristic: Date_sequenced
characteristics.text: Cap-seq
characteristics.characteristic: Experiment_type
characteristics.text: SAMN32942865
characteristics.characteristic: External Id
characteristics.text: University of Chicago
characteristics.characteristic: INSDC center name
characteristics.text: 2023-01-27T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-01-27T18:01:15.037Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS16610880
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Model organism or animal
characteristics.characteristic: NCBI submission model
characteristics.text: Model.organism.animal.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: empty vector (control for ints-1 treatment)
characteristics.characteristic: RNAi treatment
characteristics.text: SRS16610880
characteristics.characteristic: SRA accession
characteristics.text: Total RNA was extracted from whole animals of ~100,000 synchronized young adults. Terminator exonuclease (Epicentre) was used to selectively degrade monophosphorylated RNAs. Quick CIP (New England Biolabs) was used to dephosphorylate non-capped triphosphorylated RNAs. RppH was used with Thermopol Buffer (New England Biolabs) to decap RNAs for ligation. Libraries were prepared according to the manufacturer’s protocol of the NEBNext Multiplex Small RNA Sample Prep Set for Illumina-Library Preparation (New England Biolabs). NEBNext Multiplex Oligos for Illumina Index Primers were used for library preparation (New England Biolabs). Libraries were sequenced using an Illumina NovaSeq6000 to obtain single-end 100 nt sequences at the University of Chicago Genomic Facility.
characteristics.characteristic: description
characteristics.text: young adult
characteristics.characteristic: dev_stage
characteristics.text: oma-1::gfp I; cdk-1::gfp II; 21ur-anti-gfp(type I) IV.
characteristics.characteristic: genotype
characteristics.text: Staged worms were grown from L1 to YA at 20C on empty vector RNAi food
characteristics.characteristic: growth_protocol
characteristics.text: Caenorhabditis elegans
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6239
characteristics.characteristic: organism
characteristics.text: 1
characteristics.characteristic: replicate
characteristics.text: hermaphrodite
characteristics.characteristic: sex
characteristics.text: HCL134
characteristics.characteristic: strain
characteristics.text: whole worm
characteristics.characteristic: tissue
characteristics.text: control_capseq.fastq.gz
characteristics.characteristic: title