characteristics.text: SAMN32983304
characteristics.characteristic: External Id
characteristics.text: Sun Yat-sen University
characteristics.characteristic: INSDC center name
characteristics.text: 2023-01-31T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-02-02T12:34:09.407Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS16658258
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Human
characteristics.characteristic: NCBI submission model
characteristics.text: Human.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS16658258
characteristics.characteristic: SRA accession
characteristics.text: The human DLD1 colorectal cancer (CRC) cell line was obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA) and cultured according to standard guidelines. All cells were authenticated by short tandem repeat fingerprinting before use at the Medicine Laboratory of the Forensic Medicine Department of Sun Yat-sen University (Guangzhou, China). All plasmids with the Flag-tag were provided by Saisofi Biotechnology Co., Ltd. (Jiangsu, China) and transfected into the cells using ViaFect Transfection Reagent (E4982, Promega, Madison, WI, USA) according to the recommended protocol. The lentiviruses packaging wild-type PTEN and site-directed mutants were synthesized by OBiO Technology (Shanghai, China). CRC cells were transfected with lentiviruses and selected with puromycin (HY-B1743A, MedChemExpress, NJ, USA) for 7 days.
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: colorectal cancer
characteristics.characteristic: tissue
characteristics.text: PTEN WT and R14M mutation DLD1 cell line
characteristics.characteristic: title