characteristics.text: SAMN33428178
characteristics.characteristic: External Id
characteristics.text: The University of Queensland
characteristics.characteristic: INSDC center name
characteristics.text: 2023-04-27T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-04-27T21:20:02.759Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS17411480
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Model organism or animal
characteristics.characteristic: NCBI submission model
characteristics.text: Model.organism.animal.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS17411480
characteristics.characteristic: SRA accession
characteristics.text: 8 weeks
characteristics.characteristic: age
characteristics.text: EGF negative niche cell
characteristics.characteristic: cell_type
characteristics.text: 2022-05
characteristics.characteristic: collection_date
characteristics.text: Primary cells isolated from the dentate gyrus of 8-week-old C57BL/6J mice were treated with Saline. After 1.5 h, the cells were stained with epidermal growth factor (EGF) complexed to AlexaFluor 647 streptavidin to label neural stem cells. EGF- neural stem cells were collected using flow cytometry, followed by immediate RNA collection. cDNA was prepared, and a library preparation performed, before the samples were sequenced using the Illumina Nova Seq 6000 sequencing system.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: dev_stage
characteristics.text: 17
characteristics.characteristic: isolate
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Primary cells
characteristics.characteristic: sample_type
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: C57BL/6J
characteristics.characteristic: strain
characteristics.text: Dentate gyrus primary cells
characteristics.characteristic: tissue
characteristics.text: EGF negative niche cells from the mouse dentate gyrus
characteristics.characteristic: title
characteristics.text: Saline
characteristics.characteristic: treatment