characteristics.text: SAMN33569904
characteristics.characteristic: External Id
characteristics.text: Rene Ketting group
characteristics.characteristic: INSDC center name
characteristics.text: 2023-03-14T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-03-14T15:30:02.768Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS16949047
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: BmAGO3
characteristics.characteristic: IP_target
characteristics.text: Model organism or animal
characteristics.characteristic: NCBI submission model
characteristics.text: Model.organism.animal.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS16949047
characteristics.characteristic: SRA accession
characteristics.text: B
characteristics.characteristic: biological_replicate
characteristics.text: Per condition, one well of a 6-well plate was seeded with 6 × 105 BmN4 cells 24h prior to transfection with either HA-eGFP, HA-BmAgo3, HA-Siwi or HA-BmGtsf1L using X-tremeGene HP transfection reagent. 72h post transfection, cells were harvested and an anti-HA immunoprecipitation was performed as described above, the experiment was performed in duplicate. Immunopurified RNAs were extracted from beads by adding 1 mL Trizol LS (Invitrogen #10296028), according to the manufacturer’s instructions. The lysate was incubated at RT for 5 min to allow complete dissociation of the nucleoprotein complex. Next, 200 µL of chloroform was added to 1 mL of lysate followed by harsh mixing and centrifugation at 12,000×g for 15 min at 4°C. Another round of chloroform extraction was performed and the aqueous phase was transferred to a fresh tube to which 1 volume (500 µL) of ice-cold isopropanol was added for RNA precipitation. RNA pellets were washed twice in 1 mL of 70% ice-cold ethanol and centrifuged at 7,500×g for 10 min at 4°C. The RNA pellet was air-dried and dissolved in nuclease-free water.
characteristics.characteristic: description
characteristics.text: undefined
characteristics.characteristic: dev_stage
characteristics.text: IP
characteristics.characteristic: fraction
characteristics.text: Bombyx mori
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7091
characteristics.characteristic: organism
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: BmN4
characteristics.characteristic: strain
characteristics.text: ovary
characteristics.characteristic: tissue
characteristics.text: BmAGO3_HA_IP_B
characteristics.characteristic: title