characteristics.text: SAMN33812955
characteristics.characteristic: External Id
characteristics.text: Weizmann Institute of Science
characteristics.characteristic: INSDC center name
characteristics.text: 2023-03-18T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-03-18T22:06:34.563Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS17085697
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Human
characteristics.characteristic: NCBI submission model
characteristics.text: Human.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS17085697
characteristics.characteristic: SRA accession
characteristics.text: HEK-293T
characteristics.characteristic: cell_line
characteristics.text: Human ADAR (ADAR1) knockout HEK-293T
characteristics.characteristic: cell_subtype
characteristics.text: ADAR1-knockout HEK293T cells were grown (37°C, 5% CO2) in Gibco Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum, 1% Penicillin and Streptomycin, and 4ug/ml Puromycin. 500000 cells were plated on a 6-well plate so that cells reached 70-90% confluency at the time of the second transfection. 24 and 48 hours after cell seeding, 1.6μg of No_ADAR-expressing pcDNA3.1(-) plasmid (Negative Control) and 4μg B2 library DNA were transfected respectively according to Lipofectamine® 2000 DNA Transfection Reagent Protocol (Thermo Fisher Scientific). 24 hours later, cell harvesting was performed. The upper constant arms including 8 nucleotide barcodes of constructs were reverse transcribed, PCR amplified (primers in Supplementary Table 1), and sequenced using NovaSeq 6000 SP Reagent Kit v1.5 (300 cycles).
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: B2_library_No_ADAR_overexpression_ADAR1_KO_HEK_293T_2
characteristics.characteristic: title
characteristics.text: B2 library along with No_ADAR overexpression Replicate2
characteristics.characteristic: treatment