characteristics.text: SAMN33818909
characteristics.characteristic: External Id
characteristics.text: Weizmann Institute of Science
characteristics.characteristic: INSDC center name
characteristics.text: 2023-03-19T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-03-20T19:57:48.160Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS17086461
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Human
characteristics.characteristic: NCBI submission model
characteristics.text: Human.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS17086461
characteristics.characteristic: SRA accession
characteristics.text: HEK-293T
characteristics.characteristic: cell_line
characteristics.text: Human ADAR (ADAR1) knockout HEK-293T
characteristics.characteristic: cell_subtype
characteristics.text: ADAR1-knockout HEK293T cells were grown (37°C, 5% CO2) in Gibco Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum, 1% Penicillin and Streptomycin, and 4ug/ml Puromycin. 500000 cells were plated on a 6-well plate so that cells reached 70-90% confluency at the time of transfection. 24 hours later, 1ug of ADAR2-expressing pcDNA3.1(-) plasmid, 0.1ug of pEGFP-N1 plasmid, and 3ug of the corresponding arRNA-expressing plasmid were transfected according to Lipofectamine® 2000 DNA Transfection Reagent Protocol. 24 hours later, the medium was changed, and 12 hours later, cells were harvested. Total RNA was extracted using Nucleozol (Macherey-Nagel), and DNase treated (Thermo Fisher Scientific) and reverse transcribed using MultiScribe Reverse Transcriptase cDNA synthesis kit (Thermo Fisher Scientific). The subsequent PCR with KAPA HiFi HotStart ReadyMix (Roche) was performed using GAPDH transcript-specific primers, and sequenced on the Illumina Novaseq platform.
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Mismatch27_GAPDH_arRNA_ADAR2_overexpression_ADAR1_KO_HEK293T_1
characteristics.characteristic: title
characteristics.text: ADAR-recruiting RNAs (arRNA) were designed to elicit editing on endogenous GAPDH transcripts. The Mismatch27 arRNA, which contains a C opposite to the target adenosine and an extra 3-bp mismatch 27 nt upstream from editing site, was transfected into ADAR2-expressing ADAR1-KO HEK293T cells. (Replicate1)
characteristics.characteristic: treatment