characteristics.text: SAMN33863941
characteristics.characteristic: External Id
characteristics.text: University of Kansas
characteristics.characteristic: INSDC center name
characteristics.text: 2023-03-22T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-03-23T18:46:15.240Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS17124611
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: MIMARKS.survey
characteristics.characteristic: NCBI submission model
characteristics.text: MIGS/MIMS/MIMARKS.soil
characteristics.characteristic: NCBI submission model
characteristics.text: MIMARKS.survey.soil.6.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS17124611
characteristics.characteristic: SRA accession
characteristics.text: 19.44 in
characteristics.characteristic: annual_precpt
characteristics.text: 16S_SVR_inocula_Ag_3
characteristics.characteristic: biological_replicate
characteristics.text: 2019-06-25
characteristics.characteristic: collection_date
characteristics.text: Slurries were prepared by mixing 20 g of each soil in separate glass bottles of 100 mL 1x phosphate-buffered saline (PBS) and 0.0001% Triton X-100. Samples were allowed to settle and then filtered through Miracloth (22-25 µm pore size; Calbiochem, San Diego, CA, USA) and decanted into 50 mL centrifuge tubes. Samples were centrifuged for 30 minutes (3000 x g) and the supernatant was poured off. The resulting pellet was resuspended in 20 mL of 1x PBS. Samples were stored in aliquots and kept shaking for aeration until use.
characteristics.characteristic: collection_method
characteristics.text: Maize/Soybean
characteristics.characteristic: crop_rotation
characteristics.text: 3-60 cm
characteristics.characteristic: depth
characteristics.text: Keywords: GSC:MIxS;MIMARKS:6.0
characteristics.characteristic: description
characteristics.text: 884 m
characteristics.characteristic: elev
characteristics.text: temperate biome [ENVO:01001831]
characteristics.characteristic: env_broad_scale
characteristics.text: agricultural field [ENVO:00000114]
characteristics.characteristic: env_local_scale
characteristics.text: slurry [ENVO:03600017]|soil [ENVO:00001998]
characteristics.characteristic: env_medium
characteristics.text: USA: Kansas, Smoky Valley Ranch
characteristics.characteristic: geo_loc_name
characteristics.text: Soil slurry
characteristics.characteristic: isolation_source
characteristics.text: 38.8791 N 100.9828 W
characteristics.characteristic: lat_lon
characteristics.text: soil metagenome
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_410658
characteristics.characteristic: organism
characteristics.text: 800 uL
characteristics.characteristic: samp_vol_we_dna_ext
characteristics.text: Prepared fresh on day of extraction
characteristics.characteristic: sieving
characteristics.text: 16S_51
characteristics.characteristic: title