SAMN35218432
External Id
Duke University
INSDC center name
2023-05-22T00:00:00Z
INSDC first public
2023-05-22T09:58:04.102Z
INSDC last update
SRS17747515
INSDC secondary accession
live
INSDC status
Microbe, viral or environmental
NCBI submission model
Microbe.1.0
NCBI submission package
SRS17747515
SRA accession
MNase-seq
assay_type
2021-03-10
collection_date
Yeast cells were grown at 25C to OD600 of ~0.7. EdU was added to the culture at a final concentration of 130 M. At the same time, -factor was added to the culture at a final concentration of 50 ng/mL. After 5 minutes of EdU pulse, a sample was taken for nascent chromatin, after which cells were washed and resuspended in fresh media containing 130 M thymidine and 50 ng/mL -factor. Samples were taken at 10, 15, 20, and 40 minutes after the media change.
description
MATa, leu2-3,112, BAR1::TRP, can1-100, URA3::BrdU-Inc, ade2-1, his3-11,15, rlf2::HIS
genotype
USA
geo_loc_name
a
mating_type
Saccharomyces cerevisiae
http://purl.obolibrary.org/obo/NCBITaxon_4932
organism
1
replicate
Cell culture
sample_type
cac1
strain
25
temp
5 pulse
time_point
MNase-seq of cac1, 5 min pulse, replicate 1
title