SAMN30338788
External Id
Peking University
INSDC center name
2023-05-17T00:00:00Z
INSDC first public
2023-05-17T07:36:03.732Z
INSDC last update
SRS14672760
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
SRS14672760
SRA accession
mES V6.5
cell type
RAD21-mAID-mClover
genotype
Mus musculus
http://purl.obolibrary.org/obo/NCBITaxon_10090
organism
cultured cell
source_name
mES_RAD21_mAC_1C4_NT_EdUHU
title
Cells were incubated with 1.25 mM thymidine (Sigma, T9250) as well as 1 mg/mL dox for 14 hours, followed by dox and 50 ng/mL nocodazole (Sigma, M1404) for 7 hours to synchronize into M phase. After synchronization, mES cells were released into a fresh medium with 4 mM HU for 3.5 hours.
treatment