SAMN32949897
External Id
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
INSDC center name
2023-05-19T00:00:00Z
INSDC first public
2023-05-19T08:33:03.324Z
INSDC last update
SRS16615102
INSDC secondary accession
live
INSDC status
Model organism or animal
NCBI submission model
Model.organism.animal.1.0
NCBI submission package
SRS16615102
SRA accession
6 months
age
replicate 2
biological_replicate
Bloomington, USA
biomaterial_provider
D. melanogaster wild type Canton-S line (#64349, Bloomington, USA) was used in the experiments. Newly eclosed flies were collected and reared in food medium containing 1000 ml water, 7 g agar, 8 g yeast, 30 g sugar, 30 g semolina, and 3 ml propionic acid. Males and females were kept separately in a Binder KBF720-ICH climate chamber (Binder) at 25°C and 60% humidity, with light/dark alternation (12h/12h). Flies were kept in narrow Drosophila vials (Genesee Scientific) at a density of 30 individuals per vial, 5 vials per experimental and control groups. Flies were transferred to the fresh medium twice a week. Flies were kept on the nutrient medium spread with the past of hydrolyzed yeast without Torin-2. Dead flies were coted daily. Heads were manually separated from the bodies and immediately frozen in liquid nitrogen. Three replications of the experiment for each group were performed.
description
wild type
genotype
Drosophila melanogaster
http://purl.obolibrary.org/obo/NCBITaxon_7227
organism
whole heads
sample_type
male
sex
wild type Canton-S line (#64349, Bloomington, USA)
strain
whole heads
tissue
D. melanogaster heads, male 6-months, control, replicate 2
title
no treatment
treatment