SAMN35218454
External Id
Duke University
INSDC center name
2023-05-22T00:00:00Z
INSDC first public
2023-05-22T09:58:04.316Z
INSDC last update
SRS17747537
INSDC secondary accession
live
INSDC status
Microbe, viral or environmental
NCBI submission model
Microbe.1.0
NCBI submission package
SRS17747537
SRA accession
RNA-seq
assay_type
2023-01-31
collection_date
Yeast cells were grown at 25C to an OD600 of ~ 0.7 and arrested in G1 phase with -factor at a final concentration of 50 ng/mL for 2 h. At the end of -factor arrest, a sample was collected (af arrested), and then the cells were washed twice in sterile water and released into fresh media. Samples were collected at 10-minute intervals for 60 minutes, after which -factor was added back to the media at the same concentration. A final sample was collected at 150 minutes after the initial release from -factor.
description
MATa, leu2-3,112, BAR1::TRP, can1-100, URA3::BrdU-Inc, ade2-1, his3-11,15, rlf2::HIS
genotype
USA
geo_loc_name
a
mating_type
Saccharomyces cerevisiae
http://purl.obolibrary.org/obo/NCBITaxon_4932
organism
1
replicate
Cell culture
sample_type
cac1
strain
25
temp
40
time_point
RNA-seq of cac1, 40 min after release from aF, replicate 1
title