SAMN35341209
External Id
University of Waterloo
INSDC center name
2023-05-23T00:00:00Z
INSDC first public
2023-05-24T11:54:02.843Z
INSDC last update
live
INSDC status
MIMAG.water
NCBI submission model
MIMAG.water.6.0
NCBI submission package
2017-09
collection_date
3.88 m
depth
This BioSample is a metagenomic assembly obtained from the Ca. Chlorohelix allophototropha enrichment, subculture 15.c metagenome BioSample: SAMN35341089. Assembled contigs were polished using short read data from BioSample: SAMN15312057.
derived-from
Keywords: GSC:MIxS;MIMAG:6.0
description
freshwater lake biom [ENVO:01000252]
env_broad_scale
holomictic lake [ENVO:01000285]|humic lake [ENVO:01001021]|anoxic lake [ENVO:01001072]
env_local_scale
fresh water [ENVO:00002011]
env_medium
1
environmental-sample
Canada: near Kenora, Ontario, Lake 227, IISD-Experimental Lakes Area
geo_loc_name
L227-G1
isolate
Candidatus Chlorohelix allophototropha enrichment, subculture 15.c; a non-axenic enrichment culture of water from the upper anoxic zone (3.88 m depth) of a Boreal Shield lake
isolation_source
49.69 N 93.69 W
lat_lon
freshwater metagenome
metagenome-source
1
metagenomic
Geothrix sp.
http://purl.obolibrary.org/obo/NCBITaxon_1962974
organism
anaerobe
rel_to_oxygen
Anoxic water was pumped to the surface through a flushed line using a gear pump. Water was pumped directly into 120 mL glass serum bottles that were allowed to overflow for several seconds and then quickly sealed using rubber stoppers.
samp_collect_device
Water was kept chilled during storage and shipping to the laboratory. Water was then distributed anoxically into glass serum bottles, amended with a ferrous iron-containing freshwater minimal medium, and incubated under light.
samp_mat_process
metagenomic assembly
sample-type
Closed circular metagenome-assembled genome from Geothrix strain L227-G1
title