SAMN35218461
External Id
Duke University
INSDC center name
2023-05-22T00:00:00Z
INSDC first public
2023-05-22T09:58:04.697Z
INSDC last update
SRS17747544
INSDC secondary accession
live
INSDC status
Microbe, viral or environmental
NCBI submission model
Microbe.1.0
NCBI submission package
SRS17747544
SRA accession
RNA-seq
assay_type
2021-10-11
collection_date
Yeast cells were grown at 25C to an OD600 of ~ 0.7 and arrested in G1 phase with -factor at a final concentration of 50 ng/mL for 2 h. At the end of -factor arrest, a sample was collected (af arrested), and then the cells were washed twice in sterile water and released into fresh media. Samples were collected at 40 and 60 minutes after af release.
description
MATa, leu2-3,112, BAR1::TRP, can1-100, URA3::BrdU-Inc, ade2-1, his3-11,15, rlf2::HIS
genotype
USA
geo_loc_name
a
mating_type
Saccharomyces cerevisiae
http://purl.obolibrary.org/obo/NCBITaxon_4932
organism
2
replicate
Cell culture
sample_type
cac1
strain
25
temp
0
time_point
RNA-seq of cac1, alpha facter arrested, replicate 2
title