SAMN34998568
External Id
University of Portsmouth
INSDC center name
2023-05-27T06:42:19.753Z
INSDC first public
2023-05-27T06:42:19.753Z
INSDC last update
SRS17806627
INSDC secondary accession
live
INSDC status
Model organism or animal
NCBI submission model
Model.organism.animal.1.0
NCBI submission package
SRS17806627
SRA accession
2015
collection_date
Embryos at HH7/8 each were prepared for roller-tube culture as described in Dupe and Lumsden (2001). Batches of 5 embryos were transferred into sterile 10 ml glass vials containing 2 ml of culture medium (L15 Leibovitz, 10% chicken serum, 0.05 mg/ml gentamicin), supplemented with DMSO (1:1000). The embryos were cultured in a rotary hybridisation oven at 38 degrees Celsius for 6 hours. Thereafter, embryos that had developed normally were collected, and the whole head region up to the 1st somite was harvested in RNALater. For each replicate, total RNA was prepared from 10-15 embryos using the Absolutely RNA Microprep kit (Agilent).
description
HH8/9
dev_stage
United Kingdom
geo_loc_name
Gallus gallus
http://purl.obolibrary.org/obo/NCBITaxon_9031
organism
embryo culture
sample_type
not determined
sex
Shaver Brown
strain
embryonic head
tissue
Chicken embryonic head at HH8/9 after 6-hour culture with DMSO (1:1000) as solvent control
title
DMSO (1:1000) for 6 hours
treatment