SAMN24256409
External Id
Free University of Bolzano
INSDC center name
2023-06-30T00:50:22.967Z
INSDC first public
2023-06-30T00:50:22.967Z
INSDC last update
SRS11360391
INSDC secondary accession
live
INSDC status
Metagenome or environmental
NCBI submission model
Metagenome.environmental.1.0
NCBI submission package
SRS11360391
SRA accession
2019
collection_date
A 464-nucleotide sequence of the V3-V4 region of the 16S rRNA gene (Escherichia coli positions 341 to 805) was amplified starting from DNA. Unique barcodes were attached before the forward primers. The amplicons were cleaned using the Agencourt AMPure kit (Beckman coulter). DNA concentrations of the amplicons were determined using the Quant-iT PicoGreen dsDNA kit (Invitrogen). The quality of the generated amplicon libraries was evaluated by a Bioanalyzer 2100 (Agilent, Palo Alto, CA, USA) using the High Sensitivity DNA Kit (Agilent).
design_description
BD0-S1_R1.fastq
filename
BD0-S1_R2.fastq
filename2
fastq
filetype
Italy
geo_loc_name
Tritordeum
host
Illumina MiSeq
instrument_model
dough
isolation_source
46.49517 N 11.35378 E
lat_lon
BD0-S1
library_id
paired
library_layout
PCR
library_selection
METAGENOMIC
library_source
AMPLICON
library_strategy
plant metagenome
http://purl.obolibrary.org/obo/NCBITaxon_1297885
organism
ILLUMINA
platform
Illumina 16S sourdough
title
Metagenome or environmental sample from plant metagenome
title