NA
Additional.info.affecting.microbiome..M3...Biospecimen.
40001-80000
Annual.household.income..Biospecimen.
True
Anti.infective..Biospecimen.
NA
Autism.spectrum.disorder..Biospecimen.
0.729
Average.conc..Extract.
batch6
Batch..OmicsFileSet.
True
Behavior.video.submitted..M3...Biospecimen.
B18012407
Biospecimen.Barcode
2018-02-05T00:00:00
Biospecimen.Date.Collected
Matched with metadata entry ASD_T3_Family_120
Biospecimen.Description
152894
Biospecimen.ID
M3_120_P1_A_V1
Biospecimen.Name
0.25
Biospecimen.consumption
gram
Biospecimen.consumption.unit
NA
Biospecimen.location..Extract.
NA
Biospecimen.mass
NA
Biospecimen.mass.unit
True
Born.by.caesarian.section..Biospecimen.
False
Born.prematurely..Biospecimen.
7-10 meals per week
Bread..consumption.frequency...longitudinal...Biospecimen.
Developmentally delayed (autism)
Childhood.behavioral.development.finding..Biospecimen.
0.729
Conc1..Extract.
0.729
Conc2..Extract.
False
Constipation..Biospecimen.
Cannot have a conversation
Conversation.ability..Biospecimen.
Daily
Dairy..consumption.frequency...Biospecimen.
Almost every meal
Dairy..consumption.frequency...longitudinal...Biospecimen.
False
Diarrhea..Biospecimen.
Mixed infant formula and mother's milk diet
Diet.during.infancy..Biospecimen.
Never or less than once per week
Dietary.fat.and.oil..consumption.frequency...longitudinal...Biospecimen.
Omnivorous diet
Dietary.regime..Biospecimen.
False
Dietary.restrictions..M3...Biospecimen.
NA
Dietary.restrictions.details..M3...Biospecimen.
True
Dietary.supplement..Biospecimen.
True
Environmental.tobacco.smoke.exposure..Biospecimen.
NA
Explain.how.you.collected.stool.sample..M3...Biospecimen.
SAMN31514741
External Id
Z18010568
Extract.Barcode
NA
Extract.Date.Collected
156245
Extract.ID
NA
Extract.Name
0.1458
Extract.concentration
ng/microliter
Extract.concentration.unit
NA
Extract.consumption
NA
Extract.consumption.unit
100
Extract.volume
uL
Extract.volume.unit
NA
Extraction.Protocol.description
Glass
Extraction.Protocol.disruption_bead_type
600
Extraction.Protocol.disruption_duration
1750
Extraction.Protocol.disruption_intensity
NA
Extraction.Protocol.disruption_temperature
2018-05-10T18:01:55.521Z
Extraction.Protocol.dtstamp
gDNA
Extraction.Protocol.extract_type
MagAttract PowerMicrobiome DNA/RNA KF
Extraction.Protocol.extraction_kit
RNAse-Free Water
Extraction.Protocol.final_buffer
316
Extraction.Protocol.id
MAD LAB High Throughput Extraction of DNA using the MagAttract PowerMicrobiome DNA/RNA KF Kit
Extraction.Protocol.name
NA
Extraction.Protocol.notes
100
Extraction.Protocol.nucleic_acid_eluted_volume
452
Extraction.Protocol.protocol_id
Magnetic Beads
Extraction.Protocol.purification_method
-20
Extraction.Protocol.storage_temperature
24
Extraction.Protocol.user_id
KF028_B
Extraction.batch.ID..Extract.
NA
Extraction.kit.lot.number..Extract.
Consistent eye contact
Eye.contact.finding..Biospecimen.
120
Family.group.ID..Biospecimen.
Several times weekly
Fermented.vegetable..consumption.frequency...Biospecimen.
False
Fever..Biospecimen.
False
Flu.like.symptoms..Biospecimen.
6 months
Flu.shot.in.the.last..M3...Biospecimen.
NA
Flu.shot.in.the.last..MFlu.shot.in.the.last..M3...Biospecimen.
NA
Foreign.travel.within.past.months..Biospecimen.
Rarely
Frequency.of.interaction.with.pet.s...Biospecimen.
Daily
Fruit..consumption.frequency...Biospecimen.
Almost every meal
Fruit..consumption.frequency...longitudinal...Biospecimen.
Tends to have normal bowel function
Functional.bowel.finding..Biospecimen.
True
GI.issues.one.month.ago..M3...Biospecimen.
False
GI.issues.this.week..M3...Biospecimen.
False
GI.issues.three.months.ago..M3...Biospecimen.
False
GI.issues.two.months.ago..M3...Biospecimen.
False
GI.issues.two.weeks.ago..M3...Biospecimen.
True
GI.symptoms.within.3.months..M3...Biospecimen.
Sometimes
Gastrointestinal.problems..M3...Biospecimen.
NA
Gestational.age..Biospecimen.
False
Gluten.allergy..Biospecimen.
9471
Host.ID
120_A
Host.Name
Case (disease status)
Host.disease.status..Biospecimen.
True
Host.interaction.with.pets..Biospecimen.
Oregon State University
INSDC center name
2023-07-01T00:00:00Z
INSDC first public
2023-07-01T13:42:54.762Z
INSDC last update
SRS15613742
INSDC secondary accession
live
INSDC status
Imitates others when prompted
Imitation.behavior..Biospecimen.
True
Indoors.pet..Biospecimen.
NA
Indoors.pets..Biospecimen.
NA
LR.L2.2.Video.classifier.score..M3...Biospecimen.
False
Lactose.intolerance..Biospecimen.
Little to no speech
Language.ability.and.use..Biospecimen.
NA
Length.of.recent.foreign.travel..Biospecimen.
L18008039
Library.Barcode
NA
Library.Date.Collected
156329
Library.ID
NA
Library.Name
3483.4
Library.concentration
ng/uL
Library.concentration.unit
NA
Library.mass
NA
Library.mass.unit
50
Library.volume
microliter
Library.volume.unit
C01
Location.on.Extraction.Plate..Extract.
Daily
Meals.prepared.at.home..consumption.frequency...Biospecimen.
Rarely
Meat..consumption.frequency...Biospecimen.
7-10 meals per week
Meats.and.seafood..consumption.frequency...longitudinal...Biospecimen.
2
Minimum.time.since.antibiotics..Biospecimen.
-8.034
Mobile.Autism.Risk.Assessment.Score..Biospecimen.
Diarrhea with vomiting & Fever
Most.recent.GI.episode.symptoms..M3...Biospecimen.
True
Multivitamin..Biospecimen.
Human
NCBI submission model
Human.1.0
NCBI submission package
587
Natural.father.current.age..months...Biospecimen.
527
Natural.mother.current.age..months...Biospecimen.
120_N
Natural.sibling.included.in.present.study..Biospecimen.
False
Non.celiac.gluten.sensitivity..Biospecimen.
NA
Normalized.concentration..Extract.
0
Number.of.pet.birds..Biospecimen.
1
Number.of.pet.cats..Biospecimen.
0
Number.of.pet.dogs..Biospecimen.
3
Number.of.pet.fish..Biospecimen.
0
Number.of.pet.horses..Biospecimen.
0
Number.of.pet.reptiles..Biospecimen.
0
Number.of.roommates..Biospecimen.
Daily
Olive.oil.used.in.cooking..M3...Biospecimen.
L18008039_M05416_40_000000000_BTH43
OmicsFileSet.Barcode
157683
OmicsFileSet.ID
_O1
OmicsFileSet.Name
False
Other.GI.symptoms..M3...Biospecimen.
False
Other.symptoms.this.week..M3...Biospecimen.
False
Outdoors.pet..Biospecimen.
NA
Outdoors.pets..Biospecimen.
0
PCR.Protocol.BSA_final_conc
0
PCR.Protocol.KCl_final_conc
0
PCR.Protocol.MgCl2_final_conc
0
PCR.Protocol.MgSO4_final_conc
0
PCR.Protocol.Tris_HCl_final_conc
0
PCR.Protocol.Tris_SO4_final_conc
Q5 HotStart High Fidelity DNA Polymerase
PCR.Protocol.amp_polymerase
0
PCR.Protocol.amplicon_length
NA
PCR.Protocol.amplification_target
30
PCR.Protocol.cycle_annealing_duration
static
PCR.Protocol.cycle_annealing_method
50
PCR.Protocol.cycle_annealing_temp
20
PCR.Protocol.cycle_count
10
PCR.Protocol.cycle_denaturation_duration
98
PCR.Protocol.cycle_denaturation_temp
30
PCR.Protocol.cycle_extension_duration
72
PCR.Protocol.cycle_extension_temp
200
PCR.Protocol.dATP_final_conc
200
PCR.Protocol.dCTP_final_conc
200
PCR.Protocol.dGTP_final_conc
200
PCR.Protocol.dTTP_final_conc
0
PCR.Protocol.dUTP_final_conc
30
PCR.Protocol.denaturation_duration_initial
98
PCR.Protocol.denaturation_temp_initial
NA
PCR.Protocol.description
2017-11-06T22:06:22.526Z
PCR.Protocol.dtstamp
600
PCR.Protocol.extension_duration_final
72
PCR.Protocol.extension_temp
30
PCR.Protocol.extension_temp_duration
72
PCR.Protocol.extension_temp_final
0
PCR.Protocol.gelatin_final_conc
553
PCR.Protocol.id
16S_V4_Library_Preparation
PCR.Protocol.name
NA
PCR.Protocol.other_additives
0
PCR.Protocol.pcr_buffer_pH
Agencourt Ampure XP Beads
PCR.Protocol.pcr_clean_up_kits
bead
PCR.Protocol.pcr_clean_up_methods
NEB Q5 HotStart High-Fidelity 2x Master Mix
PCR.Protocol.pcr_kit
25
PCR.Protocol.pcr_volume
NA
PCR.Protocol.pcr_water
0
PCR.Protocol.pmid
0
PCR.Protocol.polymerase_units
NA
PCR.Protocol.primer_fwd_seq
NA
PCR.Protocol.primer_rev_seq
373
PCR.Protocol.protocol_id
Invitrogen Quant-it PicoGreen dsDNA Assay kit
PCR.Protocol.quantification_kit
V4
PCR.Protocol.region
2.5
PCR.Protocol.reverse_primer_final_conc
0
PCR.Protocol.tail_duration
NA
PCR.Protocol.tail_polymerase
0
PCR.Protocol.tail_temp
Veriti Thermocycler (Applied Biosystems)
PCR.Protocol.themocycler
0
PCR.Protocol.triton_X_100_final_conc
1
PCR.Protocol.user_id
False
Pet.bird..Biospecimen.
True
Pet.cat..Biospecimen.
False
Pet.dog..Biospecimen.
True
Pet.fish..Biospecimen.
False
Pet.horse..Biospecimen.
False
Pet.reptile..Biospecimen.
True
Pets.in.home..Biospecimen.
NA
Pica.disease..Biospecimen.
Sometimes
Picks.up.objects.to.show.to.others..Biospecimen.
NA
PicoGreen.kit.lot.number..Extract.
Never
Plays.imaginatively.when.alone..Biospecimen.
Rarely
Plays.imaginatively.with.others..Biospecimen.
Sometimes
Plays.in.a.group.with.others..Biospecimen.
MS0049
Pooled.library.batch.ID..Sequencing...OmicsFileSet.
Never
Probiotic..consumption.frequency...Biospecimen.
False
Problems.collecting.stool.sample..M3...Biospecimen.
C03
Quant.Plate.Location1..Extract.
C04
Quant.Plate.Location2..Extract.
KF028B_A
Quant.Plate.Number..Extract.
Caucasian
Racial.group..Biospecimen.
Several times weekly
Ready.to.eat.meals..consumption.frequency...Biospecimen.
Somewhat elevated anxiety
Recent.anxiety..caretaker.reported...Biospecimen.
True
Recently.ill..Biospecimen.
NA
Red.meat..consumption.frequency...Biospecimen.
Regularly
Repetitive.motion..Biospecimen.
Highly sensitive to typical sounds
Response.to.typical.sounds..Biospecimen.
Rarely
Restaurant.prepared.meals..consumption.frequency...Biospecimen.
SRS15613742
SRA accession
Never
Seafood..consumption.frequency...Biospecimen.
Dangerous or frequent self-harming behavior
Self.injurious.behavior.finding..Biospecimen.
SG Soil Lab Sequencing Protocol
Sequencing.Protocol.description
2017-06-20T22:06:22.526Z
Sequencing.Protocol.dtstamp
2
Sequencing.Protocol.id
Illumina MiSeq
Sequencing.Protocol.instrument_model
7
Sequencing.Protocol.loading_lib_concentration
pmol/L
Sequencing.Protocol.loading_lib_concentration_unit
5134
Sequencing.Protocol.loading_lib_concentration_unit_ontology_id
MiSeq_V4_v1.0
Sequencing.Protocol.name
Bioanalyzer is used to asses quality of pooled library. PhiX control spike-in is 20-15% of final load.
Sequencing.Protocol.notes
359
Sequencing.Protocol.protocol_id
251
Sequencing.Protocol.read_length
MiSeq Reagent Kit v3 (600 cycle)
Sequencing.Protocol.sequencing_kit
4
Sequencing.Protocol.starting_lib_concentration
nmol/L
Sequencing.Protocol.starting_lib_concentration_unit
3135
Sequencing.Protocol.starting_lib_concentration_unit_ontology_id
10
Sequencing.Protocol.user_id
M05416_40_000000000_BTH43
Sequencing.run.ID..OmicsFileSet.
False
Shares.bed.with.pet..Biospecimen.
Constant sleep difficulties
Sleep.pattern.finding..Biospecimen.
False
Small.pet.rodent..Biospecimen.
Drinking water from city
Source.of.drinking.water..Biospecimen.
None
Specific.food.allergy..Biospecimen.
LR
Standard.Curve..Extract.
3-4 meals per week
Starchy.food..consumption.frequency...longitudinal...Biospecimen.
3
Stool.frequency..Biospecimen.
NA
Stool.sample.challenges.explained..M3...Biospecimen.
Sampled from diaper
Stool.sample.collection.method..M3...Biospecimen.
Daily
Sugary.food..consumption.frequency...Biospecimen.
Never
Sweetened.drink..consumption.frequency...Biospecimen.
NA
Toilet.cover..M3...Biospecimen.
False
Toilet.trained..Biospecimen.
NA
Total.Volume.of.Sample..Extract.
NA
Travel.outside.US..notes...Biospecimen.
NA
Travel.to.country..Biospecimen.
Understands most words
Understands.speech..Biospecimen.
Daily
Vegetable..consumption.frequency...Biospecimen.
7-10 meals per week
Vegetable..consumption.frequency...longitudinal...Biospecimen.
Never
Vitamin.B.complex.supplement..consumption.frequency...Biospecimen.
Never
Vitamin.D..consumption.frequency...Biospecimen.
NA
Volume.DNA.to.add..Extract.
NA
Volume.H2O.to.add..Extract.
Weekly
Whole.grain..consumption.frequency...Biospecimen.
Timepoint 3
Within.study.sampling.date..Biospecimen.
70
age
months
age_units
not applicable
biomaterial_provider
not applicable
isolate
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
organism
A
phenotype
female
sex
stool
tissue
Human sample from Homo sapiens
title