SAMN29017487
External Id
University of New South Wales
INSDC center name
2023-06-30T00:54:07.640Z
INSDC first public
2023-06-30T00:54:07.640Z
INSDC last update
SRS13387944
INSDC secondary accession
live
INSDC status
MIMS.me
NCBI submission model
MIGS/MIMS/MIMARKS.soil
NCBI submission model
MIMS.me.soil.6.0
NCBI submission package
SRS13387944
SRA accession
553
annual_season_precpt
11.6 - 19.2
annual_season_temp
2020-06-28
collection_date
dry sclerophyll woodland
cur_vegetation
0-5cm
depth
Keywords: GSC:MIxS;MIMS:6.0
description
230
elev
ENVO:01000208
env_broad_scale
ENVO:01000208
env_local_scale
ENVO:01000208 Soil 6
env_medium
Once burnt between 1960-present, December 2019
fire
Australia: Western River, Kangaroo Island, South Australia
geo_loc_name
soil sample
isolation_source
35.72 S 137.00 E
lat_lon
Ferric Brown-Red Chromosol-Sodosol-Kurosol
local_class
Soil data from NatureMaps were used to identify soil type, and soil classifications were further defined via the Snelling land system report: Department for Environment and Water, 2009a. Soils (soil type) [dataset]. NatureMaps. http://location.sa.gov.au/lms/Reports/ReportMetadata.aspx?pu=y&p_no=100+&pa=dewnr (accessed 21 May 2021). DEWNR Soil and Land Program, n.d. Snelling land system report. https://data.environment.sa.gov.au/Content/Land-System reports/SNE.pdf (accessed 1 January 2022).
local_class_meth
soil metagenome
http://purl.obolibrary.org/obo/NCBITaxon_410658
organism
5.61
ph
Soil diluted in deionized water at a 1:5 ratio using a pH meter, respectively (Instrument Choice, SA, Australia)
ph_meth
clearing and grazing, prior to 2009
previous_land_use
Interview of current landowner
previous_land_use_meth
Soil core
samp_collect_device
Sample was stored on ice and shipped to UNSW Sydney where it was stored at -80oC. Rocks, organic matter, and other debris were removed manually, three soil samples were pooled into one replicate sample, and soil aggregates were crushed.
samp_mat_process
150mL
samp_size
metagenomic assembly
sample_type
None
sieving
45-90
slope_aspect
6.842773413
slope_gradient
Ironstone soil
soil_type
Soil data from NatureMaps were used to identify soil type, and soil types were further defined via the Snelling land system report: Department for Environment and Water, 2009a. Soils (soil type) [dataset]. NatureMaps. http://location.sa.gov.au/lms/Reports/ReportMetadata.aspx?pu=y&p_no=100+&pa=dewnr (accessed 21 May 2021). DEWNR Soil and Land Program, n.d. Snelling land system report. https://data.environment.sa.gov.au/Content/Land-System reports/SNE.pdf (accessed 1 January 2022).
soil_type_meth
Stored at 4oC for 24 days, stored at -80oC for 196 days
store_cond
Burnt-Managed_Site-6_Rep-2
title
vario MACRO cube (Elementar, Sydney, NSW, Australia) at the Mark Wainwright Analytical Centre (UNSW Sydney)
tot_n_meth
0.63
tot_nitro
Total organic carbon (TOC) was measured by potassium dichromate (K2Cr2O7) and sulfuric acid (H2SO4) extraction (Mingorance et al., 2007). Calibration standards of 0-12mg/mL of carbon were made by dissolving 7.1204g of sucrose in 250mL distilled water and diluting into distilled water. 1mL of each standard underwent the following protocol alongside soil samples. For each soil sample, 3mL of 1N K2Cr2O7 and 6mL of 99% H2SO4 were added to 100mg of soil. Samples were loosely capped and gently swirled, left standing for 3 hours, and then 10mL of distilled water was added to each sample. Samples were tightly capped and then shaken on an orbital shaker for 15 minutes at 100rpm, then left standing for 48 hours. Absorbance of sample supernatant was then measured by an Epoch2 Microplate Spectrophotometer (BioTek Instruments, VT, USA) at the 590nm wavelength. Mingorance, M. D., Barahona, E., & Fernndez-Glvez, J. (2007). Guidelines for improving organic carbon recovery by the wet oxidation method. Chemosphere, 68(3), 409413. https://doi.org/10.1016/j.chemosphere.2007.01.037
tot_org_c_meth
8.628742515
tot_org_carb