We recently (6 months ago) figured that he regular cause him reflux issues which we gave him until he was almost 6. He had multiple ear and stomach infections during 0-6. We give him casein free goat/a2 milk now and he seems to be doing better with that.
Additional.info.affecting.microbiome..M3...Biospecimen.
More than 200000
Annual.household.income..Biospecimen.
False
Anti.infective..Biospecimen.
NA
Autism.spectrum.disorder..Biospecimen.
0.5455
Average.conc..Extract.
NA
Batch..OmicsFileSet.
True
Behavior.video.submitted..M3...Biospecimen.
B18012647
Biospecimen.Barcode
2018-03-24T00:00:00
Biospecimen.Date.Collected
Matched with metadata entry:ASD_T3_Family_187
Biospecimen.Description
153278
Biospecimen.ID
M3_187_P3_A_V1
Biospecimen.Name
0.23
Biospecimen.consumption
g
Biospecimen.consumption.unit
SBIR BOX 34_D2
Biospecimen.location..Extract.
NA
Biospecimen.mass
NA
Biospecimen.mass.unit
False
Born.by.caesarian.section..Biospecimen.
False
Born.prematurely..Biospecimen.
3-4 meals per week
Bread..consumption.frequency...longitudinal...Biospecimen.
Developmentally delayed (not clearly autism)
Childhood.behavioral.development.finding..Biospecimen.
0.558
Conc1..Extract.
0.533
Conc2..Extract.
False
Constipation..Biospecimen.
Limited conversation ability
Conversation.ability..Biospecimen.
Weekly
Dairy..consumption.frequency...Biospecimen.
3-4 meals per week
Dairy..consumption.frequency...longitudinal...Biospecimen.
False
Diarrhea..Biospecimen.
Mother's milk diet
Diet.during.infancy..Biospecimen.
7-10 meals per week
Dietary.fat.and.oil..consumption.frequency...longitudinal...Biospecimen.
Vegetarian diet
Dietary.regime..Biospecimen.
True
Dietary.restrictions..M3...Biospecimen.
Tend to eat only limited variety of foods.
Dietary.restrictions.details..M3...Biospecimen.
NA
Dietary.supplement..Biospecimen.
False
Environmental.tobacco.smoke.exposure..Biospecimen.
NA
Explain.how.you.collected.stool.sample..M3...Biospecimen.
SAMN31514914
External Id
Z19014223
Extract.Barcode
2019-03-25T00:00:00
Extract.Date.Collected
199947
Extract.ID
M3_187_P3_A_V1 (Extract)
Extract.Name
109.1
Extract.concentration
ng/microliter
Extract.concentration.unit
5
Extract.consumption
microliter
Extract.consumption.unit
30
Extract.volume
uL
Extract.volume.unit
DNA extraction using KingFisher and Zymo DNA clean up kit
Extraction.Protocol.description
glass
Extraction.Protocol.disruption_bead_type
600
Extraction.Protocol.disruption_duration
20
Extraction.Protocol.disruption_intensity
23
Extraction.Protocol.disruption_temperature
2019-03-29T15:41:15.874Z
Extraction.Protocol.dtstamp
gDNA
Extraction.Protocol.extract_type
Qiagen MagAttract PowerMicrobiome DNA/RNA Kit #27600-4-KF. ZR-96 DNA Clean-up kit Zymo #D4017, D4018
Extraction.Protocol.extraction_kit
H2O
Extraction.Protocol.final_buffer
352
Extraction.Protocol.id
DNA Extraction for the M3 Project
Extraction.Protocol.name
This protocol is developed to accommodate the fact that for M3 project, many of the stool samples in Second Genome preservative were watery. The protocol is modified based on the SOP-SL-100 with additional steps for sample pre-processing and final clean-up.
Extraction.Protocol.notes
30
Extraction.Protocol.nucleic_acid_eluted_volume
553
Extraction.Protocol.protocol_id
KingFisher ClearMag bead-based combined with Zymo column-based clean up
Extraction.Protocol.purification_method
-20
Extraction.Protocol.storage_temperature
65
Extraction.Protocol.user_id
KF0077
Extraction.batch.ID..Extract.
160042804
Extraction.kit.lot.number..Extract.
Consistent eye contact
Eye.contact.finding..Biospecimen.
187
Family.group.ID..Biospecimen.
Weekly
Fermented.vegetable..consumption.frequency...Biospecimen.
False
Fever..Biospecimen.
False
Flu.like.symptoms..Biospecimen.
6 months
Flu.shot.in.the.last..M3...Biospecimen.
NA
Flu.shot.in.the.last..MFlu.shot.in.the.last..M3...Biospecimen.
NA
Foreign.travel.within.past.months..Biospecimen.
NA
Frequency.of.interaction.with.pet.s...Biospecimen.
Rarely
Fruit..consumption.frequency...Biospecimen.
3-4 meals per week
Fruit..consumption.frequency...longitudinal...Biospecimen.
Tends to have constipation
Functional.bowel.finding..Biospecimen.
False
GI.issues.one.month.ago..M3...Biospecimen.
True
GI.issues.this.week..M3...Biospecimen.
False
GI.issues.three.months.ago..M3...Biospecimen.
False
GI.issues.two.months.ago..M3...Biospecimen.
False
GI.issues.two.weeks.ago..M3...Biospecimen.
True
GI.symptoms.within.3.months..M3...Biospecimen.
No issues
Gastrointestinal.problems..M3...Biospecimen.
NA
Gestational.age..Biospecimen.
False
Gluten.allergy..Biospecimen.
12314
Host.ID
187_A
Host.Name
Case (disease status)
Host.disease.status..Biospecimen.
NA
Host.interaction.with.pets..Biospecimen.
Oregon State University
INSDC center name
2023-07-01T00:00:00Z
INSDC first public
2023-07-01T13:44:55.104Z
INSDC last update
SRS15613595
INSDC secondary accession
live
INSDC status
Imitates actions or gestures of others
Imitation.behavior..Biospecimen.
NA
Indoors.pet..Biospecimen.
False
Indoors.pets..Biospecimen.
NA
LR.L2.2.Video.classifier.score..M3...Biospecimen.
True
Lactose.intolerance..Biospecimen.
Able to speak fluently
Language.ability.and.use..Biospecimen.
NA
Length.of.recent.foreign.travel..Biospecimen.
L19016226
Library.Barcode
2019-03-27T00:00:00
Library.Date.Collected
200023
Library.ID
NA
Library.Name
16.3
Library.concentration
ng/uL
Library.concentration.unit
NA
Library.mass
NA
Library.mass.unit
40
Library.volume
uL
Library.volume.unit
C06
Location.on.Extraction.Plate..Extract.
Daily
Meals.prepared.at.home..consumption.frequency...Biospecimen.
Never
Meat..consumption.frequency...Biospecimen.
Never or less than once per week
Meats.and.seafood..consumption.frequency...longitudinal...Biospecimen.
3
Minimum.time.since.antibiotics..Biospecimen.
-6.999
Mobile.Autism.Risk.Assessment.Score..Biospecimen.
Constipation
Most.recent.GI.episode.symptoms..M3...Biospecimen.
False
Multivitamin..Biospecimen.
Human
NCBI submission model
Human.1.0
NCBI submission package
460
Natural.father.current.age..months...Biospecimen.
445
Natural.mother.current.age..months...Biospecimen.
187_N
Natural.sibling.included.in.present.study..Biospecimen.
False
Non.celiac.gluten.sensitivity..Biospecimen.
5
Normalized.concentration..Extract.
0
Number.of.pet.birds..Biospecimen.
0
Number.of.pet.cats..Biospecimen.
0
Number.of.pet.dogs..Biospecimen.
0
Number.of.pet.fish..Biospecimen.
0
Number.of.pet.horses..Biospecimen.
0
Number.of.pet.reptiles..Biospecimen.
0
Number.of.roommates..Biospecimen.
Daily
Olive.oil.used.in.cooking..M3...Biospecimen.
L19016226_M05416_77_000000000-C29M9
OmicsFileSet.Barcode
202416
OmicsFileSet.ID
L19016226_M05416_77_000000000-C29M9
OmicsFileSet.Name
NA
Other.GI.symptoms..M3...Biospecimen.
False
Other.symptoms.this.week..M3...Biospecimen.
NA
Outdoors.pet..Biospecimen.
False
Outdoors.pets..Biospecimen.
NA
PCR.Protocol.BSA_final_conc
NA
PCR.Protocol.KCl_final_conc
NA
PCR.Protocol.MgCl2_final_conc
NA
PCR.Protocol.MgSO4_final_conc
NA
PCR.Protocol.Tris_HCl_final_conc
NA
PCR.Protocol.Tris_SO4_final_conc
Q5 Hot Start High-Fidelity 2X Master Mix
PCR.Protocol.amp_polymerase
NA
PCR.Protocol.amplicon_length
NA
PCR.Protocol.amplification_target
NA
PCR.Protocol.cycle_annealing_duration
NA
PCR.Protocol.cycle_annealing_method
NA
PCR.Protocol.cycle_annealing_temp
20
PCR.Protocol.cycle_count
NA
PCR.Protocol.cycle_denaturation_duration
NA
PCR.Protocol.cycle_denaturation_temp
NA
PCR.Protocol.cycle_extension_duration
NA
PCR.Protocol.cycle_extension_temp
NA
PCR.Protocol.dATP_final_conc
NA
PCR.Protocol.dCTP_final_conc
NA
PCR.Protocol.dGTP_final_conc
NA
PCR.Protocol.dTTP_final_conc
NA
PCR.Protocol.dUTP_final_conc
NA
PCR.Protocol.denaturation_duration_initial
NA
PCR.Protocol.denaturation_temp_initial
NA
PCR.Protocol.description
2018-06-04T17:32:01.019Z
PCR.Protocol.dtstamp
NA
PCR.Protocol.extension_duration_final
NA
PCR.Protocol.extension_temp
NA
PCR.Protocol.extension_temp_duration
NA
PCR.Protocol.extension_temp_final
NA
PCR.Protocol.gelatin_final_conc
575
PCR.Protocol.id
MAD LAB 16S V4 Library Preparation (20 cycles)
PCR.Protocol.name
NA
PCR.Protocol.other_additives
NA
PCR.Protocol.pcr_buffer_pH
AMPure XP
PCR.Protocol.pcr_clean_up_kits
Bead
PCR.Protocol.pcr_clean_up_methods
NA
PCR.Protocol.pcr_kit
50
PCR.Protocol.pcr_volume
NA
PCR.Protocol.pcr_water
NA
PCR.Protocol.pmid
NA
PCR.Protocol.polymerase_units
NA
PCR.Protocol.primer_fwd_seq
NA
PCR.Protocol.primer_rev_seq
463
PCR.Protocol.protocol_id
PicoGreen
PCR.Protocol.quantification_kit
V4
PCR.Protocol.region
NA
PCR.Protocol.reverse_primer_final_conc
NA
PCR.Protocol.tail_duration
NA
PCR.Protocol.tail_polymerase
NA
PCR.Protocol.tail_temp
NA
PCR.Protocol.themocycler
NA
PCR.Protocol.triton_X_100_final_conc
24
PCR.Protocol.user_id
False
Pet.bird..Biospecimen.
False
Pet.cat..Biospecimen.
False
Pet.dog..Biospecimen.
False
Pet.fish..Biospecimen.
False
Pet.horse..Biospecimen.
False
Pet.reptile..Biospecimen.
False
Pets.in.home..Biospecimen.
NA
Pica.disease..Biospecimen.
Regularly
Picks.up.objects.to.show.to.others..Biospecimen.
2047632
PicoGreen.kit.lot.number..Extract.
Sometimes
Plays.imaginatively.when.alone..Biospecimen.
Sometimes
Plays.imaginatively.with.others..Biospecimen.
Sometimes
Plays.in.a.group.with.others..Biospecimen.
NA
Pooled.library.batch.ID..Sequencing...OmicsFileSet.
Occasionally
Probiotic..consumption.frequency...Biospecimen.
False
Problems.collecting.stool.sample..M3...Biospecimen.
C3
Quant.Plate.Location1..Extract.
C4
Quant.Plate.Location2..Extract.
2
Quant.Plate.Number..Extract.
Indian race
Racial.group..Biospecimen.
Rarely
Ready.to.eat.meals..consumption.frequency...Biospecimen.
No elevated anxiety
Recent.anxiety..caretaker.reported...Biospecimen.
False
Recently.ill..Biospecimen.
Never
Red.meat..consumption.frequency...Biospecimen.
Sometimes
Repetitive.motion..Biospecimen.
Highly sensitive to typical sounds
Response.to.typical.sounds..Biospecimen.
Weekly
Restaurant.prepared.meals..consumption.frequency...Biospecimen.
SRS15613595
SRA accession
Never
Seafood..consumption.frequency...Biospecimen.
Mild self-harming behavior
Self.injurious.behavior.finding..Biospecimen.
NA
Sequencing.Protocol.description
NA
Sequencing.Protocol.dtstamp
NA
Sequencing.Protocol.id
NA
Sequencing.Protocol.instrument_model
NA
Sequencing.Protocol.loading_lib_concentration
NA
Sequencing.Protocol.loading_lib_concentration_unit
NA
Sequencing.Protocol.loading_lib_concentration_unit_ontology_id
NA
Sequencing.Protocol.name
NA
Sequencing.Protocol.notes
NA
Sequencing.Protocol.protocol_id
NA
Sequencing.Protocol.read_length
NA
Sequencing.Protocol.sequencing_kit
NA
Sequencing.Protocol.starting_lib_concentration
NA
Sequencing.Protocol.starting_lib_concentration_unit
NA
Sequencing.Protocol.starting_lib_concentration_unit_ontology_id
NA
Sequencing.Protocol.user_id
M05416_77_000000000-C29M9
Sequencing.run.ID..OmicsFileSet.
False
Shares.bed.with.pet..Biospecimen.
Some sleep difficulties
Sleep.pattern.finding..Biospecimen.
False
Small.pet.rodent..Biospecimen.
Drinking water is filtered
Source.of.drinking.water..Biospecimen.
None
Specific.food.allergy..Biospecimen.
HR
Standard.Curve..Extract.
3-4 meals per week
Starchy.food..consumption.frequency...longitudinal...Biospecimen.
Less than 1
Stool.frequency..Biospecimen.
NA
Stool.sample.challenges.explained..M3...Biospecimen.
NA
Stool.sample.collection.method..M3...Biospecimen.
Weekly
Sugary.food..consumption.frequency...Biospecimen.
Never
Sweetened.drink..consumption.frequency...Biospecimen.
Yes, I used the toilet cover when collecting samples from both of my children
Toilet.cover..M3...Biospecimen.
True
Toilet.trained..Biospecimen.
30
Total.Volume.of.Sample..Extract.
NA
Travel.outside.US..notes...Biospecimen.
NA
Travel.to.country..Biospecimen.
Understands many words
Understands.speech..Biospecimen.
Weekly
Vegetable..consumption.frequency...Biospecimen.
3-4 meals per week
Vegetable..consumption.frequency...longitudinal...Biospecimen.
Occasionally
Vitamin.B.complex.supplement..consumption.frequency...Biospecimen.
Occasionally
Vitamin.D..consumption.frequency...Biospecimen.
1.374885426
Volume.DNA.to.add..Extract.
28.62511457
Volume.H2O.to.add..Extract.
Daily
Whole.grain..consumption.frequency...Biospecimen.
Timepoint 3
Within.study.sampling.date..Biospecimen.
79
age
months
age_units
not applicable
biomaterial_provider
not applicable
isolate
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
organism
A
phenotype
male
sex
stool
tissue
Human sample from Homo sapiens
title