SAMN29473155
External Id
Stowers Institute for Medical Research
INSDC center name
2023-07-01T00:50:40.413Z
INSDC first public
2023-07-01T00:50:40.413Z
INSDC last update
SRS13653730
INSDC secondary accession
live
INSDC status
IP
IP_input
Model organism or animal
NCBI submission model
Model.organism.animal.1.0
NCBI submission package
SRS13653730
SRA accession
2-16h
age
Canton
breed
Chn WT ChIPseq replicate 6
description
WT
genotype
Fly stocks were maintained with standard protocols. Embryos were collected from food plates (corn+yeast extract+sugar+agar) at 25C and used directly for experiments.
growth_protocol
Drosophila melanogaster
http://purl.obolibrary.org/obo/NCBITaxon_7227
organism
1
replicate
pooled male and female
sex
whole embryo
tissue
Chn WT ChIPseq replicate 6
title
Embryos were cross linked in 1.8% formaldehyde, chromatin was sonicated to an average size of ∼500 bp (whole cell extract (WCE)), and 300 μl WCE from ∼120 mg embryos was incubated with protein A–conjugated Dynabeads (Invitrogen 100-02D), coated with antibodies against D. melanogaster Chn, Med-19 (Custom antibodies raised the lab) and histones marks (H3K27Ac, H3K3me1 and H3K4me3). For input controls, 50 μl WCE was used. Preparations of DNA libraries for single-end sequencing were done according to instructions from Illumina.
treatment