SAMN29620766
External Id
IGMM- CNRS
INSDC center name
2023-07-01T00:50:41.737Z
INSDC first public
2023-07-01T00:50:41.737Z
INSDC last update
SRS13771166
INSDC secondary accession
live
INSDC status
MIGS.eu
NCBI submission model
MIGS.eu.6.0
NCBI submission package
SRS13771166
SRA accession
not applicable
collection_date
not applicable
cultivar
In vivo footprinting of Sir3 from a time course after exit from starvation uisng Sir3Dam and direct detection of GATC methylation by nanopore sequencing (Oxford Nanopore). Fast5 files basecalled with guppy basecaller and dna_r9.4.1_450bps_modbases_dam-dcm-cpg_hac.cfg. Multiplexed library mix with the Rapid Barcoding Kit (RBK409: Sir3Dam in G1 arrsted by alpha factor; RBK407: 30min after release from starvation; RBK408: 90min after release from starvation; RBK411: Rpc82Dam from a saturated culture).
description
not applicable
ecotype
not applicable
env_broad_scale
not applicable
env_local_scale
not applicable
env_medium
not applicable
geo_loc_name
not applicable
isol_growth_condt
30 and 90min after exit from starvation (glucose depletion)
isolate
not applicable
lat_lon
Saccharomyces cerevisiae
http://purl.obolibrary.org/obo/NCBITaxon_4932
organism
Sir3Dam (MATa ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 SIR3:: Myc9-DAM (methylase from E.coli) Phleomycin)
strain
MIGS Eukaryotic sample from Saccharomyces cerevisiae
title