He receives a fiber supplement daily
Additional.info.affecting.microbiome..M3...Biospecimen.
80001-150001
Annual.household.income..Biospecimen.
False
Anti.infective..Biospecimen.
NA
Autism.spectrum.disorder..Biospecimen.
31.858
Average.conc..Extract.
batch5re
Batch..OmicsFileSet.
True
Behavior.video.submitted..M3...Biospecimen.
B18012079
Biospecimen.Barcode
2018-02-09T00:00:00
Biospecimen.Date.Collected
Matched with metadata entry ASD_T2_Family_035
Biospecimen.Description
152353
Biospecimen.ID
M3_035_P2_A_V1
Biospecimen.Name
0.25
Biospecimen.consumption
gram
Biospecimen.consumption.unit
NA
Biospecimen.location..Extract.
NA
Biospecimen.mass
NA
Biospecimen.mass.unit
False
Born.by.caesarian.section..Biospecimen.
False
Born.prematurely..Biospecimen.
Almost every meal
Bread..consumption.frequency...longitudinal...Biospecimen.
Developmentally delayed (not clearly autism)
Childhood.behavioral.development.finding..Biospecimen.
32.61
Conc1..Extract.
31.106
Conc2..Extract.
False
Constipation..Biospecimen.
Limited conversation ability
Conversation.ability..Biospecimen.
Daily
Dairy..consumption.frequency...Biospecimen.
7-10 meals per week
Dairy..consumption.frequency...longitudinal...Biospecimen.
False
Diarrhea..Biospecimen.
Mother's milk diet
Diet.during.infancy..Biospecimen.
7-10 meals per week
Dietary.fat.and.oil..consumption.frequency...longitudinal...Biospecimen.
Omnivorous diet
Dietary.regime..Biospecimen.
False
Dietary.restrictions..M3...Biospecimen.
NA
Dietary.restrictions.details..M3...Biospecimen.
False
Dietary.supplement..Biospecimen.
False
Environmental.tobacco.smoke.exposure..Biospecimen.
NA
Explain.how.you.collected.stool.sample..M3...Biospecimen.
SAMN31514805
External Id
Z18010494
Extract.Barcode
NA
Extract.Date.Collected
156087
Extract.ID
NA
Extract.Name
6.3716
Extract.concentration
ng/microliter
Extract.concentration.unit
NA
Extract.consumption
NA
Extract.consumption.unit
100
Extract.volume
uL
Extract.volume.unit
NA
Extraction.Protocol.description
Glass
Extraction.Protocol.disruption_bead_type
600
Extraction.Protocol.disruption_duration
1750
Extraction.Protocol.disruption_intensity
NA
Extraction.Protocol.disruption_temperature
2018-05-10T18:01:55.521Z
Extraction.Protocol.dtstamp
gDNA
Extraction.Protocol.extract_type
MagAttract PowerMicrobiome DNA/RNA KF
Extraction.Protocol.extraction_kit
RNAse-Free Water
Extraction.Protocol.final_buffer
316
Extraction.Protocol.id
MAD LAB High Throughput Extraction of DNA using the MagAttract PowerMicrobiome DNA/RNA KF Kit
Extraction.Protocol.name
NA
Extraction.Protocol.notes
100
Extraction.Protocol.nucleic_acid_eluted_volume
452
Extraction.Protocol.protocol_id
Magnetic Beads
Extraction.Protocol.purification_method
-20
Extraction.Protocol.storage_temperature
24
Extraction.Protocol.user_id
KF027_B
Extraction.batch.ID..Extract.
NA
Extraction.kit.lot.number..Extract.
Some eye contact
Eye.contact.finding..Biospecimen.
35
Family.group.ID..Biospecimen.
Never
Fermented.vegetable..consumption.frequency...Biospecimen.
False
Fever..Biospecimen.
False
Flu.like.symptoms..Biospecimen.
NA
Flu.shot.in.the.last..M3...Biospecimen.
6 months):Month
Flu.shot.in.the.last..MFlu.shot.in.the.last..M3...Biospecimen.
NA
Foreign.travel.within.past.months..Biospecimen.
Daily
Frequency.of.interaction.with.pet.s...Biospecimen.
Never
Fruit..consumption.frequency...Biospecimen.
3-4 meals per week
Fruit..consumption.frequency...longitudinal...Biospecimen.
Tends to have constipation
Functional.bowel.finding..Biospecimen.
False
GI.issues.one.month.ago..M3...Biospecimen.
True
GI.issues.this.week..M3...Biospecimen.
False
GI.issues.three.months.ago..M3...Biospecimen.
False
GI.issues.two.months.ago..M3...Biospecimen.
False
GI.issues.two.weeks.ago..M3...Biospecimen.
True
GI.symptoms.within.3.months..M3...Biospecimen.
Continuous
Gastrointestinal.problems..M3...Biospecimen.
NA
Gestational.age..Biospecimen.
False
Gluten.allergy..Biospecimen.
9386
Host.ID
035_A
Host.Name
Case (disease status)
Host.disease.status..Biospecimen.
True
Host.interaction.with.pets..Biospecimen.
Oregon State University
INSDC center name
2023-07-01T00:00:00Z
INSDC first public
2023-07-01T13:42:13.950Z
INSDC last update
SRS15613396
INSDC secondary accession
live
INSDC status
Imitates actions or gestures of others
Imitation.behavior..Biospecimen.
True
Indoors.pet..Biospecimen.
NA
Indoors.pets..Biospecimen.
NA
LR.L2.2.Video.classifier.score..M3...Biospecimen.
False
Lactose.intolerance..Biospecimen.
Able to speak fluently
Language.ability.and.use..Biospecimen.
NA
Length.of.recent.foreign.travel..Biospecimen.
L18007963
Library.Barcode
NA
Library.Date.Collected
156171
Library.ID
NA
Library.Name
4.65
Library.concentration
ng/uL
Library.concentration.unit
NA
Library.mass
NA
Library.mass.unit
50
Library.volume
microliter
Library.volume.unit
C11
Location.on.Extraction.Plate..Extract.
Several times weekly
Meals.prepared.at.home..consumption.frequency...Biospecimen.
Weekly
Meat..consumption.frequency...Biospecimen.
Almost every meal
Meats.and.seafood..consumption.frequency...longitudinal...Biospecimen.
3
Minimum.time.since.antibiotics..Biospecimen.
-8.846
Mobile.Autism.Risk.Assessment.Score..Biospecimen.
Constipation & Abdominal bloating
Most.recent.GI.episode.symptoms..M3...Biospecimen.
True
Multivitamin..Biospecimen.
Human
NCBI submission model
Human.1.0
NCBI submission package
456
Natural.father.current.age..months...Biospecimen.
466
Natural.mother.current.age..months...Biospecimen.
035_N
Natural.sibling.included.in.present.study..Biospecimen.
False
Non.celiac.gluten.sensitivity..Biospecimen.
NA
Normalized.concentration..Extract.
0
Number.of.pet.birds..Biospecimen.
0
Number.of.pet.cats..Biospecimen.
1
Number.of.pet.dogs..Biospecimen.
0
Number.of.pet.fish..Biospecimen.
0
Number.of.pet.horses..Biospecimen.
0
Number.of.pet.reptiles..Biospecimen.
0
Number.of.roommates..Biospecimen.
Several times weekly
Olive.oil.used.in.cooking..M3...Biospecimen.
L18007963_M05416_45_000000000_BV4GK
OmicsFileSet.Barcode
157945
OmicsFileSet.ID
_O1
OmicsFileSet.Name
False
Other.GI.symptoms..M3...Biospecimen.
False
Other.symptoms.this.week..M3...Biospecimen.
False
Outdoors.pet..Biospecimen.
NA
Outdoors.pets..Biospecimen.
0
PCR.Protocol.BSA_final_conc
0
PCR.Protocol.KCl_final_conc
0
PCR.Protocol.MgCl2_final_conc
0
PCR.Protocol.MgSO4_final_conc
0
PCR.Protocol.Tris_HCl_final_conc
0
PCR.Protocol.Tris_SO4_final_conc
Q5 HotStart High Fidelity DNA Polymerase
PCR.Protocol.amp_polymerase
0
PCR.Protocol.amplicon_length
NA
PCR.Protocol.amplification_target
30
PCR.Protocol.cycle_annealing_duration
static
PCR.Protocol.cycle_annealing_method
50
PCR.Protocol.cycle_annealing_temp
20
PCR.Protocol.cycle_count
10
PCR.Protocol.cycle_denaturation_duration
98
PCR.Protocol.cycle_denaturation_temp
30
PCR.Protocol.cycle_extension_duration
72
PCR.Protocol.cycle_extension_temp
200
PCR.Protocol.dATP_final_conc
200
PCR.Protocol.dCTP_final_conc
200
PCR.Protocol.dGTP_final_conc
200
PCR.Protocol.dTTP_final_conc
0
PCR.Protocol.dUTP_final_conc
30
PCR.Protocol.denaturation_duration_initial
98
PCR.Protocol.denaturation_temp_initial
NA
PCR.Protocol.description
2017-11-06T22:06:22.526Z
PCR.Protocol.dtstamp
600
PCR.Protocol.extension_duration_final
72
PCR.Protocol.extension_temp
30
PCR.Protocol.extension_temp_duration
72
PCR.Protocol.extension_temp_final
0
PCR.Protocol.gelatin_final_conc
553
PCR.Protocol.id
16S_V4_Library_Preparation
PCR.Protocol.name
NA
PCR.Protocol.other_additives
0
PCR.Protocol.pcr_buffer_pH
Agencourt Ampure XP Beads
PCR.Protocol.pcr_clean_up_kits
bead
PCR.Protocol.pcr_clean_up_methods
NEB Q5 HotStart High-Fidelity 2x Master Mix
PCR.Protocol.pcr_kit
25
PCR.Protocol.pcr_volume
NA
PCR.Protocol.pcr_water
0
PCR.Protocol.pmid
0
PCR.Protocol.polymerase_units
NA
PCR.Protocol.primer_fwd_seq
NA
PCR.Protocol.primer_rev_seq
373
PCR.Protocol.protocol_id
Invitrogen Quant-it PicoGreen dsDNA Assay kit
PCR.Protocol.quantification_kit
V4
PCR.Protocol.region
2.5
PCR.Protocol.reverse_primer_final_conc
0
PCR.Protocol.tail_duration
NA
PCR.Protocol.tail_polymerase
0
PCR.Protocol.tail_temp
Veriti Thermocycler (Applied Biosystems)
PCR.Protocol.themocycler
0
PCR.Protocol.triton_X_100_final_conc
1
PCR.Protocol.user_id
False
Pet.bird..Biospecimen.
False
Pet.cat..Biospecimen.
True
Pet.dog..Biospecimen.
False
Pet.fish..Biospecimen.
False
Pet.horse..Biospecimen.
False
Pet.reptile..Biospecimen.
True
Pets.in.home..Biospecimen.
NA
Pica.disease..Biospecimen.
Sometimes
Picks.up.objects.to.show.to.others..Biospecimen.
NA
PicoGreen.kit.lot.number..Extract.
Sometimes
Plays.imaginatively.when.alone..Biospecimen.
Sometimes
Plays.imaginatively.with.others..Biospecimen.
Sometimes
Plays.in.a.group.with.others..Biospecimen.
MS0048c
Pooled.library.batch.ID..Sequencing...OmicsFileSet.
Weekly
Probiotic..consumption.frequency...Biospecimen.
False
Problems.collecting.stool.sample..M3...Biospecimen.
NA
Quant.Plate.Location1..Extract.
NA
Quant.Plate.Location2..Extract.
NA
Quant.Plate.Number..Extract.
Caucasian
Racial.group..Biospecimen.
Several times weekly
Ready.to.eat.meals..consumption.frequency...Biospecimen.
Somewhat elevated anxiety
Recent.anxiety..caretaker.reported...Biospecimen.
False
Recently.ill..Biospecimen.
NA
Red.meat..consumption.frequency...Biospecimen.
Never
Repetitive.motion..Biospecimen.
Sensitive to typical sounds
Response.to.typical.sounds..Biospecimen.
Weekly
Restaurant.prepared.meals..consumption.frequency...Biospecimen.
SRS15613396
SRA accession
Weekly
Seafood..consumption.frequency...Biospecimen.
Mild self-harming behavior
Self.injurious.behavior.finding..Biospecimen.
SG Soil Lab Sequencing Protocol
Sequencing.Protocol.description
2017-06-20T22:06:22.526Z
Sequencing.Protocol.dtstamp
2
Sequencing.Protocol.id
Illumina MiSeq
Sequencing.Protocol.instrument_model
7
Sequencing.Protocol.loading_lib_concentration
pmol/L
Sequencing.Protocol.loading_lib_concentration_unit
5134
Sequencing.Protocol.loading_lib_concentration_unit_ontology_id
MiSeq_V4_v1.0
Sequencing.Protocol.name
Bioanalyzer is used to asses quality of pooled library. PhiX control spike-in is 20-15% of final load.
Sequencing.Protocol.notes
359
Sequencing.Protocol.protocol_id
251
Sequencing.Protocol.read_length
MiSeq Reagent Kit v3 (600 cycle)
Sequencing.Protocol.sequencing_kit
4
Sequencing.Protocol.starting_lib_concentration
nmol/L
Sequencing.Protocol.starting_lib_concentration_unit
3135
Sequencing.Protocol.starting_lib_concentration_unit_ontology_id
10
Sequencing.Protocol.user_id
M05416_45_000000000_BV4GK
Sequencing.run.ID..OmicsFileSet.
False
Shares.bed.with.pet..Biospecimen.
Healthy sleep pattern
Sleep.pattern.finding..Biospecimen.
False
Small.pet.rodent..Biospecimen.
Drinking water from city & Drinking water is filtered
Source.of.drinking.water..Biospecimen.
None
Specific.food.allergy..Biospecimen.
LR
Standard.Curve..Extract.
Almost every meal
Starchy.food..consumption.frequency...longitudinal...Biospecimen.
3
Stool.frequency..Biospecimen.
NA
Stool.sample.challenges.explained..M3...Biospecimen.
Sampled from diaper
Stool.sample.collection.method..M3...Biospecimen.
Several times weekly
Sugary.food..consumption.frequency...Biospecimen.
Never
Sweetened.drink..consumption.frequency...Biospecimen.
NA
Toilet.cover..M3...Biospecimen.
False
Toilet.trained..Biospecimen.
NA
Total.Volume.of.Sample..Extract.
NA
Travel.outside.US..notes...Biospecimen.
NA
Travel.to.country..Biospecimen.
Understands most words
Understands.speech..Biospecimen.
Several times weekly
Vegetable..consumption.frequency...Biospecimen.
3-4 meals per week
Vegetable..consumption.frequency...longitudinal...Biospecimen.
Daily
Vitamin.B.complex.supplement..consumption.frequency...Biospecimen.
Daily
Vitamin.D..consumption.frequency...Biospecimen.
NA
Volume.DNA.to.add..Extract.
NA
Volume.H2O.to.add..Extract.
Weekly
Whole.grain..consumption.frequency...Biospecimen.
Timepoint 2
Within.study.sampling.date..Biospecimen.
62
age
months
age_units
not applicable
biomaterial_provider
not applicable
isolate
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
organism
A
phenotype
male
sex
stool
tissue
Human sample from Homo sapiens
title