NA
Additional.info.affecting.microbiome..M3...Biospecimen.
80001-150001
Annual.household.income..Biospecimen.
True
Anti.infective..Biospecimen.
NA
Autism.spectrum.disorder..Biospecimen.
0.145
Average.conc..Extract.
batch2re
Batch..OmicsFileSet.
True
Behavior.video.submitted..M3...Biospecimen.
B18012171
Biospecimen.Barcode
2017-12-19T00:00:00
Biospecimen.Date.Collected
Matched with metadata entry ASD_T1_Family_053
Biospecimen.Description
152510
Biospecimen.ID
M3_053_P1_A_V1
Biospecimen.Name
0.13
Biospecimen.consumption
gram
Biospecimen.consumption.unit
SBIR Box 2_A3, A4
Biospecimen.location..Extract.
NA
Biospecimen.mass
NA
Biospecimen.mass.unit
False
Born.by.caesarian.section..Biospecimen.
True
Born.prematurely..Biospecimen.
7-10 meals per week
Bread..consumption.frequency...longitudinal...Biospecimen.
Some developmental delays
Childhood.behavioral.development.finding..Biospecimen.
0.152
Conc1..Extract.
0.138
Conc2..Extract.
False
Constipation..Biospecimen.
Cannot have a conversation
Conversation.ability..Biospecimen.
Daily
Dairy..consumption.frequency...Biospecimen.
7-10 meals per week
Dairy..consumption.frequency...longitudinal...Biospecimen.
False
Diarrhea..Biospecimen.
Mixed infant formula and mother's milk diet
Diet.during.infancy..Biospecimen.
7-10 meals per week
Dietary.fat.and.oil..consumption.frequency...longitudinal...Biospecimen.
Omnivorous diet
Dietary.regime..Biospecimen.
False
Dietary.restrictions..M3...Biospecimen.
NA
Dietary.restrictions.details..M3...Biospecimen.
True
Dietary.supplement..Biospecimen.
False
Environmental.tobacco.smoke.exposure..Biospecimen.
NA
Explain.how.you.collected.stool.sample..M3...Biospecimen.
SAMN31514466
External Id
Z18008183
Extract.Barcode
2018-03-09T00:00:00
Extract.Date.Collected
153527
Extract.ID
NA
Extract.Name
29
Extract.concentration
ng/uL
Extract.concentration.unit
NA
Extract.consumption
NA
Extract.consumption.unit
100
Extract.volume
uL
Extract.volume.unit
NA
Extraction.Protocol.description
Glass
Extraction.Protocol.disruption_bead_type
600
Extraction.Protocol.disruption_duration
1750
Extraction.Protocol.disruption_intensity
NA
Extraction.Protocol.disruption_temperature
2018-05-10T18:01:55.521Z
Extraction.Protocol.dtstamp
gDNA
Extraction.Protocol.extract_type
MagAttract PowerMicrobiome DNA/RNA KF
Extraction.Protocol.extraction_kit
RNAse-Free Water
Extraction.Protocol.final_buffer
316
Extraction.Protocol.id
MAD LAB High Throughput Extraction of DNA using the MagAttract PowerMicrobiome DNA/RNA KF Kit
Extraction.Protocol.name
NA
Extraction.Protocol.notes
100
Extraction.Protocol.nucleic_acid_eluted_volume
452
Extraction.Protocol.protocol_id
Magnetic Beads
Extraction.Protocol.purification_method
-20
Extraction.Protocol.storage_temperature
24
Extraction.Protocol.user_id
KF0024
Extraction.batch.ID..Extract.
157026602
Extraction.kit.lot.number..Extract.
Some eye contact
Eye.contact.finding..Biospecimen.
53
Family.group.ID..Biospecimen.
Never
Fermented.vegetable..consumption.frequency...Biospecimen.
False
Fever..Biospecimen.
False
Flu.like.symptoms..Biospecimen.
NA
Flu.shot.in.the.last..M3...Biospecimen.
6 months):Month
Flu.shot.in.the.last..MFlu.shot.in.the.last..M3...Biospecimen.
NA
Foreign.travel.within.past.months..Biospecimen.
Daily
Frequency.of.interaction.with.pet.s...Biospecimen.
Weekly
Fruit..consumption.frequency...Biospecimen.
Never or less than once per week
Fruit..consumption.frequency...longitudinal...Biospecimen.
Tends to have normal bowel function
Functional.bowel.finding..Biospecimen.
False
GI.issues.one.month.ago..M3...Biospecimen.
False
GI.issues.this.week..M3...Biospecimen.
False
GI.issues.three.months.ago..M3...Biospecimen.
False
GI.issues.two.months.ago..M3...Biospecimen.
False
GI.issues.two.weeks.ago..M3...Biospecimen.
False
GI.symptoms.within.3.months..M3...Biospecimen.
No issues
Gastrointestinal.problems..M3...Biospecimen.
36
Gestational.age..Biospecimen.
False
Gluten.allergy..Biospecimen.
9404
Host.ID
053_A
Host.Name
Case (disease status)
Host.disease.status..Biospecimen.
True
Host.interaction.with.pets..Biospecimen.
Oregon State University
INSDC center name
2023-07-01T00:00:00Z
INSDC first public
2023-07-01T13:42:37.154Z
INSDC last update
SRS15613703
INSDC secondary accession
live
INSDC status
Imitates actions or gestures of others
Imitation.behavior..Biospecimen.
True
Indoors.pet..Biospecimen.
NA
Indoors.pets..Biospecimen.
NA
LR.L2.2.Video.classifier.score..M3...Biospecimen.
False
Lactose.intolerance..Biospecimen.
Single word speech
Language.ability.and.use..Biospecimen.
NA
Length.of.recent.foreign.travel..Biospecimen.
L18006356
Library.Barcode
NA
Library.Date.Collected
155160
Library.ID
NA
Library.Name
27.8
Library.concentration
ng/uL
Library.concentration.unit
NA
Library.mass
NA
Library.mass.unit
50
Library.volume
microliter
Library.volume.unit
D09
Location.on.Extraction.Plate..Extract.
Daily
Meals.prepared.at.home..consumption.frequency...Biospecimen.
Weekly
Meat..consumption.frequency...Biospecimen.
Almost every meal
Meats.and.seafood..consumption.frequency...longitudinal...Biospecimen.
1
Minimum.time.since.antibiotics..Biospecimen.
-4.715
Mobile.Autism.Risk.Assessment.Score..Biospecimen.
NA
Most.recent.GI.episode.symptoms..M3...Biospecimen.
False
Multivitamin..Biospecimen.
Human
NCBI submission model
Human.1.0
NCBI submission package
358
Natural.father.current.age..months...Biospecimen.
343
Natural.mother.current.age..months...Biospecimen.
053_N
Natural.sibling.included.in.present.study..Biospecimen.
False
Non.celiac.gluten.sensitivity..Biospecimen.
5
Normalized.concentration..Extract.
0
Number.of.pet.birds..Biospecimen.
0
Number.of.pet.cats..Biospecimen.
2
Number.of.pet.dogs..Biospecimen.
0
Number.of.pet.fish..Biospecimen.
0
Number.of.pet.horses..Biospecimen.
0
Number.of.pet.reptiles..Biospecimen.
0
Number.of.roommates..Biospecimen.
Several times weekly
Olive.oil.used.in.cooking..M3...Biospecimen.
L18006356_M02273_16_000000000_BPR6H
OmicsFileSet.Barcode
156938
OmicsFileSet.ID
_O1
OmicsFileSet.Name
False
Other.GI.symptoms..M3...Biospecimen.
False
Other.symptoms.this.week..M3...Biospecimen.
True
Outdoors.pet..Biospecimen.
NA
Outdoors.pets..Biospecimen.
0
PCR.Protocol.BSA_final_conc
0
PCR.Protocol.KCl_final_conc
0
PCR.Protocol.MgCl2_final_conc
0
PCR.Protocol.MgSO4_final_conc
0
PCR.Protocol.Tris_HCl_final_conc
0
PCR.Protocol.Tris_SO4_final_conc
Q5 HotStart High Fidelity DNA Polymerase
PCR.Protocol.amp_polymerase
0
PCR.Protocol.amplicon_length
NA
PCR.Protocol.amplification_target
30
PCR.Protocol.cycle_annealing_duration
static
PCR.Protocol.cycle_annealing_method
50
PCR.Protocol.cycle_annealing_temp
20
PCR.Protocol.cycle_count
10
PCR.Protocol.cycle_denaturation_duration
98
PCR.Protocol.cycle_denaturation_temp
30
PCR.Protocol.cycle_extension_duration
72
PCR.Protocol.cycle_extension_temp
200
PCR.Protocol.dATP_final_conc
200
PCR.Protocol.dCTP_final_conc
200
PCR.Protocol.dGTP_final_conc
200
PCR.Protocol.dTTP_final_conc
0
PCR.Protocol.dUTP_final_conc
30
PCR.Protocol.denaturation_duration_initial
98
PCR.Protocol.denaturation_temp_initial
NA
PCR.Protocol.description
2017-11-06T22:06:22.526Z
PCR.Protocol.dtstamp
600
PCR.Protocol.extension_duration_final
72
PCR.Protocol.extension_temp
30
PCR.Protocol.extension_temp_duration
72
PCR.Protocol.extension_temp_final
0
PCR.Protocol.gelatin_final_conc
553
PCR.Protocol.id
16S_V4_Library_Preparation
PCR.Protocol.name
NA
PCR.Protocol.other_additives
0
PCR.Protocol.pcr_buffer_pH
Agencourt Ampure XP Beads
PCR.Protocol.pcr_clean_up_kits
bead
PCR.Protocol.pcr_clean_up_methods
NEB Q5 HotStart High-Fidelity 2x Master Mix
PCR.Protocol.pcr_kit
25
PCR.Protocol.pcr_volume
NA
PCR.Protocol.pcr_water
0
PCR.Protocol.pmid
0
PCR.Protocol.polymerase_units
NA
PCR.Protocol.primer_fwd_seq
NA
PCR.Protocol.primer_rev_seq
373
PCR.Protocol.protocol_id
Invitrogen Quant-it PicoGreen dsDNA Assay kit
PCR.Protocol.quantification_kit
V4
PCR.Protocol.region
2.5
PCR.Protocol.reverse_primer_final_conc
0
PCR.Protocol.tail_duration
NA
PCR.Protocol.tail_polymerase
0
PCR.Protocol.tail_temp
Veriti Thermocycler (Applied Biosystems)
PCR.Protocol.themocycler
0
PCR.Protocol.triton_X_100_final_conc
1
PCR.Protocol.user_id
False
Pet.bird..Biospecimen.
False
Pet.cat..Biospecimen.
True
Pet.dog..Biospecimen.
False
Pet.fish..Biospecimen.
False
Pet.horse..Biospecimen.
False
Pet.reptile..Biospecimen.
True
Pets.in.home..Biospecimen.
NA
Pica.disease..Biospecimen.
Sometimes
Picks.up.objects.to.show.to.others..Biospecimen.
1911234
PicoGreen.kit.lot.number..Extract.
Rarely
Plays.imaginatively.when.alone..Biospecimen.
Rarely
Plays.imaginatively.with.others..Biospecimen.
Never
Plays.in.a.group.with.others..Biospecimen.
MS0034
Pooled.library.batch.ID..Sequencing...OmicsFileSet.
Weekly
Probiotic..consumption.frequency...Biospecimen.
False
Problems.collecting.stool.sample..M3...Biospecimen.
D09
Quant.Plate.Location1..Extract.
D10
Quant.Plate.Location2..Extract.
KF0024_1
Quant.Plate.Number..Extract.
Caucasian
Racial.group..Biospecimen.
Never
Ready.to.eat.meals..consumption.frequency...Biospecimen.
No elevated anxiety
Recent.anxiety..caretaker.reported...Biospecimen.
False
Recently.ill..Biospecimen.
NA
Red.meat..consumption.frequency...Biospecimen.
Never
Repetitive.motion..Biospecimen.
Sensitive to typical sounds
Response.to.typical.sounds..Biospecimen.
Rarely
Restaurant.prepared.meals..consumption.frequency...Biospecimen.
SRS15613703
SRA accession
Never
Seafood..consumption.frequency...Biospecimen.
No self-injurious behavior
Self.injurious.behavior.finding..Biospecimen.
SG Soil Lab Sequencing Protocol
Sequencing.Protocol.description
2017-06-20T22:06:22.526Z
Sequencing.Protocol.dtstamp
2
Sequencing.Protocol.id
Illumina MiSeq
Sequencing.Protocol.instrument_model
7
Sequencing.Protocol.loading_lib_concentration
pmol/L
Sequencing.Protocol.loading_lib_concentration_unit
5134
Sequencing.Protocol.loading_lib_concentration_unit_ontology_id
MiSeq_V4_v1.0
Sequencing.Protocol.name
Bioanalyzer is used to asses quality of pooled library. PhiX control spike-in is 20-15% of final load.
Sequencing.Protocol.notes
359
Sequencing.Protocol.protocol_id
251
Sequencing.Protocol.read_length
MiSeq Reagent Kit v3 (600 cycle)
Sequencing.Protocol.sequencing_kit
4
Sequencing.Protocol.starting_lib_concentration
nmol/L
Sequencing.Protocol.starting_lib_concentration_unit
3135
Sequencing.Protocol.starting_lib_concentration_unit_ontology_id
10
Sequencing.Protocol.user_id
M02273_16_000000000_BPR6H
Sequencing.run.ID..OmicsFileSet.
False
Shares.bed.with.pet..Biospecimen.
Some sleep difficulties
Sleep.pattern.finding..Biospecimen.
False
Small.pet.rodent..Biospecimen.
Drinking water from city
Source.of.drinking.water..Biospecimen.
None
Specific.food.allergy..Biospecimen.
HR
Standard.Curve..Extract.
7-10 meals per week
Starchy.food..consumption.frequency...longitudinal...Biospecimen.
1
Stool.frequency..Biospecimen.
NA
Stool.sample.challenges.explained..M3...Biospecimen.
Sampled from diaper
Stool.sample.collection.method..M3...Biospecimen.
Rarely
Sugary.food..consumption.frequency...Biospecimen.
Never
Sweetened.drink..consumption.frequency...Biospecimen.
NA
Toilet.cover..M3...Biospecimen.
False
Toilet.trained..Biospecimen.
100
Total.Volume.of.Sample..Extract.
NA
Travel.outside.US..notes...Biospecimen.
NA
Travel.to.country..Biospecimen.
Understands about half of words
Understands.speech..Biospecimen.
Rarely
Vegetable..consumption.frequency...Biospecimen.
Never or less than once per week
Vegetable..consumption.frequency...longitudinal...Biospecimen.
Never
Vitamin.B.complex.supplement..consumption.frequency...Biospecimen.
Never
Vitamin.D..consumption.frequency...Biospecimen.
17.24137931
Volume.DNA.to.add..Extract.
82.75862069
Volume.H2O.to.add..Extract.
Daily
Whole.grain..consumption.frequency...Biospecimen.
Timepoint 1
Within.study.sampling.date..Biospecimen.
26
age
months
age_units
not applicable
biomaterial_provider
not applicable
isolate
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
organism
A
phenotype
male
sex
stool
tissue
Human sample from Homo sapiens
title