SAMN36013623
External Id
University of Waterloo
INSDC center name
2023-06-28T00:00:00Z
INSDC first public
2023-06-28T08:23:28.167Z
INSDC last update
SRS18087168
INSDC secondary accession
live
INSDC status
Metagenome or environmental
NCBI submission model
Metagenome.environmental.1.0
NCBI submission package
SRS18087168
SRA accession
2017-09
collection_date
This non-axenic bacterial enrichment culture was dominated by "Candidatus Chlorohelix allophototropha" strain L227-S17, a phototrophic Chloroflexota member that uses a Type I photosynthetic reaction center, and the putative iron-reducing bacterium Geothrix strain L227-G1.
description
Canada: near Kenora, Ontario, Lake 227, IISD-Experimental Lakes Area
geo_loc_name
Ca. Chlorohelix allophototropha enrichment, subculture 22.2b; a bacterial enrichment culture of water from the upper anoxic zone (3.88 m depth) of a Boreal Shield lake
isolation_source
49.69 N 93.69 W
lat_lon
freshwater metagenome
http://purl.obolibrary.org/obo/NCBITaxon_449393
organism
anaerobe
rel_to_oxygen
Initial inoculum: anoxic water was pumped to the surface through a flushed line using a gear pump. Water was pumped directly into 120 mL glass serum bottles that were allowed to overflow for several seconds and then quickly sealed using rubber stoppers.
samp_collect_device
Handling of the initial inoculum: water was kept chilled during storage and shipping to the laboratory. Water was then distributed anoxically into glass serum bottles, amended with a ferrous iron-containing freshwater minimal medium, and incubated under light.
samp_mat_process
Ca. Chlorohelix allophototropha enrichment, subculture 22.2b
title