SAMN29720337
External Id
University of Cambridge
INSDC center name
2023-07-02T00:00:00Z
INSDC first public
2023-07-02T01:11:29.290Z
INSDC last update
SRS13848392
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
SRS13848392
SRA accession
HEK293T, mESC
cell line
cultured cells
cell type
missing
collection_date
WT/WT
genotype
missing
geo_loc_name
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
organism
kidney, Embryo
source_name
kidney, Embryo
tissue
spindrop mES HEK293T species mixing nuclei
title
HEK293T cultured in DMEM supplemented with 10% FBS, mESC cells cultured in 2i/LIF. HEK293T were dissociated with Trypsin-EDTA for 5 minutes at 37C , mESCs were dissociated with Accutase. Nuclei were recovered using the Nuclei EZ Prep kit and pooled at equal concentrations and stained with Vybrant Green before being processed using spinDrop.
treatment