SAMN36349481
External Id
Ginkgo Bioworks
INSDC center name
2023-07-07T00:00:00Z
INSDC first public
2023-07-07T11:00:43.358Z
INSDC last update
live
INSDC status
SARS-CoV-2: wastewater surveillance
NCBI submission model
SARS-CoV-2.wwsurv.1.0
NCBI submission package
Pennsylvania Department of Health
collected_by
2023-04-12
collection_date
School 001
collection_site_id
2:25:00 PM
collection_time
250 mL
collection_volume
membrane filtration with sample acidification
concentration_method
Wastewater from school campus
description
Negative extraction control - di water or tween twenty buffer
extraction_control
LuminUltra Wastewater Extraction Kit
extraction_method
USA: Pennsylvania
geo_loc_name
wastewater
isolation_source
wastewater metagenome
http://purl.obolibrary.org/obo/NCBITaxon_527639
organism
Analysis of wastewater samples from manhole level collection on school campus
project_name
public health surveillance building-level
purpose_of_ww_sampling
SARS-CoV-2 variant sequencing
purpose_of_ww_sequencing
SARS-CoV-2: wastewater surveillance sample from wastewater metagenome
title
Pepper mild mottle virus (PMMoV)
ww_endog_control_1
21,632,865.31
ww_endog_control_1_conc
Kitajima, M., Sassi, H.P. & Torrey, J.R. Pepper mild mottle virus as a water quality indicator. npj Clean Water 1, 19 (2018). https://doi.org/10.1038/s41545-018-0019-5
ww_endog_control_1_protocol
copies/L
ww_endog_control_1_units
600
ww_population
School team
ww_population_source
8
ww_sample_duration
raw wastewater
ww_sample_matrix
school
ww_sample_site
composite
ww_sample_type
PA
ww_surv_jurisdiction
SARS-CoV-2
ww_surv_target_1
388.28
ww_surv_target_1_conc
copies/L
ww_surv_target_1_conc_unit
N gene (N1)
ww_surv_target_1_gene
no
ww_surv_target_1_known_present
Falzone, L., Musso, N., Gattuso, G., Bongiorno, D., Palermo, C. I., Scalia, G., Libra, M., Stefani, S.. Sensitivity assessment of droplet digital PCR for SARS-CoV-2 detection. International Journal of Molecular Medicine 46, no. 3 (2020): 957-964. https://doi.org/10.3892/ijmm.2020.4673"
ww_surv_target_1_protocol