SAMN32246270
External Id
Instituto Superior Tecnico
INSDC center name
2023-07-24T00:00:00Z
INSDC first public
2023-07-24T05:18:02.695Z
INSDC last update
SRS16150307
INSDC secondary accession
live
INSDC status
MIGS/MIMS/MIMARKS.miscellaneous
NCBI submission model
MIMARKS.survey
NCBI submission model
MIMARKS.survey.miscellaneous.6.0
NCBI submission package
SRS16150307
SRA accession
2019-04-04
collection_date
Keywords: GSC:MIxS;MIMARKS:6.0
description
environmental material ENVO:01000998
env_broad_scale
not applicable
env_local_scale
not applicable
env_medium
Portugal: Lisbon
geo_loc_name
Biocolonization brownish-blackish stain (BBS) on the surface of a NE oriented facade in site B (area2 building)
isolation_source
not applicable
lat_lon
surface metagenome
http://purl.obolibrary.org/obo/NCBITaxon_1774230
organism
Surface scrapping with a sterile spatula (-R)
samp_collect_device
Total DNA extracted with the NucleoSpin Soil DNA Extraction kit (Mackerey-Nigel, Germany); ITS amplicons flanked by Illumina universal primers obtained with hybrid primers ITS1f and ITS2 and PlatinumTM II Hot-Start PCR Master Mix were sequenced using the MiSeq Illumina platform.
samp_mat_process
B-BBS5-R
source_material_id
B-BBS5-R
title