SAMN14918466
External Id
University of Bremen
INSDC center name
2020-05-14T00:00:00Z
INSDC first public
2023-07-28T21:07:59.754Z
INSDC last update
SRS6658890
INSDC secondary accession
live
INSDC status
MIMAG
NCBI submission model
MIMAG.6.0
NCBI submission package
SRS6658890
SRA accession
This BioSample is a metagenomic assembly obtained from the marine sediment metagenome BioSamples: SAMN14820614, SAMN14820615, and SAMN14820616
derived-from
Keywords: GSC:MIxS;MIMAG:6.0
description
1
environmental sample
North Sea: German Bight (Helgoland Mud Area)
geo loc name
BM5_bin.17
isolate
marine sediment
isolation source
54.052300 N 7.580400 E
lat lon
marine sediment metagenome
metagenome source
1
metagenomic
Actinomycetes bacterium
http://purl.obolibrary.org/obo/NCBITaxon_1883427
organism
obligate anaerobe
rel_to_oxygen
Enrichment was prepared using 5 mL volume from 4th transfer as starting material in a final volume of 50 mL widdel medium. Sample name identifies the unique information of each sample including the target 16S rRNA gene and the phase of the enrichment transfer.
samp mat process
5 mL enrichment was centrifuged down to 1 mL volume which was used for DNA extraction
samp_size
metagenomic assembly
sample-type
Benzoate + magnetite enrichment at 30ºC after the fifth transfer completely metabolised benzoate coupled to magnetite reduction. 5mL was collected from the enrichment bottle and stored at -20ºC prior to DNA extraction and metagenome sequencing. Pooled DNA from three replicates of the BM5 enrichment used for metagenome sequencing. BM5_bin.17 is the reassembled metagenome assembled genome
source_material_id
MIMAG Metagenome-assembled Genome sample from Actinomycetes bacterium
title