SAMN36871502
External Id
University of Cambridge
INSDC center name
2023-08-10T00:50:33.790Z
INSDC first public
2023-08-10T00:50:33.790Z
INSDC last update
SRS18529512
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
SRS18529512
SRA accession
CD-1
cell line
embryos
cell type
missing
collection_date
missing
geo_loc_name
Mus musculus
http://purl.obolibrary.org/obo/NCBITaxon_10090
organism
Embryos at E8.5 whole embryo
source_name
Embryos at E8.5 whole embryo
tissue
spindrop mouse nascent RNA 5EU-seq sample 1
title
Sample was dissected and cultured for 3 hours in IVC1 with the 5EU analog, cells were then dissociated with TrypLE Express for 15 minutes at 37C, Click-chemistry was performed as per the scEU-seq protocol and cells were processed using spindrop without sorting. After de-emulsification and clean-up the "nascent" samples are eliuted from the streptavidin beads before downstream library preparation.
treatment