SAMN30747290
External Id
James Ellis, Molecular Genetics, University of Toronto
INSDC center name
2023-09-01T00:00:00Z
INSDC first public
2023-09-01T01:18:20.317Z
INSDC last update
SRS15052062
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
SRS15052062
SRA accession
line 37 (Cheung 2011, HMG)
cell line
missing
collection_date
Sequencing libraries were made using the Small RNA library preparation kit NEBNext (NEB) according to the manufacturer’s instructions. Sequencing was performed on the Illumina HiSeq 2500 using the Rapid Run mode.
extract_protocol
To calculate relative and absolute differences in the miRNA population in NEUWT and NEURTT, small RNAs were extracted from two replicates of both lines using the same number of cells followed by the addition of a set of spike-in RNAs. Small RNAs were extracted from 500,000 neurons of each line using the SPLIT RNA extraction Kit (Lexogen) according to the manufacturer’s instructions. A set of 52 RNA spike-ins (QIAseq miRNA Library QC Spike-Ins – Qiagen) that spanned a wide range of concentrations were added to the recovered RNAs according to the manufacturer’s instructions.
extraction protocol
wild type
genotype
missing
geo_loc_name
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
organism
C
sequencing batch
iPSC derived neuronal progenitors
source_name
miRNA-seq NPC WT rep 2
title
NA
treatment
NA
treatment protocol