pooled DNA
ERS3098873
SRA accession
Dongxiao Sun
collected by
cattle
common name
DNAmixture of 44 semens - The sample was the mixture of DNA extracted from 44 Chinese Holstein bulls semen
description
Bos taurus
http://purl.obolibrary.org/obo/NCBITaxon_9913
organism
Bos taurus
http://purl.obolibrary.org/obo/NCBITaxon_9913
scientific name
male
http://purl.obolibrary.org/obo/PATO_0000384
sex
We designed and synthesized 23 pairs of primers in the entire exons, and 3 kb of 5′ and 3′ flanking regions of bovine LIPJ gene according to the genomic sequences of (GenBank accession number: NC_037353.1). 44 semen DNAs were pooled to use for the PCR amplifications with the equal concentration (50 ng/µL) for each DNA. All the PCR products were sequenced by ABI3730XL DNA analyzer (Applied Biosystems, Foster, CA, USA). Then, we aligned the sequences with the reference genome (UMD3.1.1) using the BLAST (https://blast.ncbi.nlm.nih.gov/Blast.cgi), and identified the novel SNP, g.10250120A/G.
submission description
GSB-702
submission identifier
the National Science and Technology Programs of China
submission title