ERS3375340
SRA accession
Jiangsu, China by Ward, R., Michigan State University; Jiang, G., Nanjing Agricultural University; Huang, D., Shanghai Academy of Agricultural Sciences; Shen, Q., Zhejiang Academy of Agricultural Sciences; Yang, Z., Anhui Academy of Agricultural Sciences; Lu, W., Jiangsu Academy of Agricultural Sciences; Shi, J., Jiangsu Academy of Agricultural Sciences; Zhu, H., Nanping Institute of Agricultural Sciences; Chen, Z., Nanjing Agricultural University. DONATED 10/26/2005 by Jiang, G., Michigan State University
collected by
bread wheat
common name
CJ 9311
cultivar
USDA GRIN
culture collection
exome sequences of ExomeCapture-DAS5-001309 - Accession Name = 'CJ 9311'; Remarks = 'Awned, semi-dwarf and red-grained spring type with red glumes. Has a high level of resistance to Fusarium head blight (FHB or scab) caused by Fusarium graminearum. Scores for disease severity (such as number of scabby spikelets) under single-floret inoculation were consistently lower than the resistant check Sumai 3 or Ning 7840. Under a natural epidemic of scab, CJ 9311 scores for disease index were close to those for Sumai 3. Superior to Sumai 3 in the resistance to disease spread of wheat scab, and has similar comprehensive resistance to Sumai 3. Under field conditions at Nanjing, China, plant height was around 90 cm (30 cm shorter than Sumai 3). Spike length, spikelets and kernels per spike were around 11 cm, 21-22 and 38-39, respectively (10 cm, 21 and 34-36 for Sumai 3). Grains are red, oval, semi-soft and plump. 1000 kernel and volume weights were 34 g and 756 g/l, better than those of Sumai 3 (33 g and 750 g/l). Biomass per stalk, grain yield/spike and harvest index were 3.5 g, 1.4 g and 39.7%, compared to Sumai 3 (3.2 g, 1.1 g and 35.1%). Duration of growth is around 210 d in Nanjing. Was 1-2 d earlier in heading/flowering and more lodging-resistant than Sumai 3. Was moderate to rusts and powdery mildew.'; Released = '.'; Lattitude,Longitude,Elevation = '.,.,.'; Ancestry = 'Originally derived from a C3 population of scab-resistant gene pool generated by intermating among hybrids and progenies of 75 crosses with five agronomic parents (Yangmai 4, Ningmai 3, Zhemai 1, Qianjiang 1 and Wanjian 7909) crossed to each of 15 scab-resistant and/or complementary parents (Sumai 3, Ning 7840, Wangshuibai, Whengzhouhongheshang, Fanshanxiaomai, Emai 9, Zhen 7495, Jinzhou 1, Xiangmai 1, Nobeokabouzu-Komogi, Shinchunaga, Qianhuan 803, 089xAvrora, Frontana and Veery), with the aid of a dominant male-sterile gene Ta1 (ms2), and bulking the seeds from the male-sterile plants, followed by recurrent selection.'
description
2 week old
dev stage
spring wheat
ecotype
China
geographic location (country and/or sea)
PI 641166
material
Triticum aestivum
organism
Triticum aestivum subsp. aestivum
scientific name
USDA GRIN:PI 641166
specimen voucher
subsp. aestivum
sub species
The exome sequenced hexaploid wheat accessions were selected from a worldwide population of landraces and cultivars obtained from the USDA National Small Grains Collection and the Australian Grains Genebank (formally the Australian Winter Cereal Collection). The worldwide population comprised 3990 accessions from 106 countries that were genotyped using the Infinium iSelect 90K SNP wheat bead chip array. The MCG method was used to select accessions for exome sequencing. This method uses a genetic relationship matrix calculated from genotype data to iteratively chose and arbitrary number of accessions (in the case of this study 20% of the accessions) that collectively explain the largest proportion of variance in genetic relationships among the whole set. Genomic DNA was extracted from leaf tissue for each accession using the Agencourt DNAdvance Genomic DNA Isolation Kit (Beckman Coulter) and subjected to sequence capture using the NimbleGen SeqCap EZ wheat whole genome assay. In brief, 1 µg of DNA was fragmented with the Covaris S2 instrument to obtain an average fragment length of 300 bp. Shorter fragments were removed using Agencourt AMPure XP beads (Beckman Coulter). The KAPA Library Preparation Kit (Kapa Biosystems) and adapters supplied in the NimbleGen SeqCap EZ Reagent Kit Plus v2 (Roche) were used to prepare sample libraries for the capture assay, according to the KAPA protocol but excluding steps 8.1-8.22. The quality and yield of each sample library was assessed using an Agilent 2200 TapeStation (Agilent Technologies) before proceeding to the sequence capture assay following the Roche protocol. Each target capture sequence enriched sample library was sequenced on an Illumina HiSeq2000 instrument (Illumina) to generate about 30 million reads per accession. The project leaders are Matthew Hayden (Agriculture Victoria, AgriBio, Centre for AgriBioscience, Bundoora, Victoria, Australia; La Trobe University, Bundoora, Victoria, Australia) and Eduard Akhunov (Kansas State University, USA). More information can be found on our project website at http://wheatgenomics.plantpath.ksu.edu/1000EC
submission description
GSB-804
submission identifier
Exome sequencing of diverse collection of wheat landraces, cultivars and breeding lines
submission title
leaf
http://purl.obolibrary.org/obo/PO_0025034
tissue type
:CJ 9311;:Changjiang 9311
variety