SAMN15596640
External Id
Hellenic Centre of Marine Research (HCMR)
INSDC center name
2022-08-01T00:00:00Z
INSDC first public
2023-10-26T21:06:30.370Z
INSDC last update
SRS7082304
INSDC secondary accession
live
INSDC status
MIGS.eu
NCBI submission model
MIGS/MIMS/MIMARKS.host-associated
NCBI submission model
MIGS.eu.host-associated.6.0
NCBI submission package
SRS7082304
SRA accession
2015-08-01/2015-08-08
collection_date
Keywords: GSC:MIxS;MIGS:6.0
description
mediterranean sea biome [ENVO_01000047]
env_broad_scale
environment associated with an aquatic invertebrate [ENVO_01001176]
env_local_scale
Protula[NCBITaxon_51281]|coastal sea water [ENVO_00002150]
env_medium
Spain:Granada
geo_loc_name
Protula
host
Protula intestinum, Aphia ID: 131032
host_body_habitat
PMID: 32392868
isol_growth_condt
coastal sea water
isolation_source
36.723083 N 3.726417 W
lat_lon
Hypocreales sp. ML197-S3
http://purl.obolibrary.org/obo/NCBITaxon_2758378
organism
host collection: scuba diving
samp_collect_device
After sampling, the invertebrate was transferred carefully to the laboratory to be processed. Small pieces of tissue were taken from the sample before freezing. 1 cm3 samples were ground in sterile seawater and heated at 50C for 1 h. The suspension was serially diluted, plated on Difco Marine Broth Agar (BD Biosciences San Jose, CA, USA), and incubated at 28C for six weeks. A single colony was picked from the agar and cultivated as a pure culture on Difco Potato Dextrose Agar and Difco Marine Broth Agar (BD Biosciences San Jose, CA, USA) (media and cultivated for five days at 28C. The strain spores and mycelium were recovered by a gentle scratch of the agar plate surface using a scalpel, and they were conserved at 20C in 10% glycerol solution.
samp_mat_process
sea water salinity: 38
samp_salinity
host tissue: -20C, fungal isolate: -80C
samp_store_temp
ML197-S3
strain
sea water temperature: 21C - 27C
temp
MIGS Eukaryotic sample from Hypocreales sp. ML197-S3
title