SAMN23341843
External Id
Genetics, Stanford University
INSDC center name
2023-11-01T00:00:00Z
INSDC first public
2023-11-01T03:19:35.550Z
INSDC last update
SRS11111279
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
SRS11111279
SRA accession
Old
age-young/old
Mus musculus
http://purl.obolibrary.org/obo/NCBITaxon_10090
organism
NSCs in vitro
source_name
C57BL/6N
strain
NSCs in vitro
tissue/cell type
screen 1/2, old, quiescent [SC36_O_Q]
title
Whole genome sgRNA library
treatment
sgRNA lentivirus library was freshly thawed at room temperature and diluted 1:5 in Neurobasal media and then B27 and growth factors were added to make it qNSC media, and 18 mL of this mix was added to plates for 16-hour overnight infection. The virus dilution added was based on viral titering experiments determined to achieve ~30% culture infection of cells to ensure each cell only received a single sgRNA
treatment