SAMN30170958
External Id
Shanghai veterinary research institute
INSDC center name
2023-09-01T00:52:36.550Z
INSDC first public
2023-09-01T00:52:36.550Z
INSDC last update
live
INSDC status
Microbe, viral or environmental
NCBI submission model
Microbe.1.0
NCBI submission package
ERS16749728
SRA accession
2021-02-01
collection_date
Gene stability assessment of ASFV-GZdeltaI177LCD2vMGF in vitro and in vivo. To this end, the vaccine virus was mixed with the same dose of wild strain in equal volume (104.0 TCID50 in 500ul), and then infected BMDM cells at a multiple of infection (m.o.i) of 0.01. After 72 h post-infection, the presence of fluorescence was detected under a fluorescence microscope (ECHO Global) and the proportion of infected cells was calculated. The virus culture was harvested for four more passages in BMDM cells at a 2 % (V/V) dose. we purified the fifth-generation virus by plaque purification and limiting dilution, followed by nanopore sequencing to evaluate its gene stability.
description
China
geo_loc_name
Sus scrofa
host
African swine fever virus
http://purl.obolibrary.org/obo/NCBITaxon_10497
organism
cell culture
sample_type
Asfavirus
strain
Microbe sample from African swine fever virus
title