SAMN29379773
External Id
University of Michigan
INSDC center name
2023-08-15T00:50:27.017Z
INSDC first public
2023-08-15T00:50:27.017Z
INSDC last update
SRS13718748
INSDC secondary accession
live
INSDC status
Metagenome or environmental
NCBI submission model
Metagenome.environmental.1.0
NCBI submission package
SRS13718748
SRA accession
2020-12-21
collection_date
Wastewater raw influent grab sample from December 21, 2020 concentrated and purified for viruses to validate a quantitative metagenomic method using spike-in standards (replicate 1/3).
description
USA: Ann Arbor, MI
geo_loc_name
wastewater raw influent
isolation_source
42.2693760 N 83.6643886 W
lat_lon
wastewater metagenome
http://purl.obolibrary.org/obo/NCBITaxon_527639
organism
post bar screen grab sample from autosampler
samp_collect_device
virus concentration with ultrafiltration (tangential and dead-end) and virus purification with 0.45-um filter, chloroform treatment, and DNase treatment; viral DNA extraction with QIAamp UltraSens Virus Kit (QIAGEN, Cat. No. 53706); spiked in synthetic dsDNA (metagenome mix A from sequinstandards.com/metagenome/) and ssDNA standards for quantitative metagenomics
samp_mat_process
10.7 L
samp_size
wastewater virome
source_material_id
Viral Metagenome from Wastewater Raw Influent
title