SAMN33725928
External Id
California Institute of Technology
INSDC center name
2023-08-17T00:50:16.717Z
INSDC first public
2023-08-17T00:50:16.717Z
INSDC last update
SRS17008390
INSDC secondary accession
live
INSDC status
Human
NCBI submission model
Human.1.0
NCBI submission package
SRS17008390
SRA accession
not applicable
age
ATCC CRL-3216 www.atcc.org
biomaterial_provider
HEK293T (CRL-3216)
cell_line
ATCC:CRL-3216
culture_collection
HEK293 cell populations were prepared with puromycin or zeocin resistance and clonally labeled using genetic barcodes delivered by lentivirus. 5,000 cells of each drug-resistant population were sorted together into a well, then cultured for 5 days. Supernatant containing exported RNA was collected at 24 hour intervals. RNA barcodes were reverse transcribed, amplified by PCR, and sequenced. This sample was collected at day 3 of growth under zeocin selection.
description
HEK293T
isolate
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
organism
1
replicate
RNA barcode exported by cells in cell culture
sample_type
female
sex
3
time
embryonic kidney
tissue
Human sample from Homo sapiens
title
zeocin selection
treatment