SAMN33905432
External Id
University of Arkansas for Medical Sciences
INSDC center name
2023-08-29T00:00:00Z
INSDC first public
2023-08-29T12:55:02.288Z
INSDC last update
SRS17131066
INSDC secondary accession
live
INSDC status
MIGS/MIMS/MIMARKS.host-associated
NCBI submission model
MIMARKS.survey
NCBI submission model
MIMARKS.survey.host-associated.6.0
NCBI submission package
SRS17131066
SRA accession
V3V4
amplicon_region
saline (0.9% sodium chloride)
chem_administration
2020-05-18
collection_date
Saline treatment, mouse 201
description
gut(UBERON:0001007)
env_broad_scale
colon(UBERON:0001155)
env_local_scale
feces(UBERON:0001988)
env_medium
CCTACGGGNGGCWGCAG
forward_primer
USA:Arkansas,Little Rock (GAZ,00165676)
geo_loc_name
Mus musculus (C57/BL6)
host
Gastrointestinal
host_body_habitat
feces
host_body_product
ad libitum availability to drinking water and standard chow
host_diet
standard chow
host_last_meal
adult
host_life_stage
female
host_sex
201
host_subject_id
10090
host_taxid
34.7504 N 92.3227 W
lat_lon
mouse gut metagenome
http://purl.obolibrary.org/obo/NCBITaxon_410661
organism
Intraperitoneal injections on days 1, 8, 15, and 22 with saline (0.9% sodium chloride)
perturbation
GACTACHVGGGTATCTAATCC
reverse_primer
Fecal samples were collected from individual mice and stored at -80C. Fecal pellets were shipped to RTL Genomics (Lubbock, Texas) for DNA extraction and 16S rRNA gene sequencing using their "MiSeq Diversity Assay" targeting the V3V4 hypervariable region, with the primers (357wF: CCTACGGGNGGCWGCAG - 785R: GACTACHVGGGTATCTAATCC).
samp_mat_process
-80 C
samp_store_temp
V3V4 16S rRNA gene amplicon sequences were provided by RTL Genomics (Lubbock, Texas).
sequencing_provider
Saline Immediate Mouse 201
title