SAMN33725950
External Id
California Institute of Technology
INSDC center name
2023-08-17T00:50:14.013Z
INSDC first public
2023-08-17T00:50:14.013Z
INSDC last update
SRS17008381
INSDC secondary accession
live
INSDC status
Human
NCBI submission model
Human.1.0
NCBI submission package
SRS17008381
SRA accession
not applicable
age
ATCC CRL-3216 www.atcc.org
biomaterial_provider
HEK293T (CRL-3216)
cell_line
ATCC:CRL-3216
culture_collection
To characterize the sensitivity of the RNA export-based population dynamics reporter system, HEK293 cell populations were clonally labeled using genetic barcodes delivered by lentivirus. 10 labeled cells were sorted into a well together with a carrier population of 29,990 HEK293T cells. Culture supernatant was harvested 24 hours after sorting. RNA was extracted from this supernatant and RNA barcodes were reverse transcribed, amplified by PCR, and sequenced. This sample is from well 6 of 8 replicate wells.
description
HEK293T
isolate
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
organism
6
replicate
RNA barcode exported by cells in cell culture
sample_type
female
sex
1
time
embryonic kidney
tissue
Human sample from Homo sapiens
title
characterizing sensitivity
treatment