SAMN32643692
External Id
Michigan State University
INSDC center name
2023-09-05T00:00:00Z
INSDC first public
2023-09-05T22:16:03.713Z
INSDC last update
SRS16403756
INSDC secondary accession
live
INSDC status
Plant
NCBI submission model
Plant.1.0
NCBI submission package
SRS16403756
SRA accession
4-week-old
age
Col-3 plants were treated with 10 mM MgCl2, serving as a mock control
description
Col-3
ecotype
tgnap1-2
genotype
USA: East Lansing
geo_loc_name
Surface-sterilized seeds were plated on half-strength Linsmaier Skoog (LS) medium (Caisson Labs, Ontario, Canada) supplemented with 1% sucrose (Sigma-Aldrich, St. Louis, MO, USA), and 1.2% Agar (Acumedia, Lansing, MI, USA). Appropriate antibiotics was also added for screening of transgenic lines. After stratification in the dark at 4 °C for 2 days, plates were transferred to a controlled growth chamber with 80 μmol m−2 s−1 under 16 h light:8 h dark with temperature 22 °C.
growth_protocol
Arabidopsis thaliana
http://purl.obolibrary.org/obo/NCBITaxon_3702
organism
Susceptible to pathogen infection
phenotype
Whole seedlings
tissue
tnap1-2_DMSO
title
Treatmetn with 10 mM MgCl2 (mock)
treatment