SAMN34415832
External Id
Trinity College Dublin
INSDC center name
2023-10-30T00:00:00Z
INSDC first public
2023-10-30T06:46:01.517Z
INSDC last update
SRS17453880
INSDC secondary accession
live
INSDC status
MIGS/MIMS/MIMARKS.human-associated
NCBI submission model
MIGS.eu
NCBI submission model
MIGS.eu.human-associated.6.0
NCBI submission package
SRS17453880
SRA accession
not applicable
collection_date
Keywords: GSC:MIxS;MIGS:6.0
description
not applicable
env_broad_scale
not applicable
env_local_scale
not applicable
env_medium
not applicable
geo_loc_name
Homo sapiens
host
DNA was extracted using the using the MasterPure™ Yeast DNA Purification Kit (VWR, CAT# 76081-694) with the optional RNase A treatment. gDNA concentrations were quantified using the Qubit dsDNA Broad Range Assay Kit (ThermoFisher). Following quantification, the samples were used to prepare libraries using the NEBNext Ultra II FS DNA Library Preparation Kit (New England BioLabs). Briefly, 500 ng of input gDNA was fragmented to a size of 300-700 bp, followed by dual indexing using Illumina TruSeq adaptors. Fragments were then size selected for final library sizes of 470-800 bp, followed by 3 cycles for PCR enrichment.
isol_growth_condt
not applicable
lat_lon
Candida albicans
http://purl.obolibrary.org/obo/NCBITaxon_5476
organism
MAY1244
strain
C. albicans leu2/LEU2 strain
title