SAMN35750888
External Id
Developmental Genetics, Max Planck Institute for Molecular Genetics
INSDC center name
2023-10-31T00:00:00Z
INSDC first public
2023-10-31T15:29:08.015Z
INSDC last update
SRS17981190
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
SRS17981190
SRA accession
genetically modified F1G4 (C57BL/6N x SV129) mES cells
cell line
notochord progenitors
cell type
missing
collection_date
T::H2B-Venus (BAC), Noto::H2B-mCherry (BAC), Foxa2::H2B-mTurquoise (BAC)
genotype
missing
geo_loc_name
Mus musculus
http://purl.obolibrary.org/obo/NCBITaxon_10090
organism
FACS sorted Noto+/T+/Foxa2+ from caudal ends of TS12/TS13 embryos obtained from tatraploid complementation of embryonic stem cells
source_name
FACS sorted Noto+/T+/Foxa2+ from caudal ends of TS12/TS13 embryos obtained from tatraploid complementation of embryonic stem cells
tissue
ATAC NotoP
title
In order to obtain single cell suspensions from embryo tissue for FACS sorting, 10ul of 5% BSA in PBS was added to the pooled samples and 100ul of ice-cold 0.05% trypsin was added. A homogenous suspension was achieved by slow pipetting and the reaction was stopped with the addition of 200ul of 5% BSA in PBS. Before cell sorting on a FACS Aria II (Becton Dickinson), the cell suspension was filtered through a 35µm mesh. Cells were sorted into FACS-tubes containing 5% BSA in PBS, which had previously been rotating for several hours to pre-coat the tube walls with BSA. Sorted cells were centrifuged at 1000rpm and 4°C for 10 minutes, transferred to Eppendorf tubes containing PBS with 0.05% Triton X-100 and centrifuged at 1000rpm and 4°C for 10 minutes. The supernatant was removed and cells were directly processed for ATAC-Seq.
treatment