SAMN35750873
External Id
Developmental Genetics, Max Planck Institute for Molecular Genetics
INSDC center name
2023-10-31T00:00:00Z
INSDC first public
2023-10-31T15:29:06.961Z
INSDC last update
SRS17981205
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
SRS17981205
SRA accession
genetically modified F1G4 (C57BL/6N x SV129) mES cells
cell line
notochord progenitors
cell type
missing
collection_date
T::H2B-Venus (BAC), Noto::H2B-mCherry (BAC), Foxa2::H2B-mTurquoise (BAC)
genotype
missing
geo_loc_name
Mus musculus
http://purl.obolibrary.org/obo/NCBITaxon_10090
organism
FACS sorted Noto+/T+/Foxa2+ from caudal ends of TS12 embryos obtained by tetraploid complementation assays
source_name
FACS sorted Noto+/T+/Foxa2+ from caudal ends of TS12 embryos obtained by tetraploid complementation assays
tissue
RNA NotoP E8.5
title
In order to obtain single cell suspensions from embryo tissue for FACS sorting, 10ul of 5% BSA in PBS was added to the pooled samples and 100ul of ice-cold 0.05% trypsin was added. A homogenous suspension was achieved by slow pipetting and the reaction was stopped with the addition of 200ul of 5% BSA in PBS. Before cell sorting on a FACS Aria II (Becton Dickinson), the cell suspension was filtered through a 35µm mesh. Cells were sorted directly into Eppendorf tubes containing 350µl RLT buffer (Qiagen) and 3.5ul b-mercaptoethanol.
treatment