SAMN35750887
External Id
Developmental Genetics, Max Planck Institute for Molecular Genetics
INSDC center name
2023-10-31T00:00:00Z
INSDC first public
2023-10-31T15:29:06.489Z
INSDC last update
SRS17981191
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
SRS17981191
SRA accession
genetically modified F1G4 (C57BL/6N x SV129) mES cells
cell line
in vitro generated notochord progenitor-like cells
cell type
missing
collection_date
TRE::Foxa2-IRES-EGFP (Rosa26 locus); T::H2B-mCherry-T2A-irTA (BAC); Noto::H2B-mTurquoise (BAC)
genotype
missing
geo_loc_name
Mus musculus
http://purl.obolibrary.org/obo/NCBITaxon_10090
organism
in vitro differentiated cell cultures
source_name
in vitro differentiated cell cultures
tissue
ChIP Foxa2 NotoPdiff
title
Crosslinking was performed directly on differentiating cells in growth medium with the addition of 1/10th volume of crosslinking solution (11% formaldehyde, 100mM NaCl, 1mM EDTA pH8, 0.5mM EGTA pH8, 50mM Hepes pH7.8) for 10 minutes at room temperature, with the dishes placed on a shaker. The crosslinking reaction was quenched with the addition of 1/10th volume of 2.5M glycine and 5 minutes incubation. Dishes were placed on ice or in a 4°C cold room and washed twice with cold PBS. Cells were scraped and washed off using cold PBS, containing 0.05% Triton X-100 to break the surface tension. Cells were pooled in aliquots of ~30-50x10^6 in Eppendorf tubes, snap frozen and stored at -80°C until sonication.
treatment