SAMN35750875
External Id
Developmental Genetics, Max Planck Institute for Molecular Genetics
INSDC center name
2023-10-31T00:00:00Z
INSDC first public
2023-10-31T15:29:07.177Z
INSDC last update
SRS17981203
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
SRS17981203
SRA accession
genetically modified F1G4 (C57BL/6N x SV129) mES cells
cell line
in vitro generated mesodermal progenitors
cell type
missing
collection_date
TRE::Foxa2-IRES-EGFP (Rosa26 locus); T::H2B-mCherry-T2A-irTA (BAC); Noto::H2B-mTurquoise (BAC)
genotype
missing
geo_loc_name
Mus musculus
http://purl.obolibrary.org/obo/NCBITaxon_10090
organism
FACS sorted T+/Noto- cells from in vitro differentiated cultures
source_name
FACS sorted T+/Noto- cells from in vitro differentiated cultures
tissue
RNA MPdiff
title
A single cell suspension of in vitro differentiated cells was filtered through a 35µm mesh before cell sorting on a FACS Aria II (Becton Dickinson), the cell suspension was filtered through a 35µm mesh. Cells were sorted directly into Eppendorf tubes containing 350µl RLT buffer (Qiagen) and 3.5ul b-mercaptoethanol.
treatment