SAMN35571667
External Id
CUCEI-Universidad de Guadalajara
INSDC center name
2023-06-07T00:00:00Z
INSDC first public
2023-09-05T17:09:21.111Z
INSDC last update
live
INSDC status
MIMARKS.survey
NCBI submission model
MIGS/MIMS/MIMARKS.wastewater
NCBI submission model
MIMARKS.survey.wastewater.6.0
NCBI submission package
ERS16868786
SRA accession
Alpha factor form 0.3 to 1.2
alkalinity
35 and 15 g/L
chem_oxygen_dem
2019-02-12/2020-04-16
collection_date
The aim of this study was to characterize the putative prokaryotic interactions involved in methane production during the transition from mesophilic to thermophilic conditions in a fixed bed reactor (FBR), applying two control schemes based on alkalinity alpha factor to acclimate microbial populations. The first scheme control applied was implemented to adapt the feeding flow of tequila vinasses to achieve digester stability under mesophilic conditions. The second one was used to adapt the temperature in the FBR, keeping the feeding flow of diluted tequila vinasses constant to test the acclimation of the Archaea and Bacteria communities from mesophilic to thermophilic conditions. Biomass samples from inoculum (U3) and biofilms (U5, U99, U105, U113, and U119) were collected and processed for genomic DNA extraction. Thereafter, 16S rDNA from DNA samples were sequenced by high throughput Illumina MiSeq for bacterial and archaeal communities. Biological data obtained was analyzed to characterize abundance, diversity, and population dynamics of methane-producing communities present in a FBR digester fed with tequila vinasses under mesophilic and thermophilic conditions.
description
Microbiome
env_broad_scale
Anaerobic digester
env_local_scale
Tequila vinasses
env_medium
Biogas volume from 3.0 to 6.9
gaseous_substances
Mexico: Guadalajara
geo_loc_name
PVC tube
isolation_source
20.75 N 103.40 W
lat_lon
bioreactor metagenome
http://purl.obolibrary.org/obo/NCBITaxon_1076179
organism
6.8-7.4
ph
Fixed-bed reactor
reactor_type
anaerobe
rel_to_oxygen
QIAamp DNA Mini Kit
samp_mat_process
20 mL for suspended biomass and 5 mL for biofilms
samp_size
3 months
samp_store_dur
Freezer
samp_store_loc
0.05 mL
samp_vol_we_dna_ext
Laboratory reactor
sewage_type
Sample were centrifuged and the centrifuged biomass was processed
size_frac
Fixed-bed reactor biofilm
source_material_id
Methane-producing communities from anaerobic reactors fed with tequila-manufacturing residues
title
Tequila vinasses
wastewater_type