SAMN19717605
External Id
Deutsches Rheuma-Forschungszentrum
INSDC center name
2023-11-09T00:00:00Z
INSDC first public
2023-11-09T08:05:05.582Z
INSDC last update
SRS9220257
INSDC secondary accession
live
INSDC status
Metagenome or environmental
NCBI submission model
Metagenome.environmental.1.0
NCBI submission package
SRS9220257
SRA accession
2020-04-28
collection_date
Swab sample from COVID-19 patient with severe symptoms.
description
Oral swab sticks were washed with PBS buffer and the DNA of captured bacteria isolated with the Quick-DNA™ Fecal/Soil Microbe Miniprep Kit. 5 µL of the obtained DNA were subjected to the Illumina Nextera® XT workflow using the primers 5´-TCgTCggCAgCgTCAgATgTgTATAAgAgACAgCCTACgggNggCWgCAg-3’ and 5´-gTCTCgTgggCTCggAgATgTgTATAAgAgACAggACTACHVgggTATCTAATCC-3’ for the first amplicon PCR with 45 PCR cycles. After Nextera® v2 index PCR and quality control by capillary gel electrophoresis samples were pooled and sequenced on MiSeq™ device (Illumina).
design_description
Cov_2_012
donor
Germany: Berlin
geo_loc_name
Homo sapiens
host
SEVERE
host diseace
human_swab
isolation_source
52.52 N 13.37 E
lat_lon
human nasopharyngeal metagenome
http://purl.obolibrary.org/obo/NCBITaxon_1131769
organism
Surek@drfz.de durek COVID-19 : hup
title