SAMN19717666
External Id
Deutsches Rheuma-Forschungszentrum
INSDC center name
2023-11-09T00:00:00Z
INSDC first public
2023-11-09T08:05:07.312Z
INSDC last update
SRS9220231
INSDC secondary accession
live
INSDC status
Metagenome or environmental
NCBI submission model
Metagenome.environmental.1.0
NCBI submission package
SRS9220231
SRA accession
2020-07-01
collection_date
Stool sample from healthy donor enriched with RBD binding antibodies.
description
Fresh stool samples were resuspended at 100 µg/mL, filtered for 30 µm and stored in glycerol medium at -80 °C. Frozen stocks were thawed and stained with anti-human IgA1 and anti-human IgA2 antibodies. Sorting of IgA bound events was done using at a BD Influx™ cell sorter. The sorted bacteria were washed and directly subjected to the Illumina Nextera® XT workflow using the primers 5´-TCgTCggCAgCgTCAgATgTgTATAAgAgACAgCCTACgggNggCWgCAg-3’ and 5´-gTCTCgTgggCTCggAgATgTgTATAAgAgACAggACTACHVgggTATCTAATCC-3’ for the first amplicon PCR. After Nextera® v2 index PCR and quality control by capillary gel electrophoresis samples were pooled and sequenced on MiSeq™ device (Illumina).
design_description
HC-019
donor
Germany: Berlin
geo_loc_name
Homo sapiens
host
HEALTHY
host diseace
human_stool
isolation_source
52.52 N 13.37 E
lat_lon
human gut metagenome
http://purl.obolibrary.org/obo/NCBITaxon_408170
organism
Surek@drfz.de durek IgA : hup
title