SAMN36697032
External Id
Montana State University
INSDC center name
2023-09-01T06:42:03.863Z
INSDC first public
2023-09-01T06:42:03.863Z
INSDC last update
SRS18418469
INSDC secondary accession
live
INSDC status
SARS-CoV-2: clinical or host-associated
NCBI submission model
SARS-CoV-2.cl.1.0
NCBI submission package
SRS18418469
SRA accession
BEI Resources
biomaterial_provider
missing
collected_by
2020-01-19
collection_date
In vitro transcribed RNA of SARS-CoV-2 N-gene was synthesized with MEGAscript T7 (Thermo Fisher Scientific) using PCR product generated from SARS-CoV-2 WA1 cDNA and primers N0.5_F TAATACGACTCACTATAGGGCGTGTTGTTTTAGATTTCATC N3-5_R CACACTGATTAAAGATTGCTATGTG The resulting IVT RNA was cleaved with type III-A CRISPR complex of Streptococcus thermophilus and then ligated with T4 RNA ligase to introduce a programmable deletion of 18 or 24 nucleotides.
description
USA: Washington, Snohomish County
geo_loc_name
missing
host
missing
host_disease
USA-WA1/2020
isolate
Oropharyngeal swab
isolation_source
VeroE6
lab_host
Severe acute respiratory syndrome coronavirus 2
http://purl.obolibrary.org/obo/NCBITaxon_2697049
organism
In vitro transcribed RNA of SARS-CoV-2 N gene
title