SAMN37042398
External Id
Pacific Northwest National Laboratroy
INSDC center name
2023-09-07T00:00:00Z
INSDC first public
2023-09-07T16:20:03.196Z
INSDC last update
SRS18647261
INSDC secondary accession
live
INSDC status
MIMS.me
NCBI submission model
MIGS/MIMS/MIMARKS.soil
NCBI submission model
MIMS.me.soil.6.0
NCBI submission package
SRS18647261
SRA accession
2020-06
collection_date
0-20cm
depth
Surface soils (0-20 cm deep, 5 cm diameter cores) were collected from 16 randomly selected locations immediately adjacent to the Tall Wheatgrass Irrigation Field Trial experiment in Prosser, WA. Samples were transported to the Pacific Northwest National Laboratory (PNNL) on ice and stored at 4℃ until processing. Soils were homogenized on ethanol-sterilized 4 mm sieves, and rocks were removed. Sixty grams (dry weight soil equivalent) were weighed into autoclaved 4 oz jars, and the soils were brought to 75% water holding capacity by addition of sterile deionized water. Three replicate incubations were set up for each of the two sampling time points (n=6). First, the soils were preincubated for one week during which time they were gradually exposed to increasing temperature until the target incubation temperature of 30C average summer temperature was reached. The incubation temperatures were: 10 C on day 1, 15C on day 3, 25C on day 5 and 30C on day 7. During the pre-incubation water was added to the soils daily to maintain them at 75% water holding capacity by mass. Once at 30C, three samples were harvested representing the pre-desiccation soils. Sample was stored at -80C until being processed for DNA and RNA extractions.
description
250m
elev
ENVO:01000177 (grassland biome)
env_broad_scale
ENVO:00005750 (grassland soil)
env_local_scale
ENVO:00001998 (soil)
env_medium
USA: Washington, Prosser, Irrigated Agricultural Research and Extension Center (IAREC)
geo_loc_name
46.25 N 119.73 W
lat_lon
soil metagenome
http://purl.obolibrary.org/obo/NCBITaxon_410658
organism
1
replicate
4mm
sieving
aridisol
soil_type
75% WHC rep 1
title
75% WHC
water_content
gravimetric
water_content_soil_meth