SAMN37220994
External Id
Pacific Northwest National Laboratroy
INSDC center name
2023-09-07T00:00:00Z
INSDC first public
2023-09-07T16:20:03.036Z
INSDC last update
SRS18806323
INSDC secondary accession
live
INSDC status
MIMS.me
NCBI submission model
MIGS/MIMS/MIMARKS.soil
NCBI submission model
MIMS.me.soil.6.0
NCBI submission package
SRS18806323
SRA accession
2020-08-19
collection_date
0-20cm
depth
Surface soils (0-20 cm deep, 5 cm diameter cores) were collected from 16 randomly selected locations immediately adjacent to the Tall Wheatgrass Irrigation Field Trial experiment in Prosser, WA. Samples were transported to the Pacific Northwest National Laboratory (PNNL) on ice and stored at 4 until processing. Soils were homogenized on ethanol-sterilized 4 mm sieves, and rocks were removed. Sixty grams (dry weight soil equivalent) were weighed into autoclaved 4 oz jars, and the soils were brought to 75% water holding capacity by addition of sterile deionized water. Three replicate incubations were set up for each of the two sampling time points (n=6). First, the soils were preincubated for one week during which time they were gradually exposed to increasing temperature until the target incubation temperature of 30C average summer temperature was reached. The incubation temperatures were: 10 C on day 1, 15C on day 3, 25C on day 5 and 30C on day 7. During the pre-incubation water was added to the soils daily to maintain them at 75% water holding capacity by mass. Once at 30C, three incubation jars continued to incubate at 30C, without any further moisture additions. Moisture loss was monitored by mass, until complete desiccation was achieved at 9 days after drying commenced. Three replicate samples were harvested at the indicated timepoints. Wet-up treatments were performed at 0 and 7 days after dessication was achieved (days 9 and 16 after drying commenced). Water was added to 75% WHC and samples were taken after 10, 30 and 90 minutes. Sample was stored at -80C until being processed for DNA and RNA extractions.
description
250m
elev
ENVO:01000177 (grassland biome)
env_broad_scale
ENVO:00005750 (grassland soil)
env_local_scale
ENVO:00001998 (soil)
env_medium
USA: Prosser, Washington; Washington State University -Irrigated Agriculture Research and Extension Center
geo_loc_name
46.251649 N 119.728770 W
lat_lon
soil metagenome
http://purl.obolibrary.org/obo/NCBITaxon_410658
organism
3
replicate
4mm
sieving
aridisol
soil_type
Drought day 9 Wet 0min rep 3
title
drought day 9 wet 0 min
treatment
75% WHC
water_content
gravimetric
water_content_soil_meth