SAMN37183463
External Id
NIEHS Paul Doetsch Group
INSDC center name
2023-09-15T00:50:29.110Z
INSDC first public
2023-09-15T00:50:29.110Z
INSDC last update
SRS18732525
INSDC secondary accession
live
INSDC status
Microbe, viral or environmental
NCBI submission model
Microbe.1.0
NCBI submission package
SRS18732525
SRA accession
2020-09-28
collection_date
MPAO1 cells derived from sample P1-8C were seeded from a frozen stock in 5 ml of LB media containing 2 ug/ml ciprofloxacion and grown at 37C until saturation. Cells were collected and DNA was extracted using a Maxwell® RSC Cultured Cells DNA Kit (Promega) and its quality determined in an Agilent 5200 Fragment Analyzer System using a Genomic DNA 50kb Kit (Agilent). Indexed pair-end libraries were prepared with TruSeq DNA Library Prep Kit (Illumina Inc, USA) and sequenced on a NextSeq 550 System Mid-Output Flowcell (Illumina Inc, USA) in a NextSeq 500 System Whole-Genome Sequencing Solution (Illumina Inc, USA). The resulting paired-end reads were 150 bp long.
description
USA: Durham, NC
geo_loc_name
not applicable
host
not applicable
isolation_source
Pseudomonas aeruginosa
http://purl.obolibrary.org/obo/NCBITaxon_287
organism
bio_rep17
replicate
cell culture
sample_type
MPA01
strain
WGS of Sample P1-8C MPA01 cells treated with Ciprofloxacin
title