SAMN37408513
External Id
Northwestern University Feinberg School of Medicine
INSDC center name
2023-09-15T00:00:00Z
INSDC first public
2023-09-15T11:01:11.860Z
INSDC last update
SRS18888453
INSDC secondary accession
live
INSDC status
SARS-CoV-2: clinical or host-associated
NCBI submission model
SARS-CoV-2.cl.1.0
NCBI submission package
SRS18888453
SRA accession
Laboratory of Justin Richner at the University of Illinois at Chicago
collected_by
2021-02-26
collection_date
Whole tissue dissection
collection_method
Mice were vaccinated 3 weeks prior to SARS-CoV-2 challenge
date_of_sars_cov_2_vaccination
K18-hACE2 mice were intramuscularly vaccinated with adenovirus type-5 (Ad5) vector vaccines that encoded either the SARS-CoV-2 S open reading frame (Ad5-S) or the N open reading frame (Ad5-N) from SARS-CoV-2/human/USA/USA-WA1/2020. Mice were vaccinated with either 1x10^9 PFU of Ad5-S, Ad5-N, both, or with PBS as a control (n = 5 mice per condition). After three weeks, mice were challenged intranasally with 5x10^4 plaque forming units (PFU) of SARS-CoV-2/human/USA/USA-WA1/2020 and euthanized at 5-days post infection. Whole lung and brain tissue was dissected and RNA extracted for viral whole genome sequencing.
description
K18-hACE2 (B6.Cg-Tg(K18-ACE2)2Prlmn/J) (Jax Strain # 034860) transgenic mice were challenged intranasally with 5*10^4 plaque-forming units (PFU) of SARS-CoV-2/human/USA/USA-WA1/2020 and tissue was harvested at 5 days post-infection.
exposure_event
USA: Illinois, Chicago
geo_loc_name
Homo sapiens
host
brain
host_anatomical_part
COVID-19
host_disease
SARS-CoV-2/human/USA/USA-WA1/2020
isolate
Obtained from BEI Resources (NR-52281)
isolation_source
Severe acute respiratory syndrome coronavirus 2
http://purl.obolibrary.org/obo/NCBITaxon_2697049
organism
Virus was propagated on Vero-E6 cells (ATCC) for one passage prior to use.
passage_method
1
passage_number
yes - completed
prior_sars_cov_2_vaccination
N (orf9)
sars_cov_2_diag_gene_name_1
7.54
sars_cov_2_diag_pcr_ct_value_1
Center for Pathogen Genomics and Microbial Evolution (CPGME) at Northwestern University, Chicago, Illinois
sequenced_by
Ad5-N_m3_brain
title
Mice were immunized intramuscularly (50 μL per quadriceps) with an Ad5 vector expressing SARS-CoV-2 nucleocapsid protein (Ad5-N) diluted in sterile PBS, at 10^9 PFU per mouse.
vaccine_received