SAMN37408545
External Id
Northwestern University Feinberg School of Medicine
INSDC center name
2023-09-15T00:00:00Z
INSDC first public
2023-09-15T11:01:14.357Z
INSDC last update
SRS18888458
INSDC secondary accession
live
INSDC status
SARS-CoV-2: clinical or host-associated
NCBI submission model
SARS-CoV-2.cl.1.0
NCBI submission package
SRS18888458
SRA accession
Laboratory of Justin Richner at the University of Illinois at Chicago
collected_by
2022-08-23
collection_date
Whole tissue dissection
collection_method
none
date_of_sars_cov_2_vaccination
K18-ACE2 mice were challenged intracranially with 1x10^2 plaque forming units (PFU) of SARS-CoV-2/human/USA/USA-WA1/2020 and euthanized at 3-days post infection. Whole lung and brain tissue was dissected and RNA extracted for viral whole genome sequencing.
description
K18-hACE2 (B6.Cg-Tg(K18-ACE2)2Prlmn/J) (Jax Strain # 034860) transgenic mice were challenged intracranially with 1*10^2 plaque-forming units (PFU) of SARS-CoV-2/human/USA/USA-WA1/2020 and tissue was harvested at 3 days post-infection.
exposure_event
USA: Illinois, Chicago
geo_loc_name
Homo sapiens
host
lung
host_anatomical_part
COVID-19
host_disease
SARS-CoV-2/human/USA/USA-WA1/2020
isolate
Obtained from BEI Resources (NR-52281)
isolation_source
Severe acute respiratory syndrome coronavirus 2
http://purl.obolibrary.org/obo/NCBITaxon_2697049
organism
Virus was propagated on Vero-E6 cells (ATCC) for one passage prior to use.
passage_method
1
passage_number
no
prior_sars_cov_2_vaccination
N (orf9)
sars_cov_2_diag_gene_name_1
24.468
sars_cov_2_diag_pcr_ct_value_1
Center for Pathogen Genomics and Microbial Evolution (CPGME) at Northwestern University, Chicago, Illinois
sequenced_by
WT_intracranial_m5_lung
title
None.
vaccine_received