SAMN37279562
External Id
University of Science and Technology of China
INSDC center name
2023-10-31T00:50:56.820Z
INSDC first public
2023-10-31T00:50:56.820Z
INSDC last update
live
INSDC status
Invertebrate
NCBI submission model
Invertebrate.1.0
NCBI submission package
ERS17017157
SRA accession
6-10 days
age
w1118
breed
2020-10-01
collection_date
Three independent biological replicates of 10 adult w1118 flies under the indicated conditions were washed by PBS, and immersed in Trizol Reagent (Invitrogen), and the subsequent RNA extraction and sequencing were completed by GENEWIZ Inc. RNA was extracted following the manufacturer instructions. The quality and integrity of RNA were evaluated by RNA 6000 Pico Kit (Agilent) and Nanodrop 2000 (Thermo Fisher Scientific). Libraries with different indexes were prepared and loaded on an Illumina HiSeq/ Illumina Novaseq/ MGI2000 instrument for sequencing using a 2X150 paired-end (PE) configuration according to the manuals. RNA-seq data of gene expression profile changes were analyzed by the DESeq2 Bioconductor package, a model based on the negative binomial distribution.
description
Adult
dev_stage
China
geo_loc_name
lab
isolation_source
Drosophila melanogaster
http://purl.obolibrary.org/obo/NCBITaxon_7227
organism
female
sex
25
temp
whole body
tissue
Invertebrate sample from Drosophila melanogaster
title