SAMN37578188
External Id
USDA-ARS-ETSARC
INSDC center name
2023-09-28T00:00:00Z
INSDC first public
2023-09-29T10:00:26.977Z
INSDC last update
SRS19011248
INSDC secondary accession
live
INSDC status
Plant
NCBI submission model
Plant.1.0
NCBI submission package
SRS19011248
SRA accession
3 months
age
David Horvath
biomaterial_provider
Laura Kelley
collected_by
2022-06-22
collection_date
commercial hybrid
cultivar
corn was planted with four individual weeds (canola) surrounding a corn seed in the center of an 8 pot. The weeds were planted in conetainers that penetrated through the bottom of the pot so that there was no chance for direct root-to-root contact between the corn and the canola. No opaque plastic cone-shaped barrier were used, and thus weed-generated light quality (increased far-red light due to reflection from the canola) signal could impact the corn seedling. The plants were grown under greenhouse conditions with diffuse light and an 18 Hr photoperiod at about 25 C for 3 months (until the plants were at the V12 stage of growth). Plants were watered daily and fertilized weekly by additional watering with 20:20:20. Plant root (including roughly equal amount by weight of support roots and horizontal roots) and leaf tips (the distal 10 cm of the top-most fully expanded leaf) were harvested separately into liquid nitrogen for future RNA extractions.
description
V12
dev_stage
NA
ecotype
USA: Fargo, ND
geo_loc_name
84 day
isolate
Zea mays
http://purl.obolibrary.org/obo/NCBITaxon_4577
organism
20-25C
temp
distal 10 cm of top-most fully expanded leaf
tissue
experiment 1 corn leaf tissue where only the below-ground weed-generated signals were blocked
title
weedy with only below-ground barriers
treatment