SAMN37578184
External Id
USDA-ARS-ETSARC
INSDC center name
2023-09-28T00:00:00Z
INSDC first public
2023-09-29T10:00:27.143Z
INSDC last update
SRS19011249
INSDC secondary accession
live
INSDC status
Plant
NCBI submission model
Plant.1.0
NCBI submission package
SRS19011249
SRA accession
3 months
age
David Horvath
biomaterial_provider
Laura Kelley
collected_by
2022-06-22
collection_date
commercial hybrid
cultivar
corn was planted with four individual weeds (canola) surrounding a corn seed in the center of an 8 pot. The weeds were planted outside conetainers so that there was direct root-to-root contact between the corn and the canola. To block the above-ground weed-generated signals, an opaque plastic cone-shaped barrier was placed, point down, over the corn seedling to prevent any weed-generated light quality (increased far-red light due to reflection from the canola) signal from impacting the corn seedling. This cone was enlarged as the corn and canola grew so that any above-ground light quality signal was blocked until after the corn had reached the V8 stage of growth. The plants were grown under greenhouse conditions with diffuse light and an 18 Hr photoperiod at about 25 C for 3 months (until the plants were at the V12 stage of growth). Plants were watered daily and fertilized weekly by additional watering with 20:20:20. Plant root (including roughly equal amount by weight of support roots and horizontal roots) and leaf tips (the distal 10 cm of the top-most fully expanded leaf) were harvested separately into liquid nitrogen for future RNA extractions.
description
V12
dev_stage
NA
ecotype
USA: Fargo, ND
geo_loc_name
84 day
isolate
Zea mays
http://purl.obolibrary.org/obo/NCBITaxon_4577
organism
20-25C
temp
mix or lateral and support roots
tissue
experiment 1 corn root tissue where only the above-ground weed-generated signals were blocked
title
weedy with only above-ground barriers
treatment