SAMN37714360
External Id
Tongji medical college, huazhong university of science and technology
INSDC center name
2023-10-21T00:00:00Z
INSDC first public
2023-10-22T20:17:12.773Z
INSDC last update
SRS19224675
INSDC secondary accession
live
INSDC status
Model organism or animal
NCBI submission model
Model.organism.animal.1.0
NCBI submission package
SRS19224675
SRA accession
P18
age
2023-07-15
collection_date
METTL16 is reported recently to catalyze the formation of N6-methyl-adenosine (m6A) which involves in regulating several biological processes. However, the role of Mettl16 in male germ cells remains elusive. Herein, we identify the essential functions for METTL16 in meiotic prophase I using an inducible germline-specific inactivation strategy. METTL16 deficiency causes failed DDR proteins expansion to chromatin-wide region among sex chromosomes, inter-sister chromatids abnormal synapsis, and delayed DSB formation. Depletion of METTL16 results in an enormous deregulation of transcriptome in pachytene spermatocytes, and 80% of differential genes on sex chromosomes undergo upregulation. METTL16 cooperates with FUS/EWSR1 to regulate DSB formation in early meiotic prophase I, and also interacts with H2AX/MDC1/SCML2/MacroH2A1 which are closely related to MSCI. Furthermore, METTL16 interacts with translational initiation factor eIF3B in spermatocytes. Using MeRIP-seq, we found decreased m6A level in RNAs of some genes related to meiosis process like Ankrd31, Mdc1, Smc3, Rpa2 etc. Our results demonstrate that the involvement of METTL16 in meiosis progression is through cooperating with translational complex and modulating m6A modification on some specific genes, which creatively reveal the indispensable roles of methyltransferase METTL16 in meiosis progression.
description
juvenile
dev_stage
China
geo_loc_name
Mus musculus
http://purl.obolibrary.org/obo/NCBITaxon_10090
organism
male
sex
C57BL/6
strain
testis
tissue
spermatocytes in Mus musculus for m6a
title