SAMN37759876
External Id
RIKEN
INSDC center name
2023-10-11T00:00:00Z
INSDC first public
2023-10-12T07:09:19.667Z
INSDC last update
SRS19137771
INSDC secondary accession
live
INSDC status
Human
NCBI submission model
Human.1.0
NCBI submission package
SRS19137771
SRA accession
not applicable
age
RIKEN BRC
biomaterial_provider
HeLa
cell_line
not applicable
collection_date
Protocols: HeLa (RCB0007, RIKEN BRC) cells were maintained in Dulbecco’s Modified Eagle Medium (Nacalai Tesque) containing 10% fetal bovine serum (gibco) and 1% penicillin-streptomycin (Sigma-Aldrich). Each cell was stained by CellTrace (Invitrogen™) and transfected with barcoded DNA tags by lipofection (4 h incubation with 20 nM DNA tag) or electroporation (250 V, capacitance: 2000 μF, resistance: ∞ Ohm, duration: 20 ms with 100 nM DNA tag). Cells were cultured for varying durations up to 48 h. mRNA and DNA tags were collected by colour-coded hydrogel beads from the cell pool in microwells. RNA-derived and DNA tag-derived cDNAs were fractionated with SPRIselect beads (B23318, Beckman Coulter) after PCR amplification.
description
cervical carcinoma
disease
not applicable
geo_loc_name
Mixture of mRNA from the HeLa cells obtained by Opto-combinatirial indexing
isolate
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
organism
RNA and DNA tag
sample_type
female
sex
cervix
tissue
Opto-combinatorial indexing, Tag retention experiment
title