SAMN37733350
External Id
Loyola University Chicago
INSDC center name
2023-10-09T00:00:00Z
INSDC first public
2023-10-09T12:50:24.700Z
INSDC last update
SRS19104947
INSDC secondary accession
live
INSDC status
MIGS/MIMS/MIMARKS.human-associated
NCBI submission model
MIMARKS.specimen
NCBI submission model
MIMARKS.specimen.human-associated.6.0
NCBI submission package
SRS19104947
SRA accession
Not applicable
collection_date
To create the mock communities, five urinary strains of E. coli, one urinary strain of P. mirabilis, one urinary strain of S. epidermidis, and one urinary strain of E. faecalis were used. The E. coli strains represented different E. coli phylotypes, with two representatives from the B2 group. Each isolate was grown in their respective media; DNA was extracted using the Qiagen Blood & Tissue kit. 400, 50, 300, 100, and 50 ng of DNA for E. coli strains UMB1180, UMB1162, UMB1225, UMB0103, and UMB1220 was combined, respectively. Additionally 100, 120, and 54 ng of E. faecalis, P. mirabilis, and S. epidermidis DNA was added, respectively.
description
Not applicable
env_broad_scale
Not applicable
env_local_scale
Not applicable
env_medium
Not applicable
geo_loc_name
Homo sapiens
host
26962083
isol_growth_condt
Not applicable
lat_lon
urine metagenome
http://purl.obolibrary.org/obo/NCBITaxon_1346744
organism
Mock community #2 containing 5 urinary E. coli, 1 urinary E. faecalis, 1 urinary P. mirabilis, and 1 urinary S. epidermidis strain
strain
Mock community #2 of urinary microbes
title