SAMN37846933
External Id
USGS Eastern Ecological Science Center
INSDC center name
2023-10-16T00:00:00Z
INSDC first public
2023-10-17T10:16:35.040Z
INSDC last update
SRS19176472
INSDC secondary accession
live
INSDC status
MIMS.me
NCBI submission model
MIGS/MIMS/MIMARKS.water
NCBI submission model
MIMS.me.water.6.0
NCBI submission package
Sedna
Niskin_name
SRS19176472
SRA accession
2019-04-17
collection_date
516m
depth
Keywords: GSC:MIxS;MIMS:6.0
description
oceanic epipelagic zone biome
env_broad_scale
oceanic epipelagic zone biome
env_local_scale
sea water
env_medium
Prefilter
filter_material
0.8
filter_pore_size
USA: Atlantic Coast
geo_loc_name
31.76121 N 79.19059 W
lat_lon
Prefilters were extracted using the Qiagen PowerSterivex kit with some modifications. One quarter of the prefilter was lysed in a 5 mL screwcap tube, which mimicked a Sterivex capsule. The rest of the manufacturues protocol was followed, and DNA was eluted with the addition of 50 ul elution buffer.
nucl_acid_ext
metagenome
http://purl.obolibrary.org/obo/NCBITaxon_256318
organism
FWD(1391):5_GTACACACCGCCCGTC, REV(EukBr):5_TGATCCTTCTGCAGGTTCACCTAC
pcr_primers
Illumina MiSeq with v3 chemistry generating paired end 300 bp reads
seq_meth
18S SSU rRNA
target_gene
RB1903_J2_1130_Sedna_18S_10L_Prefilter_run2
title