SAMN38039091
External Id
Department of Biotechnology, University of Hyderabad
INSDC center name
2023-10-30T00:00:00Z
INSDC first public
2023-10-31T23:56:22.040Z
INSDC last update
SRS19374786
INSDC secondary accession
live
INSDC status
Model organism or animal
NCBI submission model
Model.organism.animal.1.0
NCBI submission package
SRS19374786
SRA accession
8-10 weeks old
age
inbreed
breed
2022-12-16
collection_date
This study is based on Parkinson's disease. We used an established and current technique from the literature to induce Parkinson's disease in rats (PMID: 19385059). We employed rotenone as an inducing drug combined with oil to form spheres and shield the medication from early degradation. Rotenone is a pesticide that reduces ATP generation by inhibiting Complex I. After completing the model, we isolate blood through the retro-orbital sinus after ten days of treatment. We separate the serum according to lab protocol before sending it to the firm for sequencing. They isolated small non-coding RNAs and constructed the library with a commercially available kit. Finally, they sequenced the miRNAs using the Illumina platform with a single read length of 50 pb
description
India: Telangana state, Hyderabad
geo_loc_name
monoisolate
isolate
Rattus norvegicus
http://purl.obolibrary.org/obo/NCBITaxon_10116
organism
male
sex
Wistar
strain
whole boold
tissue
Model organism or animal sample from Rattus norvegicus
title