80_S17
Metagenome or environmental
BioSampleModel
2023-11-16T01:09:08Z
ENA-FIRST-PUBLIC
2023-11-16T01:09:08Z
ENA-LAST-UPDATE
SAMN38196827
External Id
pda|rammy1975
INSDC center name
2023-11-16T01:09:08Z
INSDC first public
2023-11-16T01:09:08Z
INSDC last update
public
INSDC status
SRS19542252
SRA accession
80_S17
Submitter Id
NCBI
broker name
2019-10/2021-04
collection_date
bacterial endophytes from lettuce tissues
description
The hypervariable V4 region of 16S rRNA library was constructed by following Illumina MiSeq protocol. Briefly, the protocol included a first stage “amplicon PCR” step involving 0.2 µM concentrations of primers 341F (forward) and 805R (reverse). Sequences were demultiplexed and depleted of Illumina barcodes and primers by using MiSeq reporter and Trimmomatic software
design_description
80_S17_L001_R1_001.fastq.gz
filename
80_S17_L001_R2_001.fastq.gz
filename2
fastq
filetype
South Africa: North West
geo_loc_name
vegetable
host
Illumina MiSeq
instrument_model
lettuce_leaf
isolation_source
26.11 S 28.3 E
lat_lon
80_S17
library_ID
paired
library_layout
PCR
library_selection
METAGENOMIC
library_source
AMPLICON
library_strategy
plant metagenome
organism
ILLUMINA
platform
not applicable
ref_biomaterial
not applicable
rel_to_oxygen
not applicable
samp_collect_device
not applicable
samp_mat_process
not applicable
samp_size
plant metagenome
scientific_name
not applicable
source_material_id
MiSeq of vegetable endophytic bacterial communities: vegetable farms
title