caf1a-1 caf1b-3 ddm1 #9
Plant
BioSampleModel
2023-11-16T01:06:55Z
ENA-FIRST-PUBLIC
2023-11-16T01:06:55Z
ENA-LAST-UPDATE
SAMN33559299
External Id
pda|wingfly1234
INSDC center name
2023-11-16T01:06:55Z
INSDC first public
2023-11-16T01:06:55Z
INSDC last update
public
INSDC status
SRS16928727
SRA accession
caf1a-1 caf1b-3 ddm1 #9
Submitter Id
30-days-old
age
NCBI
broker name
thale cress
common name
All Arabidopsis thaliana materials used in this study were in the Columbia (Col-0) background. The ccr4a-1 (AT3G58560, SAIL_802_A10), ccr4b-1 (AT3G58580, SALK_151541C), caf1a-1 (SALK_070336), and caf1b-3 (SALK_044043) mutants were obtained from the Arabidopsis Biological Resources Center (https://abrc.osu.edu/). The caf1a-1 caf1b-3 double mutant was generated by crossing. De novo DDM1 mutants were generated by CRISPR-Cas9 in the indicated mutant background. Seeds were sterilized using 75% ethanol, sown on Murashige and Skoog (MS) media (0.43 g/L MS salts, 3 g/L sucrose, 0.8% agar, pH 5.8) and stratified at 4 ℃ for 3 days. Plants were grown at 22℃ under long-day conditions (16 h light/ 8 h dark). 10-days-old seedlings in T4 were harvested for both RNA-seq and direct RNA sequencing. 30-days-old seedlings in T6 were harvested for BGI resequencing.
description
Col-0
ecotype
caf1a-1 caf1b-3 ddm1
genotype
not applicable
geo_loc_name
Arabidopsis thaliana
organism
biological replicate 9
replicate
Arabidopsis thaliana
scientific_name
above-ground
tissue
caf1a-1 caf1b-3 ddm1 #9
title