cell_suspension_5
cell_suspension
Biomaterial type
ERS4464103
SRA accession
hindlimb cell suspension
description
enzymatic dissociation
http://www.ebi.ac.uk/efo/EFO_0009128
dissociation_protocol - method
Samples were chopped into a mash using a scalpel and incubated in 5ml of 2.5mg/ml liberase at 37 degrees for 30-40 min till fully digestion. Cells were spun down at 4C 750 for 5min and resuspended in 3ml of 1X RBC lysis buffer (eBioscience 00-4333, or diluted from eBioscience 00-4300) followed by 4-5 min incubation at RT. 10ml of 1X PBS was then added. The cells were then spun down at 4C at 750 for 5min, resuspended in 5ml of 2% FBS in 1X PBS, filtered through a 40um strainer, spun down again iat 4C 750g for 5min, resuspended in 50-200ul of 2% FBS in PBS, and spun down again.
dissociation_protocol - protocol_core - protocol_description
dissociation
dissociation_protocol - protocol_core - protocol_id
Single cell isolation from human fetal thigh
dissociation_protocol - protocol_core - protocol_name
2739
estimated_cell_count
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
organism
Human Cell Atlas
project