387-3-6
Plant
BioSampleModel
2023-11-23T01:14:03Z
ENA-FIRST-PUBLIC
2023-11-23T01:14:03Z
ENA-LAST-UPDATE
SAMN38221123
External Id
pda|ealab
INSDC center name
2023-11-23T01:14:03Z
INSDC first public
2023-11-23T01:14:03Z
INSDC last update
public
INSDC status
SRS19622031
SRA accession
387-3-6
Submitter Id
NCBI
broker name
Zitong Yu
collected_by
2022-05-17
collection_date
bread wheat
common name
Fielder
cultivar
Wheat immunotoxicity is associated with abnormal reaction to gluten-derived peptides. Attempts to reduce immunotoxicity using breeding and biotechnology often affect dough quality. Here, the multiplexed CRISPR-Cas9 editing of cultivar Fielder was used to modify gluten-encoding genes, specifically focusing on omega- and gamma-gliadin gene copies, which were identified to be abundant in immunoreactive peptides based on the analysis of wheat genomes assembled using the long-read sequencing technologies. The whole genome sequencing of an edited line showed mutation or deletion of nearly all omega-gliadin and half of the gamma-gliadin gene copies and confirmed the lack of editing in the alpha/beta-gliadin genes. The estimated 75% and 64% reduction in omega- and gamma-gliadin content, respectively, had no negative impact on the end-use quality characteristics of grain protein and dough. A 47-fold immunoreactivity reduction compared to non-edited line was demonstrated using antibodies against immunotoxic peptides. Our results indicate that the targeted CRISPR-based modification of the omega- and gamma-gliadin gene copies determined to be abundant in immunoreactive peptides by analyzing high-quality genome assemblies is an effective mean for reducing immunotoxicity of wheat cultivars while minimizing the impact of editing on protein quality.
description
2-week seedling
dev_stage
USA:Manhattan
geo_loc_name
387-3-6
isolate
Triticum aestivum
organism
Triticum aestivum
scientific_name
leaf
tissue
Plant sample from genome-edited line 387-3-6 derived from bread wheat cultivar Fielder
title