SAMN38531877
External Id
European Bioinformatics Institute
INSDC center name
2023-12-03T06:41:14.483Z
INSDC first public
2023-12-03T06:41:14.483Z
INSDC last update
SRS19692603
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
SRS19692603
SRA accession
not collected
collection date
Protocols: Bacteria were harvested in ice-cold killing buffer (50 mM Tris pH 7.5, 5 mM MgCl2, 20 mM NaN3), centrifuged at 4750 rpm for 10 min at 4°C and the pellets were immediately frozen in liquid nitrogen and stored at −80°C. S.p. were cultivated in supplemented RPMI medium in the presence or absence of 10% FCS, which was added to the +FCS culture at an OD600 of 0.3. The control1 culture was harvested prior to FCS treatment. The +FCS and –FCS cultures were harvested after 2 hours of growth after reaching OD600 of 0.3. 10% FCS, was added to the +FCS culture at an OD600 of 0.3 for 2 hours. RNA isolation was performed using an acidic phenol-chloroform extraction protocol (Wetzstein et al., 1992). After DNase-I treatment (Zymo Research, Germany), the RNA quality was checked by Trinean Xpose (Gentbrugge, Belgium) and the Agilent RNA Nano 6000 kit using an Agilent 2100 Bioanalyzer (Agilent Technologies, Böblingen, Germany). For RNA-seq transcriptomics, Ribo-Zero rRNA Removal Kit (Bacteria) from Illumina (San Diego, CA, USA) was used to remove the rRNA. TruSeq Stranded mRNA Library Prep Kit from Illumina (San Diego, CA, United States) was applied to prepare the cDNA libraries.
description
not collected
geographic location (country and/or sea)
not applicable
isolate
Streptococcus pneumoniae
http://purl.obolibrary.org/obo/NCBITaxon_1313
organism
Streptococcus pneumoniae
organism
whole organism
organism part
Streptococcus pneumoniae
scientific_name
D39
strain
Sp_FCS2_S8
title