SAMN38531920
External Id
European Bioinformatics Institute
INSDC center name
2023-12-03T06:41:14.500Z
INSDC first public
2023-12-03T06:41:14.500Z
INSDC last update
SRS19692605
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
SRS19692605
SRA accession
36
age
not collected
collection date
human
common name
Protocols: All samples are collected at University Hospital for Infectious Diseases in Zagreb as a part of the study of molecular epidemiology of HIV infection. For this purpose clinical plasma samples were collected from HIV-1 patients. HIV-1 RNA was extracted from 500 μl of patient's plasma sample by using the QIAmp Viral RNA Mini Kit (Qiagen, Hilden, Germany). HIV-1 RNA was reverse transcribed with SuperScript III First-Strand Synthesis System for RT-PCR (Invitrogen, Carlsbad, CA) and HIV-1 specific primer.Amplification of target region for each sample was done in 4 separate multiplex PCR reactions using ALLinTM Taq DNA Polymerase (highQu GmbH, UK). For this purpose we constructed 22 primer pairs that span specific region of interest. After multiplex PCR, amplicons of each sample were pooled in one tube and purified with Agencourt Ampure XP beads (Beckman Coulter, Germany). Viral DNA libraries were prepared for deep sequecing with NEBNext UltraTM II DNA Library Prep Kit for Illumina (MA, USA), according to the manufacturer's instructions.
description
adult
developmental stage
HIV infection
disease
not collected
geographic location (country and/or sea)
not applicable
isolate
HIV-1 Subtype A (A1)
isolate
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
organism
Homo sapiens
organism
blood plasma
organism part
Homo sapiens
scientific_name
H34
title