SAMN27622667
External Id
University of California, San Francisco
INSDC center name
2023-11-29T00:42:34.343Z
INSDC first public
2023-11-29T00:42:34.343Z
INSDC last update
SRS15730026
INSDC secondary accession
live
INSDC status
Model organism or animal
NCBI submission model
Model.organism.animal.1.0
NCBI submission package
SRS15730026
SRA accession
Ophir Klein Lab, UCSF
biomaterial_provider
Bl6
breed
multiple CRISPR-modified and control regional organoid lines
cell_line
intestinal epithelial cell
cell_type
Intestinal organoids derived from the distal-most domain of the small intestine from adult Lgr5creERT2; Rosa26 LSL-Cas9-eGFP/+; ROSA26tdTomato mice, in which cas9-GFP is expressed in a tamoxifen-inducible manner. Sequenced organoids are either experimental Ppard (2 mutants) or Cdx1 (1 mutant) knockout organoids lines, which were transdueced with dual guide RNAs targeting either gene and CRISPR-mediated gene deletion, or control lines (uninfected with virus containing dual guide RNAs with our without 4-hydroxytamoxifen treatment, or infected with dual guide RNAs but untreated with 4-hydroxytamoxifen).
description
culture
dev_stage
regional organoids
isolate
Mus musculus
http://purl.obolibrary.org/obo/NCBITaxon_10090
organism
not applicable
sex
Lgr5creERT2; Rosa26 LSL-Cas9-eGFP/+; ROSA26tdTomato
strain
small intestine
tissue
Regional organoids derived from murine intestine, CRISPR-modified and control lines
title