SAMN31652926
External Id
Shanghai veterinary research institute
INSDC center name
2023-12-01T00:51:24.373Z
INSDC first public
2023-12-01T00:51:24.373Z
INSDC last update
SRS15702354
INSDC secondary accession
live
INSDC status
Model organism or animal
NCBI submission model
Model.organism.animal.1.0
NCBI submission package
SRS15702354
SRA accession
not applicable
age
A genetic stability experiment was performed by stimulating the growth of the vaccine strain in the presence of the wild strain for five passages to test whether the fluorescent reporter gene is lost. To this end, the ASFV-GZdeltaI73R virus was mixed with the same dose of wild strain in equal volume, and then infected primary BMDM cells at a multiple of infection of 0.01. The virus culture was harvested for four more passages in primary BMDM cells. Furthermore, we purified the virus in passage 5 by plaque purification and limiting dilution, followed by nanopore sequencing to evaluate its gene stability.
description
not applicable
isolate
Sus scrofa domesticus
http://purl.obolibrary.org/obo/NCBITaxon_9825
organism
not applicable
sex
ASFV_I73R_P0
strain
domesticus
sub_species
not applicable
tissue
Model organism or animal sample from Sus scrofa domesticus
title