SAMN31936456
External Id
Dr. Senckenberg Institute of Pathology
INSDC center name
2023-12-31T00:52:06.347Z
INSDC first public
2023-12-31T00:52:06.347Z
INSDC last update
SRS15909027
INSDC secondary accession
live
INSDC status
Human
NCBI submission model
Human.1.0
NCBI submission package
SRS15909027
SRA accession
64
age
Dr. Senckenberg Institute of Pathology, Frankfurt am Main; Dr. Petra Joh Forschungshaus; Institute for Virology Frankfurt am Main
biomaterial_provider
All laboratory work was performed according to manufacturers instructions. Representative tumor material of the primary tumor was macrodissected. The cell lines were formalin fixed and paraffin embedded and taken as whole slides. The purification of DNA and RNA from FFPE tissue samples was performed using the Maxwell RSC Instrument (Promega Corporation, Madison, Wisconsin, USA) with the Maxwell RSC FFPE Plus RNA Kit (Promega Corporation, Madison, Wisconsin, USA) and QIAamp DNA FFPE Tissue Kit (Qiagen, Hilden, Germany), respectively. The concentration of DNA and RNA was measured with the Qubit 4 Fluorometer (Invitrogen, Thermo Fisher, Waltham, Massachusetts, USA). For the library preparation 20 ng for the OncomineTM Comprehensive Assay v3 (Thermo Fisher, Waltham, Massachusetts, USA) were used. A 540 chip was applied.
design_description
UKF_PEL.bam
filename
bam
filetype
Ion Torrent S5
instrument_model
human penile carcinoma
isolate
UKF_PEL
library_id
paired
library_layout
PCR
library_selection
GENOMIC
library_source
AMPLICON
library_strategy
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
organism
ION_TORRENT
platform
male
sex
penile sqamous cell carcinoma
tissue
UKF_PEL
title
UKF_PEL
title