SAMN37149696
External Id
University of Vienna
INSDC center name
2023-08-25T00:00:00Z
INSDC first public
2023-08-28T08:10:04.123Z
INSDC last update
live
INSDC status
Viral
NCBI submission model
Virus.1.0
NCBI submission package
ERS17798914
SRA accession
2018-01
collection_date
Giant viruses (belonging to the Nucleocytoviricota) are significant lethality agents of various eukaryotic hosts. Metagenomic studies showed that giant viruses are key players in virtually every environment. However, available giant virus isolates are limited to a minuscule number of protist and algae hosts. Here we report on the first viral isolate replicating in the amoeboflagellate Naegleria. This genus includes the infamous human pathogen Naegleria fowleri, the causative agent of the rare but lethal disease primary amoebic meningoencephalitis. We revealed the structure and infection cycle of Naegleriavirus (NiV), and show its unique adaptations to its Naegleria host by fluorescence in situ hybridization, electron microscopy, genomics, and proteomics. Naegleriavirus is only the fourth isolate of the subfamily Klosneuvirinae, a diverse group of viruses known mainly from metagenomic studies. Like other Klosneuviruses, the genome of Naegleriavirus contains an unusually high amount of translation genes, yet it lacks tRNAs. NiV acquired genes from its Naegleria host, which code for heat shock proteins and apoptosis inhibiting factors, presumably for host interactions. Notably, NiV infection was lethal for all tested Naegleria species, including the human pathogen N. fowleri. This study extends our experimental framework for investigating giant viruses and could represent a first step toward the biological control of Naegleria fowleri.
description
Austria: Klosterneuburg
geo_loc_name
Naegleria
host
Naegleria clarki
isolate
activated sludge
isolation_source
Naegleria clarki
lab_host
Catovirus sp. 'naegleriensis'
http://purl.obolibrary.org/obo/NCBITaxon_3072188
organism
Viral sample from Catovirus naegleriensis
title