SAMN32316636
External Id
Gwangju Institue of Science and Technology
INSDC center name
2023-12-18T00:50:12.227Z
INSDC first public
2023-12-18T00:50:12.227Z
INSDC last update
SRS16347446
INSDC secondary accession
live
INSDC status
Model organism or animal
NCBI submission model
Model.organism.animal.1.0
NCBI submission package
SRS16347446
SRA accession
not provided
age
AML12
cell_line
Hepatocyte
cell_type
Using lipofectamine RNAiMAX Transfection reagent (13778150, Invitrogen) and siRNA (Dharmacon, USA), the Ly6d gene was knocked down in vitro and cultured with Opti-MEM media according to the manufacturers instructions. Opti-MEM media was removed 6 h later, and the media was changed to complete DMEM/F12 media with fetal bovine serum, dexamethasone, and ITS containing fructose.
description
Normal
disease
Mus musculus
http://purl.obolibrary.org/obo/NCBITaxon_10090
organism
biological replicate 1
replicate
Ly6d Knock-Down
sample_type
not provided
sex
not provided
strain
Liver
tissue
Ly6d specifically knock-down and fructose treatment
title
fructose
treatment