Aedes aegypti female midgut blood + rapamycin fed 24h replicate 3
SAMN33059328
External Id
National Institute of Allergy and Infectious Diseases
INSDC center name
2023-11-26T06:48:00.497Z
INSDC first public
2023-11-26T06:48:00.497Z
INSDC last update
SRS16679207
INSDC secondary accession
live
INSDC status
Invertebrate
NCBI submission model
Invertebrate.1.0
NCBI submission package
SRS16679207
SRA accession
missing
breed
2022-07
collection_date
Female Aedes aegypti mosquitoes were artifficially fed on commercial human blood supplemented with 20 uM rapamycin (Rap). Intact midguts (15 per bioological replicate) were collected at 24h after blood feeding and placed directly into Trizol reagent. RNA extraction was performed according to manufacturer`s specifications and analyzed with an Agilent 2100 Bioanalyzer (Agilent, USA). The library was constructed using the NEBNextUltraTM II (Directional) RNA with polyA selection library prep kit and sequencing was performed in an Illumina Novaseq 6000 DNA sequencer.
description
USA
geo_loc_name
not applicable
host
Aedes aegypti red eye
isolate
not applicable
isolation_source
Aedes aegypti
http://purl.obolibrary.org/obo/NCBITaxon_7159
organism
Aedes aegypti female midgut blood + rapamycin fed 24h replicate 3
replicate
midgut
tissue
Aedes aegypti female midgut blood + rapamycin fed 24h replicate 3
title