Plant sample from Stylosanthes scabra cultivar '85/UNEB'
SAMN32769879
External Id
Brazilian Agricultural Research Corporation
INSDC center name
2023-12-31T00:57:57.503Z
INSDC first public
2023-12-31T00:57:57.503Z
INSDC last update
live
INSDC status
Plant
NCBI submission model
Plant.1.0
NCBI submission package
ERS17826278
SRA accession
6 months
age
Active Germplasm Bank of the Universidade do Estado da Bahia - Av, R. Edgar Chastinet, s/n - Sao Geraldo, Juazeiro - BA, CEP: 48900-000
biomaterial_provider
Ana Maria Benko-Iseppon, Natoniel Franklin de Melo, Jose Ribambar Costa Ferreira Neto
collected_by
2021-06-20
collection_date
85/UNEB
cultivar
After six months of cultivation, the genomic DNA was extracted from the young leaves using a modified CATB method. The DNA quality was evaluated using a Qubit fluorometer and agarose gel.
description
Brazil: Petrolina, PE
geo_loc_name
The plants were propagated by stem cuttings at EMBRAPA SEMIARIDO (Petrolina - Pernambuco - Brazil) with 10 cm in length. They were submitted to the application of 1,000 ppm of IBA (indolebutyric acid dissolved in mineral talc) and transplanted into 15 x 8 cm plastic bags (3 seedlings/pot) containing ultisol and vermiculite (3:1) as substrate. The cuttings were kept in a climate-controlled greenhouse (with 50% shade) under irrigation (twice a day). After rooting, the plants were transferred to plastic pots (10L) - containing a substrate comprising sand-ultisol-vermiculite - and maintained in a greenhouse under controlled conditions of temperature (25 C), humidity (60), and 12h/day natural light photoperiod (photosynthetic photon flux density (PPFD) = 1.5 x 103 micromoles m^-2 s^-1 per 12 h/day). At this stage, the plants were watered daily, once a day, at 9 am. After six months of cultivation, the plants were collected.
growth_protocol
60 cm
height_or_length
Caatinga environment
isolation_source
12.951417 S 38.459500 W
lat_lon
Stylosanthes scabra
http://purl.obolibrary.org/obo/NCBITaxon_79078
organism
young leaves
sample_type
34 C
temp
leaves
tissue
Plant sample from Stylosanthes scabra cultivar '85/UNEB'
title