SAMN36418502
External Id
Dermatology Hospital, Southern Medical University
INSDC center name
2023-12-24T00:00:00Z
INSDC first public
2023-12-24T22:42:02.460Z
INSDC last update
SRS18277900
INSDC secondary accession
live
INSDC status
Metagenome or environmental
NCBI submission model
Metagenome.environmental.1.0
NCBI submission package
SRS18277900
SRA accession
2023-04-25
collection_date
Genomic DNA were extraction from HEK293T cells with DNeasy Blood & Tissue Kit (Qiagen, 69504); microbial genomic DNA of Staphylococcus epidermidis, Rosemonas mucosa, Staphylococcus hominis and Staphylococcus aureus were extraction with QIAamp DNA Microbiome Kit (Qiagen, 51704); genomic DNA of other microorganisms were extracted by Rapid Bacterial Genomic DNA Isolation Kit (Sangon Biotech, B518225-0050). All procedures were carried out according to the manufacturer instructions. The high molecular weight DNA (> 10 kb) were enriched through low concentrate agarose gel (0.6%) with TBE buffer (0.5%)(0.5). DNA concentrations were determined using the Qubit dsDNA HS (High Sensitivity) assay kit (ThermoFisher, Q32851); DNA purity was measured by NanoDrop based on the assessment curve shape and the ratio of OD 260/280 and OD 260/230. DNA fragment distribution was determined by Qsep100 (Bioptic) with S3 Cartridge (Kilo Base Cartridge, C105106).
description
China:Guangzhou
geo_loc_name
not collected
host
not collected
isolate
Homo sapiens,Staphylococcus epidermidis, Escherichia coli, Roseomonas mucosa, Pseudomonas aeruginosa, Staphylococcus hominis ,Staphylococcus aureus, Neisseria gonorrhoeae
isolation_source
23.12901 N 113.2668 E
lat_lon
synthetic metagenome
http://purl.obolibrary.org/obo/NCBITaxon_1235509
organism
mock sample
sample_type
microbial:Escherichia coli, Staphylococcus epidermidis, Roseomonas mucosa, Pseudomonas aeruginosa, Staphylococcus hominis , Neisseria gonorrhoeae, Staphylococcus aureus eukaryotic:Homo sapiens
strain
Routine_mock
title