SAMN35978546
External Id
Chinese Academy of Sciences
INSDC center name
2023-12-31T01:05:43.510Z
INSDC first public
2023-12-31T01:05:43.510Z
INSDC last update
SRS18059644
INSDC secondary accession
live
INSDC status
Model organism or animal
NCBI submission model
Model.organism.animal.1.0
NCBI submission package
SRS18059644
SRA accession
4 month old
age
2021-07-20
collection_date
The fish were fasted for 24 h before being transferred into the exposure tank and let to acclimate the new surroundings for 24 h at 28 °C. The water temperature was decreased to 20 °C with a rate of 2 °C/h and maintained for 20 h. After that, the water temperature was further decreased to 12 °C with the same rate and maintained at 12 °C for another 20 h. The fish were sampled at 28 °C, initiation and end of 20 °C (20°Ci and 20°Ce), and initiation and end of 12 °C (12°Ci and 12°Ce). The whole brain collected from each fish was individually subjected to total RNA extraction using TRIzol Reagent (Invitrogen). The sample for Iso-seq (isoform sequencing) was prepared by combing equal amount of total RNA from each sample.
description
Juvenile
dev_stage
China:Wuhan
geo_loc_name
Oreochromis aureus
http://purl.obolibrary.org/obo/NCBITaxon_47969
organism
not collected
sex
not collected
strain
brain
tissue
Blue tilapia Iso-seq data
title