SAMN36467479
External Id
jiangnan university
INSDC center name
2023-12-31T00:54:34.107Z
INSDC first public
2023-12-31T00:54:34.107Z
INSDC last update
SRS18311569
INSDC secondary accession
live
INSDC status
MIGS.ba
NCBI submission model
MIGS.ba.6.0
NCBI submission package
SRS18311569
SRA accession
2022-04-08
collection_date
Keywords: GSC:MIxS;MIGS:6.0
description
missing
env_broad_scale
missing
env_local_scale
missing
env_medium
missing
geo_loc_name
To compare MprA expression from the plasmid in the △mprAB mutant strain and the empty plasmid in the △mprAB mutant strain, 1 mM IPTG was added to the medium at OD600=5.0-6.0. The cultures were harvested after one hour. Cells were washed three times using PBS buffer (137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO4, 1.4 mM KH2PO4), harvested by centrifugation at 4°C, and the pelleted cells were quick-frozen in liquid nitrogen
isol_growth_condt
missing
lat_lon
not collected
num_replicons
Corynebacterium glutamicum
http://purl.obolibrary.org/obo/NCBITaxon_1718
organism
not collected
ref_biomaterial
ATCC13032 overexpressing mprA
strain
MIGS Cultured Bacterial/Archaeal sample from Corynebacterium glutamicum
title