SAMN38565017
External Id
Vanderbilt University Medical Center
INSDC center name
2023-12-08T00:47:15.723Z
INSDC first public
2023-12-08T00:47:15.723Z
INSDC last update
SRS19701898
INSDC secondary accession
live
INSDC status
Model organism or animal
NCBI submission model
Model.organism.animal.1.0
NCBI submission package
SRS19701898
SRA accession
not provided
age
2022-04-14
collection_date
B cells specific to DNA-barcoded envelope glycoproteins of HIV-1 strains were sorted from the splenocytes of two separate guinea pigs. Both guinea pigs were administered with a mix of three strains of HIV-1 Env immunogen (286.36, 5768.04, and KNH1209.18) at three time points, each four weeks apart. Cells were distributed into microfluidic droplets containing unique cell-specific oligonucleotides using the 10X Genomics Chromium Controller. cDNA libraires from single cells were further amplified with a custom mix of BCR enrichment primers specific to guinea pig IgG genes.
description
USA
geo_loc_name
Cavia porcellus
http://purl.obolibrary.org/obo/NCBITaxon_10141
organism
male
sex
Hartley
strain
Spleen
tissue
Model organism or animal sample from Cavia porcellus
title