SAMN38518031
External Id
Vavilov Institute of General Genetics
INSDC center name
2023-12-04T00:00:00Z
INSDC first public
2023-12-05T23:43:25.010Z
INSDC last update
SRS19777018
INSDC secondary accession
live
INSDC status
Plant
NCBI submission model
Plant.1.0
NCBI submission package
SRS19777018
SRA accession
16 days
age
2021-03
collection_date
Two-week-old seedlings were treated with sterile water. A sample of plant tissue for RNA isolation was taken 48 h post water treatment. Total RNA was isolated using Plant RNA Isolation Aid kit (Ambion, ThermoFisher, USA) according to the protocol recommended by the manufacturer. cDNA libraries were obtained with the TruSeq stranded mRNA library Prep kit (Illumina, USA) according to the manufacturer’s protocol. The quality of cDNA libraries was checked with Agilent 2100 Bioanalyzer (Agilent Technologies, USA). Illumina NextSeq500 sequencing was carried out on the equipment of EIMB RAS “Genome” Center.
description
two-three true leaves
dev_stage
Russia: Moscow
geo_loc_name
Control sample, 48 hours postwater treatment, repeat 1
isolate
Filipendula ulmaria
http://purl.obolibrary.org/obo/NCBITaxon_57917
organism
stem and leaves
tissue
3a_S119
title
water (control)
treatment