SAMN38688916
External Id
The First Affiliated Hospital of Zhengzhou University
INSDC center name
2023-12-06T00:00:00Z
INSDC first public
2023-12-06T01:06:17.823Z
INSDC last update
SRS19777659
INSDC secondary accession
live
INSDC status
Metagenome or environmental
NCBI submission model
Metagenome.environmental.1.0
NCBI submission package
SRS19777659
SRA accession
FKDN220505141-1A
SampleID
2021
collection_date
lung transplant event-free (EF)
description
China: Wuxi
geo_loc_name
Homo sapiens
host
gut microbiota fecal samples
isolation_source
31.55 N 120.37 E
lat_lon
metagenome
http://purl.obolibrary.org/obo/NCBITaxon_256318
organism
immediately frozen at -80C. At the beginning of the experiment, each sample weighing 180-200 mg was measured and transferred to a 2 ml centrifuge tube, which was then placed on ice. DNA extraction from the samples was performed using the repeated bead beating plus column (RBB+C) method. In brief, ASL buffer (1.4 ml) was added to each sample, followed by the addition of sterile zirconia beads (0.4 g in total, consisting of 0.3 g of 0.1 mm beads and 0.1 g of 0.5 mm beads). The samples underwent bead beating (3 min, maximum speed) using a Mini-BeadBeater-16 cell disruptor (BioSpec Products, Oklahoma, USA), followed by incubation at 70C for 15 min with thorough shaking every 5 min to facilitate lysis. The subsequent steps of the DNA extraction protocol followed the instructions provided by the manufacturer of the QIAamp Stool Mini Kit (Qiagen, Germany) for bacterial DNA extraction.
samp_mat_process
Metagenome or environmental sample from metagenome
title