SAMN38726487
External Id
Viticultural Research Institute
INSDC center name
2023-12-14T00:00:00Z
INSDC first public
2023-12-14T16:31:19.747Z
INSDC last update
SRS19862208
INSDC secondary accession
live
INSDC status
Invertebrate
NCBI submission model
Invertebrate.1.0
NCBI submission package
SRS19862208
SRA accession
missing
breed
2017-07-25
collection_date
The Illumina standard kit was used for the mRNA-Seq sample preparation, according to the manufacturer's protocol. Briefly, 1,5 g of each total RNA sample was used for polyA mRNA selection using streptavidin-coated magnetic beads, followed by thermal mRNA fragmentation. The fragmented mRNA was subjected to cDNA synthesis using reverse transcriptase (SuperScript II) and random primers. The cDNA was further converted into double stranded cDNA and, after an end repair process (Klenow fragment, T4 polynucleotide kinase and T4 polymerase), was finally ligated to Illumina paired end (PE) adaptors. Size selection was performed using a 2% agarose gel, generating cDNA libraries ranging in size from 200250 bp. Finally, the libraries were enriched using 15 cycles of PCR and purified by the QIAquick PCR purification kit (Qiagen). The enriched libraries were diluted with Elution Buffer to a final concentration of 10 nM. Each library was run at a concentration of 14 pM on one Genome Analyzer (GAII) lane using 36 bp sequencing.
designdescription
DIRENCLI_1.fq.gz
filename
fastq
filetype
Turkey
geo_loc_name
Vitis vinifera
host
Illumina HiSeq 2000
instrument_model
missing
isolate
ManisaTurkey
isolation_source
38.61 N 27.43 E
lat_lon
paired
librarylayout
Lobesia botrana
http://purl.obolibrary.org/obo/NCBITaxon_209534
organism
ILLUMINA
platform
missing
tissue
Codling moth
title