SAMN38704061
External Id
Academia Sinica
INSDC center name
2023-12-07T00:00:00Z
INSDC first public
2023-12-10T11:31:18.887Z
INSDC last update
SRS19824723
INSDC secondary accession
live
INSDC status
Microbe, viral or environmental
NCBI submission model
Microbe.1.0
NCBI submission package
SRS19824723
SRA accession
2021-03-18
collection_date
To identify the causal mutation that led to increased expression noise in the evolved clones, we performed bulk segregant analysis by crossing the evolved cells with their ancestral cells and analyzing their F1 haploid progeny. We measured protein expression noise levels of both reporter genes in 64 F1 segregants and established an ancestral-like pool and an evolved-like pool from each evolved clone (Evo 2, Evo 3 and Evo 4). For each bulk, 20 progenies exhibiting a consistent phenotype across the three replicates were pooled and subjected to whole genome sequencing analysis.
description
Taiwan
geo_loc_name
Laboratory
isolation_source
Saccharomyces cerevisiae
http://purl.obolibrary.org/obo/NCBITaxon_4932
organism
Mix of Genomic DNA
sample_type
W303
strain
Evolved clone 4, evolved-like bulk
title