SAMN38770658
External Id
RIKEN
INSDC center name
2023-12-12T00:00:00Z
INSDC first public
2023-12-12T04:08:22.957Z
INSDC last update
SRS19838084
INSDC secondary accession
live
INSDC status
Human
NCBI submission model
Human.1.0
NCBI submission package
SRS19838084
SRA accession
NA
age
NA
biomaterial_provider
HeLa
cell_line
epithelial cell
cell_type
NA
collection_date
Cell line name : HeLa/Fucci(CA)3.2, a subline of the HeLa cell line expressing Fucci(CA)3.2, a cell cycle marker. Protocols: HeLa/Fucci(CA)3.2 cells were trypsinized, harvested, and sortted into 96-well plates with 100 cells per well containing lysis buffer (Takara) and barcoded oligo-dT primers using a FACSAria (BD Biosciences) into 10 fractions according to Fucci expression. After reverse transcription, cDNA was amplified by PCR and a cDNA library was prepared using Nextera XT DNA Library Preparation Kit (illumina).
description
cervical carcinoma
disease
NA
geo_loc_name
Mixture of mRNA from the HeLa/Fucci(CA)3.2 cell lines fractionated into 10 fractions by FACS according to Fucci expression.
isolate
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
organism
mRNA
sample_type
female
sex
uterus; cervix
tissue
HeLa/Fucci3.2(CCRainbow), 10 fractions sorted according to Fucci
title