SAMN38858543
External Id
Iowa State University
INSDC center name
2023-12-15T00:00:00Z
INSDC first public
2023-12-15T16:01:05.397Z
INSDC last update
SRS19873580
INSDC secondary accession
live
INSDC status
MIGS/MIMS/MIMARKS.water
NCBI submission model
MIMARKS.survey
NCBI submission model
MIMARKS.survey.water.6.0
NCBI submission package
SRS19873580
SRA accession
2021/2023
collection_date
0
depth
Keywords: GSC:MIxS;MIMARKS:6.0
description
500 ml of water filtered onto 3 _m filters. DNA was extracted with the DNeasy Powerbiofilm Isolation kit. DNA was quantified with a Qubit 2.0 Fluorometer. Illumina MiSeq (2x250 bp) was used to sequence the V4 region of the 16S rRNA gene with the primer pair 515F (5-GTGCCAGCMGCCGCGGTAA-3) and 805R (5-GACTACVSGGGTATCTAAT-3) using a dual index approach.
design_description
watershed [ENVO_00000292]
env_broad_scale
stream [ENVO_00000023]
env_local_scale
water surface [ENVO_01001191]
env_medium
CC202322NZ24_S67_L001_R1_001.fastq
filename
CC202322NZ24_S67_L001_R2_001.fastq
filename2
fastq
filetype
USA:Iowa,Ames
geo_loc_name
Illumina MiSeq
instrument_model
42.0319 N 93.6545 W
lat_lon
CC202322NZ24
library_id
paired
library_layout
PCR
library_selection
METAGENOMIC
library_source
AMPLICON
library_strategy
freshwater metagenome
http://purl.obolibrary.org/obo/NCBITaxon_449393
organism
ILLUMINA
platform
16S rRNA metagenome from an intermittent freshwater creek
title
MIMARKS Survey related sample from freshwater metagenome
title