SAMN38867890
External Id
University of Lethbridge
INSDC center name
2023-12-17T00:00:00Z
INSDC first public
2023-12-29T10:42:21.063Z
INSDC last update
SRS20015301
INSDC secondary accession
live
INSDC status
Model organism or animal
NCBI submission model
Model.organism.animal.1.0
NCBI submission package
SRS20015301
SRA accession
21 dpg
age
not applicable
breed
not applicable
collection_date
not applicable
cultivar
gDNA was quantified using the Quant-iT PicoGreen dsDNA Assay Kit (Life Technologies). Libraries were generated using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs) as per the manufacturers recommendations. Adapters and PCR primers were purchased from IDT. Size selection of libraries contained the desired insert size has been performed using SparQ beads (Qiagen). Libraries were quantified using the Kapa Illumina GA with Revised Primers-SYBR Fast Universal kit (Kapa Biosystems). Average size fragment was determined using a LabChip GX (PerkinElmer) instrument.
design_description
Columbia
ecotype
HI.4823.003.BioOHT_91.4_R1.fastq.gz
filename
HI.4823.003.BioOHT_91.4_R2.fastq.gz
filename2
fastq
filetype
not applicable
geo_loc_name
Illumina HiSeq 2000
instrument_model
not applicable
isolate
HI.4823.003.BioOHT_91
library_id
paired
library_layout
RANDOM
library_selection
GENOMIC
library_source
WGS
library_strategy
Arabidopsis thaliana
http://purl.obolibrary.org/obo/NCBITaxon_3702
organism
ILLUMINA
platform
not applicable
sex
Transgenic line 15d8
strain
Leaf
tissue
F25_cd4_WGS
title
Whole genome sequencing of heat exposed Arabidopsis thaliana plants
title
Cold stress
treatment