characteristics.text: SAMEA1468570
characteristics.characteristic: External Id
characteristics.text: UOL_CGR
characteristics.characteristic: INSDC center name
characteristics.text: 2012-06-11T15:10:18Z
characteristics.characteristic: INSDC first public
characteristics.text: 2013-11-04T20:06:04Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS147914
characteristics.characteristic: SRA accession
characteristics.text: Triticum aestivum Avalon variety
characteristics.characteristic: Submitter Id
characteristics.text: bread wheat
characteristics.characteristic: common name
characteristics.text: Genomic DNA was isolated from purified nuclei based on modification of an existing protocol (Peterson et al, Construction of Plant Bacterial Artificial Chromosome (BAC) Libraries: An Illustrated Guide. Second edition, 2002). A modified sucrose buffer SEB (Sucrose-based Extraction Buffer: 10% v/v TKE, 500 mM sucrose, 4 mM spermidine, 1 mM spermine tetrahydrochloride, 1.2g/L PEG 8000, 0.13% w/v sodium diethyldithiocarbamate and 0.2% v/v ß-mercaptoethanol) was used instead of the recommended MEB buffer, which gave poor quality gDNA in wheat. 60-80g frozen leaf material was ground in liquid nitrogen and added to 1 litre SEB, and the protocol followed. Typically 200-500ug of high quality gDNA was extracted using this method.
characteristics.characteristic: description
characteristics.text: Triticum aestivum
characteristics.characteristic: organism
characteristics.text: Triticum aestivum
characteristics.characteristic: scientific_name
characteristics.text: Triticum aestivum, Avalon variety
characteristics.characteristic: title