characteristics.text: 2012-03-01T17:00:25Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2013-11-04T20:04:51Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA1316867
characteristics.characteristic: External Id
characteristics.text: UNIVERSITY OF OSLO
characteristics.characteristic: INSDC center name
characteristics.text: 2012-03-01T17:00:25Z
characteristics.characteristic: INSDC first public
characteristics.text: 2013-11-04T20:04:51Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS026011
characteristics.characteristic: SRA accession
characteristics.text: ATCC 14579
characteristics.characteristic: StrainOrLine
characteristics.text: E-MTAB-450:Bc14579_D_t2.5
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: Protocols: B. cereus strains ATCC 10987 and ATCC 14579 were grown to mid-exponential phase (OD = 0.60) in Luria-Bertani (LB) broth (500 ml) at 30C, 250 rpm. Rifampicin (10 ml) suspended in 5% DMSO/95% LB medium was added to a final concentration of 150 ug/ml for transcriptional arrest. Samples (20 ml) for mRNA isolation were drawn at 0, 2.5, 5, 10 and 20 minutes following Rifampicin addition, immediately suspended in an equal volume of methanol/phenol (50:1), and incubated 3-5 minutes at room temperature, before harvesting of cells by centrifugation (4000 x g, 4C, 5 min). Cell pellets were snap-frozen in liquid nitrogen and stored at -80C. For RNA isolation, cell pellets were suspended in RLT buffer (Qiagen, Germany) and lysed using 0.1 um glass beads in a Precellys 24 instrument at 5800 rpm for 30 sec x 2 (bertin Technologies, France). RNA was isolated using the RNeasy Mini kit (Qiagen, Germany) according to manufacturers protocol, including the optional on-column DNase treatment. Isolated RNA was treated with Turbo DNase (Ambion, US) and subjected to clean-up using the RNeasy Mini kit. Ribosomal RNA (rRNA) was depleted using one (B-series) or two (C and D-series) rounds of treatment with the Microbe Express kit (Ambion, US) following manufacturers instructions.
characteristics.characteristic: description
characteristics.text: Bacillus cereus
characteristics.characteristic: organism
characteristics.text: Bacillus cereus
characteristics.characteristic: organism
characteristics.text: Bacillus cereus
characteristics.characteristic: scientific_name
characteristics.text: Bc14579_D_t2.5
characteristics.characteristic: title