characteristics.text: 2013-09-17T17:00:33Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2013-05-16T10:00:56Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2072027
characteristics.characteristic: External Id
characteristics.text: MRC National Institute for Medical Research
characteristics.characteristic: INSDC center name
characteristics.text: 2013-09-17T17:00:33Z
characteristics.characteristic: INSDC first public
characteristics.text: 2013-05-16T10:00:56Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS246820
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1667:DS_18
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: Protocols: M. tuberculosis H37Rv (ATCC 25618) was grown at 37C in modified Dubos medium (Difco) supplemented with 4% Dubos medium albumin (Difco) and 0.5% w/v glycerol. RNA was prepared using a FastRNA pro blue kit (Qbiogene). Contaminating DNA was removed using a TURBO DNA-free kit (Ambion) 2-3 ug of total RNA were fragmented (Ambion Cat # AM8740) and strand-specific cDNA libraries were constructed using the Illumina directional mRNA-Seq protocol (Part # 15018460 Rev. A) but with exclusion of polyA-tail and size selection to capture all RNA species. Briefly, this protocol ligates the Illumina v1.5 small RNA 3prime adapter followed by a 5prime adapter to preserve strand specificity.
characteristics.characteristic: description
characteristics.text: empty vector
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001461
characteristics.characteristic: genotype
characteristics.text: Mycobacterium tuberculosis
characteristics.characteristic: organism
characteristics.text: Mycobacterium tuberculosis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_1773
characteristics.characteristic: organism
characteristics.text: Mycobacterium tuberculosis
characteristics.characteristic: scientific_name
characteristics.text: fresh specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000971
characteristics.characteristic: specimen with known storage state
characteristics.text: H37Rv
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_83332
characteristics.characteristic: strain
characteristics.text: DS_18
characteristics.characteristic: title