characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-02-01T17:00:47Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2013-12-23T15:04:03Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2293968
characteristics.characteristic: External Id
characteristics.text: Rothamsted Research
characteristics.characteristic: INSDC center name
characteristics.text: 2014-02-01T17:00:47Z
characteristics.characteristic: INSDC first public
characteristics.text: 2013-12-23T15:04:03Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS387085
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2166:third internode
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: Protocols: Axillary buds were isolated from willow stems (Salix purpurea genotype 844: Rothamsted National Willow Collection).Bud scales and stem epidermis removed under a dissecting microscope, and the isolated buds placed upright on plates containing root induction medium. After 4-6 weeks growth at 22-24 degrees C the roots were 1-2cm long and the plants were transferred to root induction medium for another 10 days and then transplanted to soil and moved to a controlled environment cabinet at 20 degrees C /15 degrees C, 14h /10h (day/night) and 330 micromolar m-2 s-1 light. The third stem internodes (counting from the basal stem) were collected at week 0 (before being transplanted to soil) and weeks 2, 4, and 6 (after transplantation to soil) and were either analysed immediately or frozen into liquid nitrogen. In order to obtain sufficient material for analyses, multiple internodes were pooled into samples (~70, ~50, ~20, 8 internodes at 0, 2, 4 and 6 weeks, respectively). manufacturer's protocol for 110 bp SE reads
characteristics.characteristic: description
characteristics.text: Salix purpurea
characteristics.characteristic: organism
characteristics.text: Salix purpurea
characteristics.characteristic: organism
characteristics.text: third internode
characteristics.characteristic: organism part
characteristics.text: Salix purpurea
characteristics.characteristic: scientific_name
characteristics.text: fresh specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000971
characteristics.characteristic: specimen with known storage state
characteristics.text: 4 week
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UO_0000034
characteristics.characteristic: time after transfer to soil
characteristics.text: 0 week
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UO_0000034
characteristics.characteristic: time after transfer to soil
characteristics.text: 2 week
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UO_0000034
characteristics.characteristic: time after transfer to soil
characteristics.text: 6 week
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UO_0000034
characteristics.characteristic: time after transfer to soil
characteristics.text: third internode
characteristics.characteristic: title