characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-12-12T09:24:01Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2014-05-22T13:41:52Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2730179
characteristics.characteristic: External Id
characteristics.text: UQ
characteristics.characteristic: INSDC center name
characteristics.text: 2014-12-12T09:24:01Z
characteristics.characteristic: INSDC first public
characteristics.text: 2014-05-22T13:41:52Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS462730
characteristics.characteristic: SRA accession
characteristics.text: E. coli O157:H7 EDL933 TUV93-0 ∆adhE mutant
characteristics.characteristic: Submitter Id
characteristics.text: Starter cultures were grown overnight at 37˚C in LB and then used to inoculate MEM-HEPES (Sigma) with growth @ 37˚C with shaking (200rpm) until late exponential phase. Total RNA was prepared as described previously (Tree et al., 2009; doi:10.1128/IAI.00562-09) and depleted for ribosomal sequences using a Microbexpress kit (Ambion). Sequencing of cDNA was carried out on an Illumina Genome Analyser IIx using single ended reads and 6 samples per lane.
characteristics.characteristic: description
characteristics.text: Escherichia coli O157:H7
characteristics.characteristic: organism
characteristics.text: Escherichia coli O157:H7
characteristics.characteristic: scientific_name
characteristics.text: EDL933
characteristics.characteristic: strain
characteristics.text: TUV93-0 ∆adhE
characteristics.characteristic: sub_strain
characteristics.text: RNASeq TUV93-0 ∆adhE mutant
characteristics.characteristic: title