characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-01-15T17:01:41Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2014-05-16T17:06:02Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2537123
characteristics.characteristic: External Id
characteristics.text: Shandong Agricultural University
characteristics.characteristic: INSDC center name
characteristics.text: 2015-01-15T17:01:41Z
characteristics.characteristic: INSDC first public
characteristics.text: 2014-05-16T17:06:02Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS460439
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1709:12DAA
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: Protocols: growth protocol: The maize inbred line Q319 was grown in silt loam soil at the Shandong Agricultural University experimental station in Tai'an, China (35'58''N, 116'38''E, 150m above sea level).The upper parts of rod clover leaf (approx. 10-20 cm) were collected at 12 day after anthesis (12DAA), 20 day after anthesis (20DAA) and 28 day after anthesis (28DAA). nucleic acid extraction protocol: Total RNA of each sample was extracted by using the hexadecyl trimethyl ammonium bromide (CTAB) protocol (Gambino et al., 2008) . After extracting the total RNA from the samples, mRNA of the four tissues are enriched by using the oligo(dT) magnetic beads. Adding the fragmentation buffer, the mRNA is interrupted to short fragments (about 200 bp), then the first strand cDNA is synthesized by random hexamer-primer using the mRNA fragments as templates. Buffer, dNTPs, RNase H and DNA polymerase I are added to sythsize the second strand. The double strand cDNA is purified with QiaQuick PCR extraction kit and washed with EB buffer for end repair and single nucleotide A (adenine) addition. Finally, sequencing adaptors are ligated to the fragments. The required fragments is purified by agrose gel electrophoresis and enriched by PCR amplification.
characteristics.characteristic: description
characteristics.text: 12 days post anthesis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0007016
characteristics.characteristic: developmental stage
characteristics.text: Zea mays
characteristics.characteristic: organism
characteristics.text: Zea mays
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4577
characteristics.characteristic: organism
characteristics.text: upper parts of rod clover leaf (approx. 10-20 cm)
characteristics.characteristic: organism part
characteristics.text: Zea mays
characteristics.characteristic: scientific_name
characteristics.text: 12DAA
characteristics.characteristic: title