characteristics.text: 2013-09-12T17:00:57Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2013-11-04T20:06:01Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2007350
characteristics.characteristic: External Id
characteristics.text: Cambridge Systems Biology Centre
characteristics.characteristic: INSDC center name
characteristics.text: 2013-09-12T17:00:57Z
characteristics.characteristic: INSDC first public
characteristics.text: 2013-11-04T20:06:01Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS140640
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1126:T70N_SS_SORB
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: GS 115
characteristics.characteristic: cell line
characteristics.text: Protocols: Derivatives of Pichia pastoris strain GS115 were grown to steady state in chemostat cultures (1L volume in 2L fermenter; 30oC; pH5; 750 rpm stirring; air flow 1L/min) in a defined carbon-limited medium based on sorbitol (20 g/L). Dilution rates at steady state were 0.004-0.015 L/h, and all other non-carbon nutritional requirements were in excess at constant residual concentrations. Heterologous protein synthesis was induced in the strains by changing the carbon source composition of the medium to sorbitol (11.3 g/L) plus methanol (9 g/L). Samples were taken immediately prior to changing to the methanol media, 1 hr and 3 hr after methanol induction and finally after reaching a second steady state in the new media. Cells were lysed mechanically at 4oC in the presence of trizol. After partitioning with chloroform, RNA was precipitated from the aqueous phase using isopropanol. RNA was washed with 70% ethanol, and redissolved in water. 10 microg aliquots of the final RNA solution were ethanol precipitated.
characteristics.characteristic: description
characteristics.text: pPIC9-HuLy_T70N
characteristics.characteristic: genotype
characteristics.text: Komagataella pastoris
characteristics.characteristic: organism
characteristics.text: Pichia pastoris
characteristics.characteristic: organism
characteristics.text: Komagataella pastoris
characteristics.characteristic: scientific_name
characteristics.text: T70N_SS_SORB
characteristics.characteristic: title