characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2013-12-20T17:02:28Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2013-10-24T13:39:01Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2228972
characteristics.characteristic: External Id
characteristics.text: Institute for Molecular Biosciences, Biocenter
characteristics.characteristic: INSDC center name
characteristics.text: 2013-12-20T17:02:28Z
characteristics.characteristic: INSDC first public
characteristics.text: 2013-10-24T13:39:01Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS363126
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2016:day10
characteristics.characteristic: Submitter Id
characteristics.text: 10 day
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: Protocols: For all experiments, three independent monokaryotic spore isolates (mating type minus) of the wild-type strain 's' (Rizet 1953, CR Acad Sci Paris 237: 838-855) were used. Cultivation was essentially performed as described previously (Rexroth et al. 2012 Biochim Biophys Acta 1817: 381-387). Briefly, solid PASM medium (Brust et al. 2010 Curr Genet 55: 225-235), overlaid with a cellophane sheet, was inoculated with small pieces of mycelium derived from a culture of a freshly germinated ascospore. To obtain enough biomass for RNA isolation, after 2 days of incubation under permanent light at 27 degree C the mycelium was transferred to liquid CM medium (Borghouts et al. 2000 Curr Genet 37: 268-275) and incubated at 27 degree C for two days under light and agitation (age in total: 6 days). To obtain different age stages (9, 10, 11, 12, 13, and 14 d, respectively), the cultures were pre-grown to e.g. the age of 5 days for the 9 days sample on solid medium. Small pieces of mycelium from the growth front were transferred to fresh PASM plates overlaid with a cellophane sheet, and incubated under permanent light at 27 degree C for two days. Subsequently, the mycelium was transferred to liquid medium and again cultured for 2 days in liquid medium (permanent light, shaking, 27 degree C). To be sure that all three isolates have a similar aging behaviour, the lifespan as period of linear growth on solid PASM medium was recorded. All isolates had a lifespan of 14 days, thus the oldest age stage (14 days) represents a senescent culture. Total RNA was isolated using a CsCl density gradient as described in Servos et al. 2012 PLoS ONE 7: e49292. SuperSAGE analysis was performed by GenXPro as described in Molina et al. 2008 BMC Genomics 9: 553. Additionally to the anchoring enzyme NlaIII (CATG), DpnII (GATC) had been used.
characteristics.characteristic: description
characteristics.text: wild type
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: Podospora anserina
characteristics.characteristic: organism
characteristics.text: Podospora anserina
characteristics.characteristic: organism
characteristics.text: mycelium
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/BTO_0001436
characteristics.characteristic: organism part
characteristics.text: Podospora anserina
characteristics.characteristic: scientific_name
characteristics.text: mating type minus
characteristics.characteristic: sex
characteristics.text: fresh
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000971
characteristics.characteristic: specimen with known storage state
characteristics.text: 's'
characteristics.characteristic: strain
characteristics.text: day10
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UO_0000033
characteristics.characteristic: title