characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-08-01T17:04:22Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2014-08-07T09:23:00Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2689653
characteristics.characteristic: External Id
characteristics.text: University of Aberdeen
characteristics.characteristic: INSDC center name
characteristics.text: 2015-08-01T17:04:22Z
characteristics.characteristic: INSDC first public
characteristics.text: 2014-08-07T09:23:00Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS523471
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2822:NaCl_60_B
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: Protocols: Candida albicans (strain CAI4-CIp10) was grown in YPD-T (Tris buffered YPD: 2% w/v glucose, 2% w/v mycological peptone, 1% w/v yeast extract, 100 mM Tris.HCl, pH 7.4) to mid-exponential phase Cultures were split and exposed to stresses in fresh medium at an OD600 of 0.2. Biological triplicate cultures were processed After 10 min cells were flash frozen in liquid nitrogen and RNA was extracted using Qiazol (phenol/chloroform method). An unstressed sample (timepoint 0 min) was used as control. RNA was DNase treated using Ambion Turbo DNAfree kit before being sent to GenePool. Libraries were prepared using standard Illumina RNAseq library preparation kit.
characteristics.characteristic: description
characteristics.text: ura3::imm434/ura3::imm434 + CIp10
characteristics.characteristic: genotype
characteristics.text: Candida albicans
characteristics.characteristic: organism
characteristics.text: Candida albicans
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_5476
characteristics.characteristic: organism
characteristics.text: Candida albicans
characteristics.characteristic: scientific_name
characteristics.text: frozen specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000922
characteristics.characteristic: specimen with known storage state
characteristics.text: CAI4-CIp10
characteristics.characteristic: strain
characteristics.text: NaCl_60_B
characteristics.characteristic: title