characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-06-30T17:02:03Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2014-05-06T10:31:52Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2500728
characteristics.characteristic: External Id
characteristics.text: Institut Forel, University of Geneva
characteristics.characteristic: INSDC center name
characteristics.text: 2014-06-30T17:02:03Z
characteristics.characteristic: INSDC first public
characteristics.text: 2014-05-06T10:31:52Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS450609
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2557:GLN-17
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: Protocols: Elodea nuttallii were grown in 25-L aquariums supplemented with a layer of sediment in a 16/8 h light/dark cycle at 20 degrees C. 3 shoots per treatment were cut and exposed in 1-L PE beakers to 0 (control), 20, 200 2000, 20000 or 200000 ng/L HgCl2, 10, 100, 1000 or 10000 ng/L methyl-Hg, or 10-6 M CuCl2 for 24 h, or to 51 uW/cm2 UV-B radiation for 6 h. Double exposures were conducted for UV-B with 200 or 200000 ng/L HgCl2. All treatments except 20 and 20000 ng/L HgCL2, 10, 100 and 1000 ng/L methyl-Hg and 10-6 M Cu were run in biological triplicate. Shoots were ground in liquid nitrogen and RNA was extracted using Trizol reagent. Libraries were constructed at Fasteris SA (Geneva, CH) using the Illumina TruSeq stranded mRNA kit according to the manufacturer's instructions. Briefly, mRNA was purified and fragmented, and subsequently first and second cDNA strands were synthesised. Finally, after adapter ligation, libraries were PCR amplified and quality checked.
characteristics.characteristic: description
characteristics.text: Elodea nuttallii
characteristics.characteristic: organism
characteristics.text: Elodea nuttallii
characteristics.characteristic: organism
characteristics.text: shoot
characteristics.characteristic: organism part
characteristics.text: Elodea nuttallii
characteristics.characteristic: scientific_name
characteristics.text: frozen specimen
characteristics.characteristic: specimen with known storage state
characteristics.text: GLN-17
characteristics.characteristic: title