characteristics.text: 2013-06-27T17:00:22Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2013-01-29T17:30:20Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SRA-Webin
characteristics.characteristic: ENA-SUBMISSION-TOOL
characteristics.text: SAMEA2047073
characteristics.characteristic: External Id
characteristics.text: INRA-CMSE
characteristics.characteristic: INSDC center name
characteristics.text: 2013-06-27T17:00:22Z
characteristics.characteristic: INSDC first public
characteristics.text: 2013-01-29T17:30:20Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: 0.35 + or - 0.11 mg of DNA/g of soil organic carbon
characteristics.characteristic: Molecular Microbial Biomass
characteristics.text: ERS209841
characteristics.characteristic: SRA accession
characteristics.text: GnS_2012_LaosPL_CT_16S
characteristics.characteristic: Submitter Id
characteristics.text: 2010-29-06
characteristics.characteristic: collection date
characteristics.text: Conventional tillage
characteristics.characteristic: current land use
characteristics.text: 0.10
characteristics.unit: m
characteristics.characteristic: depth
characteristics.text: DNA was extracted from soil with a conventional tillage-based (CT) rotational system, based on annual soil ploughing and repetead human interventions (e.g. sowing, manual weeding, and fertilizer application). Bacterial diversity assessment was realized using 16S rDNA PCR amplification, and pyrosequencing.
characteristics.characteristic: description
characteristics.text: Tropical and subtropical grasslands, savannas, and shrubland biome
characteristics.characteristic: environment (biome)
characteristics.text: tropical soil
characteristics.characteristic: environment (feature)
characteristics.text: ferralsol
characteristics.characteristic: environment (material)
characteristics.text: soil
characteristics.characteristic: environmental package
characteristics.text: Laos, Poa village
characteristics.characteristic: geographic location (countryand/orsea,region)
characteristics.text: 1130
characteristics.unit: m
characteristics.characteristic: geographic location (elevation)
characteristics.text: 19.33
characteristics.unit: DD
characteristics.characteristic: geographic location (latitude)
characteristics.text: 102.59
characteristics.unit: DD
characteristics.characteristic: geographic location (longitude)
characteristics.text: metagenome
characteristics.characteristic: investigation type
characteristics.text: CGTACTCAGA
characteristics.characteristic: multiplex identifiers
characteristics.text: soil metagenome
characteristics.characteristic: organism
characteristics.text: initial denaturation:94degC_2.0min;35cycles of denaturation:94degC_0.5min;annealing:52degC_0.5min;elongation:72degC_1.0min;final elongation:72degC_7.0min
characteristics.characteristic: pcr conditions
characteristics.text: 338F:ACTCCTACGGGAGGCAGCAG; 803R:CTACCNGGGTATCTAAT
characteristics.characteristic: pcr primers
characteristics.text: 5.0
characteristics.characteristic: ph
characteristics.text: GnS_2012_LaosPL
characteristics.characteristic: project name
characteristics.text: 2
characteristics.unit: g
characteristics.characteristic: sample weight for DNA extraction
characteristics.text: soil metagenome
characteristics.characteristic: scientific_name
characteristics.text: Pyrosequencing
characteristics.characteristic: sequencing method
characteristics.text: 16S rRNA
characteristics.characteristic: target gene
characteristics.text: Bacterial diversity by 16S rRNA gene pyrosequencing of soil conventional tillage-based (CT) rotational system.
characteristics.characteristic: title
characteristics.text: 1.9
characteristics.unit: g/kg
characteristics.characteristic: total n
characteristics.text: 28.9
characteristics.unit: g/kg
characteristics.characteristic: total organic carbon