characteristics.text: 2013-12-09T17:01:43Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2012-10-25T16:08:44Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2032750
characteristics.characteristic: External Id
characteristics.text: Institute for Molecular Biology and Tumor Research
characteristics.characteristic: INSDC center name
characteristics.text: 2013-12-09T17:01:43Z
characteristics.characteristic: INSDC first public
characteristics.text: 2012-10-25T16:08:44Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS183175
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1300:AB31 1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: Protocols: Ustilago maydis cells were grown at 28 degrees Celscius in CM (complete medium, {Holliday, 1974}) supplemented with 1% glucose (CM-G), and shifted to CM supplemented with 1% arabinose (CM-A) (to induce the b-heterodimer) 8h before harvesting. Total RNA was extracted using Trizol reagent (Invitrogen) according to the manufacturer's instructions. RNA samples were further column purified (RNeasy; Qiagen) and the quality was checked using a Bioanalyzer with an RNA 6000 Nano LabChip kit (Agilent)
characteristics.characteristic: description
characteristics.text: wild type
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: Ustilago maydis
characteristics.characteristic: organism
characteristics.text: Ustilago maydis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_5270
characteristics.characteristic: organism
characteristics.text: Ustilago maydis
characteristics.characteristic: scientific_name
characteristics.text: AB31 1
characteristics.characteristic: title