characteristics.text: Direct sequencing of the unamplified RNA was undertaken by Helic Biosciences, as described in Ozsolak & Milos (2011) doi: 10.1002/wrna.84
characteristics.characteristic: DRS Sequencing
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-08-01T17:01:10Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2014-07-10T21:48:02Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2634509
characteristics.characteristic: External Id
characteristics.text: DUNDEE
characteristics.characteristic: INSDC center name
characteristics.text: 2014-08-01T17:01:10Z
characteristics.characteristic: INSDC first public
characteristics.text: 2014-07-10T21:48:02Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: Dissections were carried out in L15 medium at 4°C. RNA was extracted in Trizol reagent (Invitrogen) by phase separation with chloroform, followed by precipitation with isopropanol and linear acrylamide. The RNA was washed in 70% ethanol, air-dried, resuspended in DEPCwater and frozen in liquid nitrogen. Total RNA was quantified and quality tested using the Agilent RNA assay (Agilent Bioanalyser pico RNA chip) by ARK-Genomics. Samples with a RIN number above 8.0 where selected. Notochord was removed by controlled trypsin digestion that aimed to keep the neural ventral midline.
characteristics.characteristic: Microdissection
characteristics.text: ERS500964
characteristics.characteristic: SRA accession
characteristics.text: DRS_PNT_CHICK3
characteristics.characteristic: Submitter Id
characteristics.text: Neural Tube
characteristics.characteristic: cell_type
characteristics.text: Olivera-Martinez
characteristics.characteristic: collected_by
characteristics.text: chicken
characteristics.characteristic: common name
characteristics.text: DRS sequencing of Pre-Neutal Tube tissue microdissected from single chick embryos.
characteristics.characteristic: description
characteristics.text: HH10
characteristics.characteristic: dev_stage
characteristics.text: Gallus gallus
characteristics.characteristic: organism
characteristics.text: Gallus gallus
characteristics.characteristic: scientific_name
characteristics.text: Male
characteristics.characteristic: sex
characteristics.text: Pre-neural Tube
characteristics.characteristic: tissue_type
characteristics.text: PNT DRS Individual 3
characteristics.characteristic: title