characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2013-10-08T17:00:40Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2013-10-05T16:53:01Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2239893
characteristics.characteristic: External Id
characteristics.text: CAUK
characteristics.characteristic: INSDC center name
characteristics.text: 2013-10-08T17:00:40Z
characteristics.characteristic: INSDC first public
characteristics.text: 2013-10-05T16:53:01Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS353620
characteristics.characteristic: SRA accession
characteristics.text: MYMC4100_5dABevo_C100r0_02
characteristics.characteristic: Submitter Id
characteristics.text: This population was derived by experimental evolution of Escherichia coli K-12 strain MYMC4100 (Schulenburg lab, Kiel, Germany). It was cultivated in the well of a 384-well microtiter plate in M9 minimal medium containing 0.15 µg/ml doxycycline and 9 µg/ml erythromycin. The population was propagated for five days with daily transfer into a new plate with fresh medium and fresh antibiotic(s) concentrations. The population visibly grew on day five of the experiment and was regrown for sequencing for a day without antibiotics.
characteristics.characteristic: description
characteristics.text: Escherichia coli K-12
characteristics.characteristic: organism
characteristics.text: Escherichia coli K-12
characteristics.characteristic: scientific_name
characteristics.text: MC4100
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002860
characteristics.characteristic: strain
characteristics.text: MYMC4100
characteristics.characteristic: sub strain
characteristics.text: Escherichia coli K-12 strain MYMC4100 evolved for five days with 0.15 µg/ml doxycycline and 9 µg/ml erythromycin in M9 minimal medium. Visible growth on day 5; 1 day regrowth for sequencing without antibiotics.
characteristics.characteristic: title