characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-05-01T17:00:44Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2014-03-24T15:38:37Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2422390
characteristics.characteristic: External Id
characteristics.text: Sars International Centre for Marine Molecular Biology
characteristics.characteristic: INSDC center name
characteristics.text: 2014-05-01T17:00:44Z
characteristics.characteristic: INSDC first public
characteristics.text: 2014-03-24T15:38:37Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS427297
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2430:non.reproductive_bottom_replica3
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: Protocols: Adult non-reproductive Sycon ciliatum specimens were collected on 25/10/12 from a fjord located near Bergen, Norway, and transported to laboratory in sea water collected at the place of their growth. They were gradually (1-3 hours) acclimatized to ambient temperature, avoiding temperatures exceeding 20 degrees. Field-collected water was gradually exchanged for sterile filtered natural sea water by replacing 1/4th to 1/3rd parts with the filtered water every 20-30 minutes 3-4 times. With aid of dissecting microscope, the samples were separated from the algae and cleaned from macroscopic contaminations by gently blowing water at them. After several water changes, the adults were cut transversely into slices 3-5mm thick. The top, bottom and two adjacent middle slices were selected for RNA isolation, pooling slices from eight individuals in each sample. None. Total RNA and DNA were isolated using the AllPrep DNA/RNA Mini Kit (Qiagen) according to manufacturerﾒs instructions. Adult tissue pieces were snap-frozen in liquid nitrogen and processed immediately or stored at -80 degrees until processing. In case of larvae, water was removed after centrifugation and replaced with the lysis buffer, and sample disrupted in 1.5 ml tubes in the buffer using a micropestle. In case of juveniles, which were attached to the bottom of the petri dishes, water was replaced with the lysis buffer and juveniles scraped of the bottom using cell culture scraper before transferring to 1.5 ml tubes and disruption. Isolated RNA and DNA were analysed on agarose gels and quantified by Nanodrop, some of the RNA samples were additionally analysed using Bioanalyzer. The libraries were prepared according to Illumina instructions for RNA sample preparation "TruSeq RNA V2".
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: Sycon ciliatum
characteristics.characteristic: organism
characteristics.text: Sycon ciliatum
characteristics.characteristic: organism
characteristics.text: bottom section
characteristics.characteristic: organism part
characteristics.text: Sycon ciliatum
characteristics.characteristic: scientific_name
characteristics.text: fresh
characteristics.characteristic: specimen with known storage state
characteristics.text: non.reproductive_bottom_replica3
characteristics.characteristic: title