characteristics.text: 2013-05-03T17:02:27Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2012-07-25T14:14:47Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA1886526
characteristics.characteristic: External Id
characteristics.text: Servicio de Secuenciacion masiva UC/IBBTEC, Santander Spain
characteristics.characteristic: INSDC center name
characteristics.text: 2013-05-03T17:02:27Z
characteristics.characteristic: INSDC first public
characteristics.text: 2012-07-25T14:14:47Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS158193
characteristics.characteristic: SRA accession
characteristics.text: 2308
characteristics.characteristic: StrainOrLine
characteristics.text: E-MTAB-1203:s5
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: Protocols: Two flasks containing 10 mls of fresh Brucella Broth were (each one) inoculated with 2 mls of an overnight culture of Brucella abortus 2308. Erythritol was added at 1 mg/ml to one of them and both cultures were then allowed to grow during 2.5 h until an OD600 = 0.6-0.7 RNA was purified by using the Qiagen RNeasy Protect bacteria mini kit (Quiagen, USA)and RNase free DNase I digestion. Pure RNA (5 ug) was depleted of rRNA with the Ambion MICROBExpress kit.
characteristics.characteristic: description
characteristics.text: Brucella abortus
characteristics.characteristic: organism
characteristics.text: Brucella abortus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCIT_C86216
characteristics.characteristic: organism
characteristics.text: Brucella abortus
characteristics.characteristic: scientific_name
characteristics.text: s5
characteristics.characteristic: title