characteristics.text: 2013-11-05T17:01:23Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2012-10-02T14:45:14Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SRA-Webin
characteristics.characteristic: ENA-SUBMISSION-TOOL
characteristics.text: SAMEA2189640
characteristics.characteristic: External Id
characteristics.text: UNIANDES_INGQUI
characteristics.characteristic: INSDC center name
characteristics.text: 2013-11-05T17:01:23Z
characteristics.characteristic: INSDC first public
characteristics.text: 2012-10-02T14:45:14Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS177844
characteristics.characteristic: SRA accession
characteristics.text: CHLRECCM1
characteristics.characteristic: Submitter Id
characteristics.text: UNIANDES
characteristics.characteristic: broker name
characteristics.text: C. reinhardtii (strain cc503 cw92 mt+) was cultured in 2-L flasks in photoautotrophic and temperature-controlled condition. Cells were cultured in H20P medium (modified TAP medium without acetate, supplemented with 20 mM HEPES as buffering agent, replacing Tris) under continuous light (~200 µE m-2 s-1) and constant supply of air with 5% CO2 (gas mixture was provided by a B-DCU Biostat fermenter, Sartorius, Goettingen, Germany, and controlled via a GC system, Agilent Technologies 3000 micro-GC, Waldbronn, Germany). After the cell culture had reached an optical density of 0.5 at 750 nm (approximately 3x106 cells · mL-1), 900 mL were sampled for e.g. transcript profiling, and the concentration of CO2 in the remaining culture was reduced to 0.04% in air. Samples of 20 mL of cell culture were then collected after 60 min and 120 min for transcript profiling, and 1 L of culture was sampled after 180 min for transcript profiling and FAIRE. Cells harvested for transcript analysis were collected by centrifugation (in 2-mL aliquots) at 3,000 g for 2 min and the cell pellets were then immediately frozen in liquid nitrogen. The samples were kept at -80°C until further use. Cells (900 mL) from the last time point (180 min) were directly subjected to formaldehyde crosslinking for FAIRE (see description below).
characteristics.characteristic: description
characteristics.text: mt+
characteristics.characteristic: mating type
characteristics.text: Chlamydomonas reinhardtii
characteristics.characteristic: organism
characteristics.text: Chlamydomonas reinhardtii
characteristics.characteristic: scientific_name
characteristics.text: cc503 cw92 mt+
characteristics.characteristic: strain
characteristics.text: Chlamydomonas reinhardtii cc503 cw92 mt+ under carbon deprivation
characteristics.characteristic: title