characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2016-04-20T17:01:17Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2014-07-31T23:20:40Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2677115
characteristics.characteristic: External Id
characteristics.text: Researcher
characteristics.characteristic: INSDC center name
characteristics.text: 2016-04-20T17:01:17Z
characteristics.characteristic: INSDC first public
characteristics.text: 2014-07-31T23:20:40Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS517007
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2779:64_l1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: Protocols: Two perennial ryegrass (Lolium perenne L.) genotypes, 'Falster' originated from Denmark, adapted to cold climates and 'Veyo' originated from Italy, adapted to warmer climates were propagated vegetatively in a glasshouse. Following propagation plants were grown in a glasshouse (photoperiod, 9 h : 15 h, light : dark) at ~20 degrees C for 12 weeks. Plants (at the pre-elongation stage) were then transferred to a growth chamber for 7 d (photoperiod, 9 h : 15 h, light : dark) with 450umol photons m-2 s-1 light intensity at 20 degrees C. Plants were cold acclimated in a controlled growth room for 17 d (photoperiod, 9 h : 15 h, light : dark) with 450umol photons m-2 s-1 light intensity and relative humidity of ~70% at 7 degrees C. Green tissue (top) samples were harvested in three biological replicates at the onset of the daily photoperiod at 0, 9, 13 and 17 d of 7 degrees C cold acclimation for RNA-Seq analysis. Samples were frozen in liquid nitrogen immediately after harvesting and stored at -80 degrees C until analysis. Leaf tissues were grounded separately in liquid nitrogen. Total RNA was extracted using RNeasy(r) Plant Mini Kit (Qiagen) and On-column DNAse I digestion (Qiagen) was performed to avoid the genomic DNA contamination according to the manufacturers' instructions. Total RNA quality was verified using an Agilent 2100 Bioanalyzer (RNA 6000 Nano Assay) and quantified by Quant-iTTM RiboGreen(r) RNA Reagent assay following the manufacturers' protocol. Total RNA samples were prepared for sequencing using the TruSeq RNA Sample Preparation Kit (Illumina).
characteristics.characteristic: description
characteristics.text: Falster
characteristics.characteristic: genotype
characteristics.text: Lolium perenne
characteristics.characteristic: organism
characteristics.text: Lolium perenne
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4522
characteristics.characteristic: organism
characteristics.text: leaf
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: organism part
characteristics.text: 1
characteristics.characteristic: replicate
characteristics.text: Lolium perenne
characteristics.characteristic: scientific_name
characteristics.text: 13 day
characteristics.characteristic: time for cold acclimation
characteristics.text: 64_l1
characteristics.characteristic: title