characteristics.text: 2013-06-27T17:00:22Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2013-01-29T17:30:20Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SRA-Webin
characteristics.characteristic: ENA-SUBMISSION-TOOL
characteristics.text: SAMEA2047075
characteristics.characteristic: External Id
characteristics.text: INRA-CMSE
characteristics.characteristic: INSDC center name
characteristics.text: 2013-06-27T17:00:22Z
characteristics.characteristic: INSDC first public
characteristics.text: 2013-01-29T17:30:20Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: 1.15 + or - 0.02 mg of DNA/g of soil organic carbon
characteristics.characteristic: Molecular Microbial Biomass
characteristics.text: ERS209843
characteristics.characteristic: SRA accession
characteristics.text: GnS_2012_LaosPL_ImprovedPasture_16S
characteristics.characteristic: Submitter Id
characteristics.text: 2010-29-06
characteristics.characteristic: collection date
characteristics.text: Improved pasture
characteristics.characteristic: current land use
characteristics.text: 0.10
characteristics.unit: m
characteristics.characteristic: depth
characteristics.text: DNA was extracted from a soil from a no-till improved pasture (impP) of Brachiaria ruziziensis conducted under intensive grazing. Bacterial diversity assessment was realized using 16S rDNA PCR amplification, and pyrosequencing.
characteristics.characteristic: description
characteristics.text: Tropical and subtropical grasslands, savannas, and shrubland biome
characteristics.characteristic: environment (biome)
characteristics.text: tropical soil
characteristics.characteristic: environment (feature)
characteristics.text: ferralsol
characteristics.characteristic: environment (material)
characteristics.text: soil
characteristics.characteristic: environmental package
characteristics.text: Laos, Poa village
characteristics.characteristic: geographic location (countryand/orsea,region)
characteristics.text: 1130
characteristics.unit: m
characteristics.characteristic: geographic location (elevation)
characteristics.text: 19.33
characteristics.unit: DD
characteristics.characteristic: geographic location (latitude)
characteristics.text: 102.59
characteristics.unit: DD
characteristics.characteristic: geographic location (longitude)
characteristics.text: metagenome
characteristics.characteristic: investigation type
characteristics.text: CGTATGCGAC
characteristics.characteristic: multiplex identifiers
characteristics.text: soil metagenome
characteristics.characteristic: organism
characteristics.text: initial denaturation:94degC_2.0min;35cycles of denaturation:94degC_0.5min;annealing:52degC_0.5min;elongation:72degC_1.0min;final elongation:72degC_7.0min
characteristics.characteristic: pcr conditions
characteristics.text: 338F:ACTCCTACGGGAGGCAGCAG; 803R:CTACCNGGGTATCTAAT
characteristics.characteristic: pcr primers
characteristics.text: 5.1
characteristics.characteristic: ph
characteristics.text: GnS_2012_LaosPL
characteristics.characteristic: project name
characteristics.text: 2
characteristics.unit: g
characteristics.characteristic: sample weight for DNA extraction
characteristics.text: soil metagenome
characteristics.characteristic: scientific_name
characteristics.text: Pyrosequencing
characteristics.characteristic: sequencing method
characteristics.text: 16s rRNA
characteristics.characteristic: target gene
characteristics.text: Bacterial diversity by 16S rRNA gene pyrosequencing of soil from a no-till improved pasture (impP) of Brachiaria ruziziensis conducted under intensive grazing
characteristics.characteristic: title
characteristics.text: 2.5
characteristics.unit: g/kg
characteristics.characteristic: total n
characteristics.text: 36.8
characteristics.unit: g/kg
characteristics.characteristic: total organic carbon