characteristics.text: 2013-09-07T17:00:44Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2013-04-23T13:24:45Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2066310
characteristics.characteristic: External Id
characteristics.text: Univ of Alabama at Birmingham
characteristics.characteristic: INSDC center name
characteristics.text: 2013-09-07T17:00:44Z
characteristics.characteristic: INSDC first public
characteristics.text: 2013-04-23T13:24:45Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS239310
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1581:NoMS-rep1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: Protocols: The cultures were of Verticillium dahliae, strain VdLs.17, that was producing (MS) or not producing microsclerotia (NoMS). The NoMS culture could be considered the control. Cultures were grown at 25 C on potato dextrose agar overlaid with a nitrocellulose membrane. All MS and NoMS cultures were ten days old. Total RNA was extracted from the pulverized fungal tissue using a Qiagen RNeasy Kit (Valencia, CA) and following the manufacturer's instructions, and included an on-column DNase I digestion. The sequncing library was constructed by using ScriptSeq_V2 RNA-Seq Library Preparation kit.
characteristics.characteristic: description
characteristics.text: Verticillium dahliae
characteristics.characteristic: organism
characteristics.text: Verticillium dahliae
characteristics.characteristic: organism
characteristics.text: not microsclerotia-producing
characteristics.characteristic: phenotype
characteristics.text: Verticillium dahliae
characteristics.characteristic: scientific_name
characteristics.text: fresh specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000971
characteristics.characteristic: specimen with known storage state
characteristics.text: strain VdLs.17
characteristics.characteristic: strain
characteristics.text: NoMS-rep1
characteristics.characteristic: title