characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2013-10-17T17:02:08Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2013-10-17T15:39:15Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2241658
characteristics.characteristic: External Id
characteristics.text: CEBITEC
characteristics.characteristic: INSDC center name
characteristics.text: 2013-10-17T17:02:08Z
characteristics.characteristic: INSDC first public
characteristics.text: 2013-10-17T15:39:15Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS361015
characteristics.characteristic: SRA accession
characteristics.text: Cg_Whole_Transcriptome
characteristics.characteristic: Submitter Id
characteristics.text: ATCC 13032
characteristics.characteristic: culture collection
characteristics.text: To obtain the RNA for the whole transcriptome, C. glutamicum cells were grown in shaking flasks in complex LB medium and defined CGXII medium and harvested in the exponential growth phase. In addition, C. glutamicum cells were grown in shaking flasks in defined CGXII medium and subjected to different stresses an OD600 of 10. High salt stress: addition of sodium chloride to a final concentration of 10 %. Heat shock stress: shift of the temperature from 30 °C to 50 °C. Cold shock stress: temperature shift from 30 °C to 4 °C. Alcohol stress: addition of ethanol to a final concentration of 10 %. Oxidative stress: addition of diamide to a final concentration of 2 mM; addition of hydrogen peroxide to a final concentration of 1 %. In each case, cells were harvested 15 min after exposure to stress conditions. Additionally, C. glutamicum cells were grown in a fermenter in defined CGXII medium to be able to apply high oxygen stress. Therefore, the dissolved oxygen level was shifted from 30 % to 90 %. After 2 hours of 90 % dissolved oxygen cells were harvested.
characteristics.characteristic: description
characteristics.text: Corynebacterium glutamicum ATCC 13032
characteristics.characteristic: organism
characteristics.text: Corynebacterium glutamicum ATCC 13032
characteristics.characteristic: scientific_name
characteristics.text: Cg_Transcriptome_Sequencing
characteristics.characteristic: title