characteristics.text: 2014-04-22T17:00:43Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2013-05-07T13:29:53Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2069335
characteristics.characteristic: External Id
characteristics.text: RIPCM
characteristics.characteristic: INSDC center name
characteristics.text: 2014-04-22T17:00:43Z
characteristics.characteristic: INSDC first public
characteristics.text: 2013-05-07T13:29:53Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS243298
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1637:P run2_b
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: protoplast
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000371
characteristics.characteristic: cell type
characteristics.text: Protocols: Moss gametophores were grown on modified agarized Knop medium (medium PPNO3) ?in 9cm Petri dishes. Protonema was grown on modified Knop medium and 500 mg/l of ammonium tartrate (medium PPNH4). Protoplasts were isolated from the protonema using a 0,5% solution of the enzyme Driselase (Sigma) in 0.48 M solution of mannitol.? Protonema, protoplast and gametophores were harvested and frozen. Total RNA was extracted by using the RNeasy Plant Mini Kit (Qiagen, USA) RNA libraries were prepared for sequencing using standard SOLiD? Total RNA-Seq Kit (PN 4452437 RevB) protocols.
characteristics.characteristic: description
characteristics.text: Physcomitrella patens
characteristics.characteristic: organism
characteristics.text: Physcomitrella patens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3218
characteristics.characteristic: organism
characteristics.text: Physcomitrella patens
characteristics.characteristic: scientific_name
characteristics.text: fresh specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000971
characteristics.characteristic: specimen with known storage state
characteristics.text: P run2_b
characteristics.characteristic: title