characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-07-04T11:15:22Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2014-07-04T08:51:17Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2627172
characteristics.characteristic: External Id
characteristics.text: EBI
characteristics.characteristic: INSDC center name
characteristics.text: 2014-07-04T11:15:22Z
characteristics.characteristic: INSDC first public
characteristics.text: 2014-07-04T08:51:17Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS497071
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1715_newsubmission:PL1S_1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: Protocols: T. brucei bloodstream form parasites (strain Lister 427, antigenic type MiTat 1.2, clone 221a) [Johnson and Cross, 1979] were cultured at 37 degrees C, 5% CO2, in HMI-11 medium. RNA interference of histone H1 was induced for 4 days in culture. Total RNA was extracted using TRIzol standard protocol. Ribosomal RNA was depleted with RiboMinus kit (Invitrogen). PolyA-containing RNA was purified using Dynabeads oligo-(dT) (Invitrogen) and cDNA was synthesized using random hexamers according to Complete RNA-seq Library System kit (NuGEN) instructions.
characteristics.characteristic: description
characteristics.text: bloodstream slender form
characteristics.characteristic: developmental stage
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: Trypanosoma brucei brucei
characteristics.characteristic: organism
characteristics.text: Trypanosoma brucei brucei
characteristics.characteristic: organism
characteristics.text: Trypanosoma brucei brucei
characteristics.characteristic: scientific_name
characteristics.text: fresh specimen
characteristics.characteristic: specimen with known storage state
characteristics.text: Lister 427
characteristics.characteristic: strain
characteristics.text: PL1S_1
characteristics.characteristic: title