characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-06-24T17:01:44Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2014-05-07T15:26:11Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2501492
characteristics.characteristic: External Id
characteristics.text: MRC Toxicology Unit, Leicester
characteristics.characteristic: INSDC center name
characteristics.text: 2014-06-24T17:01:44Z
characteristics.characteristic: INSDC first public
characteristics.text: 2014-05-07T15:26:11Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS451362
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2566:standard
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: sequencing library created using the standard Ion Torrent protocol Protocols: The RNA fragment pool 5' - GCAGUUGCCANNNNNNNNNN - 3' was synthesised (ThermoScientific Molecular Biology). Fragments contained both 5'phosphate and 3'hydroxyl moieties necessary for ligation. Ligation of 10 ng of RNA pool was performed with the Ion Total RNASeq kit v2 (Life Technologies), following the manufacturer's instructions.
characteristics.characteristic: description
characteristics.text: synthetic construct
characteristics.characteristic: organism
characteristics.text: synthetic construct
characteristics.characteristic: organism
characteristics.text: synthetic construct
characteristics.characteristic: scientific_name
characteristics.text: standard
characteristics.characteristic: title