characteristics.text: 2014-02-14T17:02:00Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2013-02-22T11:27:59Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2052677
characteristics.characteristic: External Id
characteristics.text: UCI-ECOEVI
characteristics.characteristic: INSDC center name
characteristics.text: 2014-02-14T17:02:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2013-02-22T11:27:59Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS216961
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1500:Hmel_Female_1_legs
characteristics.characteristic: Submitter Id
characteristics.text: 1 week
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UO_0000034
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: Protocols: Male and female Heliconius melpomene pupae were sampled from Costa Rica in 2012. We allowed the pupae to eclose as adults and then fed them for 1 week before freezing the tissue at -80 degrees centigrade for total RNA isolation. Total RNAs were extracted separately from antennae, proboscis together with labial palps, and all six legs of three individual males and three individual females of H. melpomene using Trizol (Life Technologies, Grand Island, NY). A NucleoSpin RNA II kit (Macherey-Nagel, Bethlehem, PA) was used to purify total RNAs. Each total RNA sample was purified through 1 NucleoSpin RNA II column. Purified total RNA samples were quantified using a Qubit 2.0 Fluorometer (Life Technologies, Grand Island, NY). The quality of the RNA samples was checked using an Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA). A TruSeq RNA sample prep kit (Illumina, San Diego, CA) was used to prepare 18 individual cDNA libraries. 0.3-4 ug of purified total RNAs were used to make cDNA libraries. After being normalized according to their concentrations, the enriched individual libraries were pooled and then run on a 2 0x0.0000000000001p-1022garose gel. cDNA products ranging from 280 to 340 bp with an average of 310 bp were cut out and purified using a Geneclean III kit (MP Biomedicals, Solon, OH) to facilitate post-sequencing assembly. After being re-purified using Agencourt AMPure XP magnetic beads (Beckman Coulter Genomics, Danvers, MA), the cDNA pool was quantified using the Qubit 2.0 Fluorometer, and QC checked using the Agilent Bioanalyzer 2100.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: wild_type
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000817
characteristics.characteristic: genotype
characteristics.text: Heliconius melpomene rosina
characteristics.characteristic: organism
characteristics.text: Heliconius melpomene rosina
characteristics.characteristic: organism
characteristics.text: leg
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000978
characteristics.characteristic: organism part
characteristics.text: Heliconius melpomene rosina
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: Hmel_Female_1_legs
characteristics.characteristic: title