characteristics.text: 2014-01-20T17:00:57Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2013-03-13T14:50:23Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2058685
characteristics.characteristic: External Id
characteristics.text: Centre for Research in Agrigenomics
characteristics.characteristic: INSDC center name
characteristics.text: 2014-01-20T17:00:57Z
characteristics.characteristic: INSDC first public
characteristics.text: 2013-03-13T14:50:23Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS223103
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1555:Maize conventional variety Tietar
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: Protocols: Plants were grow until maturity under controlled conditions in greenhouse / 5 Plants of each variety were crosspollinated / 1200 of 20DAP embryos were used per variety No differential treatment were done between transgenic and near-isogenic plants total RNA was extracted using TRIzol and cleaned-up by Rneasy column / mRNA was isolated using the Oligotex mRNA kit 3'UTR-anchored cDNA libraries were preparated according to Eveland AL, McCarty DR, Koch KE. (2008) Transcript profiling by 3'-untranslated region sequencing resolves expression of gene families. Plant Physiol. Jan;146(1):32-44.
characteristics.characteristic: description
characteristics.text: 20 DAP embryos
characteristics.characteristic: developmental stage
characteristics.text: wild type
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: Zea mays
characteristics.characteristic: organism
characteristics.text: Zea mays
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4577
characteristics.characteristic: organism
characteristics.text: Zea mays
characteristics.characteristic: scientific_name
characteristics.text: fresh specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000971
characteristics.characteristic: specimen with known storage state
characteristics.text: Maize conventional variety Tietar
characteristics.characteristic: title