characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-06-24T17:01:43Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2014-03-10T15:40:35Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2397575
characteristics.characteristic: External Id
characteristics.text: MRC National Institute for Medical Research
characteristics.characteristic: INSDC center name
characteristics.text: 2014-06-24T17:01:43Z
characteristics.characteristic: INSDC first public
characteristics.text: 2014-03-10T15:40:35Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS421629
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2390:WT_t0_7
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: Protocols: M. tuberculosis strains H37Rv (wild type) and M. tuberculosis Deltacrp were grown in Dubos broth containing 0.05% (v/v) Tween and supplemented with 0.5% (v/v) glycerol and 4% albumin. To monitor the response to nutrient starvation, M. tuberculosis was grown to mid-exponential phase (OD600 0.6), the cells pelleted and washed twice in PBS supplemented with 0.025% tyloxapol. Cells were then resuspended in 100 milliliters PBS plus tyloxapol and incubated without shaking at 37 degrees. For ChIPseq, M. tuberculosis cells were grown in roller bottles to mid-exponential phase and formaldehyde was added to a final concentration of 1 percent. After 10 minutes of incubation, glycine was added and cross-linked cells were harvested. RNA was extracted from exponentially growing wild type and Deltacrp M. tuberculosis H37Rv as previously described (E-MTAB-1616). Samples were sequenced on an illumina Genome Analyzer IIx as single end reads
characteristics.characteristic: description
characteristics.text: wild type
characteristics.characteristic: genotype
characteristics.text: exponential
characteristics.characteristic: growth condition
characteristics.text: Mycobacterium tuberculosis
characteristics.characteristic: organism
characteristics.text: Mycobacterium tuberculosis
characteristics.characteristic: organism
characteristics.text: gene expression exponential growth
characteristics.characteristic: phenotype
characteristics.text: Mycobacterium tuberculosis
characteristics.characteristic: scientific_name
characteristics.text: fresh specimen
characteristics.characteristic: specimen with known storage state
characteristics.text: H37Rv
characteristics.characteristic: strain
characteristics.text: WT_t0_7
characteristics.characteristic: title