characteristics.text: 2016-04-30T17:10:00Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2013-04-08T16:22:22Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA1964217
characteristics.characteristic: External Id
characteristics.text: EMBL-EBI
characteristics.characteristic: INSDC center name
characteristics.text: 2016-04-30T17:10:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2013-04-08T16:22:22Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS233514
characteristics.characteristic: SRA accession
characteristics.text: E-SYBR-7:t2KO2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: Protocols: These are laboratory strains genetically engineered from strain SN148 which were not submitted to infection assays. Strains CIP,t1,t2 have 0, 1 and 2 copies of a Leu-tRNA(CAG) gene inserted at RPS10 locus, respectively. Strain t2ko1 is derived from strain t2 by deletion of one copy of the Ser-tRNA(CAG) gene. Strain t2ko2 is derived from t2ko1 by deletion of the second copy of the Ser-tRNA(CAG) gene. Samples were taken from the stock and plated onto YPD agar media containing fluconazole. One colony was selected for growth in liquid YPD until exponential phase. DNA extraction was carried out using the Genomic-tip 100/G kit (Qiagen) according to the manufacturer's protocol. Quantification and quality assessment were performed using the Picogreen fluorescence based quantification assay. Libraries were constructed using Illumina TruSeq DNA Sample Prep standard protocol. Briefly, 5 ug of high molecular weight genomic DNA (gDNA) was fragmented by Covaris sonication device. Following sonication, DNA fragments were end-repaired and A-tailed. Adapters were then ligated via a 3' thymine overhang. Finally, ligated fragments were amplified by PCR (10 cycles). insert sizes were about 400-500 bp as evaluated in an Agilent DNA 1000 Analyzer Chip.
characteristics.characteristic: description
characteristics.text: Candida albicans
characteristics.characteristic: organism
characteristics.text: Candida albicans
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_5476
characteristics.characteristic: organism
characteristics.text: 97 percent leucin translation at CTG codon sites
characteristics.characteristic: phenotype
characteristics.text: Candida albicans
characteristics.characteristic: scientific_name
characteristics.text: fresh sample
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000971
characteristics.characteristic: specimen of known storage state
characteristics.text: t2KO2
characteristics.characteristic: strain
characteristics.text: t2KO2
characteristics.characteristic: title