characteristics.text: ERC000013
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2013-08-07T17:00:27Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2016-10-21T09:38:51Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SRA-Webin
characteristics.characteristic: ENA-SUBMISSION-TOOL
characteristics.text: SAMEA2169215
characteristics.characteristic: External Id
characteristics.text: MHH
characteristics.characteristic: INSDC center name
characteristics.text: 2013-08-07T17:00:27Z
characteristics.characteristic: INSDC first public
characteristics.text: 2016-10-21T09:38:51Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS311688
characteristics.characteristic: SRA accession
characteristics.text: MIT colon mouse 4
characteristics.characteristic: Submitter Id
characteristics.text: 5'-GCCTTGCCAGCCCGCTCAG-multiplex_idendifier-CA; 5'-GCCTTGCCAGCCCGCTCAGTC-3'
characteristics.characteristic: adapters
characteristics.text: 172
characteristics.unit: day
characteristics.characteristic: age
characteristics.text: colon
characteristics.characteristic: body habitat
characteristics.text: colon
characteristics.characteristic: body site
characteristics.text: 10.12.2009
characteristics.characteristic: collection date
characteristics.text: Animal facility
characteristics.characteristic: environment (biome)
characteristics.text: specific pathogen-free
characteristics.characteristic: environment (feature)
characteristics.text: air
characteristics.characteristic: environment (material)
characteristics.text: Il10-/-
characteristics.characteristic: genotype
characteristics.text: USA
characteristics.characteristic: geographic location (countryand/orsea,region)
characteristics.text: 42.3591326
characteristics.unit: DD
characteristics.characteristic: geographic location (latitude)
characteristics.text: -71.093201
characteristics.unit: DD
characteristics.characteristic: geographic location (longitude)
characteristics.text: mouse
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10088
characteristics.characteristic: host common name
characteristics.text: MIT mouse4
characteristics.characteristic: host subject id
characteristics.text: 10090
characteristics.characteristic: host taxid
characteristics.text: host-associated
characteristics.characteristic: host-associated environmental package
characteristics.text: C57BL/6J
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000606
characteristics.characteristic: infra_specific_rank
characteristics.text: mimarks-survey
characteristics.characteristic: investigation type
characteristics.text: MIT
characteristics.characteristic: mouse batch
characteristics.text: AGCACTGTAG
characteristics.characteristic: multiplex identifiers
characteristics.text: mouse gut metagenome
characteristics.characteristic: organism
characteristics.text: 50 µl reaction containing 30-50 ng DNA, 0.3 µM of each primer, 2.5 mM Mg2+, 0.2 mM of each dNTP, 2.5 U HotMaster Taq Polymerase (5PRIME). Initial denaturation: 95degC_ 2 min; denaturation: 95degC_20 s; annealing: 52degC_20s; elongation: 65degC_1min; 30 cycles.
characteristics.characteristic: pcr conditions
characteristics.text: 8F (5'-AGAGTTTGATCCTGGCTCAG-3'), 338R (5'-TGCTGCCTCCCGTAGGAGT-3'); sequencing primer: 338R
characteristics.characteristic: pcr primers
characteristics.text: Molecular Determinants of Helicobacter hepaticus pathogenesis
characteristics.characteristic: project name
characteristics.text: mouse gut metagenome
characteristics.characteristic: scientific_name
characteristics.text: pyrosequencing
characteristics.characteristic: sequencing method
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: 16S rDNA
characteristics.characteristic: target gene
characteristics.text: V1 - V2
characteristics.characteristic: target subfragment
characteristics.text: Intestinal microbiota of interleukin-10 deficient C57BL/6J mice.
characteristics.characteristic: title