characteristics.text: ERC000022
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-12-31T17:01:12Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2016-10-21T09:33:13Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2730092
characteristics.characteristic: External Id
characteristics.text: INRA-CMSE
characteristics.characteristic: INSDC center name
characteristics.text: 2015-12-31T17:01:12Z
characteristics.characteristic: INSDC first public
characteristics.text: 2016-10-21T09:33:13Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS450380
characteristics.characteristic: SRA accession
characteristics.text: LUS_18S_P3-C-T3
characteristics.characteristic: Submitter Id
characteristics.text: 200
characteristics.unit: g
characteristics.characteristic: amount or size of sample collected
characteristics.text: 2011-10-05
characteristics.characteristic: collection date
characteristics.text: 0.1
characteristics.unit: m
characteristics.characteristic: depth
characteristics.text: temperate grasslands
characteristics.characteristic: environment (biome)
characteristics.text: field soil
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/ENVO_00005755
characteristics.characteristic: environment (feature)
characteristics.text: Cambisol
characteristics.characteristic: environment (material)
characteristics.text: France
characteristics.characteristic: geographic location (countryand/orsea,region)
characteristics.text: 85
characteristics.unit: m
characteristics.characteristic: geographic location (elevation)
characteristics.text: 46.413789
characteristics.unit: DD
characteristics.characteristic: geographic location (latitude)
characteristics.text: 0.122089
characteristics.unit: DD
characteristics.characteristic: geographic location (longitude)
characteristics.text: permanent cultivated grassland
characteristics.characteristic: history/crop rotation
characteristics.text: grassland
characteristics.characteristic: history/previous land use
characteristics.text: mimarks-survey
characteristics.characteristic: investigation type
characteristics.text: CGCGACATCT
characteristics.characteristic: multiplex identifiers
characteristics.text: soil metagenome
characteristics.characteristic: organism
characteristics.text: 5ng of DNA were used for a 25 µl PCR conducted under the following conditions: initial denaturation : 94degC_3min; 35 cycles of: denaturation : 94degC_1min; annealing : 52degC_1min ; elongation: 72degC_1min, final elongation : 72degC_5min. A second PCR of 9 cycles was then conducted under similar PCR conditions with purified PCR products and ten base pair multiplex identifiers added to the primers at 5’ position to specifically identify each sample and avoid PCR biases.
characteristics.characteristic: pcr conditions
characteristics.text: FR1 (5’-ANCCATTCAATCGGTANT-3’) FF390 (5’-CGATAACGAACGAGACCT-3’)
characteristics.characteristic: pcr primers
characteristics.text: 6.05
characteristics.characteristic: ph
characteristics.text: LAND-USE_LUSIGNAN_2014
characteristics.characteristic: project name
characteristics.text: 1
characteristics.unit: g
characteristics.characteristic: sample weight for DNA extraction
characteristics.text: soil metagenome
characteristics.characteristic: scientific_name
characteristics.text: 454-pyrosequencing
characteristics.characteristic: sequencing method
characteristics.text: soil
characteristics.characteristic: soil environmental package
characteristics.text: Cambisol
characteristics.characteristic: soil type
characteristics.text: lyophilized + frozen
characteristics.characteristic: storage conditions (fresh/frozen/other)
characteristics.text: 18S rRNA gene
characteristics.characteristic: target gene
characteristics.text: 18/69/13
characteristics.unit: % sand/silt/clay
characteristics.characteristic: texture
characteristics.text: Land_use_LUSIGNAN_soil_bacteria_fungi_diversity_study
characteristics.characteristic: title
characteristics.text: 0.16
characteristics.unit: g/g
characteristics.characteristic: water content of soil