characteristics.text: ERC000013
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-06-17T17:01:14Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2016-10-21T09:38:55Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2341064
characteristics.characteristic: External Id
characteristics.text: MPIEVB
characteristics.characteristic: INSDC center name
characteristics.text: 2015-06-17T17:01:14Z
characteristics.characteristic: INSDC first public
characteristics.text: 2016-10-21T09:38:55Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS398735
characteristics.characteristic: SRA accession
characteristics.text: GG06_7d_0
characteristics.characteristic: Submitter Id
characteristics.text: 2012-08-28
characteristics.characteristic: collection date
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Glycans on mucosal surfaces play an important role in host-microbe interactions. Expression of the blood group-related glycosyltransferase ?-1,4-N-acetylgalactosaminyltransferase 2 (B4galnt2) is variable in wild mouse populations and influences the composition of the intestinal microbiota. In this study we show that the presence of B4galnt2 glycans in the intestinal epithelium increases susceptibility to Salmonella Typhimurium infection, leading to increased levels of pro-inflammatory cytokines and higher numbers of infiltrating immune cells. Further, changes in the intestinal microbiota correlated significantly with the development of inflammation and the associated B4galnt2 genotype. Specifically, the higher phylogenetic diversity of the intestinal microbiota present in B4galnt2-deficient mice before infection was predictive for a reduced inflammatory response to Salmonella Typhimurium. These data support the hypothesis that variation in B4galnt2 expression and associated microbial patterns alters host susceptibility to infectious diseases of the gastrointestinal tract and may explain the patterns of long term balancing selection observed in wild mouse populations.
characteristics.characteristic: description
characteristics.text: Streptomycin treated
characteristics.characteristic: disease status
characteristics.text: organism-associated habitat
characteristics.characteristic: environment (biome)
characteristics.text: organism-associated habitat
characteristics.characteristic: environment (feature)
characteristics.text: feces
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCIT_C13234
characteristics.characteristic: environment (material)
characteristics.text: genetic variation, bacterial disease
characteristics.characteristic: experimental factor
characteristics.text: endothel= -; epithelia= +/-
characteristics.characteristic: genotype
characteristics.text: Germany
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: Germany
characteristics.characteristic: geographic location (countryand/orsea,region)
characteristics.text: 53.821150
characteristics.unit: DD
characteristics.characteristic: geographic location (latitude)
characteristics.text: 10.193635
characteristics.unit: DD
characteristics.characteristic: geographic location (longitude)
characteristics.text: GG06_7
characteristics.characteristic: host subject id
characteristics.text: 10090
characteristics.characteristic: host taxid
characteristics.text: host-associated
characteristics.characteristic: host-associated environmental package
characteristics.text: metagenome
characteristics.characteristic: investigation type
characteristics.text: TACTCTCGTGCA
characteristics.characteristic: multiplex identifiers
characteristics.text: MOBIO PowerSoil DNA Isolation Kit
characteristics.characteristic: nucleic acid extraction
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: FWD:TGCTGCCTCCCGTAGGAGT; REV: AGAGTTTGATCCTGGCTCAG
characteristics.characteristic: pcr primers
characteristics.text: B4Galnt2
characteristics.characteristic: project name
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: pyrosequencing
characteristics.characteristic: sequencing method
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: 16S rRNA
characteristics.characteristic: target gene
characteristics.text: V1,V2
characteristics.characteristic: target subfragment
characteristics.text: Salmonella infection in B4Galnt2 knock out mice and transgenic B4Galnt2 mice
characteristics.characteristic: title