characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2016-12-02T17:05:14Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2016-02-04T17:04:40Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA3724774
characteristics.characteristic: External Id
characteristics.text: Stazione Zoologica \Anton Dohrn\
characteristics.characteristic: INSDC center name
characteristics.text: 2016-12-02T17:05:14Z
characteristics.characteristic: INSDC first public
characteristics.text: 2016-02-04T17:04:40Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1031923
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-4232:T07_1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: Protocols: Prior to start the CO2 treatment plants were left to recover from transportation, and monitored for five days after transplantation (i.e. acclimated). At the end of the acclimation period (t0), C. nodosa was either kept under present day (400 ppm), or pCO2 elevated to 1,200 ppm, close to the IPCC business as usual scenario for 2100. In total, five tanks were set at 400 ppm whereas the other five were maintained at pCO2 of 1,200 ppm. Plant material for RNA extraction and subsequent transcriptome sequencing was sampled at t0, and after 12 days of exposure to current or elevated pCO2 (t1) from three of the five experimental tanks treatment. All samples were taken between 11 and 13 h to avoid circadian modulation of gene expression, rapidly cleaned of epiphytes and entirely submerged in RNAlater tissue collection (Ambion, life technologies), to inhibit RNA degradation. After let the solution penetrate throughout the tissue for one night at 4C, leaf samples were definitely stored at -20C until RNA extraction. Total RNA from youngest fully developed leaves of Cymodocea nodosa ramets, was extracted with AurumTM Total RNA Mini Kit (BIO-RAD) following manufacturers protocol. Qualified RNA samples were sent to Personal Genomics s.r.l. (Verona, Italy) for libraries preparation and sequencing. Nine cDNA libraries were constructed according to the Illumina TruSeq Stranded mRNA library protocol outlined in TruSeq Stranded mRNA Sample Preparation Guide (Part # 15031047; Rev. E; October 2013), from 2.5 g total RNA. The libraries were subsequently size selected using the Pippin Prep automated gel electrophoresis system (Sage Science) for 350 to 550 bp.
characteristics.characteristic: description
characteristics.text: CO2 400ppm
characteristics.characteristic: growth condition
characteristics.text: T07_1
characteristics.characteristic: isolate
characteristics.text: Cymodocea nodosa
characteristics.characteristic: organism
characteristics.text: Cymodocea nodosa
characteristics.characteristic: organism
characteristics.text: leaf
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: organism part
characteristics.text: Cymodocea nodosa
characteristics.characteristic: scientific_name
characteristics.text: Control (CO2 400ppm)
characteristics.characteristic: stimulus
characteristics.text: T07_1
characteristics.characteristic: title