characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2016-03-03T17:02:09Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2016-02-03T11:12:12Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA3864480
characteristics.characteristic: External Id
characteristics.text: Wolfson Wohl Cancer Research Centre, Institute of Cancer Sciences, University of Glasgow
characteristics.characteristic: INSDC center name
characteristics.text: 2016-03-03T17:02:09Z
characteristics.characteristic: INSDC first public
characteristics.text: 2016-02-03T11:12:12Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1051614
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-4415:KPflC-3
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: mouse cell line
characteristics.characteristic: cell line
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Unknown RNA-Seq libraries were generated using the Illumina TruSeq Stranded Total RNA LT sample preparation kit (with Ribo-Zero Gold) (Illumina, Part no. RS-122-2301 and RS-1222302), according to the standard manufacturers protocol (Part no. 15031048 Rev. D April 2013), except they were made in an automated high-throughput fashion using Perkin Elmers Sciclone G3 NGS Workstation (Product no. SG3-31020-0300). The ribosomal depletion step was performed on 1 g of total RNA using Ribo-Zero Gold prior to a heat fragmentation step aimed at producing libraries with an insert size between 120-200 bp. cDNA was then synthesized from the enriched and fragmented RNA using SuperScript II Reverse Transcriptase (Invitrogen, Catalog no. 18064) and random primers. The resulting cDNA was converted into double stranded DNA in the presence of dUTP to prevent subsequent amplification of the second strand and thus maintain the strandedness of the library. Following 3 adenylation and adaptor ligation, libraries were subjected to 15 cycles of PCR to produce RNA-Seq libraries ready for sequencing. Prior to sequencing, RNA-Seq libraries were qualified via the Perkin Elmer LabChip GX with the DNA High Sensitivity LabChip kit (Perkin Elmer, Part no. CLS760672). Quantification of libraries for clustering was performed using the KAPA Library Quantification Kit - Illumina/Universal (KAPA Biosystems, Part no. KK4824) in combination with the Life Technologies Viia 7 real time PCR instrument.
characteristics.characteristic: description
characteristics.text: 3
characteristics.characteristic: individual
characteristics.text: KRAS Trp53fl/+
characteristics.characteristic: kras status
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: KPflC-3
characteristics.characteristic: title