characteristics.text: ERC000013
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2016-06-29T17:01:56Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2016-10-21T09:39:27Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA3461820
characteristics.characteristic: External Id
characteristics.text: ALFRED WEGENER INSTITUTE FOR POLAR AND MARINE RESE
characteristics.characteristic: INSDC center name
characteristics.text: 2016-06-29T17:01:56Z
characteristics.characteristic: INSDC first public
characteristics.text: 2016-10-21T09:39:27Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS756718
characteristics.characteristic: SRA accession
characteristics.text: Cg_1_Hem_Sy_SyA_20120704
characteristics.characteristic: Submitter Id
characteristics.text: 0.002
characteristics.unit: L
characteristics.characteristic: amount or size of sample collected
characteristics.text: Mix of ampiciline, gentamicine, tetracycline and kanamycine, each 100 µg/l seawater each
characteristics.characteristic: chemical administration
characteristics.text: 2012-07-04
characteristics.characteristic: collection date
characteristics.text: Pacific oyster
characteristics.characteristic: common name
characteristics.text: Pacific oyster (Crassostrea gigas) hemolymph microbiota; collected on Sylt, 2012-07-04; sample taken after field deployment; treated with mix of ampiciline, gentamicine, tetracycline and kanamycine (100 µg/l seawater each) between 2012-05-29 and 2012-06-01; oyster from Sylt, not translocated
characteristics.characteristic: description
characteristics.text: aquatic, marine, benthic, coastal
characteristics.characteristic: environment (biome)
characteristics.text: organic
characteristics.characteristic: environment (feature)
characteristics.text: organic material, bodily fluid
characteristics.characteristic: environment (material)
characteristics.text: North Sea
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: 55.02853237
characteristics.unit: DD
characteristics.characteristic: geographic location (latitude)
characteristics.text: 8.434868698
characteristics.unit: DD
characteristics.characteristic: geographic location (longitude)
characteristics.text: Schleswig Holstein, Sylt, List, Odewatt
characteristics.characteristic: geographic location (region and locality)
characteristics.text: hemolymph
characteristics.characteristic: host body habitat
characteristics.text: adductor muscle
characteristics.characteristic: host body site
characteristics.text: Pacific oyster
characteristics.characteristic: host common name
characteristics.text: adult
characteristics.characteristic: host life stage
characteristics.text: Sy_1
characteristics.characteristic: host subject id
characteristics.text: 29159
characteristics.characteristic: host taxid
characteristics.text: host-associated
characteristics.characteristic: host-associated environmental package
characteristics.text: mimarks-survey
characteristics.characteristic: investigation type
characteristics.text: f: ACCAGTTG; r: ACAGCTCA
characteristics.characteristic: multiplex identifiers
characteristics.text: Crassostrea gigas
characteristics.characteristic: organism
characteristics.text: initial denaturation:98degC_30s; denaturation:98degC_9s;annealing:55degC_1min; elongation:72degC_90s; final elongation:72degC_10min; 30 cycles
characteristics.characteristic: pcr conditions
characteristics.text: 27f-338r
characteristics.characteristic: pcr primers
characteristics.text: 27F: TCAGAGTTTGATCMTGGCTCAG + 338r: CATGCTGCCTCCCGTAGGAGT
characteristics.characteristic: pcr primers
characteristics.text: treated with mix of ampiciline, gentamicine, tetracycline and kanamycine (100 µg/l seawater each) between 2012-05-29 and 2012-06-01
characteristics.characteristic: perturbation
characteristics.text: PRJEB9624
characteristics.characteristic: project name
characteristics.text: syringe
characteristics.characteristic: sample collection device or method
characteristics.text: -80
characteristics.unit: ºC
characteristics.characteristic: sample storage temperature
characteristics.text: 0.2
characteristics.unit: mL
characteristics.characteristic: sample volume or weight for DNA extraction
characteristics.text: Crassostrea gigas
characteristics.characteristic: scientific_name
characteristics.text: Sequencing by synthesis, Illumina paired-end
characteristics.characteristic: sequencing method
characteristics.text: 16s rRNA
characteristics.characteristic: target gene
characteristics.text: V1-V2
characteristics.characteristic: target subfragment
characteristics.text: 17.725
characteristics.unit: ºC
characteristics.characteristic: temperature
characteristics.text: Pacific oyster hemolymph microbiota from Sylt
characteristics.characteristic: title