characteristics.text: ERC000022
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-06-29T17:01:59Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2016-10-21T09:33:37Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA3367619
characteristics.characteristic: External Id
characteristics.text: ETH
characteristics.characteristic: INSDC center name
characteristics.text: 2015-06-29T17:01:59Z
characteristics.characteristic: INSDC first public
characteristics.text: 2016-10-21T09:33:37Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS715271
characteristics.characteristic: SRA accession
characteristics.text: phoD_NPK_DNA
characteristics.characteristic: Submitter Id
characteristics.text: 2012-09-23
characteristics.characteristic: collection date
characteristics.text: grassland
characteristics.characteristic: current land use
characteristics.text: 0.05
characteristics.unit: m
characteristics.characteristic: depth
characteristics.text: We assessed the effects of soil pH and P fertilization on the phoD gene and transcript in a long-term grassland trial characterized by a natural pH gradient and identified the key bacteria that express and carry the phoD alkaline phosphatase gene using 454-sequencing.
characteristics.characteristic: description
characteristics.text: Grassland soil
characteristics.characteristic: environment (biome)
characteristics.text: Rich grassland
characteristics.characteristic: environment (feature)
characteristics.text: 2g soil
characteristics.characteristic: environment (material)
characteristics.text: Switzerland
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: 457
characteristics.unit: m
characteristics.characteristic: geographic location (elevation)
characteristics.text: 47.4389
characteristics.unit: DD
characteristics.characteristic: geographic location (latitude)
characteristics.text: 8.6819
characteristics.unit: DD
characteristics.characteristic: geographic location (longitude)
characteristics.text: metagenome
characteristics.characteristic: investigation type
characteristics.text: RNA Powersoil® Total RNA isolation kit, MoBio
characteristics.characteristic: nucleic acid extraction
characteristics.text: soil metagenome
characteristics.characteristic: organism
characteristics.text: phoD-F733 5'-TGGGAYGATCAYGARGT-3' / phoD-R1083 5'-CTGSGCSAKSACRTTCCA-3'
characteristics.characteristic: pcr primers
characteristics.text: 5.3
characteristics.characteristic: ph
characteristics.text: phoD alkaline phosphatase in a swiss lowland grassland
characteristics.characteristic: project name
characteristics.text: soil metagenome
characteristics.characteristic: scientific_name
characteristics.text: 454-sequencing
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005016
characteristics.characteristic: sequencing method
characteristics.text: soil
characteristics.characteristic: soil environmental package
characteristics.text: Cambisol
characteristics.characteristic: soil taxonomic/fao classification
characteristics.text: Cambisol
characteristics.characteristic: soil type
characteristics.text: phoD alkaline phosphatase
characteristics.characteristic: target gene
characteristics.text: phoD alkaline phosphatase in a grassland
characteristics.characteristic: title