characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2017-02-01T17:01:36Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2016-02-17T13:58:31Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA3876297
characteristics.characteristic: External Id
characteristics.text: Institute for Molecular Bioscience, University of Queenland, Brisbane, QLD 4072, Australia
characteristics.characteristic: INSDC center name
characteristics.text: 2017-02-01T17:01:36Z
characteristics.characteristic: INSDC first public
characteristics.text: 2016-02-17T13:58:31Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1063431
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-4481:Sox18_cMyc_INPUT
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: HUVEC
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002451
characteristics.characteristic: cell line
characteristics.text: human umbilical vein endothelial cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0002618
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: HUVECs were obtained from LONZA and grown according to suppliers recommendations. Cells were cultured in EBM2 media, supplemented with EGM2. Transfection was performed using Roche X-tremegene DNA transfection reagent in low (0.4%) FBS Following IP, DNA amplification was performed using TruSeq ChIPseq kit (Illumina, IP-202-1012). The PCR product was purified using AMPure beads. The library was quantified using the KAPA library quantification kit for Illumina sequencing platforms (KK4824)
characteristics.characteristic: description
characteristics.text: transfected with Sox18-cMyc
characteristics.characteristic: genotype
characteristics.text: EGM2 supplemented media + vascular endothelial growth factor A
characteristics.characteristic: growth condition
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Sox18_cMyc_INPUT
characteristics.characteristic: title