characteristics.text: ERC000013
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2016-01-14T17:03:09Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2016-10-21T09:39:14Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA3255625
characteristics.characteristic: External Id
characteristics.text: ALFRED WEGENER INSTITUTE FOR POLAR AND MARINE RESE
characteristics.characteristic: INSDC center name
characteristics.text: 2016-01-14T17:03:09Z
characteristics.characteristic: INSDC first public
characteristics.text: 2016-10-21T09:39:14Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS660604
characteristics.characteristic: SRA accession
characteristics.text: Cg167_Hem_SySyC_Jn4
characteristics.characteristic: Submitter Id
characteristics.text: 2012-06-04
characteristics.characteristic: collection date
characteristics.text: Pacific oyster
characteristics.characteristic: common name
characteristics.text: Pacific oyster (Crassostrea gigas) hemolymph microbiota; collected 04-Jun-2012; treated with None , not translocated (Sylt, D); samples taken 3 days after deployment
characteristics.characteristic: description
characteristics.text: aquatic, marine, benthic, coastal
characteristics.characteristic: environment (biome)
characteristics.text: organic
characteristics.characteristic: environment (feature)
characteristics.text: organic material, bodily fluid
characteristics.characteristic: environment (material)
characteristics.text: North Sea
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: 55.028
characteristics.unit: DD
characteristics.characteristic: geographic location (latitude)
characteristics.text: 8.434
characteristics.unit: DD
characteristics.characteristic: geographic location (longitude)
characteristics.text: Germany, Schleswig Holstein, Sylt, List, Wadden Sea, Odewatt
characteristics.characteristic: geographic location (region and locality)
characteristics.text: hemolymph
characteristics.characteristic: host body habitat
characteristics.text: adductor muscle
characteristics.characteristic: host body site
characteristics.text: Pacific oyster
characteristics.characteristic: host common name
characteristics.text: adult
characteristics.characteristic: host life stage
characteristics.text: 167
characteristics.characteristic: host subject id
characteristics.text: 29159
characteristics.characteristic: host taxid
characteristics.text: host-associated
characteristics.characteristic: host-associated environmental package
characteristics.text: mimarks-survey
characteristics.characteristic: investigation type
characteristics.text: f: ACCAGTTG; r: CACAAGTC
characteristics.characteristic: multiplex identifiers
characteristics.text: Crassostrea gigas
characteristics.characteristic: organism
characteristics.text: initial denaturation:98degC_30s; denaturation:98degC_9s;annealing:55degC_1min; elongation:72degC_90s; final elongation:72degC_10min; 30 cycles
characteristics.characteristic: pcr conditions
characteristics.text: 27f-338r
characteristics.characteristic: pcr primers
characteristics.text: treated with None , not translocated (Sylt, D)
characteristics.characteristic: perturbation
characteristics.text: PRJEB8492
characteristics.characteristic: project name
characteristics.text: syringe
characteristics.characteristic: sample collection device or method
characteristics.text: 0.2
characteristics.unit: mL
characteristics.characteristic: sample volume or weight for DNA extraction
characteristics.text: Crassostrea gigas
characteristics.characteristic: scientific_name
characteristics.text: Sequencing by synthesis, Illumina paired-end
characteristics.characteristic: sequencing method
characteristics.text: 16s rRNA
characteristics.characteristic: target gene
characteristics.text: V1-V2
characteristics.characteristic: target subfragment
characteristics.text: 14
characteristics.unit: ºC
characteristics.characteristic: temperature
characteristics.text: Pacific oyster hemolymph microbiota, treated with None , not translocated
characteristics.characteristic: title