characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2017-10-27T17:02:47Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2016-03-15T17:50:10Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA3900910
characteristics.characteristic: External Id
characteristics.text: UCSF
characteristics.characteristic: INSDC center name
characteristics.text: 2017-10-27T17:02:47Z
characteristics.characteristic: INSDC first public
characteristics.text: 2016-03-15T17:50:10Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1088044
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-4528:Sample 1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: The mice were kept under 6C for 5 days. Skeletal muscle tissue was collected from “beta-less mice”, which have been described in Bachmann et al. Science, 2002, “βAR Signaling Required for Diet-Induced Thermogenesis and Obesity Resistance”. RNA from soleus skeletal muscle was extracted by using RNeasy Mini Kit (QIAGEN). The cDNA libraries were amplified by single primer isothermal amplification using an Ultralow DR library kit (NuGEN) according to the manufacturer’s instructions. The qualities of the libraries were determined by Bioanalyzer (Agilent Technologies).
characteristics.characteristic: description
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: skeletal muscle organ
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0014892
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: control
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001461
characteristics.characteristic: stimulus
characteristics.text: Sample 1
characteristics.characteristic: title