characteristics.text: ERC000021
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-08-31T17:01:00Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2016-10-21T09:35:34Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA3111968
characteristics.characteristic: External Id
characteristics.text: UNIVERSITY OF HELSINKI
characteristics.characteristic: INSDC center name
characteristics.text: 2015-08-31T17:01:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2016-10-21T09:35:34Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS579189
characteristics.characteristic: SRA accession
characteristics.text: S09SP
characteristics.characteristic: Submitter Id
characteristics.text: 2009-04
characteristics.characteristic: collection date
characteristics.text: 33
characteristics.unit: m
characteristics.characteristic: depth
characteristics.text: bacterial 16S rDNA oxic sediment layer (0-0.5cm)
characteristics.characteristic: description
characteristics.text: coastal area
characteristics.characteristic: environment (biome)
characteristics.text: outer archipelago
characteristics.characteristic: environment (feature)
characteristics.text: sediment
characteristics.characteristic: environment (material)
characteristics.text: Baltic Sea
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: 0
characteristics.unit: m
characteristics.characteristic: geographic location (elevation)
characteristics.text: 59.855167
characteristics.unit: DD
characteristics.characteristic: geographic location (latitude)
characteristics.text: 23.263667
characteristics.unit: DD
characteristics.characteristic: geographic location (longitude)
characteristics.text: mimarks-survey
characteristics.characteristic: investigation type
characteristics.text: CAGCTC
characteristics.characteristic: multiplex identifiers
characteristics.text: Phusion Polymerase
characteristics.characteristic: nucleic acid amplification
characteristics.text: doi: 10.1128/AEM.66.12.5488-5491.2000
characteristics.characteristic: nucleic acid extraction
characteristics.text: unidentified
characteristics.characteristic: organism
characteristics.text: initial denaturation:98degC_30s;cycles:17;denaturation:10s_98degC,annealing:65degC_30s;extension:72degC_10S;final extension:72degC_5min
characteristics.characteristic: pcr conditions
characteristics.text: 27F (5’ - AGAGTTTGATCMTGGCTCAG - 3’);536R (5’ - GTATTACCGCGGCTGCTG - 3’)
characteristics.characteristic: pcr primers
characteristics.text: PRJEB7690
characteristics.characteristic: project name
characteristics.text: unidentified
characteristics.characteristic: scientific_name
characteristics.text: sediment
characteristics.characteristic: sediment environmental package
characteristics.text: pyrosequencing
characteristics.characteristic: sequencing method
characteristics.text: 16S rDNA
characteristics.characteristic: target gene
characteristics.text: V1-V3
characteristics.characteristic: target subfragment
characteristics.text: Storfjärden Spring 2009
characteristics.characteristic: title