characteristics.text: ERC000015
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-06-01T16:20:34Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2016-10-21T09:36:31Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA3158509
characteristics.characteristic: External Id
characteristics.text: CUSB-HM
characteristics.characteristic: INSDC center name
characteristics.text: 2015-06-01T16:20:34Z
characteristics.characteristic: INSDC first public
characteristics.text: 2016-10-21T09:36:31Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS620461
characteristics.characteristic: SRA accession
characteristics.text: AR1
characteristics.characteristic: Submitter Id
characteristics.text: 2009
characteristics.characteristic: collection date
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: The human gut microbiome and its associated mobilome is critical to health and wellbeing. It hosts a complex ecosystem comprising a multitude of bacterial species, which contributes functionality that would otherwise be absent from the host. Transient and commensal bacteria in the gut must withstand many stresses. The influence of mobile genetic elements, such as plasmids, in stress adaptation within the ecosystem is relatively unknown. We found evidence for plasmid mediated osmotolerance as a phenotype amongst the Proteobacteria in healthy faecal slurries. A transconjugant carrying multiple plasmids acquired from healthy faecal slurry demonstrated increased osmotolerance in the presence of metal salts, particularly potassium chloride, which was not evident in the recipient. Sequencing and analysis of the total plasmid DNA demonstrated the presence of plasmid-borne osmotolerance systems (including KdpD and H-NS) which may be linked to the observed phenotype. This is the first report of a transferable osmotolerance phenotype in gut commensals and may have implications for the transfer of osmotolerance in other niches.
characteristics.characteristic: description
characteristics.text: Human colon
characteristics.characteristic: environment (biome)
characteristics.text: Large intestine
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000059
characteristics.characteristic: environment (feature)
characteristics.text: feacal
characteristics.characteristic: environment (material)
characteristics.text: Ireland
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: 51.892993
characteristics.unit: DD
characteristics.characteristic: geographic location (latitude)
characteristics.text: -8.490510
characteristics.unit: DD
characteristics.characteristic: geographic location (longitude)
characteristics.text: human-gut
characteristics.characteristic: human gut environmental package
characteristics.text: metagenome
characteristics.characteristic: investigation type
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Investigation of the mobilome
characteristics.characteristic: project name
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: 454 pyrosequencing
characteristics.characteristic: sequencing method
characteristics.text: Metagenomic data from the mobilome
characteristics.characteristic: title