characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2016-08-09T17:01:30Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2016-03-17T08:09:44Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA3903135
characteristics.characteristic: External Id
characteristics.text: University of Cambridge
characteristics.characteristic: INSDC center name
characteristics.text: 2016-08-09T17:01:30Z
characteristics.characteristic: INSDC first public
characteristics.text: 2016-03-17T08:09:44Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1090269
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-4566:Sall41F5_48h_N2B27_1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: embryonic stem cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0002322
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: ES cells were cultured on 0.1% gelatin-coated plates in 2i/LIF conditions. For neural differentiation, ES cells were plated onto laminin-coated plates in N2B27 media at a density of 100,000 per 6-well. Medium was replaced every day and the cells were not passaged during the timecourse. RNA was extracted with TRI Reagent (Sigma). Libraries were prepared with the NEXTflex Rapid Directional Illumina RNA-seq Library Prep Kit (Bioo Scientific)
characteristics.characteristic: description
characteristics.text: Sall4/1 double knock out
characteristics.characteristic: genotype
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: Sall41F5_48h_N2B27_1
characteristics.characteristic: title