characteristics.text: ERC000022
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-08-28T17:01:20Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2016-10-21T09:33:16Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2730521
characteristics.characteristic: External Id
characteristics.text: INRA-CMSE
characteristics.characteristic: INSDC center name
characteristics.text: 2014-08-28T17:01:20Z
characteristics.characteristic: INSDC first public
characteristics.text: 2016-10-21T09:33:16Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS479682
characteristics.characteristic: SRA accession
characteristics.text: 101095C17
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/BTO_0004532
characteristics.characteristic: Submitter Id
characteristics.text: 2003
characteristics.characteristic: collection date
characteristics.text: 0.3
characteristics.unit: m
characteristics.characteristic: depth
characteristics.text: terrestrial biome
characteristics.characteristic: environment (biome)
characteristics.text: anthropogenic geographic feature
characteristics.characteristic: environment (feature)
characteristics.text: soil
characteristics.characteristic: environment (material)
characteristics.text: France
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: 267
characteristics.unit: m
characteristics.characteristic: geographic location (elevation)
characteristics.text: 46.9706095156
characteristics.unit: DD
characteristics.characteristic: geographic location (latitude)
characteristics.text: 3.7954295709
characteristics.unit: DD
characteristics.characteristic: geographic location (longitude)
characteristics.text: mimarks-survey
characteristics.characteristic: investigation type
characteristics.text: CATACTCTAC
characteristics.characteristic: multiplex identifiers
characteristics.text: soil metagenome
characteristics.characteristic: organism
characteristics.text: 5ng of DNA were used for a 25 µl PCR conducted under the following conditions: initial denaturation : 94degC_2 min; 35 cycles of denaturation : 94degC_30sec; annealing: 52degC_30sec; elongation: 72degC_1min; final elongation: 72degC_7min. A second PCR of 9 cycles was then conducted under similar PCR conditions with purified PCR products and ten base pair multiplex identifiers added to the primers at 5’ position to specifically identify each sample and avoid PCR biases
characteristics.characteristic: pcr conditions
characteristics.text: F479 (5’-CAGCMGCYGCNGTAANAC-3’) R888 (5’-CCGYCAATTCMTTTRAGT-3’)
characteristics.characteristic: pcr primers
characteristics.text: 4REG_bacteria_soil_diversity
characteristics.characteristic: project name
characteristics.text: soil metagenome
characteristics.characteristic: scientific_name
characteristics.text: 454-pyrosequencing
characteristics.characteristic: sequencing method
characteristics.text: soil
characteristics.characteristic: soil environmental package
characteristics.text: 16S rRNA gene
characteristics.characteristic: target gene
characteristics.text: Improving Soil Bacterial Taxa-Area Relationships Assessment Using DNA Meta-Barcoding
characteristics.characteristic: title