characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2020-06-01T04:05:06Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2016-03-03T14:09:41Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA3886630
characteristics.characteristic: External Id
characteristics.text: Brighton and Sussex Medical School
characteristics.characteristic: INSDC center name
characteristics.text: 2020-06-01T04:05:06Z
characteristics.characteristic: INSDC first public
characteristics.text: 2016-03-03T14:09:41Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1073764
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-4515:U1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: fruit fly
characteristics.characteristic: common name
characteristics.text: Protocols: For pcm[14] rescue males the UAS-pcm[WT] construct was first crossed into the pcm[14] stock to generate y[1] pcm[14]/FM7i-GFP; UAS-pcm[WT]/CyO-GFP stock. Virgin females from this stock were then crossed to males of arm-GAL4 driver. For wild-type E1-E3 is clean excision of the P element (Bloomington stock 11456) used to create pcm[14]. For wild-type G1-G3 is arm-Gal4 parent. For wild-type U1-U3 is UAS parent. 60 testes were dissected per biological replicate for wild-type samples. 120 testes were dissected per replicate for pcm[14] rescue sample. RNA was extracted using a Qiagen miRNeasy micro kit (217084) with on-column DNase treatment (79254) Sequencing libraries were created using an Illumina TruSeq RNA Sample Prep Kit v2
characteristics.characteristic: description
characteristics.text: 2-3 days old males
characteristics.characteristic: developmental stage
characteristics.text: wild-type
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genetic modification
characteristics.text: w[*]; UAS-pcm[WT]
characteristics.characteristic: genotype
characteristics.text: Drosophila melanogaster
characteristics.characteristic: organism
characteristics.text: Drosophila melanogaster
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7227
characteristics.characteristic: organism
characteristics.text: testis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000473
characteristics.characteristic: organism part
characteristics.text: Drosophila melanogaster
characteristics.characteristic: scientific_name
characteristics.text: U1
characteristics.characteristic: title