characteristics.text: ERC000013
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-07-22T17:15:39Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2016-10-21T09:38:56Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2395824
characteristics.characteristic: External Id
characteristics.text: ALFRED WEGENER INSTITUTE FOR POLAR AND MARINE RESE
characteristics.characteristic: INSDC center name
characteristics.text: 2014-07-22T17:15:39Z
characteristics.characteristic: INSDC first public
characteristics.text: 2016-10-21T09:38:56Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS419995
characteristics.characteristic: SRA accession
characteristics.text: 2111_NC
characteristics.characteristic: Submitter Id
characteristics.text: f: adapter A + key (4bp): CGTATCGCCTCCCTCGCGCCATCAG; r: adapter B + key (4bp): CTATGCGCCTTGCCAGCCCGCTCAG
characteristics.characteristic: adapters
characteristics.text: 2011
characteristics.characteristic: collection date
characteristics.text: Pacific oyster
characteristics.characteristic: common name
characteristics.text: blank sample
characteristics.characteristic: description
characteristics.text: aquatic
characteristics.characteristic: environment (biome)
characteristics.text: microbial
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/ENVO_01000008
characteristics.characteristic: environment (feature)
characteristics.text: water
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CHEBI_15377
characteristics.characteristic: environment (material)
characteristics.text: Germany
characteristics.characteristic: geographic location (countryand/orsea,region)
characteristics.text: 55.0288889
characteristics.unit: DD
characteristics.characteristic: geographic location (latitude)
characteristics.text: 8.4341667
characteristics.unit: DD
characteristics.characteristic: geographic location (longitude)
characteristics.text: host-associated
characteristics.characteristic: host-associated environmental package
characteristics.text: mimarks-survey
characteristics.characteristic: investigation type
characteristics.text: f: AGAGCGTCAC; r: ACGCTCGACA or f: AGCGACTAGC; r: ACGCTCGACA or f: ATAGATAGAC; r: ACGCTCGACA or f: ATATAGTCGC; r: ACGCTCGACA or f: CACGTGTCGC; r: ACGCTCGACA or f: CGAGACGCGC; r: ACGCTCGACA or f: CTAGTCACTC; r: ACGCTCGACA or f: AGAGCGTCAC; r: AGCACTGTAG or f: AGCGACTAGC; r: AGCACTGTAG or f: ATAGATAGAC; r: AGCACTGTAG or f: ATATAGTCGC; r: AGCACTGTAG or f: CACGTGTCGC; r: AGCACTGTAG or f: CGAGACGCGC; r: AGCACTGTAG or f: CTAGTCACTC; r: AGCACTGTAG
characteristics.characteristic: multiplex identifiers
characteristics.text: Crassostrea gigas
characteristics.characteristic: organism
characteristics.text: initial denaturation:94degC_1min; denaturation:94degC_40s;annealing:55degC_30s; elongation:72degC_30s; final elongation:72degC_2min; 35 cycles
characteristics.characteristic: pcr conditions
characteristics.text: f: linker (3bp) + 16S_338r: CATGCTGCCTCCCGTAGGAGT; r: linker (2bp) + 16S_27F: TCAGAGTTTGATCMTGGCTCAG
characteristics.characteristic: pcr primers
characteristics.text: PRJEB5702
characteristics.characteristic: project name
characteristics.text: Crassostrea gigas
characteristics.characteristic: scientific_name
characteristics.text: pyrosequencing
characteristics.characteristic: sequencing method
characteristics.text: 16S rRNA
characteristics.characteristic: target gene
characteristics.text: V1-V2
characteristics.characteristic: target subfragment
characteristics.text: Negative control
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0008460
characteristics.characteristic: title