characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2016-04-06T17:01:28Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2016-03-10T14:31:50Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA3892211
characteristics.characteristic: External Id
characteristics.text: Department of Cell and Molecular Biology, Karolinska Institutet
characteristics.characteristic: INSDC center name
characteristics.text: 2016-04-06T17:01:28Z
characteristics.characteristic: INSDC first public
characteristics.text: 2016-03-10T14:31:50Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1079345
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-39291457618732:E3.52.3434
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: 1525
characteristics.characteristic: cell
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Human embryos were obtained from two cohorts at the Huddinge Karolinska Hospital. The first cohort was from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5%O2 in IVF plates containing 700_l of CCMTM media (Vitrolife) covered with 300_l of OvoilTM (Vitrolife)). The second cohort was from frozen E2 embryos thawed (ThawKit� Cleave, VitroLife) and cultured in G-1� Plus media (VitroLife) until E3 and then transferred to CCM� media (VitroLife) from E3-E7. Embros were dissociated into single cells by the use of enzymatic (TrypLE) and mechanical separation, and then handpicked with fine glass capillaries by mouth pipetting. Single blastomemeres were directly dispensed into into individual wells of a 8-strip tube containing lysis buffer. Picelli et al, Full-length RNA-seq from single cells using Smart-seq2, Nature Protocols, 2014
characteristics.characteristic: description
characteristics.text: embryonic day 3
characteristics.characteristic: developmental stage
characteristics.text: E3.52
characteristics.characteristic: individual
characteristics.text: 2.149235134
characteristics.characteristic: inferred pseudo-time
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: embryo
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: OK
characteristics.characteristic: single cell well quality
characteristics.text: E3.52.3434
characteristics.characteristic: title
characteristics.text: No
characteristics.characteristic: treatment