characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2016-09-01T17:01:12Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2016-03-17T13:54:48Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA3903391
characteristics.characteristic: External Id
characteristics.text: Philipps University Marburg
characteristics.characteristic: INSDC center name
characteristics.text: 2016-09-01T17:01:12Z
characteristics.characteristic: INSDC first public
characteristics.text: 2016-03-17T13:54:48Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1090525
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-4577:Ecdysone_1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: S2
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001233
characteristics.characteristic: cell line
characteristics.text: fruit fly
characteristics.characteristic: common name
characteristics.text: Protocols: S2 cell lines were maintained under standard conditions. The conditions for S2 cell incubation for all experiments were set to six hours treatment with 1μM 20HE (in 5% EtOH). S2 nuclear and whole cell extracts were prepared and immunoprecipitated as described (Kunert et al., 2009; Meier et al., 2012). For immunoprecipitation of endogenous Mi-2, Protein G Sepharose was preincubated for 1hr with 1% fish skin gelatine and buffer D (0.2 mg/mL BSA in 20 mM Hepes KOH, pH 7.6 100 mM KCl 1.5 mM MgCl2, 0.2 mM EDTA, 20% (v/v) Glycerol, 0.1 mM DTT, protease inhibitors) at room temperature. Antibodies were incubated with pre-blocked Protein G sepharose for 1 hr at 4°C. Beads were washed twice with buffer D / 0.02% NP40 and transferred to low-binding tubes. 30ul of Protein G Sepharose were incubated with nuclear extract for 3 hrs at 4°C. The beads were washed five times with buffer D/0.02% NP40 and eluted with 40ul 2x SDS loading buffer. ChIP samples were sequenced on an Illumina IIx Genome Analyzer according to the manufacturer's instructions.
characteristics.characteristic: description
characteristics.text: rat monoclonal antidMi-2 (4D8)
characteristics.characteristic: immunoprecipitate
characteristics.text: Drosophila melanogaster
characteristics.characteristic: organism
characteristics.text: Drosophila melanogaster
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7227
characteristics.characteristic: organism
characteristics.text: Drosophila melanogaster
characteristics.characteristic: scientific_name
characteristics.text: Ecdysone_1
characteristics.characteristic: title