characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2016-03-23T17:02:39Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2016-01-26T12:06:15Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA3856689
characteristics.characteristic: External Id
characteristics.text: Wellcome Trust Sanger Institute, Cambridge, UK EMBL-EBI, Cambridge, UK
characteristics.characteristic: INSDC center name
characteristics.text: 2016-03-23T17:02:39Z
characteristics.characteristic: INSDC first public
characteristics.text: 2016-01-26T12:06:15Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1043823
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-3321:2cell_1_A
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Embryos were recovered from superovulated F1 (C57Bl6xCBA) mice into M2 media supplemented with 4% BSA, as described previously25. Individual blastomeres were collected at late 2-cell (48 hours after hCG), late 4-cell (10 hours after the first 2-cell blastomere divided), late 8-cell (10 hours after the first 4-cell blastomere divided), 16-cell (78 hours after hCG) and 32-cell (86 hours after hCG) stages. Embryos for blastomere isolation at the 2-, 4,- and 8-cell stages were all collected at the 2-cell stage and cultured in KSOM media (Millipore) at 37C with 5% CO2. Embryos for the 16- and 32-cell blastomeres were collected directly at the respective stages. The zona pellucida of individual embryos was removed using Tyrode's solution (Sigma). Zona-free embryos were incubated for 5 minutes (for the 2,4 and 8-cell stages) or 20 minutes (for the 16 and 32-cell stages) in Ca2+ and Mg2+ free M2 before disaggregation by careful pipetting with a flame-polished glass pipette. Each embryo was processed individually. Single blastomeres for all stages were placed into individual tubes containing 2.3 l of 0.2 % Triton X-100 (Sigma) supplemented with 1 U/l RNAsIN (Ambion). Multiplex sequencing libraries were generated from amplified cDNA using Nextera XT (Illumina) and sequenced on a HiSeq 2500 running in rapid mode.
characteristics.characteristic: description
characteristics.text: cleavage 2-cell
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001285
characteristics.characteristic: developmental stage
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: OK
characteristics.characteristic: single cell well quality
characteristics.text: 2cell_1_A
characteristics.characteristic: title