characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2017-12-14T17:02:56Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2016-02-29T14:27:21Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA3882068
characteristics.characteristic: External Id
characteristics.text: Department of Plant Biotechnology and Bioinformatics, Ghent University, Gent, Belgium Department of Plant Systems Biology,VIB, Gent, Belgium
characteristics.characteristic: INSDC center name
characteristics.text: 2017-12-14T17:02:56Z
characteristics.characteristic: INSDC first public
characteristics.text: 2016-02-29T14:27:21Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1069202
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-4508:Sha_T3
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: thale cress
characteristics.characteristic: common name
characteristics.text: Protocols: For RNA sequencing analysis, seedlings (12 DAS for Col-0 and Ey15-2 and 13 DAS for WalhaesB4, ICE97, ICE138, ICE75, Ler-0, Yeg-1, Sha, ICE153) were harvested in RNA ice-later solution (AM7030; Ambion) and incubated at -20°C for at least a week. Leaf 6 was micro-dissected on a cold plate with dry ice under a stereomicroscope, and frozen in liquid nitrogen. RNA was extracted with Trizol (Invitrogen) according to the manufacturer’s instructions and the RNeasy kit (Qiagen) with on-column DNase (Qiagen) digestion. RNA was quantified and the quality was checked with a 2100 Bioanalyzer (Agilent). Library preparation was done using the TruSeq RNA Sample Preparation Kit v2 (Illumina). In brief, polyA containing mRNA molecules are reverse transcribed, double-stranded cDNA is generated and adapters are ligated.
characteristics.characteristic: description
characteristics.text: Sha
characteristics.characteristic: ecotype
characteristics.text: GA20ox1 OE
characteristics.characteristic: genetic modification
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: organism
characteristics.text: Arabidopsis thaliana
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3702
characteristics.characteristic: organism
characteristics.text: Leaf 6
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: organism part
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: scientific_name
characteristics.text: Sha_T3
characteristics.characteristic: title