characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2017-08-01T17:01:47Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2016-02-23T14:25:19Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA3879131
characteristics.characteristic: External Id
characteristics.text: Computational Biology School of Life Sciences University of Dundee
characteristics.characteristic: INSDC center name
characteristics.text: 2017-08-01T17:01:47Z
characteristics.characteristic: INSDC first public
characteristics.text: 2016-02-23T14:25:19Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1066265
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-4501:hfq4
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: Protocols: Bacterial cultures, four independent cultures for each strain, were grown in liquid LB media (10 g/l tryptone, 5 g/l yeast extract, 5 g/l NaCl) with good aeration at 30C to mid log phase (5 h) RNA was isolated from bacterial cells by hot phenol extraction. Contaminating gDNA was removed from using the DNA-free Kit (Ambion), followed by ethanol precipitation of the RNA. Ribosomal RNA (rRNA) was depleted using the Ribo-zero rRNA Removal Kit for Gram-negative bacteria (Epicentre). Depleted RNA samples were purified by ethanol precipitation and resuspension in RNase free water, then subsequently purified with AMPure XP beads (Agencourt) to remove RNA fragments < 200bp. Illumina TruSeq
characteristics.characteristic: description
characteristics.text: gene knock out
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000506
characteristics.characteristic: genetic modification
characteristics.text: Hfq
characteristics.characteristic: genotype
characteristics.text: Serratia marcescens
characteristics.characteristic: organism
characteristics.text: Serratia marcescens
characteristics.characteristic: organism
characteristics.text: Serratia marcescens
characteristics.characteristic: scientific_name
characteristics.text: Db10
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002158
characteristics.characteristic: strain
characteristics.text: hfq4
characteristics.characteristic: title