characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2016-08-09T17:01:30Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2016-03-16T14:08:41Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA3902999
characteristics.characteristic: External Id
characteristics.text: University of Cambridge
characteristics.characteristic: INSDC center name
characteristics.text: 2016-08-09T17:01:30Z
characteristics.characteristic: INSDC first public
characteristics.text: 2016-03-16T14:08:41Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1090133
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-4565:Sall41_WT_Mi2B_2i_1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: cell line
characteristics.text: embryonic stem cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0002322
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: ES cells were cultured on 0.1% gelatin-coated plates in 2i/LIF conditions. Cells were fixed using 1% formaldehyde for 10 min at room temperature and fixation quenched with 150 mM glycine. Chromatin was sheared to an average fragment size between 200 and 300 bp using a Bioruptor sonication instrument (Diagenode). Immunoprecipitation was performed using antibodies against endogenous proteins or the FLAG tag. Libraries were prepared with the NEXTflex Rapid Illumina DNA-seq Library Prep Kit (Bioo Scientific).
characteristics.characteristic: description
characteristics.text: Sall4flox/floxSall1flox/flox
characteristics.characteristic: genotype
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: Sall41_WT_Mi2B_2i_1
characteristics.characteristic: title