characteristics.text: ERC000013
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-06-26T17:01:28Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2016-10-21T09:39:15Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA3359426
characteristics.characteristic: External Id
characteristics.text: SOUTHERNCROSSPLANTSCIENCE
characteristics.characteristic: INSDC center name
characteristics.text: 2015-06-26T17:01:28Z
characteristics.characteristic: INSDC first public
characteristics.text: 2016-10-21T09:39:15Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS712922
characteristics.characteristic: SRA accession
characteristics.text: Dicathais orbita1
characteristics.characteristic: Submitter Id
characteristics.text: 2015-04
characteristics.characteristic: collection date
characteristics.text: Dicathais orbita is a marine mollusc which is well known for the production of anticancer compounds that are precursors to Tyrian purple. This study aimed to assess the diversity and to identity bacteria associated with the Tyrian purple producing hypobranchial gland by comparison with foot tissues using a high-throughput sequencing approach. Total DNA was extracted from the hypobranchial gland and foot of D. orbita followed by high-throughput sequence, QIIME and MEGAN analysis of variable region V1-V3 of 16S rRNA bacterial gene amplicons. Metagenome analysis revealed a highly diverse bacterial assemblage associated with the hypobranchial gland and foot tissues of D. orbita. The dominant bacterial phylum in the metagenome data set was Proteobacteria followed by Tenericutes, Spirochaetes and Bacteroidetes. Larger numbers of bacterial taxa were present in the foot than the hypobranchial gland of D. orbita. This study revealed the high abundance of Vibrio spp. and the presence of unique bacterial communities in the hypobranchial gland which is also the site of indole production, bromoperoxidase activity and brominated secondary metabolites. This study also revealed the diversity of bacteria associated with the Tyrian Purple producing gland of a marine muricid and suggests bacteria have a potential role in the biosynthesis of Tyrian purple.
characteristics.characteristic: description
characteristics.text: coastal
characteristics.characteristic: environment (biome)
characteristics.text: Intertidal rocky areas
characteristics.characteristic: environment (feature)
characteristics.text: water
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CHEBI_15377
characteristics.characteristic: environment (material)
characteristics.text: Australia
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: 28 84
characteristics.unit: DD
characteristics.characteristic: geographic location (latitude)
characteristics.text: 153 60
characteristics.unit: DD
characteristics.characteristic: geographic location (longitude)
characteristics.text: host-associated
characteristics.characteristic: host-associated environmental package
characteristics.text: metagenome
characteristics.characteristic: investigation type
characteristics.text: Dicathais orbita
characteristics.characteristic: organism
characteristics.text: Metagenomic profiling of Dicathais orbita
characteristics.characteristic: project name
characteristics.text: Dicathais orbita
characteristics.characteristic: scientific_name
characteristics.text: Roche 454
characteristics.characteristic: sequencing method
characteristics.text: Metagenomic profiling of bacterial communities associated with Tyrian Purple production in a marine gastropod
characteristics.characteristic: title