characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2017-10-30T17:02:42Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2016-01-28T09:42:13Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA3859021
characteristics.characteristic: External Id
characteristics.text: University of Illinois at Urbana Champaign
characteristics.characteristic: INSDC center name
characteristics.text: 2017-10-30T17:02:42Z
characteristics.characteristic: INSDC first public
characteristics.text: 2016-01-28T09:42:13Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1046155
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-4357:OilHyp3
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Silastic tubing containing 35 ul of oil or 35ul of oil with 180ug/ml E2. Implants remained in mice for 7 days. After 7 days, mice were placed in a hypoxia chamber (BioSpherix, Lacona, NY) for 3 hours, which was equilibrated to 7% O2 and 93% N2. Mice and O2 concentrations were monitored continuously throughout the experiment. Mice were returned to normoxic conditions for 1 hour and then sacrificed. The brains were dissected and the cerebral cortices were harvested. Total RNA was extracted using Ambion RNAqueous according to manufacturer's protocol.Samples were treated with Turbo DNA-free reagent to remove genomic DNA. The TruSeq RNA sample prep kit was used according to manufacturer's protocol. 1ug total RNA was used to make poly-A selected and barcoded libraries. Libraries were pooled and quantifed using real time PCR and library quantification kit (Kapa Biosystems).
characteristics.characteristic: description
characteristics.text: hypoxia (7% O2) for 3 hours with 1 hour of recovery
characteristics.characteristic: growth condition
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: cerebral cortex
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000956
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: C57BL/6J
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000606
characteristics.characteristic: strain
characteristics.text: OilHyp3
characteristics.characteristic: title