characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2017-02-01T17:01:36Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2016-03-03T13:58:18Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA3886613
characteristics.characteristic: External Id
characteristics.text: Institute for Molecular Bioscience, University of Queenland, Brisbane, QLD 4072, Australia
characteristics.characteristic: INSDC center name
characteristics.text: 2017-02-01T17:01:36Z
characteristics.characteristic: INSDC first public
characteristics.text: 2016-03-03T13:58:18Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1073747
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-4511:Sox18 o.e. #1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: HUVEC
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002451
characteristics.characteristic: cell line
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: HUVECs were obtained from LONZA and grown according to suppliers recommendations. Cells were cultured in EBM2 media, supplemented with EGM2. Transfection (7 hours) was performed using Roche X-tremegene DNA transfection reagent in 0.4% FBS media. Directly following transfection, cells were stimulated (14 hours) with small molecule (or DMSO), in 0.4% FBS media. RNA was extracted using RNeasy mini kit (Qiagen). Samples (>100 ng RNA) were processed for whole transcriptome sequencing using TruSeq stranded total RNA library prep kit (Ribo‐Zero GOLD, Illumina).
characteristics.characteristic: description
characteristics.text: transfected with Sox18-cMyc
characteristics.characteristic: genotype
characteristics.text: EGM2 supplemented media + vascular endothelial growth factor A
characteristics.characteristic: growth condition
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Sox18 o.e. #1
characteristics.characteristic: title