characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2019-05-31T04:03:49Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2016-03-24T15:00:32Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA3907501
characteristics.characteristic: External Id
characteristics.text: Icahn School of Medicine at Mount Sinai
characteristics.characteristic: INSDC center name
characteristics.text: 2019-05-31T04:03:49Z
characteristics.characteristic: INSDC first public
characteristics.text: 2016-03-24T15:00:32Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1094635
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-4598:281_55.1
characteristics.characteristic: Submitter Id
characteristics.text: 55
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Mice carrying LoxP-site-flanked alleles of Traf2 were crossed to alfp-Cre transgenic mice to generate LPC-specific knockout mice (TRAF2^LPC-KO). Mice with combined conditional deletions of Traf2 and Caspase-8 (TRAF2/Casp-8^LPC-KO) or additional conditional deletion of Mlkl, Nemo or Ikkβ , or rather constitutive deletion of Ripk3 were generated by intercrossing the respective lines to TRAF2/Casp-8^LPC-KO mice. In all experiments, littermates carrying the respective loxP-flanked alleles but lacking expression of Cre recombinase were used as wild-type (WT) controls. Mice were bred on a mixed C57/BL6 - SV129Ola genetic background. Only sex- and age-matched animals were compared. Additional detail ara available on the corresponding paper. We performed RNA-seq (poly-A prepped libraries) on 15 fresh frozen samples (11 tumor tissue derived from 52-55 week aged TRAF2/Casp-8 mice, and 4 liver samples from wild-type mice). Starting material was 1 μg of total RNA extracted using RNeasy Mini Kit (Qiagen). RNA integrity was checked by the 2100 Bioanalyzer using the RNA 6000 Nano assay (Agilent, CA, USA). All processed total RNA samples had RIN value of 7.0 or greater. The sequencing library was prepared with the standard TruSeq RNA Sample Prep Kit v2 protocol.
characteristics.characteristic: description
characteristics.text: yes
characteristics.characteristic: disease
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: obsolete_sex
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: 281_55.1
characteristics.characteristic: title