characteristics.text: ERC000013
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2017-03-09T17:01:56Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2016-10-21T09:40:06Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA3873112
characteristics.characteristic: External Id
characteristics.text: INRA-CMSE
characteristics.characteristic: INSDC center name
characteristics.text: 2017-03-09T17:01:56Z
characteristics.characteristic: INSDC first public
characteristics.text: 2016-10-21T09:40:06Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1060246
characteristics.characteristic: SRA accession
characteristics.text: Delia_Ile_02
characteristics.characteristic: Submitter Id
characteristics.text: 2009
characteristics.characteristic: collection date
characteristics.text: Delia radicum pupae were sampled in broccoli fields in Brittany (Western France) between April and June 2009 from three distinct areas spanning approximately 200km, which are representative of brassicaceous production: 'Finistere' (westernmost tip of Brittany), 'Cotes d'Armor' (central Brittany) and 'Ille et Vilaine' (easternmost Brittany). For convenience, these populations will therefore be referred to as 'western', 'central' and 'eastern'. Pupae were stored individually in punctured Eppendorf tubes in controlled conditions (20degC, 60% RH and a 16-L: 8-D photoperiod) until emergence of adults. Individuals of the four species (D. radicum and its three parasitoids A. bilineata, A. bipustulata and T. rapae) emerging from the pupae were then collected and stored in 95% ethanol (A. bilineata) or reared in the laboratory between 6 and 18 months in the same controlled conditions as above and then stored in 95% ethanol (other species). In order to study most of the extensive microbiota that individuals of each species could harbor, we took individuals at the adult stage in laboratory populations. No specific permissions were required for these collection activities and samplings did not involve endangered or protected species.
characteristics.characteristic: description
characteristics.text: environmental system
characteristics.characteristic: environment (biome)
characteristics.text: organic feature
characteristics.characteristic: environment (feature)
characteristics.text: organic material
characteristics.characteristic: environment (material)
characteristics.text: France
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: 48,1
characteristics.unit: DD
characteristics.characteristic: geographic location (latitude)
characteristics.text: -3.9833333333333334
characteristics.unit: DD
characteristics.characteristic: geographic location (longitude)
characteristics.text: Delia radicum
characteristics.characteristic: host common name
characteristics.text: host-associated
characteristics.characteristic: host-associated environmental package
characteristics.text: mimarks-survey
characteristics.characteristic: investigation type
characteristics.text: TACTG
characteristics.characteristic: multiplex identifiers
characteristics.text: 2
characteristics.characteristic: number of the replicate
characteristics.text: insect metagenome
characteristics.characteristic: organism
characteristics.text: Fwd:5-GTGCCAGCMGCCGCGGTAATAC-3; Rev:3-CCGTCAATTCCTTTGAGTTT-5
characteristics.characteristic: pcr primers
characteristics.text: Delia-Radicum_parasitoid_Bacteria
characteristics.characteristic: project name
characteristics.text: insect metagenome
characteristics.characteristic: scientific_name
characteristics.text: 454-Pyrosequencing
characteristics.characteristic: sequencing method
characteristics.text: 16S rDNA
characteristics.characteristic: target gene
characteristics.text: Delia-Radicum_parasitoid_Bacteria_2016
characteristics.characteristic: title