characteristics.text: ERC000025
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2016-01-14T17:03:09Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2016-10-21T09:43:22Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA3256107
characteristics.characteristic: External Id
characteristics.text: ALFRED WEGENER INSTITUTE FOR POLAR AND MARINE RESE
characteristics.characteristic: INSDC center name
characteristics.text: 2016-01-14T17:03:09Z
characteristics.characteristic: INSDC first public
characteristics.text: 2016-10-21T09:43:22Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS661086
characteristics.characteristic: SRA accession
characteristics.text: DNA_extract_NTC_IC2012
characteristics.characteristic: Submitter Id
characteristics.text: 2012-06-10
characteristics.characteristic: collection date
characteristics.text: Blank extraction with ddH2O; this was a single NTC with amplificiation
characteristics.characteristic: description
characteristics.text: water
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CHEBI_15377
characteristics.characteristic: environment (biome)
characteristics.text: water
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CHEBI_15377
characteristics.characteristic: environment (feature)
characteristics.text: water
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CHEBI_15377
characteristics.characteristic: environment (material)
characteristics.text: Germany
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: 55.028
characteristics.unit: DD
characteristics.characteristic: geographic location (latitude)
characteristics.text: 8.434
characteristics.unit: DD
characteristics.characteristic: geographic location (longitude)
characteristics.text: mimarks-survey
characteristics.characteristic: investigation type
characteristics.text: miscellaneous natural or artificial environment
characteristics.characteristic: miscellaneous environmental package
characteristics.text: f: TGTCCAGA; r TGTGGACT
characteristics.characteristic: multiplex identifiers
characteristics.text: metagenome
characteristics.characteristic: organism
characteristics.text: initial denaturation:98degC_30s; denaturation:98degC_9s;annealing:55degC_1min; elongation:72degC_90s; final elongation:72degC_10min; 30 cycles
characteristics.characteristic: pcr conditions
characteristics.text: 27f-338r
characteristics.characteristic: pcr primers
characteristics.text: PRJEB8492
characteristics.characteristic: project name
characteristics.text: metagenome
characteristics.characteristic: scientific_name
characteristics.text: Sequencing by synthesis Illumina paired-end
characteristics.characteristic: sequencing method
characteristics.text: 16s rRNA
characteristics.characteristic: target gene
characteristics.text: V1-V2
characteristics.characteristic: target subfragment
characteristics.text: DNA blank extraction
characteristics.characteristic: title