characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2016-06-08T17:01:37Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2016-03-17T13:21:32Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA3903315
characteristics.characteristic: External Id
characteristics.text: Center of Biomedical Research (CIBM) University of Granada
characteristics.characteristic: INSDC center name
characteristics.text: 2016-06-08T17:01:37Z
characteristics.characteristic: INSDC first public
characteristics.text: 2016-03-17T13:21:32Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1090449
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-4572:Control-TX-D12
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Previously generated Sox9f/f; Sox8-/- mice were bred to Wt1-CreERT2 mice and the resulting double heterozygous offspring harboring the Cre allele was backcrossed to Sox9f/f; Sox8-/- mice to obtain heterozygous and homozygous compound Sox9; Sox8 conditional mutants. The same mating scheme was followed with the Sox9-CreERT2 mouse line. To report CRE activity, an R26R-EYFP reporter allele was crossed into Wt1-CreERT2; Sox9f/f; Sox8-/- and Sox9-CreERT2; Sox9f/f; Sox8-/- mice. Mice were feeded with a Tamoxifen (TX) supplemented diet (40mg TX/100g Harlan 2914 diet) for one month. TX administration were started at 2 months (P60). Both testes were extracted from six 5 months old, TX-treated mutant males (three Wt1- CreERT2; Sox9f/f; Sox8-/- and three Sox9-CreERT2; Sox9f/f; Sox8-/-). As controls, both gonads were also extracted from four 5 months old control male mice (two TX-treated and two untreated) as well as from two 4-5 months old normal females. All TX-treated mice were euthanized three months after the initiation of diet TX-treatment for one month. The two gonads of each individual were pooled, homogenized in 1ml of RNAzol (Molecular Research Center, Inc.) per 100mg of tissue and the total RNAs were then individually purified from the twelve samples following the RNAzol manufacturer’s instructions. After successfully passing Macrogen Inc. quality check, the twelve RNAs were paired-end sequenced
characteristics.characteristic: description
characteristics.text: Tamoxifen treated control male
characteristics.characteristic: genetic modification
characteristics.text: Sox9+/+;Sox8-/-
characteristics.characteristic: genotype
characteristics.text: Male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: obsolete_sex
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: testis
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: Control-TX-D12
characteristics.characteristic: title