characteristics.text: ERC000013
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-07-22T17:15:39Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2016-10-21T09:38:56Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2670305
characteristics.characteristic: External Id
characteristics.text: ALFRED WEGENER INSTITUTE FOR POLAR AND MARINE RESE
characteristics.characteristic: INSDC center name
characteristics.text: 2014-07-22T17:15:39Z
characteristics.characteristic: INSDC first public
characteristics.text: 2016-10-21T09:38:56Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS421555
characteristics.characteristic: SRA accession
characteristics.text: 2311_41
characteristics.characteristic: Submitter Id
characteristics.text: f: adapter A + key (4bp): CGTATCGCCTCCCTCGCGCCATCAG; r: adapter B + key (4bp): CTATGCGCCTTGCCAGCCCGCTCAG
characteristics.characteristic: adapters
characteristics.text: 0.0002
characteristics.unit: L
characteristics.characteristic: amount or size of sample collected
characteristics.text: hemolymph
characteristics.characteristic: body habitat
characteristics.text: hemolymph
characteristics.characteristic: body product
characteristics.text: adductor muscle
characteristics.characteristic: body site
characteristics.text: 2010-11-23
characteristics.characteristic: collection date
characteristics.text: Pacific oyster
characteristics.characteristic: common name
characteristics.text: Experimental sample. Sample title code: [original temperature][experimental temperature]_[infection]_[time point]. For details see study description and 'perturbation' and 'detailed conditions' fields
characteristics.characteristic: description
characteristics.text: 7 days after transfer from flowthrough to stationary seawater aquarium, temperature change from 8°C to 22°C and injection with soya-peptone medium
characteristics.characteristic: detailed conditions
characteristics.text: healthy
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000461
characteristics.characteristic: disease status
characteristics.text: aquatic, marine
characteristics.characteristic: environment (biome)
characteristics.text: organic
characteristics.characteristic: environment (feature)
characteristics.text: organic material, bodily fluid
characteristics.characteristic: environment (material)
characteristics.text: Germany
characteristics.characteristic: geographic location (countryand/orsea,region)
characteristics.text: 55.0288889
characteristics.unit: DD
characteristics.characteristic: geographic location (latitude)
characteristics.text: 8.4341667
characteristics.unit: DD
characteristics.characteristic: geographic location (longitude)
characteristics.text: 22
characteristics.unit: ºC
characteristics.characteristic: host body temperature
characteristics.text: Pacific oyster
characteristics.characteristic: host common name
characteristics.text: 41
characteristics.characteristic: host subject id
characteristics.text: 29159
characteristics.characteristic: host taxid
characteristics.text: host-associated
characteristics.characteristic: host-associated environmental package
characteristics.text: mimarks-survey
characteristics.characteristic: investigation type
characteristics.text: adult
characteristics.characteristic: life stage
characteristics.text: Schleswig-Holstein, Wadden Sea (North Sea), island of Sylt, Kongishafen
characteristics.characteristic: location details
characteristics.text: f: CATACTCTAC; r: AGACGCACTC or f: CATACTCTAC; r: ATATCGCGAG
characteristics.characteristic: multiplex identifiers
characteristics.text: Crassostrea gigas
characteristics.characteristic: organism
characteristics.text: initial denaturation:94degC_1min; denaturation:94degC_40s;annealing:55degC_30s; elongation:72degC_30s; final elongation:72degC_2min; 35 cycles
characteristics.characteristic: pcr conditions
characteristics.text: f: linker (3bp) + 16S_338r: CATGCTGCCTCCCGTAGGAGT; r: linker (2bp) + 16S_27F: TCAGAGTTTGATCMTGGCTCAG
characteristics.characteristic: pcr primers
characteristics.text: temperature stressed, mock-infected
characteristics.characteristic: perturbation
characteristics.text: PRJEB5702
characteristics.characteristic: project name
characteristics.text: syringe
characteristics.characteristic: sample collection device or method
characteristics.text: Crassostrea gigas
characteristics.characteristic: scientific_name
characteristics.text: pyrosequencing
characteristics.characteristic: sequencing method
characteristics.text: seawater (local, supplied through cca 200 m long pipe, 30 um filtered and protein skimmed)
characteristics.characteristic: substrate
characteristics.text: 16S rRNA
characteristics.characteristic: target gene
characteristics.text: V1-V2
characteristics.characteristic: target subfragment
characteristics.text: 22
characteristics.unit: ºC
characteristics.characteristic: temperature
characteristics.text: C.gigas hemolymph microbiota CW_0_7
characteristics.characteristic: title