characteristics.text: 170
characteristics.characteristic: DNA insert size
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2016-06-01T17:01:04Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2016-03-04T10:48:37Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA3888314
characteristics.characteristic: External Id
characteristics.text: Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH
characteristics.characteristic: INSDC center name
characteristics.text: 2016-06-01T17:01:04Z
characteristics.characteristic: INSDC first public
characteristics.text: 2016-03-04T10:48:37Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1075448
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-4527:Sample 1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: HG-3
characteristics.characteristic: cell line
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Fragmentation of 100 ng purified genomic DNA was done on Covaris S2 and the procedure adjusted to obtain fragments with a peak length of 130 and 170 bp. Fragmentation of 100 ng purified genomic DNA (gDNA) in 55 μl Tris-EDTA buffer was done on Covaris S2 and the procedure adjusted to obtain fragments with a peak length of 130 and 170 bp, respectively. After library preparation from 100 ng of fragmented gDNA using Agilent SureSelectXT v5 (50 Mb) and v5+UTR (75 Mb), libraries were purified, size validated and prepared for sequencing according to the manufacturer's protocols. Libraries were sequenced on Illumina HiSeq2500 using TruSeq SBS Kit v3-HS (2x101, paired-end run).
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Sample 1
characteristics.characteristic: title