characteristics.text: ERC000022
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2016-10-30T17:09:20Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2016-10-21T09:34:05Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA3598039
characteristics.characteristic: External Id
characteristics.text: Thuenen Institute of Biodiversity
characteristics.characteristic: INSDC center name
characteristics.text: 2016-10-30T17:09:20Z
characteristics.characteristic: INSDC first public
characteristics.text: 2016-10-21T09:34:05Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS905188
characteristics.characteristic: SRA accession
characteristics.text: S46
characteristics.characteristic: Submitter Id
characteristics.text: 2011-08
characteristics.characteristic: collection date
characteristics.text: Arable field
characteristics.characteristic: current land use
characteristics.text: grass-clover mixture: Medicago sativa, Trifolium hybridum, Lotus corniculatus, Lolium perenne, Festuca pratense, Phleum pratense
characteristics.characteristic: current vegetation
characteristics.text: 0.18
characteristics.unit: m
characteristics.characteristic: depth
characteristics.text: Mock community - Replicate 1
characteristics.characteristic: description
characteristics.text: Temporate broadleaf forest biome
characteristics.characteristic: environment (biome)
characteristics.text: Arable filed
characteristics.characteristic: environment (feature)
characteristics.text: Arable soil
characteristics.characteristic: environment (material)
characteristics.text: Denmark
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: 63
characteristics.unit: m
characteristics.characteristic: geographic location (elevation)
characteristics.text: +55.47
characteristics.unit: DD
characteristics.characteristic: geographic location (latitude)
characteristics.text: +07.12
characteristics.unit: DD
characteristics.characteristic: geographic location (longitude)
characteristics.text: fertilisation 18 months before sampling; NPK: 100 kg total-N ha-1, 19 kg P ha-1 and 87 kg K ha-1; AM: 37.5 t wet weight cattle slurry ha-1
characteristics.characteristic: history/agrochemical additions
characteristics.text: Winter wheat, beet roots, barley, grass-clover mixture
characteristics.characteristic: history/crop rotation
characteristics.text: A horizon
characteristics.characteristic: horizon
characteristics.text: metagenome
characteristics.characteristic: investigation type
characteristics.text: 862
characteristics.unit: mm
characteristics.characteristic: mean annual and seasonal precipitation
characteristics.text: 7.7
characteristics.unit: ºC
characteristics.characteristic: mean annual and seasonal temperature
characteristics.text: FastDNA™ SPIN Kit for Soil (MP Biomedicals, Santa Ana, CA)
characteristics.characteristic: nucleic acid extraction
characteristics.text: metagenome
characteristics.characteristic: organism
characteristics.text: 6.3
characteristics.characteristic: ph
characteristics.text: CaCl2
characteristics.characteristic: ph method
characteristics.text: SPP 1315 Biogeochemical Interfaces in Soil
characteristics.characteristic: project name
characteristics.text: mild sonication, wet sieving, centrifugation (see http://dx.doi.org/10.1016/j.soilbio.2015.08.018)
characteristics.characteristic: sample material processing
characteristics.text: metagenome
characteristics.characteristic: scientific_name
characteristics.text: Illumina MiSeq, dual-index paried-end multiplexing
characteristics.characteristic: sequencing method
characteristics.text: soil
characteristics.characteristic: soil environmental package
characteristics.text: Typic Hapludalf
characteristics.characteristic: soil taxonomic/fao classification
characteristics.text: 50-55% water holding capacity, 4°C, darkness
characteristics.characteristic: storage conditions (fresh/frozen/other)
characteristics.text: 16S rRNA gene
characteristics.characteristic: target gene
characteristics.text: V4
characteristics.characteristic: target subfragment
characteristics.text: 64/26/10
characteristics.unit: % sand/silt/clay
characteristics.characteristic: texture
characteristics.text: Soil particle size fractions
characteristics.characteristic: title