characteristics.text: ERC000028
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-02-12T17:06:47Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2016-10-21T09:44:55Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2757648
characteristics.characteristic: External Id
characteristics.text: PASTEUR
characteristics.characteristic: INSDC center name
characteristics.text: 2015-02-12T17:06:47Z
characteristics.characteristic: INSDC first public
characteristics.text: 2016-10-21T09:44:55Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS538967
characteristics.characteristic: SRA accession
characteristics.text: phiCD2111
characteristics.characteristic: Submitter Id
characteristics.text: 2012
characteristics.characteristic: collection date
characteristics.text: Clostridium difficile ATCC9689 strain has been investigated for the presence of functional prophages. Following mitomycine C induction, a temperate phage termed φCD211 has been isolated. Transmission electron microscopy (TEM) has revealed a member of Siphoviridae family, with large icosahedral capsid and unusually long tail. Phage DNA has been purified directly from induction lysate and subjected to complete genome sequencing using Illumina technology. Following sequencing, double stranded-DNA (dsDNA) genome of 131,326 bp has been assembled and a putative function could be assigned to 60 of the 190 open reading frames. LC-MS/MS analysis of whole virion particles has enabled identification of 33 putative structural proteins. Bioinformatics analysis revealed the presence of multiple transposases along with putative horizontally acquired regions of bacterial origin. Additionally, an unusually high number (15) of transcriptional regulators / DNA binding proteins have been identified suggesting a complex regulation of DNA replication and gene transcription. To the best of our knowledge, φCD211 genome is the largest phage genomic sequence described so far.
characteristics.characteristic: description
characteristics.text: Canada
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: disease
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000408
characteristics.characteristic: host health state
characteristics.text: Peptoclostridium difficile
characteristics.characteristic: host scientific name
characteristics.text: laboratory
characteristics.characteristic: isolation source
characteristics.text: Clostridium phage phiCD211
characteristics.characteristic: organism
characteristics.text: Clostridium phage phiCD211
characteristics.characteristic: scientific_name
characteristics.text: ATCC9689
characteristics.characteristic: serovar
characteristics.text: Clostridium difficile
characteristics.characteristic: strain
characteristics.text: Whole Genome Sequence and Molecular Characterization of a Giant Temperate Phage Infecting Clostridium difficile
characteristics.characteristic: title