characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2016-08-01T17:01:06Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2016-01-25T17:33:10Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA3856266
characteristics.characteristic: External Id
characteristics.text: Medical University of Vienna
characteristics.characteristic: INSDC center name
characteristics.text: 2016-08-01T17:01:06Z
characteristics.characteristic: INSDC first public
characteristics.text: 2016-01-25T17:33:10Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1043400
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-4341:P19_CP
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Mononuclear cells were isolated from peripheral blood or bone marrow using Ficoll. Genomic DNA (gDNA) was isolated from MNCs using QIAsymphony DSP DNA Midi Kit (Qiagen), MspI (New England Biolabs, NEB) digested, end-repaired and A-tailed using Klenow Polymerase (NEB) and dATP, dCTP and dGTP. Illumina adapters were ligated using Quick Ligase (NEB) followed by AMPure XP bead size selection (Beckman Coulter). RRBS libraries were bisulfite treated using EZ DNA MethylationDirect kit (Zymo Research) followed by quantification by qPCR. Enrichment PCR was performed using PfuTurbo Cx Hotstart kit (Agilent Technologies) followed by AMPure XP bead clean up. Quality of final libraries was checked by Experion analysis (BioRad).
characteristics.characteristic: description
characteristics.text: chronic myeloid leukemia
characteristics.characteristic: disease
characteristics.text: chronic phase chronic myeloid leukemia
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000339
characteristics.characteristic: disease staging
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: P19_CP
characteristics.characteristic: title