characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2017-08-25T17:01:55Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2016-02-02T16:24:10Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA3864091
characteristics.characteristic: External Id
characteristics.text: Institute of Environmental Medicine Karolinska Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2017-08-25T17:01:55Z
characteristics.characteristic: INSDC first public
characteristics.text: 2016-02-02T16:24:10Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1051225
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-4398:Sample 4
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: C17.2
characteristics.characteristic: cell line
characteristics.text: neuronal stem cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000047
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: For differentiation experiments the cells were seeded at a density of 2000 cells/cm2 in suitable cell culture plates in DMEM complete cell medium. After 24 h the cell medium was replaced with differentiation medium consisting of DMEM:F12 medium (Gibco) supplemented with 2 mM glutamine (Gibco), 100 U penicillin/mL, 100 U streptomycin/mL (Gibco) and N2 supplement (1:100) (ThermoFischer Scientific). Half of this differentiation medium was changed every 3 days during differentiation. For RNA extraction, the cells were washed after exposure and total RNA was extracted using the RNeasy Mini Columns (Qiagen) according to the manufacturers instructions, including the purification step with DNase I treatment. Stranded mRNA-seq (Illumina TruSeq RNA HT with polyA-selection
characteristics.characteristic: description
characteristics.text: 1 day differentiated
characteristics.characteristic: developmental stage
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: Sample 4
characteristics.characteristic: title