characteristics.text: ERC000022
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2013-12-12T11:33:51Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2016-10-21T09:33:03Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2275897
characteristics.characteristic: External Id
characteristics.text: UH
characteristics.characteristic: INSDC center name
characteristics.text: 2013-12-12T11:33:51Z
characteristics.characteristic: INSDC first public
characteristics.text: 2016-10-21T09:33:03Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS383292
characteristics.characteristic: SRA accession
characteristics.text: 7
characteristics.characteristic: Submitter Id
characteristics.text: 2
characteristics.unit: kg
characteristics.characteristic: amount or size of sample collected
characteristics.text: 2009-10-01
characteristics.characteristic: collection date
characteristics.text: composite of 10 soil cores, sieving <5 mm
characteristics.characteristic: composite design/sieving (if any)
characteristics.text: landfarming, no more dumping
characteristics.characteristic: current land use
characteristics.text: 0.61
characteristics.unit: m
characteristics.characteristic: depth
characteristics.text: Subsurface soil, total petroleum hydrocarbons 44 g/kg, bacterial clones
characteristics.characteristic: description
characteristics.text: landfarming field
characteristics.characteristic: environment (biome)
characteristics.text: refinery waste landfarming field
characteristics.characteristic: environment (feature)
characteristics.text: soil
characteristics.characteristic: environment (material)
characteristics.text: THP 44 g/kg
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001253
characteristics.characteristic: experimental factor
characteristics.text: Finland
characteristics.characteristic: geographic location (countryand/orsea,region)
characteristics.text: 20
characteristics.unit: m
characteristics.characteristic: geographic location (elevation)
characteristics.text: 60.303
characteristics.unit: DD
characteristics.characteristic: geographic location (latitude)
characteristics.text: 25.537
characteristics.unit: DD
characteristics.characteristic: geographic location (longitude)
characteristics.text: urea fertilisation, liming
characteristics.characteristic: history/agrochemical additions
characteristics.text: spreading of oily sludges 1980-2005
characteristics.characteristic: history/previous land use
characteristics.text: mimarks-survey
characteristics.characteristic: investigation type
characteristics.text: TOPO-TA Cloning, Invitrogen
characteristics.characteristic: library construction method
characteristics.text: by amplicon length, few of each OTUs sequenced
characteristics.characteristic: library screening strategy
characteristics.text: MOBIO RNA Power Soil Total RNA Isolation Kit+RNA Power Soil DNA Elution Accessory Kit
characteristics.characteristic: nucleic acid extraction
characteristics.text: uncultured bacterium
characteristics.characteristic: organism
characteristics.text: fD1 (AGAGTTTGATCCTGGCTCAG), PRUN518r (ATTACCGCGGCTGCTGG)
characteristics.characteristic: pcr primers
characteristics.text: 6.9
characteristics.characteristic: ph
characteristics.text: 0.02 M CaCl2
characteristics.characteristic: ph method
characteristics.text: duplicates pooled
characteristics.characteristic: pooling of DNA extracts (ifdone)
characteristics.text: Oilfield
characteristics.characteristic: project name
characteristics.text: 1
characteristics.unit: g
characteristics.characteristic: sample weight for DNA extraction
characteristics.text: uncultured bacterium
characteristics.characteristic: scientific_name
characteristics.text: manual
characteristics.characteristic: sequence quality check
characteristics.text: Sanger (both strands)
characteristics.characteristic: sequencing method
characteristics.text: soil
characteristics.characteristic: soil environmental package
characteristics.text: 16S rRNA
characteristics.characteristic: target gene
characteristics.text: V1-V3
characteristics.characteristic: target subfragment
characteristics.text: PitD_Bacteria
characteristics.characteristic: title
characteristics.text: VarioMax CN
characteristics.characteristic: total N method
characteristics.text: 3.0
characteristics.unit: g/kg
characteristics.characteristic: total n
characteristics.text: VarioMax CN
characteristics.characteristic: total organic C method
characteristics.text: 142
characteristics.unit: g/kg
characteristics.characteristic: total organic carbon
characteristics.text: 0.03
characteristics.unit: g/g
characteristics.characteristic: water content of soil