characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2017-09-05T17:02:02Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2016-01-28T15:35:13Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA3860151
characteristics.characteristic: External Id
characteristics.text: Laboratory of Molecular Medicine and Genomics Department of Medicine and Surgery University of Salerno
characteristics.characteristic: INSDC center name
characteristics.text: 2017-09-05T17:02:02Z
characteristics.characteristic: INSDC first public
characteristics.text: 2016-01-28T15:35:13Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1047285
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-4368:Sample 1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: MCF-7
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001203
characteristics.characteristic: cell line
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: RNA was extracted from cells expressing or not ER using the standard RNA extraction method with TRIzol (Life Technologies) After Ago2 knock-down in C-TAP-ER and MCF7 cells starved for 5 days, nascent RNA was extracted from each sample following the procedure developed by Khodor et al.28. Nascent RNA isolation was also conducted on non-transfected and transfected with pLKO.1-puro Non-Target shRNA Control Plasmid DNA (Sigma-Aldrich) cells as control. TRIzol reagent (Life Techonolgies) was added, an incubation at 65C was performed to dissolve DNA-Histone-Pol II-RNA pellets28 and RNA was extracted following the manufacturers protocol. For sequencing, indexed libraries were prepared using 1 g of Nascent RNA as starting material, with TruSeq Stranded Total RNA Sample Prep Kit (Illumina Inc.)
characteristics.characteristic: description
characteristics.text: expression of estrogen receptor fused to C terminus tandem affinity purification tag
characteristics.characteristic: genotype
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: expression of estrogen receptor
characteristics.characteristic: phenotype
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Sample 1
characteristics.characteristic: title