characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2017-08-25T17:01:55Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2016-02-02T16:24:09Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA3864058
characteristics.characteristic: External Id
characteristics.text: Institute of Environmental Medicine Karolinska Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2017-08-25T17:01:55Z
characteristics.characteristic: INSDC first public
characteristics.text: 2016-02-02T16:24:09Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1051192
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-4398:Sample 1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: C17.2
characteristics.characteristic: cell line
characteristics.text: neuronal stem cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000047
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Under proliferating conditions the C17.2 cells were grown in cell culture dishes in DMEM high glucose, GlutaMAX (Gibco) supplemented with 5% horse serum, heat inactivated (Gibco) and 10% fetal bovine serum, heat inactivated (Gibco), 2 mM glutamine (Gibco), 100 U penicillin/mL, and 100 U streptomycin/mL (Gibco). The cells were split every 3-4 days. During this proliferating phase samples were harvested for RNAseq. For RNA extraction, the cells were washed after exposure and total RNA was extracted using the RNeasy Mini Columns (Qiagen) according to the manufacturers instructions, including the purification step with DNase I treatment. Stranded mRNA-seq (Illumina TruSeq RNA HT with polyA-selection
characteristics.characteristic: description
characteristics.text: undifferentiated
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000621
characteristics.characteristic: developmental stage
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: Sample 1
characteristics.characteristic: title