characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2017-07-17T17:01:33Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-03-13T11:57:38Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA103910408
characteristics.characteristic: External Id
characteristics.text: University of Cambridge
characteristics.characteristic: INSDC center name
characteristics.text: 2017-07-17T17:01:33Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-03-13T11:57:38Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1599576
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-5577:KDPat1b_Rep2_R1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: HEK293T
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001184
characteristics.characteristic: cell line
characteristics.text: epithelial cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000066
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: HEK293T cells were maintained in DMEM supplemented with 10% fetal bovine serum. Transfection of siRNA was performed using Lipofectamine 2000, according to manufacturer’s instructions. On day 2, the medium was replaced and the second siRNA treatment was performed. On day 3 the medium was replaced . Cells were harvested on day 4. RNA was extracted with TriReagent (Sigma) following manufacturer’s instructions. Ribo-Zero TruSeq stranded mRNA libraries were prepared for each sample.
characteristics.characteristic: description
characteristics.text: embryo
characteristics.characteristic: developmental stage
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: kidney
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: KDPat1b_Rep2_R1
characteristics.characteristic: title