characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2018-04-24T17:03:46Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-03-06T12:10:52Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA103891180
characteristics.characteristic: External Id
characteristics.text: Instituto Gulbenkian de Ciencia
characteristics.characteristic: INSDC center name
characteristics.text: 2018-04-24T17:03:46Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-03-06T12:10:52Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1580348
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-5541:Zeb1_Cb192
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: stem cell derived cell line
characteristics.characteristic: cell line
characteristics.text: neural stem cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000047
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Cells were cultured as previously described (Conti et al. 2005), using laminin as a media supplement (20ug/mL), and plated at a density of 28,000/cm2 Cells were fixed sequentially with di(N-succimidyl) glutarate and 1% formaldehyde in phosphate buffered saline and then lysed, sonicated and immunoprecipitated, as previously described (Castro et al., 2011), with an anti-ZEB1 antibody DNA libraries were prepared from 10ng of immunoprecipitated DNA according to the standard Illumina ChIP-seq protocol.
characteristics.characteristic: description
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: media
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000579
characteristics.characteristic: growth condition
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Zeb1_Cb192
characteristics.characteristic: title