characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2017-05-01T17:02:13Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-03-30T14:01:41Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA103951537
characteristics.characteristic: External Id
characteristics.text: Bielefeld University, Center for Biotechnology (CeBiTec), Microbial Genomics and Biotechnology, Bielefeld, Germany
characteristics.characteristic: INSDC center name
characteristics.text: 2017-05-01T17:02:13Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-03-30T14:01:41Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1640705
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-5623:S_lividans_TK24_Syscomp_light_Dark_strRNA-S2-DARK
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: Protocols: Main cultures were performed in 48 well MTP 48 B “FlowerPlates®” covered by a F GP 10 gas-permeable sealing foil (m2p labs GmbH, Baesweiler, Germany). Per well, 1000 µL medium was inoculated to an initial OD600 of 0.2. Cultivation conditions were 30 °C, ≥ 85 % relative humidi-ty, 900 rpm shaking frequency and 200 µmol/ (m^-2 x s^-1) illumination intensity. The light spectrum was approximated to typical Mid-European daylight (Morschett et al., 2017). Illuminated cultures were run in a Photo BioLector® prototype (Morschett et al., 2017). In both setups, biomass was monitored online by scattered light. Samples for transcriptomic analysis were taken during the late growth phase . Main cultures were performed in 48 well MTP 48 B “FlowerPlates®” covered by a F GP 10 gas-permeable sealing foil (m2p labs GmbH, Baesweiler, Germany). Per well, 1000 µL medium was inoculated to an initial OD600 of 0.2. Cultivation conditions were 30 °C, ≥ 85 % relative humidi-ty, 900 rpm shaking frequency and 0 µmol/ (m^-2 x s^-1) illumination intensity. Experiment without illumination were carried out in a commercial BioLector® (m2p labs GmbH, Bae-sweiler, Germany). In both setups, biomass was monitored online by scattered light. Samples for transcriptomic analysis were taken during the late growth phase . Briefly, S. lividans TK24 cells were harvested at late growth phase. Total RNA was isolated from two biological replicates using RNeasy Mini Kit along with a DNase Kit (both from Qiagen). Initially RNA quality was checked by Trinean Xpose (Gentbrugge,Belgium) and Agilent RNA Nano 6000 kit on Agilent 2100 Bioanalyzer (Agilent Technologies, Böblingen, Germany). Samples contaminated with DNA were treated with DNase (Qiagen), cleaned as described above and rechecked by Xpose and Agilent Bioanalyzer. Ribo-Zero rRNA Removal Kit (Bacteria) from Illumina (San Diego, CA, USA) was used to remove the ribosomal RNA molecules from the isolated total RNA. Removal of rRNA was checked by Agilent RNA Pico 6000 kit on Agilent 2100 Bioanalyzer (Agilent Technologies, Böblingen, Germany).
characteristics.characteristic: description
characteristics.text: MTP BioLector (BL) cultivation standard (dark)
characteristics.characteristic: growth condition
characteristics.text: Streptomyces lividans
characteristics.characteristic: organism
characteristics.text: Streptomyces lividans
characteristics.characteristic: organism
characteristics.text: Streptomyces lividans
characteristics.characteristic: scientific_name
characteristics.text: none (dark)
characteristics.characteristic: stimulus
characteristics.text: S_lividans_TK24_Syscomp_light_Dark_strRNA-S2-DARK
characteristics.characteristic: title