characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2017-05-15T17:01:33Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-03-10T15:08:06Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA103906603
characteristics.characteristic: External Id
characteristics.text: Laboratory for Translational Genetics VIB Center for Cancer Biology (CCB) Department of Human Genetics, KUL
characteristics.characteristic: INSDC center name
characteristics.text: 2017-05-15T17:01:33Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-03-10T15:08:06Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1595771
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-5568:12_EpiLC_BS
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: B6 incipient congenic (N5; >95% B6) epiblast-like cells
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Cells have been collected from B6 incipient congenic (N5; >95% B6) blastocyst-derived embryonic stem cell line (wt) converted in vitro to epiblast-like cells. Genomic DNA was extracted using the Promega Wizard kit, treated with RNase A (NEB) and further purified using the DNeasy Blood & Tissue Kit (Qiagen). DNA was fragmented by sonication to 200-300bp and processed in parallel bisulfite conversion with and without oxidation pre-treatment using the TrueMethyl-Seq kit (Cambridge Epigenetix, UK) according to the manufacturer’s instructions. CEGX TrueMethyl-Seq kit
characteristics.characteristic: description
characteristics.text: homozygous for Tet1 gene trap GT(RRG140)
characteristics.characteristic: genotype
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: C57BL/6
characteristics.characteristic: strain
characteristics.text: 12_EpiLC_BS
characteristics.characteristic: title