characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2017-08-30T17:02:17Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-03-29T11:14:44Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA103948136
characteristics.characteristic: External Id
characteristics.text: Imperial College London
characteristics.characteristic: INSDC center name
characteristics.text: 2017-08-30T17:02:17Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-03-29T11:14:44Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1637304
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-5633:cut3day_smallRNASeq_5
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: western European house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Sciatic nerves of adult mice were cut or crushed at the sciatic notch. RNA was extracted from samples using Trizol (Invitrogen) according to manufacturer’s instructions with an additional purification step by on-column DNase treatment using the RNase-free DNase Kit (Qiagen) to ensure elimination of any genomic DNA. The integrity and quantity of RNA was determined using Qubit (Invitrogen) and Agilent 2100 Bioanalyzer (Agilent Technologies). Small RNA libraries were prepared using TruSeq Small RNA sample preparation kit (Illumina). Briefly, 3` and 5` indexed adapters were ligated to 1 g of total RNA and the product of this reaction was RT-PCR amplified. Small RNA library was size selected using Pippin Prep elution system (Sage Science). The quality of the libraries was evaluated using Agilent 2100 Bioanalyzer. All the libraries were pooled together and run on two lanes of the HiSeq 2000 platform to generate 50 bp single-end reads.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: Mus musculus domesticus
characteristics.characteristic: organism
characteristics.text: Mus musculus domesticus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10092
characteristics.characteristic: organism
characteristics.text: sciatic nerve
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0001322
characteristics.characteristic: organism part
characteristics.text: Mus musculus domesticus
characteristics.characteristic: scientific_name
characteristics.text: C57BL/6J
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000606
characteristics.characteristic: strain
characteristics.text: cut3day_smallRNASeq_5
characteristics.characteristic: title