characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2017-12-31T17:01:43Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-05-16T18:32:10Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104032774
characteristics.characteristic: External Id
characteristics.text: unspecified
characteristics.characteristic: INSDC center name
characteristics.text: 2017-12-31T17:01:43Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-05-16T18:32:10Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1696667
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-5688:AN1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: neuron
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000540
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Neurons and astrocytes were cultured as described in Bell et al., \Neuronal development is promoted by weakened intrinsic antioxidant defences due to epigenetic repression of Nrf2\, Nature Communications 6: 7066 (2015). Pure neuronal cultures or mixed astrocyte/neuronal cultures were treated ± tBHQ (10 µM) for 8h. At this point RNA was extracted and subject to RNA-seq analysis. RNA-seq libraries were prepared by Edinburgh Genomics using the Illumina TruSeq stranded mRNA-seq kit, according to the manufacturer’s protocol (Illumina).
characteristics.characteristic: description
characteristics.text: Monoculture
characteristics.characteristic: growth condition
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: AN1
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0006984
characteristics.characteristic: title