characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2017-05-21T17:01:30Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-05-16T18:32:13Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104015212
characteristics.characteristic: External Id
characteristics.text: unspecified
characteristics.characteristic: INSDC center name
characteristics.text: 2017-05-21T17:01:30Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-05-16T18:32:13Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1680050
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-5691:Sample 2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: mature reproductive cell
characteristics.characteristic: cell type
characteristics.text: Protocols: Mechanical separation of the cell types was performed at the developmental stage just before the onset of cell cleavage of reproductive cells; only this stage allows for clean separation of somatic and reproductive cells. Separation started with breaking the spheroids into parts in a Dounce homogenizer without harming the cells. Cell-size based separation of cell types was achieved by successive filtration on screens of different mesh sizes and a centrifugation step, just as previously described (Nematollahi et al., 2006). The clean separation of somatic cell sheets and reproductive cells was checked microscopically Cell-size based separation of cell types was achieved by successive filtration on screens of different mesh sizes and a centrifugation step Total RNA was extracted from separated cell types using the phenol-based TRI Reagent Total RNA isolated both from somatic and reproductive cells was prepared for RNA-seq analysis with three biological replicates each. For each cell type and biological replicate, 1 µg of total RNA was subjected to poly(A)+ RNA selection and mRNA fragmentation. This was followed by random primer cDNA synthesis and adapter ligation.
characteristics.characteristic: description
characteristics.text: before the onset of cell cleavage of reproductive cells
characteristics.characteristic: developmental stage
characteristics.text: Volvox carteri
characteristics.characteristic: organism
characteristics.text: Volvox carteri
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3067
characteristics.characteristic: organism
characteristics.text: Volvox carteri
characteristics.characteristic: scientific_name
characteristics.text: Sample 2
characteristics.characteristic: title