characteristics.text: ERC000022
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2017-06-01T17:02:08Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-03-09T21:23:00Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA101053918
characteristics.characteristic: External Id
characteristics.text: UNIVERSITY OF WESTERN ONTARIO
characteristics.characteristic: INSDC center name
characteristics.text: 2017-06-01T17:02:08Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-03-09T21:23:00Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1572853
characteristics.characteristic: SRA accession
characteristics.text: JK_RA
characteristics.characteristic: Submitter Id
characteristics.text: Illumina forward: ACA CTC TTT CCC TAC ACG ACG CTC TTC CGA TCT; illumina reverse: CGG TCT CGG CAT TCC TGC TGA ACC GCT CTT CCG ATC T
characteristics.characteristic: adapters
characteristics.text: 2014-06-14
characteristics.characteristic: collection date
characteristics.text: July Komoka invaded
characteristics.characteristic: description
characteristics.text: Temperate hardwood forest
characteristics.characteristic: environment (biome)
characteristics.text: riparian zone; sugar maple stand
characteristics.characteristic: environment (feature)
characteristics.text: soil
characteristics.characteristic: environment (material)
characteristics.text: Canada
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: 0.1
characteristics.unit: m
characteristics.characteristic: geographic location (depth)
characteristics.text: 240
characteristics.unit: m
characteristics.characteristic: geographic location (elevation)
characteristics.text: 42°57'5.87N
characteristics.unit: DD
characteristics.characteristic: geographic location (latitude)
characteristics.text: 81°23'50.43W
characteristics.unit: DD
characteristics.characteristic: geographic location (longitude)
characteristics.text: metagenome
characteristics.characteristic: investigation type
characteristics.text: AMV4.5N_7F CCAAGGCC AMDG_10R CAGGCTTA
characteristics.characteristic: multiplex identifiers
characteristics.text: https://www.researchgate.net/profile/Masanori_Saito4/publication/249467201_A_new_primer_for_discrimination_of_arbuscular_mycorrhizal_fungi_with_polymerase_chain_reaction-denature_gradient_gel_electrophoresis/links/5535e3580cf218056e92ab46.pdf?origin=publication_detail
characteristics.characteristic: nucleic acid amplification
characteristics.text: https://www.zymoresearch.com/dna/microbial-environmental-dna-isolation-1/soil-fecal-plant-dna/zr-soil-microbe-dna-microprep
characteristics.characteristic: nucleic acid extraction
characteristics.text: fungus metagenome
characteristics.characteristic: organism
characteristics.text: 94°C for 1 min, 29 cycles of 94°C for 30 s, 55°C for 30 s, 72°C for 18 s. No final extension step was used.
characteristics.characteristic: pcr conditions
characteristics.text: https://www.researchgate.net/profile/Masanori_Saito4/publication/249467201_A_new_primer_for_discrimination_of_arbuscular_mycorrhizal_fungi_with_polymerase_chain_reaction-denature_gradient_gel_electrophoresis/links/5535e3580cf218056e92ab46.pdf?origin=publication_detail
characteristics.characteristic: pcr primers
characteristics.text: PRJEB19724
characteristics.characteristic: project name
characteristics.text: 0.25
characteristics.unit: g
characteristics.characteristic: sample weight for DNA extraction
characteristics.text: fungus metagenome
characteristics.characteristic: scientific_name
characteristics.text: Illumina Miseq 2x300 bp
characteristics.characteristic: sequencing method
characteristics.text: soil
characteristics.characteristic: soil environmental package
characteristics.text: frozen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000922
characteristics.characteristic: storage conditions (fresh/frozen/other)
characteristics.text: nuclear ribosomal DNA gene, small subunit
characteristics.characteristic: target gene
characteristics.text: JK_RA
characteristics.characteristic: title