characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2019-01-31T17:02:42Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-02-24T10:30:08Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA100351918
characteristics.characteristic: External Id
characteristics.text: Leukaemia and Stem Cell Biology Group Department of Haematological Medicine King's College London
characteristics.characteristic: INSDC center name
characteristics.text: 2019-01-31T17:02:42Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-02-24T10:30:08Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1571917
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-5501:HSC.Normal.LT.CB4
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: hematopoietic stem cell
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Cells were plated in M3234 Methylcellulose medium supplemented with 20ng/ml SCF, 10ng/ml of each IL3, IL6 and GM-CSF and appropriate antibiotic selection on the following day. Colonies were scored after 7 days and replated every 7 days. 300ng to 1ug of mirVANA (Ambion) isolated total RNA was used for RNA-Seq library preparation using TruSeq Stranded Total RNA kit (Illumina) and sequenced on HIseq2000 platform (Illumina) as per manufacturers recommendations.
characteristics.characteristic: description
characteristics.text: normal
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000461
characteristics.characteristic: disease
characteristics.text: somatic genotype
characteristics.characteristic: genotype
characteristics.text: long-term culture
characteristics.characteristic: growth condition
characteristics.text: CB4
characteristics.characteristic: individual
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: umbilical cord blood
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0012168
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: HSC.Normal.LT.CB4
characteristics.characteristic: title