characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2017-04-27T17:01:59Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-05-16T18:32:08Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104031715
characteristics.characteristic: External Id
characteristics.text: unspecified
characteristics.characteristic: INSDC center name
characteristics.text: 2017-04-27T17:01:59Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-05-16T18:32:08Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1695608
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-5708:8838
characteristics.characteristic: Submitter Id
characteristics.text: 10
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Pancreatic islets (50-200) were extracted from homozygous Pax6 floxed mice which were also either hemizygous or null for Pdx1-CreERT one week after the completion of tamoxifen treatment by pancreatic distension and digestion with collagenase. Islets were incubated in RPMI media containing 11 mM glucose, 10% FCS, 100 IU/mL penicillin, 100 µg/mL streptomycin, at 5% CO2 and 37 C for 24 h. Transgenic mice were crossed to produce offspring which were all homozygous for floxed Pax6 alleles and either hemizygous or null for Pdx1-CreERT. All mice were injected with 5 daily intraperitoneal injections of tamoxifen (2 mg). RNA was extracted with TRIzol (Invitrogen) using the manufacturer's protocol, with the addition of glycogen as a co-precipitate. Resuspended RNA was treated with DNAse I (Sigma-Aldrich), which was then heat inactivated. Polyadenylated RNA was purified with oligo-dT beads and the library constructed using the TruSeq Stranded kit (Illumina). Illumina Universal Paired-end adapters were used, and custom barcoding sequences incorporated during the amplification rounds (15 cycles) for multiplexing.
characteristics.characteristic: description
characteristics.text: Pdx1-CreERT / Pax6 fl/fl
characteristics.characteristic: genotype
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: islet of Langerhans
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000006
characteristics.characteristic: organism part
characteristics.text: beta cell specific Pax6 knockout
characteristics.characteristic: phenotype
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: C57BL/6
characteristics.characteristic: strain
characteristics.text: 8838
characteristics.characteristic: title