characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2017-12-31T17:02:36Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-03-15T13:58:50Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA103913908
characteristics.characteristic: External Id
characteristics.text: OREGON STATE UNIVERS
characteristics.characteristic: INSDC center name
characteristics.text: 2017-12-31T17:02:36Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-03-15T13:58:50Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1603076
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-5583:POKKALI-RSS1-SSR
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: long-grained rice
characteristics.characteristic: common name
characteristics.text: Protocols: A leaf punch of approximately 1 centimeter in diameter was collected from plants DNA Leaf tissue from each rice variety was extracted using a Qiagen DNeasy kit per manufacturer's protocols. DNA from each rice variety was added to Promega PCR Master Mix as per manufacturer's protocol for a 50 microliter reaction. Purification of PCR product was obtained by precipitating with a 9:1 ratio of 3M sodium acetate and 1:3 ratio of 100% ethanol. After 20 minute incubation at -20 Celsius, samples were centrifuged for 30 minutes. Ethanol was removed from the sample and the pellet containing purified PCR product was dried. PCR product was resuspended in double distilled water.
characteristics.characteristic: description
characteristics.text: Oryza sativa Indica Group
characteristics.characteristic: organism
characteristics.text: Oryza sativa Indica Group
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_39946
characteristics.characteristic: organism
characteristics.text: leaf
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: organism part
characteristics.text: Oryza sativa Indica Group
characteristics.characteristic: scientific_name
characteristics.text: Pokkali GSOR: 312020
characteristics.characteristic: strain
characteristics.text: POKKALI-RSS1-SSR
characteristics.characteristic: title