characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2017-10-23T17:03:32Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-03-28T11:43:27Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA103942076
characteristics.characteristic: External Id
characteristics.text: Research Fellow Tremethick Lab Department of Genome Sciences The John Curtin School of Medical Research The Australian National University
characteristics.characteristic: INSDC center name
characteristics.text: 2017-10-23T17:03:32Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-03-28T11:43:27Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1631244
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-5628:MDCKTGFbD6_3
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: MDCK
characteristics.characteristic: cell line
characteristics.text: Epithelial cell of kidney
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0002518
characteristics.characteristic: cell type
characteristics.text: dog
characteristics.characteristic: common name
characteristics.text: Protocols: MDCK cells were maintained in DMEM growth medium supplemented with 10% Foetal Bovine Serum (FBS) For TGF-beta treatment, wildtype MDCK were stimulated in culture by adding 5ng of TGF-beta to the medium. TGF-beta was added with every passage of cells, to maintain stimulus. Total RNA was extracted usign Qiagen RNAeasy kit following manufacturer's instructions. Libraries were constructed according to the Illumina TruSeq stranded mRNA protocol with poly-A enrichment.
characteristics.characteristic: description
characteristics.text: MDCK cells were maintained in DMEM growth medium supplemented with 10% Foetal Bovine Serum (FBS)
characteristics.characteristic: growth condition
characteristics.text: Canis lupus familiaris
characteristics.characteristic: organism
characteristics.text: Canis lupus familiaris
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9615
characteristics.characteristic: organism
characteristics.text: kidney
characteristics.characteristic: organsim part
characteristics.text: Canis lupus familiaris
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: MDCKTGFbD6_3
characteristics.characteristic: title