characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2017-06-15T17:01:31Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-05-16T18:32:10Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104030614
characteristics.characteristic: External Id
characteristics.text: unspecified
characteristics.characteristic: INSDC center name
characteristics.text: 2017-06-15T17:01:31Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-05-16T18:32:10Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1694507
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-5710:AA2195DT
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: 500 ng of DNA was used. Extension-ligation was performed according to manufacturer’s protocol. Two PCR cycles were performed as follows: 95°C 3 minutes, followed by 2 cycles of 95°C for 30 seconds, 66°C for 30 seconds, and 72°C for 60 seconds. Following two cycles of PCR, Ampure Bead cleanup was carried out according to standard protocol and eluted in 25 uL. 20 uL of PCR product, 22 uL of PMM2/TDP1 and 4 uL of the i7 primer supplied with from the TruSeq Custom Amplicon Index Kit were added to PCR tubes, and PCR was carried out using the following conditions: 95°C for 30 seconds; 22 cycles of 95°C for 30 seconds, 66°C for 30 seconds, and 72°C for 60 seconds; and finally 72°C for 5 minutes
characteristics.characteristic: description
characteristics.text: breast carcinoma
characteristics.characteristic: disease
characteristics.text: AA2195
characteristics.characteristic: individual
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: breast
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000310
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: AA2195DT
characteristics.characteristic: title