characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2017-03-24T17:03:15Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-03-24T13:35:45Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA103939837
characteristics.characteristic: External Id
characteristics.text: Iowa State University
characteristics.characteristic: INSDC center name
characteristics.text: 2017-03-24T17:03:15Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-03-24T13:35:45Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1629005
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-5606:H_LPS_T0_P816
characteristics.characteristic: Submitter Id
characteristics.text: Yorkshire
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/LBO_0000338
characteristics.characteristic: breed
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: pig
characteristics.characteristic: common name
characteristics.text: Protocols: Whole blood was collected into Tempus tubes from jugular vein immediately before initial LPS injection and 2, 6 24 hours post initial injection All pigs were housed in individual metabolism pens, had free access to water and were fed a corn-soy-based diet twice daily, and feed restricted (1.5 kg/day) as previously described. After a 9-day adaptation period pigs were randomly assigned within line to either a control (n = 6, three pigs per line) or LPS challenge (n = 8, four pigs per line) group. Pigs in the challenge group were then repeatedly challenged with an intramuscular injection of 30, 36, 39, and 42 µg/kg BW of LPS from E. coli O5:B5 (sigma-Aldrich, St. Louis, MO) dissolved in saline solution at time points 0, 48, 96, and 144 hours post initial injection (hpi) to compensate for LPS tolerance after initial injection, respectively, while animals in the control group were injected with an equivalent volume of saline solution at the equivalent time points. Total RNA was extracted from the 32 blood samples of the treatment group in Replicate 2, collected at 0, 2, 6, and 24 hpi, by using preserved blood RNA purification kit I (Norgen Biotek Corp, Thorold, Ontario) per manufacturer’s instruction. On-column DNA digestion was performed as described using DNase I (Qiagen, Valencia, CA). Globin transcripts (HBB and HBA) were depleted by following an RNase H-mediated method. The quantity and integrity of the RNA were monitored by using Nanodrop 2000 (Thermo Scientific, Waltham, MA) and Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA) before and after globin depletion [176]. The efficiency of globin depletion of each sample was checked by conventional RT-qPCR with β-actin and GAPDH as the internal controls. Globin depletion efficiencies for all RNA samples were above 85%. Libraries were constructed by following Truseq library construction kit v2.0 by Beijing Genome Institue (Hong Kong)
characteristics.characteristic: description
characteristics.text: housed in individual metabolism pens, had free access to water and were fed a corn-soy-based diet twice daily, and feed restricted (1.5 kg/day)
characteristics.characteristic: growth condition
characteristics.text: 41.25
characteristics.characteristic: hematocrit
characteristics.text: 13.1
characteristics.characteristic: hemoglobin measurement
characteristics.text: 816
characteristics.characteristic: individual
characteristics.text: Sus scrofa
characteristics.characteristic: organism
characteristics.text: Sus scrofa
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9823
characteristics.characteristic: organism
characteristics.text: blood
characteristics.characteristic: organism part
characteristics.text: high residual feed intake
characteristics.characteristic: phenotype
characteristics.text: 286.5
characteristics.characteristic: platelet count
characteristics.text: Sus scrofa
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: H_LPS_T0_P816
characteristics.characteristic: title