characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2017-05-04T17:01:35Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-03-07T16:31:10Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA103893955
characteristics.characteristic: External Id
characteristics.text: The Francis Crick Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2017-05-04T17:01:35Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-03-07T16:31:10Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1583123
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-5553:GA60_C09_23
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: neural crest-derived cell
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: tdT+ cells were separated using the FACSAria Fusion (BD Biosciences) cell sorter with the 100µm nozzle at 26psi and collected in 2% FBS in OptiMEM (Life Technologies). Dead cells were excluded with a cell viability dye (Zombie Aqua, BioLegend) that was added to the single cell suspension (2 ng/ml). The Fluidigm C1 automated microfluidic system was used to randomly capture 192 individual cells into 5-10µm chips. SER93|tdT is a transgenic line Sox10-CreERT2;Rosa26-Tomato (R26-CAG-Ai14). Information regarding its generation can be found in Laranjeira et al., 2011. SER93|tdT embryos were exposed to Tamoxifen at E12.0 in utero (100µg/gr of dam body weight) and harvested 24 hours later. Individual small intestines were digested with Dispase/Collagenase (1 mg/ml, Roche, 296638) at 37°C for 15 minutes and manually triturated to obtain a single-cell suspension. The SMARTer Ultralow RNA kit (Clontech #634833) was used to reverse transcribe poly (A) RNA and amplify cDNA. ERCC spike-ins (Life Technologies #4456740) were added at a dilution of 1:80000. cDNA concentration of each cell sample was quantified using the Promega Quantifluor dsDNA on Glomax system and quality of random samples was checked on high-sensitivity DNA chip on Agilent Bioanalyser 2100. Amplified cDNA (0.125-0.375 ng) was used for sequencing libraries with the Illumina Nextera XT DNA kit (Illumina #FC-131-1096).
characteristics.characteristic: description
characteristics.text: embryonic day 12.5
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002563
characteristics.characteristic: developmental stage
characteristics.text: Sox10-CreERT2;Rosa26-tdTomato
characteristics.characteristic: genotype
characteristics.text: GA60_C09_23
characteristics.characteristic: individual
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: small intestine
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0002108
characteristics.characteristic: organism part
characteristics.text: tdTomato positive
characteristics.characteristic: phenotype
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: tamoxifen at embryonic day 12
characteristics.characteristic: stimulus
characteristics.text: mixed CBA and C57BL/10
characteristics.characteristic: strain
characteristics.text: GA60_C09_23
characteristics.characteristic: title