characteristics.text: ERC000022
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2019-01-09T17:03:21Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-03-29T17:36:53Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA103949531
characteristics.characteristic: External Id
characteristics.text: INRA-CMSE
characteristics.characteristic: INSDC center name
characteristics.text: 2019-01-09T17:03:21Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-03-29T17:36:53Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1638699
characteristics.characteristic: SRA accession
characteristics.text: MadaTR_2015_A1517_006_P2_16S
characteristics.characteristic: Submitter Id
characteristics.text: 2015-04
characteristics.characteristic: collection date
characteristics.text: This study was carried out in the agricultural region of Itasy situated in the Highlands of Madagascar and submitted to a tropical altitude climate with MAT of 18.4�C and MAP of 1319 mm. Hillsides (locally called �tanety�) of Highlands are dominated by ferrallitic soils or Ferralsols according to the FAO classification and are subjected to a hot rainy season from November to April and a cold dry season from May to October. In April 2015, i.e. at the end of the cropping season, soil samples from 49 agricultural parcels managed by smallholder famers and 4 unexploited fields were sampled in the municipality of Imerintsiatosika (18� 59_ 00_ S, 47� 19_ 00_ E). The agricultural plots were submitted to 3 land use types (subsistence crops, agroforestry and forestry) and smallholder farmers conducted different agricultural practices.In each plot, six soil cores, 0-10 cm depth, were sampled using a metallic cylinder. One core was used to measure the soil bulk density and other physicochemical parameters. The remaining 5 soil cores were pooled, sieved (2 mm), and coarse plant debris were removed. Composite samples were maintained at 4�C for one week, prior to further fresh soil incubations and analyses.
characteristics.characteristic: description
characteristics.text: terrestrial biome
characteristics.characteristic: environment (biome)
characteristics.text: anthropogenic geographic feature
characteristics.characteristic: environment (feature)
characteristics.text: soil
characteristics.characteristic: environment (material)
characteristics.text: Madagascar
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: 0,1
characteristics.unit: m
characteristics.characteristic: geographic location (depth)
characteristics.text: 1334
characteristics.unit: m
characteristics.characteristic: geographic location (elevation)
characteristics.text: -18,969383
characteristics.unit: DD
characteristics.characteristic: geographic location (latitude)
characteristics.text: 47,27795
characteristics.unit: DD
characteristics.characteristic: geographic location (longitude)
characteristics.text: metagenome
characteristics.characteristic: investigation type
characteristics.text: CATAGTAGTG
characteristics.characteristic: multiplex identifiers
characteristics.text: DNA was extracted using GnS-GII protocol as described in Terrat et al , 2012
characteristics.characteristic: nucleic acid extraction
characteristics.text: soil metagenome
characteristics.characteristic: organism
characteristics.text: F479 (5-CAGCMGCYGCNGTAANAC-3) R888 (5-CCGYCAATTCMTTTRAGT-3)
characteristics.characteristic: pcr primers
characteristics.text: CAMMiSolE_Itasy
characteristics.characteristic: project name
characteristics.text: 2
characteristics.unit: g
characteristics.characteristic: sample volume or weight for DNA extraction
characteristics.text: soil metagenome
characteristics.characteristic: scientific_name
characteristics.text: Illumina-MiSeq
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0004205
characteristics.characteristic: sequencing method
characteristics.text: soil
characteristics.characteristic: soil environmental package
characteristics.text: 16S rRNA gene
characteristics.characteristic: target gene
characteristics.text: A1517_006_P2
characteristics.characteristic: title