characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2018-03-14T17:02:58Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-03-21T09:05:09Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA103926723
characteristics.characteristic: External Id
characteristics.text: Institute of Molecular Oncology and Genomics Unit, Philipps University of Marburg
characteristics.characteristic: INSDC center name
characteristics.text: 2018-03-14T17:02:58Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-03-21T09:05:09Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1615891
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-5600:D15
characteristics.characteristic: Submitter Id
characteristics.text: 30
characteristics.unit: day
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Miz1 lox/lox mice were crossed with the desert hedgehog (Dhh)-Cre driver line (C57Bl6-Tg(Dhh- cre)1Mejr to achieve conditional ablation in Schwann cells of Miz1 exons 3 and 4, which encode the POZ domain (Sanz-Moreno et al. 2015). Mice had a mixed C57Bl6 and 129S2/SvHsd genetic background. Here, animals which were Dhh-Cre + ;Miz1 flox/flox are designated Miz∆POZ. Dissected sciatic nerve pairs of indicated ages were homogenized in TRI Reagent (Sigma, T9424). RNA was extracted, DNase treated (Macherey-Nagel, #740963), and purified using theNucleoSpin ® RNA Clean-up kit (Macherey-Nagel, #740948) according to the manufacturer’s instructions. RNA from cell culture samples was extracted, DNase treated and isolated using theNucleoSpin® RNA kit (Macherey-Nagel, #740955). Sequencing libraries were prepared with the TruSeq Stranded mRNA Kit (Illumina).
characteristics.characteristic: description
characteristics.text: Dhh-Cre / Miz1 flox/flox
characteristics.characteristic: genotype
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: sciatic nerve
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0001322
characteristics.characteristic: organism part
characteristics.text: MizPOZ expression
characteristics.characteristic: phenotype
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: mixed C57BL/6 and 129S2/SvHsd
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000601
characteristics.characteristic: strain
characteristics.text: D15
characteristics.characteristic: title