characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2018-03-18T17:02:47Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-04-06T09:11:33Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA103958186
characteristics.characteristic: External Id
characteristics.text: Sainsbury Laboratory, University of Cambridge
characteristics.characteristic: INSDC center name
characteristics.text: 2018-03-18T17:02:47Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-04-06T09:11:33Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1647354
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-5641:Sample 2
characteristics.characteristic: Submitter Id
characteristics.text: 9
characteristics.unit: day
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: thale cress
characteristics.characteristic: common name
characteristics.text: Protocols: A. thaliana seeds were surfaced sterilized and sown in petri dishes with agarified (0.8% Bacto Agar, Becton Dickinson) 0.5x MS medium (Duchefa), containing 1% sucrose and 0.05% MES at pH 5.7. After 3 days of stratification at 4°C, plants were grown in a CU-22L growth chamber (Percival) under 12/12 hours (day/night) light cycle. For the heat-induction, 7 days old plants grown at 21°C were first placed for 24 hours on top of a chilling platform build-in within the growth chamber and maintained by an external mini-chiller at 4°C, but with the chamber temperature set at 6°C. This was followed by 24 hours with the chamber set at 37°C for the stressed plants, whereas control plants were moved back to 21°C. DNeasy Plant Mini Kit (Quiagen). PCR-free libraries for genome sequencing were prepared with 1 µg of DNA using the TruSeq DNA PCR-Free Library Prep kit (Illumina); following provider’s instructions.
characteristics.characteristic: description
characteristics.text: seedling
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0007131
characteristics.characteristic: developmental stage
characteristics.text: Col-0
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005148
characteristics.characteristic: ecotype
characteristics.text: wild type
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: organism
characteristics.text: Arabidopsis thaliana
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3702
characteristics.characteristic: organism
characteristics.text: whole organism
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000468
characteristics.characteristic: organism part
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: scientific_name
characteristics.text: Sample 2
characteristics.characteristic: title