characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2020-01-03T16:00:35Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-04-11T11:25:51Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA103973224
characteristics.characteristic: External Id
characteristics.text: Laboratory of Microbial Genetics and Gene Expression, Institute of Microbiology of the CAS, v.v.i.
characteristics.characteristic: INSDC center name
characteristics.text: 2020-01-03T16:00:35Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-04-11T11:25:51Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1662392
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-5659:KOC
characteristics.characteristic: Submitter Id
characteristics.text: 3
characteristics.characteristic: biological replicate
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: Protocols: Bacillus subtilis 168 wild-type (LK1371) and RNase J1 null strain (LK1381) were grown into exponential phase (OD600 ∼0,5) at 37°C in LB medium without any antibiotic. In RNase J1 null strain, the gene encoding RNase J1, is replaced by kanamycin antibiotic resistance Cells were crosslinked with 1% formaldehyde, stopped crosslink with 125mM glycin, washed and pelleted. Cells were sonicated to obtain 200-500bp long DNA. One miligram of total protein was bound into Dynabeads – antibody complex. Antibody against RNA polymerase β (8RB13, santacruz) was used. DNA was eluated, decrosslinked and purified with Nucleotide Removal Kit (QIAGEN). Strand specific libraries were prapared with NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina (Biolabs) according to manufactuer´s instructions (Heidelberg, Germany).
characteristics.characteristic: description
characteristics.text: RNase J1 deletion
characteristics.characteristic: genotype
characteristics.text: Bacillus subtilis subsp. subtilis str. 168
characteristics.characteristic: organism
characteristics.text: Bacillus subtilis subsp. subtilis str. 168
characteristics.characteristic: organism
characteristics.text: Bacillus subtilis subsp. subtilis str. 168
characteristics.characteristic: scientific_name
characteristics.text: KOC
characteristics.characteristic: title