characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2018-07-31T17:02:12Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-05-16T18:32:05Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104062032
characteristics.characteristic: External Id
characteristics.text: unspecified
characteristics.characteristic: INSDC center name
characteristics.text: 2018-07-31T17:02:12Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-05-16T18:32:05Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1725925
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-5746:T3209-NS
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: Chemotherapy-resistant cancer, after orchiectomy
characteristics.characteristic: clinical history
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Use of tissue samples remaining after diagnosis for scientific reasons was approved by Medical Ethical Committee (MEC) of the Erasmus MC Rotterdam (The Netherlands), permission 02.981. This included the permission to use the secondary tissue without further consent. Samples were used according to the 'Code for Proper Secondary Use of Human Tissue in The Netherlands' developed by the Dutch Federation of Medical Scientific Societies (FMWV (Version 2002, update 2011)). Tissues were stored frozen in liquid nitrogen or as formalin-fixed and paraffin-embedded (FFPE) blocs. RNA was extracted from frozen tissue samples using TRIZOL, DNase treated and purified on RNeasy column (Qiagen). QC was performed on Agilent Bioanalzer using a Eukaryote total RNA Pico chip, vs 2.6. RNA samples were rRNA reduced with RiboZero Gold and library preparation was performed using the using the Ion Total RNA-seq kit
characteristics.characteristic: description
characteristics.text: testicular non-seminomatous germ cell tumor
characteristics.characteristic: disease
characteristics.text: T3209
characteristics.characteristic: individual
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: testis
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: frozen tissue sample
characteristics.characteristic: specimen with known storage state
characteristics.text: T3209-NS
characteristics.characteristic: title