characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2017-05-01T17:02:12Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-03-03T09:27:27Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA103354918
characteristics.characteristic: External Id
characteristics.text: Stanford University, School of Medicine Departments of Medicine and Genetics
characteristics.characteristic: INSDC center name
characteristics.text: 2017-05-01T17:02:12Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-03-03T09:27:27Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1575921
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-5547:HCT116_P_tcMock
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: HCT116
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002824
characteristics.characteristic: cell line
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Tumor tissue was collected separately from the right and left lesions of the mice LoVo and HCT116 cell lines were cultured under standard conditions (DMEM medium supplemented with 10% Fetal Bovine Serum). A single founding cell was cloned and expanded in vitro until it reached ~6M cells (a T75 flask). Two aliquots of ~1M cells were subcutaneously injected into opposite (right and left) flanks of a nude mouse (NSG mice for HCT117 and SCID mice for Lovo) and tumors were allowed to reach a size of ~1B cells (1cm3) before the animal was sacrificed. illumina Nextera Rapid Capture Expanded Exome Kit
characteristics.characteristic: description
characteristics.text: colorectal carcinoma
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000365
characteristics.characteristic: disease
characteristics.text: polyclonal tissue culture
characteristics.characteristic: growth condition
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: tumor cell
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002885
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: HCT116_P_tcMock
characteristics.characteristic: title