characteristics.text: ERC000013
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2017-06-24T17:02:15Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-04-25T13:21:45Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104027744
characteristics.characteristic: External Id
characteristics.text: MPIEVB
characteristics.characteristic: INSDC center name
characteristics.text: 2017-06-24T17:02:15Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-04-25T13:21:45Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1691637
characteristics.characteristic: SRA accession
characteristics.text: mainz_transfer_12
characteristics.characteristic: Submitter Id
characteristics.text: R1-TATGGTAATTGTCATCATAACGGTTCTGGCAAATATTC;R2-AGTCAGTCAGCCCTGTATCAGGCTGAAAATCTTCT
characteristics.characteristic: adapters
characteristics.text: SREI-05942
characteristics.characteristic: cage
characteristics.text: 2016
characteristics.characteristic: collection date
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: The symbiotic gut microbiota plays pivotal roles in host physiology. Despite expanding evidence of metabolic alterations contributing to the development of cardiovascular diseases, microbiota-triggered molecular pattern recognition signaling mechanisms impacting thrombosis are poorly defined. Here, we show that germ-free (GF) mice and mice deficient in the Toll-like receptor (Tlr) 2 have reduced thrombus growth following carotid artery injury relative to conventionally-raised (CONV-R) wild-type (WT) mice. Tlr2-/- mice raised under GF conditions were indistinguishable from GF WT mice, but colonization of GF WT and GF Tlr2-/- mice with a microbiota restored a significant difference in platelet deposition. We identify reduced plasma levels of von Willebrand Factor (VWF) and its reduced synthesis specifically in hepatic endothelial cells as the critical factor that determines defective Tlr2-/- platelet deposition in vivo. Platelets from Tlr2-/- mice displayed reduced integrin-mediated VWF binding and diminished adhesion to extracellular matrices in vitro, as seen with thrombosis-protected heterozygous Vwf+/- mice. Administration of VWF rescued defective platelet deposition in Tlr2-/- mice without affecting platelet deposition in WT mice. These experiments delineate an unexpected pathway in which microbiota-triggered TLR2 signaling alters the synthesis of pro-adhesive VWF by the liver endothelium and favors platelet-dependent vascular thrombosis.
characteristics.characteristic: description
characteristics.text: host-associated
characteristics.characteristic: environment (biome)
characteristics.text: cecum
characteristics.characteristic: environment (feature)
characteristics.text: cecum tissue
characteristics.characteristic: environment (material)
characteristics.text: genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: experimental factor
characteristics.text: Germany
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: 49°59'35.6N
characteristics.unit: DD
characteristics.characteristic: geographic location (latitude)
characteristics.text: 8°15'31.3E
characteristics.unit: DD
characteristics.characteristic: geographic location (longitude)
characteristics.text: gut
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0001007
characteristics.characteristic: host body habitat
characteristics.text: mucus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0002306
characteristics.characteristic: host body product
characteristics.text: cecum
characteristics.characteristic: host body site
characteristics.text: house mouse
characteristics.characteristic: host common name
characteristics.text: TLR2-/-
characteristics.characteristic: host genotype
characteristics.text: C57BL/6J
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000606
characteristics.characteristic: host infra specific name
characteristics.text: strain
characteristics.characteristic: host infra specific rank
characteristics.text: adult
characteristics.characteristic: host life stage
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: host sex
characteristics.text: 5
characteristics.characteristic: host subject id
characteristics.text: 10090
characteristics.characteristic: host taxid
characteristics.text: host-associated
characteristics.characteristic: host-associated environmental package
characteristics.text: metagenome
characteristics.characteristic: investigation type
characteristics.text: R1-GTCAAGAG,R2-ACAGCTCA
characteristics.characteristic: multiplex identifiers
characteristics.text: PMID:27053239
characteristics.characteristic: nucleic acid amplification
characteristics.text: Qiagen AllPrep DNA/RNA
characteristics.characteristic: nucleic acid extraction
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: 98�C-30s, 30 � [98�C-9s, 55�C-60s, 72�C-90s], 72�C-10 min
characteristics.characteristic: pcr conditions
characteristics.text: 27F-338R
characteristics.characteristic: pcr primers
characteristics.text: Gut microbiota regulate hepatic von Willebrand Factor synthesis and arterial thrombosis via Toll-like receptor-2
characteristics.characteristic: project name
characteristics.text: dissection, storage in RNAlater at -20°C
characteristics.characteristic: sample material processing
characteristics.text: -80
characteristics.unit: °C
characteristics.characteristic: sample storage temperature
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: Illumina MiSeq
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0004205
characteristics.characteristic: sequencing method
characteristics.text: cecum tissue
characteristics.characteristic: source material identifiers
characteristics.text: C57BL/6J
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000606
characteristics.characteristic: subspecific genetic lineage
characteristics.text: 16SrRNA
characteristics.characteristic: target gene
characteristics.text: V1V2
characteristics.characteristic: target subfragment
characteristics.text: Gut microbiota regulate hepatic von Willebrand Factor synthesis and arterial thrombosis via Toll-like receptor-2
characteristics.characteristic: title