characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2017-03-09T17:02:03Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-03-08T10:21:42Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA103894416
characteristics.characteristic: External Id
characteristics.text: Wellcome Trust Sanger Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2017-03-09T17:02:03Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-03-08T10:21:42Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1583584
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-5480:2x conc pool of cells
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: An equal mix of Control Brain RNA (3µl; FirstChoice Human Brain Total RNA; #AM7962) and ERCC spikes (3µl 1:4 dilution; #4456653) was mixed to form ‘2x Control RNA+ERCC’ master mix. We further diluted ‘2x Control RNA+ERCC’ to ‘1x Control RNA+ERCC’ with PCR grade water. We made two single cell master mix preparation using 3µl of ‘2x Control RNA+ERCC’ and ‘1x Control RNA+ERCC’ respectively instead of single cell suspension (adjusted with 34.4 µl Nuclease-Free water). The Single Cell Gel Bead kit (#120217), Single cell chip kit (#120219) and Single cell library kit (#120218) were used along with 10x GemCode Single Cell Instrument as per manufacturer specifications and manuals (Document # CG00011; Revision B).
characteristics.characteristic: description
characteristics.text: 20312
characteristics.characteristic: flow cell id
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: 2x Spike-ins and Control RNA concentration
characteristics.characteristic: protocol
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: 2x conc pool of cells
characteristics.characteristic: title