characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2017-06-08T17:01:19Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-05-16T18:32:15Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104016671
characteristics.characteristic: External Id
characteristics.text: unspecified
characteristics.characteristic: INSDC center name
characteristics.text: 2017-06-08T17:01:19Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-05-16T18:32:15Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1681509
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-5699:Mu_input_1
characteristics.characteristic: Submitter Id
characteristics.text: 32
characteristics.unit: hour
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: zebrafish
characteristics.characteristic: common name
characteristics.text: Protocols: The ChIP assay was carried out within the tgf-β mutant and sibling embryos at 32 hpf in zebrafish. The sonicated DNA was precipitated by Jun or Normal Rabbit IgG antibodies. Afterward, the precipitated DNA was collected by QIAquick PCR Purification Kit. The gene binding profiles were detected by deep-sequencing. DNA samples were end-repaired, A tailed, and adaptor ligated using TruSeq Nano DNA Sample Prep Kit (#FC-121-4002, Illumina), following the manufacturer’s instructions. 200 - 500 bp fragments were selected at the gel size fractionation step. The final size of the library was confirmed by gel electrophoresis.
characteristics.characteristic: description
characteristics.text: embryo
characteristics.characteristic: developmental stage
characteristics.text: tgfb mutant
characteristics.characteristic: genotype
characteristics.text: Danio rerio
characteristics.characteristic: organism
characteristics.text: Danio rerio
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7955
characteristics.characteristic: organism
characteristics.text: Danio rerio
characteristics.characteristic: scientific_name
characteristics.text: Mu_input_1
characteristics.characteristic: title