characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2017-12-01T17:02:44Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-03-17T09:21:47Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA103918418
characteristics.characteristic: External Id
characteristics.text: Institute of Microbiology and Infection School of Biosciences University of Birmingham
characteristics.characteristic: INSDC center name
characteristics.text: 2017-12-01T17:02:44Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-03-17T09:21:47Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1607586
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-5591:MarR-8xmyc_sample1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: Protocols: Prototypical enterotoxigenic Escherichia coli strain first isolated from a patient with cholera-like symptoms in Bangladesh prior to 1973 Cells were grown in liquid LB medium at 37 degrees with shaking Immunoprecipitations were done as described by Haycocks et al. (PLoS Pathog. 2015. 11:e1004605) using bacterial strain H10407 transformed with plasmids pAMNF or pAMNM encoding MarR N-terminally fused to 3xFLAG or 8xmyc respectively. After first growing cells to midlog phase, crosslinking, and sonication, protein DNA complexes were immunoprecipitated using anti-FLAG (Sigma). Libraries were prepared using immunoprecipited protein-DNA complexes immobilised with Protein A-sepharose. DNA fragments were then blunt ended, A-tailed and barcoded. This was done using an NEB quick blunting and ligation kit, the Klenow fragment (5’-3’ exo-, NEB) and NEXTflex ChIP-seq barcodes (Bioo Scientific). Following elution of complexes from the Protein-A sepharose, crosslinks were reversed and barcoded libraries were amplified by PCR. The number of PCR cycles was determined empirically for each library. After amplification, library concentration was quantified using Qubit analysis and real time PCR.
characteristics.characteristic: description
characteristics.text: pAMNM
characteristics.characteristic: genotype
characteristics.text: anti-FLAG
characteristics.characteristic: immunoprecipitate
characteristics.text: Escherichia coli
characteristics.characteristic: organism
characteristics.text: Escherichia coli
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_562
characteristics.characteristic: organism
characteristics.text: expression of MarR-8xmyc fusion protein
characteristics.characteristic: phenotype
characteristics.text: Escherichia coli
characteristics.characteristic: scientific_name
characteristics.text: H10407
characteristics.characteristic: strain
characteristics.text: MarR-8xmyc_sample1
characteristics.characteristic: title