characteristics.text: ERC000022
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2019-01-09T17:03:21Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-02-24T14:45:22Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA100488418
characteristics.characteristic: External Id
characteristics.text: INRA-CMSE
characteristics.characteristic: INSDC center name
characteristics.text: 2019-01-09T17:03:21Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-02-24T14:45:22Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1572099
characteristics.characteristic: SRA accession
characteristics.text: MadaTR_2014_RN7_99_18S
characteristics.characteristic: Submitter Id
characteristics.text: 2014-05
characteristics.characteristic: collection date
characteristics.text: This study was carried out in the Highlands of Madagascar, usually characterized as being above 900 m above sea level. Hillsides (locally called �tanety�) are dominated by ferrallitic soils subjected to tropical altitude climate with a hot rainy season from November to April and a cold dry season from May to October. In May 2014, soil samples from 41 plots were sampled under similar vegetation (herbaceous savannah mainly composed of the grass Aristida sp., Poaceae). Plots were located along 3 different climatic gradients (Fig. 1) allowing the discrimination of mean annual temperature and precipitation effects. Temperature and precipitation of the first climatic gradient ranged from 18.6 to 25.5�C and from 1359 and 1508mm. Along the second gradient, temperature and precipitation ranged from 17.7 to 26.8�C and from 1327 to 1817mm. Along the third gradient, temperature and precipitation ranged from 16.4 to 22�C and from 779 to 1491mm. Mean annual temperature and rainfall were extracted from the WORLDCLIM database (Hijmans et al., 2005), obtained from 1950 to 2000, on the basis of each geographic coordinates of sampling. At each plot, six soil cores, 0-10 cm depth, were sampled with a metallic cylinder. One of them was used to measure the bulk density and other physicochemical analyses. The other 5 were pooled, sieved at 2 mm, and coarse plant debris were removed. Composite samples were kept at 4�C prior to further fresh soil incubations and analyses. For ten plots along the three gradients sampling was triplicated in order to assess the soil intrinsic variability under similar climate parameters.
characteristics.characteristic: description
characteristics.text: terrestrial biome
characteristics.characteristic: environment (biome)
characteristics.text: anthropogenic geographic feature
characteristics.characteristic: environment (feature)
characteristics.text: soil
characteristics.characteristic: environment (material)
characteristics.text: Madagascar
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: 0,2
characteristics.unit: m
characteristics.characteristic: geographic location (depth)
characteristics.text: 1343
characteristics.unit: m
characteristics.characteristic: geographic location (elevation)
characteristics.text: 47,231631
characteristics.unit: DD
characteristics.characteristic: geographic location (latitude)
characteristics.text: -20,4612
characteristics.unit: DD
characteristics.characteristic: geographic location (longitude)
characteristics.text: metagenome
characteristics.characteristic: investigation type
characteristics.text: CGTATGCGAC
characteristics.characteristic: multiplex identifiers
characteristics.text: DNA was extracted using GnS-GII protocol as described in Terrat et al , 2012
characteristics.characteristic: nucleic acid extraction
characteristics.text: soil metagenome
characteristics.characteristic: organism
characteristics.text: FR1 (5-ANCCATTCAATCGGTANT-3) FF390 (5-CGATAACGAACGAGACCT-3)
characteristics.characteristic: pcr primers
characteristics.text: CAMMiSolE_Grad_Clim_Mada
characteristics.characteristic: project name
characteristics.text: 2
characteristics.unit: g
characteristics.characteristic: sample volume or weight for DNA extraction
characteristics.text: soil metagenome
characteristics.characteristic: scientific_name
characteristics.text: 454-pyrosequencing
characteristics.characteristic: sequencing method
characteristics.text: soil
characteristics.characteristic: soil environmental package
characteristics.text: 18S rRNA gene
characteristics.characteristic: target gene
characteristics.text: RN7_99
characteristics.characteristic: title