characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2020-01-03T16:00:35Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-04-11T10:40:39Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA103973000
characteristics.characteristic: External Id
characteristics.text: Laboratory of Microbial Genetics and Gene Expression, Institute of Microbiology of the CAS, v.v.i.
characteristics.characteristic: INSDC center name
characteristics.text: 2020-01-03T16:00:35Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-04-11T10:40:39Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1662168
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-5660:WT2M
characteristics.characteristic: Submitter Id
characteristics.text: 2
characteristics.characteristic: biological replicate
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: Protocols: Bacillus subtilis 168 wild-type (LK1371) and RNase J1 null strain strains (LK1381) were grown at 37°C in LB medium and harvested in exponential phase (OD600 ∼0,5). In RNase J1 null strain, the gene encoding RNase J1 is replaced by kanamycin antibiotic resistance. Two ml exponentially growing cells were treated with RNAprotect Bacteria reagent (QIAGEN), pelleted and immediately frozen. RNA was isolated by RNas Mini Kit (QIAGEN). Finally, RNA was DNase treated (TURBO DNA-free Kit, Ambion). Five micrograms of total RNA were rRNA depleted with Ribo-Zero Magnetic Kit, Gram-positive bacteria (Epicentre) and strand specific libraries prepared with Illumina compatible NEXTflex Rapid Directional RNA-Seq Kit (Bioo Scientific) according to manufactuer´s instructions
characteristics.characteristic: description
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: Bacillus subtilis subsp. subtilis str. 168
characteristics.characteristic: organism
characteristics.text: Bacillus subtilis subsp. subtilis str. 168
characteristics.characteristic: organism
characteristics.text: Bacillus subtilis subsp. subtilis str. 168
characteristics.characteristic: scientific_name
characteristics.text: WT2M
characteristics.characteristic: title