characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2018-06-14T17:03:15Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-04-05T12:45:58Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA103957809
characteristics.characteristic: External Id
characteristics.text: University of Chicago
characteristics.characteristic: INSDC center name
characteristics.text: 2018-06-14T17:03:15Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-04-05T12:45:58Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1646977
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-5596:Tbx20-KO1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: cardiac myocyte
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000746
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: The mice in this experiment were E11.5 control (Tnnt2-rtTA;TetO-Cre;Tbx20wt/loxP) and homozygous mutants (Tnnt2-rtTA;TetO-Cre;Tbx20loxp/loxP after induction with Doxycycline at E8.5. Hearts were harvested and dissociated using enzymatic digestion by collagenase (10mg/ml; Worthington) and dispase (10mg/mL, Invitrogen). Live Tnnt2-rtTA;TetO-Cre;R26-tdTom lineage traced cardiomyocyte cells were sorted on Influx Cell sorter (BD Biosciences) and collected in TRIZol reagent (Ambion) for RNA extraction. Cardiomyocyte specific Tbx20 conditional mutants were generated by breeding Tnnt2-rtTA;TetO-Cre;Tbx20+/loxp males with Tbx20loxP/loxP;R26tdTom/tdTom females. Cre expression was induced by providing Doxycycline (MP Biomedicals, cat# 198955; 1mg/mL) in drinking water to pregnant females from E8.5 onwards. Illumina library protocol
characteristics.characteristic: description
characteristics.text: embryonic day 11.5
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002562
characteristics.characteristic: developmental stage
characteristics.text: Tbx20 homozygous knockout
characteristics.characteristic: genotype
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: Black Swiss mouse strain
characteristics.characteristic: strain
characteristics.text: Tbx20-KO1
characteristics.characteristic: title