characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2018-05-01T17:05:19Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-03-30T13:56:17Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA103951474
characteristics.characteristic: External Id
characteristics.text: Research Institute of Horticulture
characteristics.characteristic: INSDC center name
characteristics.text: 2018-05-01T17:05:19Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-03-30T13:56:17Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1640642
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-5630:Sample 1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: Protocols: One-year-old, potted apple trees cultivars Idared and Free Redstar grafted on M.26 were inoculated with E. amylovora strain 650 in greenhouse conditions. Inoculation was made on actively growing shoots, punctured with a sterile needle on approximately 7 cm of their length and covered by droplets of bacterial suspension grown overnight in TY (Bacto Tryptone 0.5%, Yeast Extract 0.3%, CaCl2 0.065%) medium. After 24 h and 6 days from the inoculation time, samples were processed, and total RNA was isolated with a Total RNA Purification Kit (Norgen Biotek), as described by Kałużna et al. (2016). RNA was isolated with a Total RNA Purification Kit (Norgen Biotek), as described by Kałużna et al. (2016). mRNA was used for the library construction using NEBNext Ultra Directional RNA Library Preparation Kit for Illumina (New England Biolabs, E7420S).
characteristics.characteristic: description
characteristics.text: in vitro culture
characteristics.characteristic: growth condition
characteristics.text: Erwinia amylovora
characteristics.characteristic: organism
characteristics.text: Erwinia amylovora
characteristics.characteristic: organism
characteristics.text: Erwinia amylovora
characteristics.characteristic: scientific_name
characteristics.text: Sample 1
characteristics.characteristic: title