characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2017-08-21T17:01:37Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-02-26T20:27:51Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA100777918
characteristics.characteristic: External Id
characteristics.text: Laboratory of Pharmaceutical Microbiology Department of Pharmaceutical Sciences Ghent University
characteristics.characteristic: INSDC center name
characteristics.text: 2017-08-21T17:01:37Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-02-26T20:27:51Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1572485
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-5526:wild type stationary 1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: Protocols: Planktonic cultures were harvested by centrifugation, at cell density of 5 x 10^8 for exponential phase and 2 x 10^9 for stationary phase. Biofilms were washed, saline solution added to wells and cells detached by sonication and shaking. Cells from the saline solution were collected by centrifugation. Planktonic cultures were grown in LB, in 25 ml batches in a 250 ml flask, on a shaking incubator at 150 rpm and 37 degrees C. Biofilm cultures were grown in microtiter plates in LB. After 4 hours incubation biofilms were washed and then further incubated in fresh medium for a combined 24h. Plates were incubated statically at 37 degrees C. RNA was extracted using the RiboPure bacteria kit (Invitrogen), with the following modification to standard protocol: the proportion of ethanol added to crude extract prior to clean-up step was increased to 1.25 x to retain more small RNAs. Before DNase treatment, RNA was denatured by heating to 65°C for 5 min and DNase incubation time was increased from 30 min to 60 min. Libraries were prepared from ribo-depleted total RNA using the 'Truseq stranded Total RNA library prep' (Illumina) according to manufacturer's protocol.
characteristics.characteristic: description
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: stationary phase
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/BTO_0001899
characteristics.characteristic: growth condition
characteristics.text: Burkholderia cenocepacia
characteristics.characteristic: organism
characteristics.text: Burkholderia cenocepacia
characteristics.characteristic: organism
characteristics.text: Burkholderia cenocepacia
characteristics.characteristic: scientific_name
characteristics.text: wild type stationary 1
characteristics.characteristic: title