characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2017-06-01T17:01:26Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-05-16T18:32:16Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104028008
characteristics.characteristic: External Id
characteristics.text: unspecified
characteristics.characteristic: INSDC center name
characteristics.text: 2017-06-01T17:01:26Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-05-16T18:32:16Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1691901
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-5705:Sample 4
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: Protocols: Adult females of A. galli were collected from the small intestine of naturally infected laying hens before and on day three during a 7-day treatment period with FLBZ (Verminator®, Elanco Animal Health/Eli Lilly Danmark A/S) at 1.43 mg/kg body weight and day. Chickens were euthanized and live worms at both time points were collected from the small intestine. Three independent biological replicates were collected on both occassions. The worms were washed extensively in phosphate buffered saline (PBS) (22°C) and then immediately submerged in RNA-later (Sigma-Aldricht), until they were individually transferred into Cryo-vials (Sigma-Aldricht) and frozen at -80°C. Total RNA was extracted and purified from the anterior end of A. galli worms using the Qiagen RNeasy Kit. RNA purification was performed using RNase-Free DNase Set (Qiagen). The libraries were prepared from 1µg total RNA using the TruSeq stranded mRNA sample preparation kit (Cat RS-122-2101/2102, Illumina Inc.) including polyA selection. The library preparation was performed according to the manufactors protocol (#15031047 and #15031048, rev E.)
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: Ascaridia galli
characteristics.characteristic: organism
characteristics.text: Ascaridia galli
characteristics.characteristic: organism
characteristics.text: Ascaridia galli
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: Sample 4
characteristics.characteristic: title