characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2017-03-10T17:01:41Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-02-28T15:18:40Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA101359168
characteristics.characteristic: External Id
characteristics.text: IRCCS Mario Negri Institute For Pharmacological Research
characteristics.characteristic: INSDC center name
characteristics.text: 2017-03-10T17:01:41Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-02-28T15:18:40Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1573260
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-5502:Mouse XdhTg
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Genomic DNA from mutXDH transgenic Mouse (XdhTg). Transgenic mice were originally generated in the B6D2F1 hybrid strain (C57BL/6 X DBA2) and subsequently stabilized in C57BL/6 background by over 10 successive rounds of inter-breeding. Liver dissected from mutXDH transgenic mouse (XdhTg) DNA from liver was extracted using DNA-Mini Kit (Qiagen) and fragmented using Bioruptor (Diagenode). 380 ng DNA in 100 microL were subjected to sonication with the following parameters: power setting low, cycle 30sec on/ 90sec off, time 8 minutes in ice-cold, degassed water, resulting in average 550 bp size fragments.
characteristics.characteristic: description
characteristics.text: mutXDH transgenic mouse
characteristics.characteristic: genotype
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: liver
characteristics.characteristic: organism part
characteristics.text: glaucomatous phenotype
characteristics.characteristic: phenotype
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: C57BL/6
characteristics.characteristic: strain
characteristics.text: Mouse XdhTg
characteristics.characteristic: title