characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2017-09-25T17:03:16Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-02-28T16:31:18Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA101372668
characteristics.characteristic: External Id
characteristics.text: UOM
characteristics.characteristic: INSDC center name
characteristics.text: 2017-09-25T17:03:16Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-02-28T16:31:18Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1573278
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-5536:Meis Input PBA rep1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: The preparation of the material was as described in Kutejova et al., 2008. Second branchial arches, and posterior branchial arches connected to OFT were dissected (the whole tissue), crosslinked and frozen in order to pool enough for ChIP. Kutejova E, Engist B, Self M, Oliver G, Kirilenko P, Bobola N. Six2 functions redundantly immediately downstream of Hoxa2. Development. 2008. 135(8):1463-70. PMID: 18321982. MinElute PCR purification kit (Qiagen) ChIP-seq was performed as described in Donaldson et al., 2012, using 20ug of Meis antibody (Santa Cruz Biotechnology sc-10599X). Donaldson I, Amin S, Hensman J, Kutejova E, Rattray M, Lawrence N, Hayes A, Ward C and Bobola N. Genome-wide occupancy links Hoxa2 to Wnt-beta-catenin signaling in mouse embryonic development. Nucleic Acids Res. 2012. 40(9):3990-4001. 70 arches were used for each sample. Library preparation was performed using the TruSeq ChIP Sample Preparation kit (Illumina).
characteristics.characteristic: description
characteristics.text: embryonic day 11.5
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002562
characteristics.characteristic: developmental stage
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: posterior branchial arches connected to OFT
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: CD1
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005180
characteristics.characteristic: strain
characteristics.text: Meis Input PBA rep1
characteristics.characteristic: title