characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2017-05-31T17:02:00Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-03-06T11:43:56Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA103891171
characteristics.characteristic: External Id
characteristics.text: Center for Stem Cell Biology and Tissue Engineering, Sun Yat-sen University,
characteristics.characteristic: INSDC center name
characteristics.text: 2017-05-31T17:02:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-03-06T11:43:56Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1580339
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-5389:Sample 4
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: HeLa
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001185
characteristics.characteristic: cell line
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Cells were transfected with NC or snoRD50a ASOs before analysis total RNA were extracted by Trizol reagents (Life technology), 15 ug total RNAs were fragemented by fragment buffer (Ambion) for 10 mins in 75'c. RNAs were precipitated by ethanol overnight for library construction. Fragmented mRNA transcripts were converted into cDNA with a primer that contains an oligo(dT) sequence, two primer binding sites for nested PCR and two BamHI restriction enzyme sites. This specifically designed primer ensures this library is strand-specific. cDNAs were size-fractionated, purified, and further subject to circularization and linearization. A limited number of PCR cycles were used to amplify the linearized cDNAs.
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: Sample 4
characteristics.characteristic: title