characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2017-10-23T17:03:32Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-03-28T11:43:27Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA103942072
characteristics.characteristic: External Id
characteristics.text: Research Fellow Tremethick Lab Department of Genome Sciences The John Curtin School of Medical Research The Australian National University
characteristics.characteristic: INSDC center name
characteristics.text: 2017-10-23T17:03:32Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-03-28T11:43:27Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1631240
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-5628:MDCKshZD6_2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: MDCK
characteristics.characteristic: cell line
characteristics.text: Epithelial cell of kidney
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0002518
characteristics.characteristic: cell type
characteristics.text: dog
characteristics.characteristic: common name
characteristics.text: Protocols: MDCK cells were maintained in DMEM growth medium supplemented with 10% Foetal Bovine Serum (FBS) Cells for lentiviral transduction were grown to 70% confluency. A final concentration of 4μg/mL polybrene (SIGMA) and 10mM HEPES were combined with lentiviral-containing supernatent pre-warmed to 37 ̊C and added to cells. After incubation at 37 ̊C/5%CO2 for 24 hours, lentiviral-containing supernatant was removed and replaced with fresh complete medium. Cells were allowed to grow for another 24 hours, before harvest. Cells were washed and resuspended in 1% FBS/PBS. A 100% population of transduced cells were obtained by sterile cell sorting for GFP tagged cells. Total RNA was extracted usign Qiagen RNAeasy kit following manufacturer's instructions. Libraries were constructed according to the Illumina TruSeq stranded mRNA protocol with poly-A enrichment.
characteristics.characteristic: description
characteristics.text: MDCK cells were maintained in DMEM growth medium supplemented with 10% Foetal Bovine Serum (FBS)
characteristics.characteristic: growth condition
characteristics.text: Canis lupus familiaris
characteristics.characteristic: organism
characteristics.text: Canis lupus familiaris
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9615
characteristics.characteristic: organism
characteristics.text: kidney
characteristics.characteristic: organsim part
characteristics.text: Canis lupus familiaris
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: MDCKshZD6_2
characteristics.characteristic: title