characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2018-02-01T17:03:24Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-03-21T09:57:54Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA103926766
characteristics.characteristic: External Id
characteristics.text: Department of Biochemistry University of Cambridge UK
characteristics.characteristic: INSDC center name
characteristics.text: 2018-02-01T17:03:24Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-03-21T09:57:54Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1615934
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-5601:wt.yes.noAT.mrna.10
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: fission yeast
characteristics.characteristic: common name
characteristics.text: Protocols: Cells were grown at 32C in YE5S (Moreno et al. Methods Enzymology 194: 795). Total RNA was extracted using a hot phenol extraction method as described in Lyne et al. (2003) BMC Genomics 4:27. Clean up and DNase treatment were performed on with Purelink RNA microcolumns (Life Technologies) following the manufacturer’s instructions. Samples were ribo-depleted using Ribo-Zero Gold rRNA Removal Kit Yeast (Illumina) with a 4 ug input. 30 ng of ribo-depleted RNA was used as starting material for the NEXTflex Rapid Directional qRNA-Seq Kit (Bioo Scientific).
characteristics.characteristic: description
characteristics.text: wild type
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: YE5S medium
characteristics.characteristic: growth condition
characteristics.text: Schizosaccharomyces pombe
characteristics.characteristic: organism
characteristics.text: Schizosaccharomyces pombe
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4896
characteristics.characteristic: organism
characteristics.text: Schizosaccharomyces pombe
characteristics.characteristic: scientific_name
characteristics.text: wt.yes.noAT.mrna.10
characteristics.characteristic: title