characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2017-07-01T17:01:53Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-05-16T18:32:14Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104015254
characteristics.characteristic: External Id
characteristics.text: unspecified
characteristics.characteristic: INSDC center name
characteristics.text: 2017-07-01T17:01:53Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-05-16T18:32:14Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1680092
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-5649:B5N2Sa
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: stiff brome
characteristics.characteristic: common name
characteristics.text: Protocols: Plants were grown in controlled environments as previously described Molinari et al. (2013) Frontiers in Plant Science 4: 50 Agrobacterium transformation of brachypodium distachyon Bd21 was performed as described Vogel & Hill (2008) Plant Cell Reports 27(3): 471-478. RNA was isolated as previously described Molinari et al. (2013) Frontiers in Plant Science 4: 50. RNA-seq libraries were constructed the Ion Total RNA-Seq Kit v2 (ThermoFisher Scientific) according to the supplied protocol.
characteristics.characteristic: description
characteristics.text: early reproductive phase
characteristics.characteristic: developmental stage
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: B5 plTY73 negative
characteristics.characteristic: individual
characteristics.text: Brachypodium distachyon
characteristics.characteristic: organism
characteristics.text: Brachypodium distachyon
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_15368
characteristics.characteristic: organism
characteristics.text: stem
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0009047
characteristics.characteristic: organism part
characteristics.text: Brachypodium distachyon
characteristics.characteristic: scientific_name
characteristics.text: B5N2Sa
characteristics.characteristic: title