characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2018-08-20T17:02:52Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-03-10T14:51:49Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA103906300
characteristics.characteristic: External Id
characteristics.text: Friedrich Alexander University Erlangen-Nurnberg FAU Department Biology
characteristics.characteristic: INSDC center name
characteristics.text: 2018-08-20T17:02:52Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-03-10T14:51:49Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1595468
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-5569:H2AUb_smpl1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: Meer
characteristics.characteristic: cell line
characteristics.text: hematopoietic precursor cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0008001
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: For generation of Meer cells primary hematopoietic precursor cells were isolated from the bone marrow of Meer mice that carry a knock-in of an ENL-ER (mutant estrogen receptor ligand binding domain) portion into the mouse Mll locus. (Lit: Takacova, S. et al. Cancer Cell 21, 517-31, 2012). CD117 (c-kit) positive cells were isolated by magnetic enrichment according to the instructions of the manufacturer (CD117 enrichment kit, Miltenyi Biotech, Bergisch-Gladbach, Germany) and initially plated in methocel (MethoCult M3234, StemCellTechnologies, Koln, Germany) to purge residual stroma cells that cannot grow in semisolid media. After two rounds of growth in methylcellulose cells were expanded in RPMI1640 supplemented with 10% FCS. All media contained 50ng/ml recombinant murine (rm) SCF, 10ng/ml of rm-IL3, IL6, GM-CSF (cytokines were bought from Miltenyi Biotech, Bergisch-Gladbach, Germany) and for activation of the estrogen receptor 100nM 4-hydroxytamoxifen (TAM, Sigma, Taufkirchen, Germany). ChIP was done exactly as described in (Milne, T.A., Zhao, K. & Hess, J.L. Chromatin immunoprecipitation (ChIP) for analysis of histone modifications and chromatin-associated proteins. Methods Mol Biol 538, 409-23 (2009) with the exception that protein G-coupled paramagnetic beads from CellSignalingTechnologies (CST/NEB, Frankfurt, Germany) were used as precipitating agent. Antibodies and concentrations used : anti-H2AUb, Cell Signaling Technologies (#8240) 10µl; anti-H2BUb, Cell Signaling Technologies (#5546) 5µl ChIP Seq library construction with Illumina Kit according to the instructions of the manufacturer
characteristics.characteristic: description
characteristics.text: Mll-ENL-ER
characteristics.characteristic: genotype
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: H2AUb_smpl1
characteristics.characteristic: title