characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2017-08-02T17:01:48Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-03-14T10:54:15Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA103910698
characteristics.characteristic: External Id
characteristics.text: Oregon Health & Science University
characteristics.characteristic: INSDC center name
characteristics.text: 2017-08-02T17:01:48Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-03-14T10:54:15Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1599866
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-5556:Beta_3_b1t1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: beta cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000168
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: The islets were not cultured. High purity intact human islets were obtained from the Integrated islet distribution program (IIDP). To isolate the beta cells, islets were enzymatically dissociated into single cells and stained using surface monoclonal antibodies and sorted using FACS following published protocols (pmid:19383399 and27399229). The FACS-sorted beta cells were collected and processed for total RNA isolation. Isolation of RNA from gallbladder cells and beta cells using Qiagen RNeasy protocol TruSeq RNA sample library preparation v2 as recommended by the manufacturer Illumina
characteristics.characteristic: description
characteristics.text: wild type
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: pancreas
characteristics.characteristic: organism part
characteristics.text: Hpi1 positive
characteristics.characteristic: phenotype
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Beta_3_b1t1
characteristics.characteristic: title