characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2018-04-10T17:01:51Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-05-16T18:32:17Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104015210
characteristics.characteristic: External Id
characteristics.text: unspecified
characteristics.characteristic: INSDC center name
characteristics.text: 2018-04-10T17:01:51Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-05-16T18:32:17Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1680048
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-5687:WTmock3_S3_R1_001
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: thale cress
characteristics.characteristic: common name
characteristics.text: Protocols: Surface sterilized Arabidopsis thaliana seeds were sown on 0.5x Murashige and Skoog (MS) media (Duchefa, Netherlands) solidified with 0.8 % Agar (Duchefa, Netherlands) at pH 5.8 (adjusted with MES). After two days of stratification at 4 °C plates were transferred to growth chambers (Rumed, Rubarth Apparate GmbH) with a photoperiod of 16 h 22 °C: 8 h 18 °C, light : dark at 120-150 μmol m-2s-1 light intensity. Total RNA was extracted from approximately 75-100 mg mg frozen plant material with the SV Total RNA Isolation System (Promega Corporation, Madison, WI, USA). Quality of RNA was measured using an Agilent 2100 Bioanalyzer (Agilent Technologies). rRNA was removed with the Ribo-Zero Plant rRNA removal kit for plant leafs (Illumina). RNA libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit (New England Biolabs).
characteristics.characteristic: description
characteristics.text: 12 day old seedlings
characteristics.characteristic: developmental stage
characteristics.text: Col-0
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005148
characteristics.characteristic: ecotype
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: organism
characteristics.text: Arabidopsis thaliana
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3702
characteristics.characteristic: organism
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: scientific_name
characteristics.text: WTmock3_S3_R1_001
characteristics.characteristic: title