characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2018-03-15T17:02:19Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-03-16T11:56:38Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA103916785
characteristics.characteristic: External Id
characteristics.text: WTSI
characteristics.characteristic: INSDC center name
characteristics.text: 2018-03-15T17:02:19Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-03-16T11:56:38Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1605953
characteristics.characteristic: SRA accession
characteristics.text: R6K1
characteristics.characteristic: Submitter Id
characteristics.text: Good annotation of plasmid genomes is essential to maximise the value of the rapidly increasing volume of plasmid sequences. This short review highlights some of the current issues and suggests some ways forward. Where a well-studied related plasmid system exists we recommend that new annotation adheres to the convention already established for that system, so long as it is based on sound principles and solid experimental evidence, even if some of the new genes are more similar to homologues in different systems. Where a well-established model does not exist we provide generic gene names that reflect likely biochemical activity rather than overall purpose particularly, for example, where genes clearly belong to a type IV secretion system but it is not known whether they function in conjugative transfer or virulence. We also recommend that annotators use a whole system naming approach to avoid ending up with an illogical mixture of names from other systems based on the highest scoring match from a BLAST search. In addition, where function has not been experimentally established we recommend using just the locus tag, rather than a function-related gene name, while recording possible functions as notes rather than in a provisional name.
characteristics.characteristic: description
characteristics.text: Escherichia coli
characteristics.characteristic: organism
characteristics.text: Escherichia coli
characteristics.characteristic: scientific_name
characteristics.text: K12 J53
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002851
characteristics.characteristic: strain
characteristics.text: Annotation of Plasmid Genes
characteristics.characteristic: title