characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2017-11-17T17:02:11Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-03-20T08:41:35Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA103922794
characteristics.characteristic: External Id
characteristics.text: Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust â€“ Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK
characteristics.characteristic: INSDC center name
characteristics.text: 2017-11-17T17:02:11Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-03-20T08:41:35Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1611962
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-5530:Runx1_Pl1_F5
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: blood cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000081
characteristics.characteristic: cell type
characteristics.text: zebrafish
characteristics.characteristic: common name
characteristics.text: Protocols: A single kidney from heterozygote transgenic or wild-type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4',6-diamidino-2-phenylindole (DAPI, Beckman Coulter, cat no B30437) for mCherry/dsRed2, or propidium iodide (PI, Sigma cat no P4864) for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non-transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart-seq2 protocol 27, 28, with ERCC spike-in controls added at the same time as the oligo-dT and dNTP mixture (dilution 1:10^7, according to manufacturer's instructions)
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: 35222
characteristics.characteristic: forward scatter
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: Danio rerio
characteristics.characteristic: organism
characteristics.text: Danio rerio
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7955
characteristics.characteristic: organism
characteristics.text: kidney
characteristics.characteristic: organism part
characteristics.text: 87
characteristics.characteristic: reporter fluorescence
characteristics.text: Danio rerio
characteristics.characteristic: scientific_name
characteristics.text: 403
characteristics.characteristic: side scatter
characteristics.text: OK
characteristics.characteristic: single cell well quality
characteristics.text: Tubingen Long Fin
characteristics.characteristic: strain
characteristics.text: Runx1_Pl1_F5
characteristics.characteristic: title
characteristics.text: 1 cell
characteristics.characteristic: well information