characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2019-01-03T17:03:12Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-03-24T14:16:57Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA103939859
characteristics.characteristic: External Id
characteristics.text: Genetics and Genomic Medicine UCL GOS Institute of Child Health
characteristics.characteristic: INSDC center name
characteristics.text: 2019-01-03T17:03:12Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-03-24T14:16:57Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1629027
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-5616:JDM-RN-16-1145-MR
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: B cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000236
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: CD19+ B cells were sorted from freeze-thawed peripheral blood mononuclear cells (PBMC) by flow cytometry (FACS Aria III), using CD19 PE-Cy7 (BioLegend). DAPI was used to exclude dead cells. Sorted B cell RNA was extracted using the Arcturus PicoPure RNA Isolation Kit. Samples were processed using Illumina’s TruSeq Stranded mRNA LT sample preparation kit (p/n RS-122-2101) according to manufacturer’s instructions.
characteristics.characteristic: description
characteristics.text: juvenile dermatomyositis
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000557
characteristics.characteristic: disease
characteristics.text: 16
characteristics.characteristic: individual
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: blood
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: JDM-RN-16-1145-MR
characteristics.characteristic: title