characteristics.text: ERC000025
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2018-01-02T17:02:34Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-03-13T11:22:08Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA103910182
characteristics.characteristic: External Id
characteristics.text: FRIEDRICH SCHILLER UNIVERSITY JENA
characteristics.characteristic: INSDC center name
characteristics.text: 2018-01-02T17:02:34Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-03-13T11:22:08Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1599350
characteristics.characteristic: SRA accession
characteristics.text: No-cells control
characteristics.characteristic: Submitter Id
characteristics.text: 2015-05
characteristics.characteristic: collection date
characteristics.text: Bacterial 16S rRNA analysis was done with DNA extracted from autoclaved, deionized water. The aim was to identify microbial contaminations that are introduced during DNA extraction and/or amplicon preparation. DNA extraction was performed in parallel to the extraction of DNA from coal tar polluted groundwater and BTEX-degrading enrichment cultures. Microbial communities were analyzed by 16S rRNA amplicon sequencing of the V3/V4 region using a Illumina MiSeq 2x300 platform.
characteristics.characteristic: description
characteristics.text: freshwater biome
characteristics.characteristic: environment (biome)
characteristics.text: research facility
characteristics.characteristic: environment (feature)
characteristics.text: sterile water
characteristics.characteristic: environment (material)
characteristics.text: Germany
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: 50°55'50.9N
characteristics.unit: DD
characteristics.characteristic: geographic location (latitude)
characteristics.text: 11°35'01.1E
characteristics.unit: DD
characteristics.characteristic: geographic location (longitude)
characteristics.text: metagenome
characteristics.characteristic: investigation type
characteristics.text: miscellaneous natural or artificial environment
characteristics.characteristic: miscellaneous environmental package
characteristics.text: synthetic metagenome
characteristics.characteristic: organism
characteristics.text: Microbial communities of coal tar polluted groundwater
characteristics.characteristic: project name
characteristics.text: synthetic metagenome
characteristics.characteristic: scientific_name
characteristics.text: Illumina MiSeq 2x300 (Reagent Kit v3)
characteristics.characteristic: sequencing method
characteristics.text: 16S rRNA analysis of DNA extracted from autoclaved, deionized water which was used as negative control
characteristics.characteristic: title