characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2017-09-28T17:01:31Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-09-18T15:35:24Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104305957
characteristics.characteristic: External Id
characteristics.text: Max Delbruck Center for Molecular Medicine in the Helmholtz Association (MDC); Free University of Berlin
characteristics.characteristic: INSDC center name
characteristics.text: 2017-09-28T17:01:31Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-09-18T15:35:24Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1930935
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-6064:UN_1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: THP-1
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001253
characteristics.characteristic: cell line
characteristics.text: macrophage
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000235
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: We used the THP-1 monocytic cell line obtained from ATCC (#TIB-202). Approximately 1 million cells were used for each biological replicate. THP-1 cells were cultured in RPMI 1640 medium (Biochrom, #FG1215) supplemented with 10% fetal bovine serum (FBS, Biochrom, #S0615). All treatment compounds were added to the standard growth medium. THP-1 monocytes were differentiated to macrophages with 10 nM phorbol-12-myristate-13-acetate (PMA, Sigma, #P8139-5MG) for 48 hours. Following differentiation, cells were stimulated with 100 ng/ml LPS (Sigma-Aldrich, #L5293-2ML) for 2 hours, with 250 µM palmitate (Sigma Aldrich, #P9767) for 24 hours in the presence of 10 nM PMA or left unstimulated. RNA extraction was performed using RNeasy Mini Kit from Qiagen (#74106) using the standard procedure with 30 min on-column DNase digestion. RNA concentration and quality was controlled using NanoDrop 2000 (Thermo Fisher Scientific) and Bioanalyser 2100 (Agilent). Sequencing libraries were prepared using TruSeq Stranded mRNA Library Prep Kit (Illumina, #RS-122-2001) according to the standard procedure.
characteristics.characteristic: description
characteristics.text: acute monocytic leukemia
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000221
characteristics.characteristic: disease
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: blood
characteristics.characteristic: organism part
characteristics.text: monocyte
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000576
characteristics.characteristic: progenitor cell type
characteristics.text: 1
characteristics.characteristic: replicate
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: UN_1
characteristics.characteristic: title