characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2017-08-21T17:01:08Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-08-10T13:56:46Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104209657
characteristics.characteristic: External Id
characteristics.text: CSHL
characteristics.characteristic: INSDC center name
characteristics.text: 2017-08-21T17:01:08Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-08-10T13:56:46Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1868675
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-5957:Sample 16
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: sorghum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4558
characteristics.characteristic: common name
characteristics.text: BTX623
characteristics.characteristic: cultivar
characteristics.text: Protocols: Maize inbred line B73 and sorghum BTX623 were grown at CSHL Uplands Farm. For maize, root, shoot, leaf, and seedling were collected at 14-day-old stage; ears from stage v8; tassels from stage v7; pollen from stage r1; embryo, endosperm, and pericarp from seeds 20 days after pollination; silk from the R1 stage; and bract from the innermost husk. For sorghum tissues, root, shoot, leaf, and seedling were collected 14 days after germination; embryo, endosperm and pericarp 20 days after pollination; and pollen at the 9-10 weeks’ stage. Inflorescences were collected at the following sizes: 1-5 mm (inflorescence-1), 5-10 mm (inflorescence-2), and 1-2 cm (inflorescence-3). All tissues were immediately frozen in liquid N2. For each tissue, at least 10 plants were pooled in each of three biological replicates. Total RNA was prepared by grinding tissue in TRIzol reagent (Invitrogen 15596026) on dry ice and processing according to the protocol provided by the manufacturer. To remove DNA, an aliquot of total RNA was treated with RQ1 DNase (Promega M6101), followed by phenol/chloroform/isoamyl alcohol extraction, chloroform/isoamyl alcohol extraction using Phase Lock Gel Light tubes (5 PRIME 2302800), and ethanol precipitation. Precipitated RNA was stored at -20°C. PacBio libraries were generated as described (Wang et al. 2016). The 16-mer barcode sequences were shown in Supplementary Table S4. Tissues were barcoded, and SageELF was used for selection of five size-fractionated libraries (<1 kb, 1-2 kb, 2-3 kb, 3-5 kb, > 5kb).
characteristics.characteristic: description
characteristics.text: Sorghum bicolor
characteristics.characteristic: organism
characteristics.text: Sorghum bicolor
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4558
characteristics.characteristic: organism
characteristics.text: shoot
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0009006
characteristics.characteristic: organism part
characteristics.text: Sorghum bicolor
characteristics.characteristic: scientific_name
characteristics.text: Sample 16
characteristics.characteristic: title