characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2017-10-30T17:02:00Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-09-18T17:25:11Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104306024
characteristics.characteristic: External Id
characteristics.text: Iowa State University
characteristics.characteristic: INSDC center name
characteristics.text: 2017-10-30T17:02:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-09-18T17:25:11Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1931002
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-6038:Sample 29
characteristics.characteristic: Submitter Id
characteristics.text: 31
characteristics.unit: day
characteristics.characteristic: age
characteristics.text: Leghorn
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/LBO_0001061
characteristics.characteristic: breed
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: chicken
characteristics.characteristic: common name
characteristics.text: Protocols: To obtain tissue samples at 2, 6, and 10 days post inoculation, approximately one-third of the birds were euthanized with sodium pentobarbital solution at each of the three time points. The Harderian gland was harvested and placed into RNAlater® solution (ThermoFisher Scientific, Waltham, Massachusetts) for short-term storage. Within a week the samples were removed from RNAlater and placed into a -80°C freezer for long-term storage. RNA was isolated from the Harderian gland using the RNAqueous® kit (ThermoFisher Scientific, Waltham, Massachusetts) and samples were then DNase treated using the DNA-free™ kit (ThermoFisher Scientific, Waltham, Massachusetts). The quality of all samples was validated (RNA quality number>8.0) using the Fragment Analyzer™ (Advanced Analytical Technologies, Ankeny, Iowa). From the same RNA isolate, two cDNA libraries were made (technical replicates) A 500 ng input for each technical replicate was utilized to construct the cDNA library using TruSeq® RNA Sample Preparation v2 Guide (Illumina, San Diego, California) using the high throughput protocol. Following, cDNA library validation using the Fragment Analyzer™ (Advanced Analytical Technologies, Ankeny, Iowa).
characteristics.characteristic: description
characteristics.text: Newcastle disease
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0007395
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: HG1530_1
characteristics.characteristic: individual
characteristics.text: Gallus gallus
characteristics.characteristic: organism
characteristics.text: Gallus gallus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9031
characteristics.characteristic: organism
characteristics.text: Harderian gland
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0004187
characteristics.characteristic: organism part
characteristics.text: susceptible to Newcastle disease virus infection
characteristics.characteristic: phenotype
characteristics.text: 1
characteristics.characteristic: replicate
characteristics.text: Gallus gallus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: Sample 29
characteristics.characteristic: title