characteristics.text: https://doi.pangaea.de/10.1594/PANGAEA.879516
characteristics.characteristic: Citation
characteristics.text: ERC000030
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2018-04-26T17:19:58Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-08-14T12:45:46Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: 2017-05-31T08:24
characteristics.characteristic: Event Date/Time End
characteristics.text: SAMEA104212447
characteristics.characteristic: External Id
characteristics.text: PANGAEA Data Publisher for Earth and Environmental Science
characteristics.characteristic: INSDC center name
characteristics.text: 2018-04-26T17:19:58Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-08-14T12:45:46Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: 42.479
characteristics.unit: DD
characteristics.characteristic: Latitude End
characteristics.text: 3.17
characteristics.unit: DD
characteristics.characteristic: Longitude End
characteristics.text: ERS1871465
characteristics.characteristic: SRA accession
characteristics.text: 38
characteristics.unit: practical salinity unit
characteristics.characteristic: Salinity Sensor
characteristics.text: EMOSE_N010000693
characteristics.characteristic: Submitter Id
characteristics.text: 3
characteristics.unit: m
characteristics.characteristic: depth
characteristics.text: This sample (EMOSE_N010000693) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705310750Z_DAY2_EVENT-PUMP) occurred at position latitudeN=42.486567 and longitudeE=003.1702, on date/time=2017-05-31T07:50Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W3-20] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000701,EMOSE_N010000699,EMOSE_N010000697,EMOSE_N010000695,EMOSE_N010000691,EMOSE_N010000689,EMOSE_N010000687,EMOSE_N010000718,EMOSE_N010000830,EMOSE_N010000703,EMOSE_N010000685
characteristics.characteristic: description
characteristics.text: Westerlies Biome
characteristics.characteristic: environment (biome)
characteristics.text: [SRF] surface water layer (ENVO:00010504) within the epipelagic zone (ENVO:00000209) at the top of the photic zone (ENVO:00000209)
characteristics.characteristic: environment (feature)
characteristics.text: water (ENVO:00002006), including particulate matter (ENVO:01000060) such as planktonic material (ENVO:01000063)
characteristics.characteristic: environment (material)
characteristics.text: water
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CHEBI_15377
characteristics.characteristic: environmental package
characteristics.text: 2017-05-31T07:50
characteristics.characteristic: event date/time start
characteristics.text: http://store.pangaea.de/Projects/EMOSE2017/LOGSHEETS/EMOSE_201705310750Z_DAY2_WETLAB_size-fractionated_100L_R05-08.pdf
characteristics.characteristic: further details
characteristics.text: 2017-08-10Z
characteristics.characteristic: last update date
characteristics.text: 42.487
characteristics.unit: DD
characteristics.characteristic: latitude start
characteristics.text: 3.17
characteristics.unit: DD
characteristics.characteristic: longitude start
characteristics.text: [MS] Mediterranean Sea (MRGID:1905)
characteristics.characteristic: marine region
characteristics.text: marine metagenome
characteristics.characteristic: organism
characteristics.text: EuroMarine Open Science Experiment (EMOSE) 2017 edition: Inter-Comparison of Marine Plankton Metagenome Analysis Methods
characteristics.characteristic: project name
characteristics.text: In protocole SEQ-(100L-on-membrane)_W3-20, the sample material was pre-filtered on 20-micrometres, using a 47-mm-diameter nylon mexh filter, and concentrated on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250). A volume of 100 Litres was filtered and the 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250) was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 90 minutes, starting at 14:25 and ending at 15:55 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.
characteristics.characteristic: protocol description
characteristics.text: SEQ-(100L-on-membrane)_W3-20
characteristics.characteristic: protocol label
characteristics.text: high volume well pump
characteristics.characteristic: sample collection device
characteristics.text: This version can be used to provide data discovery services
characteristics.characteristic: sample status
characteristics.text: EMOSE2017
characteristics.characteristic: sampling campaign
characteristics.text: RV Nereis II (Observatoire Oceanologique de Banyuls)
characteristics.characteristic: sampling platform
characteristics.text: DAY2
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UO_0000033
characteristics.characteristic: sampling station
characteristics.text: marine metagenome
characteristics.characteristic: scientific_name
characteristics.text: 3
characteristics.characteristic: size fraction lower threshold
characteristics.text: 20
characteristics.characteristic: size fraction upper threshold
characteristics.text: 17
characteristics.unit: ºC
characteristics.characteristic: temperature
characteristics.text: EMOSE_201705310750Z_DAY2_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W3-20_N010000693
characteristics.characteristic: title