characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2018-06-15T17:01:58Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-08-07T10:18:50Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104202782
characteristics.characteristic: External Id
characteristics.text: EMBL - EBI & Wellcome Trust Sanger Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2018-06-15T17:01:58Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-08-07T10:18:50Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1861800
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-5989:Sample 1996
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: fibroblast derived cell line
characteristics.characteristic: cell line
characteristics.text: fibroblast
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000057
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Cells were washed with PBS and then trypsinized. Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. A mix of 3 cell lines (HPSI1013pf-wuye, HPSI1113pf-wetu, HPSI0114pf-vass) was used. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin - Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 6 hours before collection. Cells were directly processed in a 10X Chromium machine (10X Genomics) according to the manufacturer’s protocol. The Single Cell Gel Bead kit (#120217), Single cell chip kit (#120219) and Single cell library kit (#120218) were used along with 10x GemCode Single Cell Instrument as per manufacturer specifications and manuals (Document # CG00011; Revision B).
characteristics.characteristic: description
characteristics.text: normal
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000461
characteristics.characteristic: disease
characteristics.text: mix of wuye, wetu, vass
characteristics.characteristic: individual
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: dermis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0002067
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: poly I:C
characteristics.characteristic: stimulus
characteristics.text: 6
characteristics.unit: hour
characteristics.characteristic: time
characteristics.text: Sample 1996
characteristics.characteristic: title