characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2017-09-11T17:01:14Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-08-21T13:36:45Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104220736
characteristics.characteristic: External Id
characteristics.text: Rega Insitute KU Leuven
characteristics.characteristic: INSDC center name
characteristics.text: 2017-09-11T17:01:14Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-08-21T13:36:45Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1879754
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-5921:IFN-gamma_3
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: lung microvascular endothelial cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_2000016
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Murine lung endothelial cell line (L2 MVEC) was seeded in 6 well plates at the concentration of 5*10^4 cells/mL. After expanding the cells for 24 hours, they were washed with medium and stimulated for 24 hours with combinations of murine IFN-gamma (20 ng/mL) and dexamethasone (100 nM). RNA was extracted with RNeasy Mini Kit (Qiagen) according to the manufacturer's protocol. Purity of RNA was determined by NanoDrop and quality was assesed with RIN measurement (value of 10 for all samples). Per independent experiment RNA from 3 technical replicates per experimental condition was pooled and 3μg RNA/experimental condition were sequenced from a total of 3 independent experiments. Illumina TruSeq stranded mRNA kit was used and the single-end sequencing was performed. 50bp reads were sequenced.
characteristics.characteristic: description
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: lung parenchyma
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0008946
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: CBA/CaJ
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001336
characteristics.characteristic: strain
characteristics.text: IFN-gamma_3
characteristics.characteristic: title