characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2018-04-25T17:02:00Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-09-22T11:13:50Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104313389
characteristics.characteristic: External Id
characteristics.text: Cell Signal Unit, Okinawa Institute of Science and Technology Graduate University
characteristics.characteristic: INSDC center name
characteristics.text: 2018-04-25T17:02:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-09-22T11:13:50Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1938367
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-5901:Cnot1_control_liver_ActD_4h_1
characteristics.characteristic: Submitter Id
characteristics.text: 10
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Mice were maintained on a 12 hr light / dark cycle in a temperature-controlled (22ºC) barrier facility with free access to water and a normal diet (NCD, CA-1, CLEA Japan, Inc.). For comprehensive mRNA half-life profiling, we injected control (Cnot1 fl/fl) and Cnot1 LKO mice, which were placed on a tamoxifen-containing diet at 6 weeks of age for 2 weeks, intraperitoneally with 2 mg actinomycin D per g body weight for 4 and 8 hr, and then liver samples were collected. Total RNA was isolated from liver using Isogen II (Nippongene), according to the manufacturer’s protocol 1 µg of total RNA was used for RNA-seq library preparation with TruSeq Stranded mRNA LT Sample Prep Kit (Illumina), according to the manufacturer’s protocol.
characteristics.characteristic: description
characteristics.text: normal
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000461
characteristics.characteristic: disease
characteristics.text: Cnot1 flox/flox
characteristics.characteristic: genotype
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: liver
characteristics.characteristic: organism part
characteristics.text: wild type phenotype
characteristics.characteristic: phenotype
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: C57BL/6J
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000606
characteristics.characteristic: strain
characteristics.text: Cnot1_control_liver_ActD_4h_1
characteristics.characteristic: title