characteristics.text: 2011-09-30T17:01:30Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T15:27:08Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA1031589
characteristics.characteristic: External Id
characteristics.text: ED
characteristics.characteristic: INSDC center name
characteristics.text: 2011-09-30T17:01:30Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T15:27:08Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS039843
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-714:D immitis adult male
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: dog heartworm nematode
characteristics.characteristic: common name
characteristics.text: Protocols: RNA was extracted from the worms using the protocol described in Qiagen miRNeasy Mini Handbook October 2007. Several steps were carried out to convert total RNA into a library of template molecules suitable for high throughput DNA sequencing. The first step involves purifying the poly-A containing mRNA molecules using poly-T oligo-attached magnetic beads. Following purification, the mRNA is fragmented into small pieces using divalent cations under elevated temperature. Then, the cleaved RNA fragments are copied into first strand cDNA using reverse transcriptase and a high concentration of random hexamer primers. This is followed by second strand cDNA synthesis using DNA Polymerase I and RNaseH. Finally, the short cDNA fragments are prepared for sequencing on the Illumina Genome Analyzer IIx.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: Dirofilaria immitis
characteristics.characteristic: organism
characteristics.text: Dirofilaria immitis
characteristics.characteristic: organism
characteristics.text: Dirofilaria immitis
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: D immitis adult male
characteristics.characteristic: title