characteristics.text: mgm4440038.3
characteristics.characteristic: BROKER_OBJECT_ID
characteristics.text: ERC000024
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2018-10-11T17:03:08Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-09-20T18:15:15Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104312534
characteristics.characteristic: External Id
characteristics.text: San Diego State University
characteristics.characteristic: INSDC center name
characteristics.text: 2018-10-11T17:03:08Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-09-20T18:15:15Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1937512
characteristics.characteristic: SRA accession
characteristics.text: mgs15437
characteristics.characteristic: Submitter Id
characteristics.text: MGRAST
characteristics.characteristic: broker name
characteristics.text: 2005-08-05
characteristics.characteristic: collection date
characteristics.text: UTC
characteristics.characteristic: collection_timezone
characteristics.text: mgs15437 Taxonomy ID:408172
characteristics.characteristic: description
characteristics.text: 31 M
characteristics.characteristic: diss_org_carb
characteristics.text: ERS1937139
characteristics.characteristic: ebi_id
characteristics.text: marine habitat
characteristics.characteristic: environment (biome)
characteristics.text: marine habitat
characteristics.characteristic: environment (feature)
characteristics.text: marine habitat
characteristics.characteristic: environment (material)
characteristics.text: water
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CHEBI_15377
characteristics.characteristic: environmental package
characteristics.text: wgs_84
characteristics.characteristic: geodetic_system
characteristics.text: USA
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: ~10
characteristics.unit: m
characteristics.characteristic: geographic location (depth)
characteristics.text: 1.99231667
characteristics.unit: DD
characteristics.characteristic: geographic location (latitude)
characteristics.text: -157.4840667
characteristics.unit: DD
characteristics.characteristic: geographic location (longitude)
characteristics.text: Christmas (Kiritimati) Atoll
characteristics.characteristic: geographic location (region and locality)
characteristics.text: metagenome
characteristics.characteristic: investigation type
characteristics.text: 18301735
characteristics.characteristic: isol_growth_condt
characteristics.text: marine
characteristics.characteristic: metagenome_taxonomy
characteristics.text: marine metagenome
characteristics.characteristic: organism
characteristics.text: Northern Line Islands
characteristics.characteristic: project name
characteristics.text: filtering
characteristics.characteristic: samp mat process
characteristics.text: The water for the metagenomes was collected from below the boundary layer (in crevices and against the benthos) to avoid confounding problems with the water column. The sampling was conducted at the same time of day to help minimize diurnal effects. The water was collected from over ~20 m2 of reef using a modified bilge pump connected to low density polyethylene (LDPE) collapsible bags (19 l; Cole-Parmer, Vernon Hills, IL; Figure S1). 1) A large fraction containing mostly microbes, some small eukaryotes (such as dinoflagellates and protists), and a few VLPs, and 2) a small fraction containing mostly VLPs and some small microbes. To obtain these fractions the seawater was processed through a series of filters. The large eukaryotes were removed by filtering the entire sample through 100 m Nitex, into a barrel lined with a clean, high-density polyethylene bag. The filtrate was then concentrated to ~500 ml on a 100 kDa tangential flow filter (TFF), which captured the unicellular eukaryotes, microbes and VLPs (i.e., the water was removed). During the filtration, pressures were kept below 0.6 bar (10 psi) to ensure that the viruses were not destroyed. The concentrated sample was then passed through 0.45 m Sterivex filters (Millipore, Inc) using a 50 ml syringe. In this step, the large metagenomic fraction consisting of microbial cells was caught on the filter (microbiomes) and the filtrate was the small metagenomic fraction (viromes). All filtrations were performed on the research vessel, and the samples were stored for further processing in the laboratory at SDSU. The Sterivex filters were frozen at −80C. The 0.45 m filtrates (i.e., the virome) were extracted with chloroform to kill any residual cells (10% vol:vol; most viruses are resistant to chloroform) and stored at 4C.
characteristics.characteristic: samp_collect_device
characteristics.text: 150 litres
characteristics.characteristic: samp_size
characteristics.text: marine metagenome
characteristics.characteristic: scientific_name
characteristics.text: 454
characteristics.characteristic: sequencing method
characteristics.text: 4.910^61.110^6 VLPs ml−1
characteristics.characteristic: suspend_part_matter
characteristics.text: mgs15437 Taxonomy ID:408172
characteristics.characteristic: title
characteristics.text: water
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CHEBI_15377
characteristics.characteristic: water environmental package