characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2017-09-09T17:01:30Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-08-30T09:51:14Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104230588
characteristics.characteristic: External Id
characteristics.text: DarwinHealth, Inc
characteristics.characteristic: INSDC center name
characteristics.text: 2017-09-09T17:01:30Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-08-30T09:51:14Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1889606
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-6046:ShCtrlBDNF8h-1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: neuron
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000540
characteristics.characteristic: cell type
characteristics.text: Norway rat
characteristics.characteristic: common name
characteristics.text: Protocols: Hippocampi from E18 rat embryos brain were collected, treated with trypsin, and plated on poly-D-lysine (Sigma) coated 12-well plates at a density of 3 x 105 /well. Neurons were maintained in Neurobasal medium (Invitrogen) supplemented with 2% B-27 (Invitrogen) and 0.5 mM glutamine (Invitrogen). Half of the culture medium was changed every 3 days after plating. For RNA-seq, lenti-shATF4 and lenti-shCtrl viruses were used to infect cultured hippocampal neurons on 7 DIV; cells were treated with BDNF on 12 DIV; RNA samples were collected at indicated time points. Total RNA was isolated at indicated time points after BDNF treatment by using RNeasy Mini Kit (Qiagen). Reverse transcription was performed by using the First-strand cDNA Synthesis Kit (Origene) following the manufacturer’s instructions. TruSeq Stranded Total RNA LT Sample Prep Kit
characteristics.characteristic: description
characteristics.text: embryonic day 18
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002569
characteristics.characteristic: developmental stage
characteristics.text: Rattus norvegicus
characteristics.characteristic: organism
characteristics.text: Rattus norvegicus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10116
characteristics.characteristic: organism
characteristics.text: Ammon's horn
characteristics.characteristic: organism part
characteristics.text: 1
characteristics.characteristic: replicate
characteristics.text: Rattus norvegicus
characteristics.characteristic: scientific_name
characteristics.text: brain derived neurotrophic factor
characteristics.characteristic: stimulus
characteristics.text: ShCtrlBDNF8h-1
characteristics.characteristic: title