characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2018-12-30T17:01:43Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-08-08T10:22:25Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104205822
characteristics.characteristic: External Id
characteristics.text: INGM, National Institute of Molecular Genetics, “Romeo ed Enrica Invernizzi”, Milan, Italy
characteristics.characteristic: INSDC center name
characteristics.text: 2018-12-30T17:01:43Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-08-08T10:22:25Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1864840
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-5954:GMR>DeIF6 Pupa 4
characteristics.characteristic: Submitter Id
characteristics.text: 40
characteristics.unit: hour
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: fruit fly
characteristics.characteristic: common name
characteristics.text: Protocols: Fly strains were maintained on standard cornmeal food at 18°C. Flies were investigated at the third instar larva stage and in pupa 40 hours after puparium formation. RNA was extracted with mirVana isolation kit according to the manufacturer protocols (mirVana ThermoFisher #AM 1560). Libraries for Illumina sequencing were constructed from 100 ng of total RNA. Libraries for pupal retinae were prepared with random primed cDNA protocol according to GATC biotech company protocols.
characteristics.characteristic: description
characteristics.text: pupa
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0003143
characteristics.characteristic: developmental stage
characteristics.text: GMR>DeIF6
characteristics.characteristic: genotype
characteristics.text: Drosophila melanogaster
characteristics.characteristic: organism
characteristics.text: Drosophila melanogaster
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7227
characteristics.characteristic: organism
characteristics.text: retina
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000966
characteristics.characteristic: organism part
characteristics.text: DelF6 overexpression
characteristics.characteristic: phenotype
characteristics.text: Drosophila melanogaster
characteristics.characteristic: scientific_name
characteristics.text: GMR>DeIF6 Pupa 4
characteristics.characteristic: title