characteristics.text: ERC000024
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2018-04-29T17:02:57Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-08-16T16:32:14Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104216767
characteristics.characteristic: External Id
characteristics.text: UU
characteristics.characteristic: INSDC center name
characteristics.text: 2018-04-29T17:02:57Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-08-16T16:32:14Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1875785
characteristics.characteristic: SRA accession
characteristics.text: SpBlitz60
characteristics.characteristic: Submitter Id
characteristics.text: 2013-06-03
characteristics.characteristic: collection date
characteristics.text: freshwater metagenome
characteristics.characteristic: common name
characteristics.text: 39966-epilimnion
characteristics.characteristic: description
characteristics.text: 641.4526257
characteristics.unit: µmol/kg
characteristics.characteristic: dissolved oxygen
characteristics.text: aquatic
characteristics.characteristic: environment (biome)
characteristics.text: freshwater
characteristics.characteristic: environment (feature)
characteristics.text: small lake
characteristics.characteristic: environment (material)
characteristics.text: Sweden
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: 0-6
characteristics.unit: m
characteristics.characteristic: geographic location (depth)
characteristics.text: 15
characteristics.unit: m
characteristics.characteristic: geographic location (elevation)
characteristics.text: 59.8376
characteristics.unit: DD
characteristics.characteristic: geographic location (latitude)
characteristics.text: 18.6203
characteristics.unit: DD
characteristics.characteristic: geographic location (longitude)
characteristics.text: mimarks-survey
characteristics.characteristic: investigation type
characteristics.text: stratified
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0001976
characteristics.characteristic: lake stratification
characteristics.text: epilimnion
characteristics.characteristic: layer sampled
characteristics.text: TATCCTCT-TGCCTAGA
characteristics.characteristic: multiplex identifiers
characteristics.text: freshwater metagenome
characteristics.characteristic: organism
characteristics.text: initial denaturation step at 95 �C for 30 s, followed by 20 cycles of dissociation at 95 �C for 10 s, annealing at 62 �C for 30 s extension at 72 �C for 30 s, with a final extension of 2 min at 72 �C. then second round: initial denaturation step at 95 �C for 30 s, followed by 15 cycles of dissociation at 95 �C for 10 s, annealing at 66 �C for 30 s, extension at 72 �C for 30 s, with a final extension of 2 min at 72 �C
characteristics.characteristic: pcr conditions
characteristics.text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC
characteristics.characteristic: pcr primers
characteristics.text: 8.25
characteristics.characteristic: ph
characteristics.text: Lake Erken SSU rRNA gene amplicon timeseries
characteristics.characteristic: project name
characteristics.text: -80
characteristics.unit: °C
characteristics.characteristic: sample storage temperature
characteristics.text: 0.4
characteristics.unit: g
characteristics.characteristic: sample volume or weight for DNA extraction
characteristics.text: freshwater metagenome
characteristics.characteristic: scientific_name
characteristics.text: 3.16
characteristics.characteristic: secchi depth visibility (m)
characteristics.text: Illumina Miseq PE
characteristics.characteristic: sequencing method
characteristics.text: SSU rRNA
characteristics.characteristic: target gene
characteristics.text: V3-V4
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002097
characteristics.characteristic: target subfragment
characteristics.text: 16.28571429
characteristics.unit: ºC
characteristics.characteristic: temperature
characteristics.text: SpBlitz60
characteristics.characteristic: title
characteristics.text: 20
characteristics.unit: m
characteristics.characteristic: total depth of water column
characteristics.text: 646.473
characteristics.unit: µg/L
characteristics.characteristic: total nitrogen
characteristics.text: 0.49363765
characteristics.unit: µmol/L
characteristics.characteristic: total phosphorus
characteristics.text: water
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CHEBI_15377
characteristics.characteristic: water environmental package