characteristics.text: 2011-12-16T16:59:35Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T15:27:48Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA1032062
characteristics.characteristic: External Id
characteristics.text: CRUK-CRI
characteristics.characteristic: INSDC center name
characteristics.text: 2011-12-16T16:59:35Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T15:27:48Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS042605
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-740:MCF-10a_jc263
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: MCF-10a
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001200
characteristics.characteristic: cell line
characteristics.text: epithelial
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000066
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: MCF-7 cells were grown in DMEM containing 10% heat-inactivated FBS, 2 mM L glutamine, 50 U/ml penicillin and 50 _g/ml streptomycin and ZR75-1 cells were grown in RPMI containing 10% heat-inactivated FBS, 2 mM L glutamine, 50 U/ml penicillin and 50 _g/ml streptomycin. MCF10A cells were maintained in Mammary Epithelium Cell Growth Medium bullet kit (Clonetics, Lonza, MD, USA), containing mammary epithelium basal medium supplemented with bovine pituitary extract, human epidermal growth factor, hydrocortisone and GA-1000 (Gentamicin Sulfate and Amphotericin-B). To hormone deprive the cells, cells were grown in steroid-depleted medium for three days and then treated with vehicle (ethanol), 100 nM estrogen or 1 uM tamoxifen for 45 minutes or three hours. The antibodies used were anti-ER (sc-543) from Santa Cruz Biotechnologies and anti-CTCF (07-729) from Millipore. At least four 15 cm dishes of cells were used per chromatin immunoprecipitation (ChIP) and samples were processed according to standard ChIP procedures. The CTCF ChIP samples were performed as described in Carroll, 2005, Cell and the ER ChIP samples were performed as described in Schmidt, 2009, Methods. The immunoprecipitated DNA was subsequently amplified as previously described for Illumina sequencing Schmidt, 2009, Methods.
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: breast
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000310
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: MCF-10a_jc263
characteristics.characteristic: title