characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2018-06-26T17:03:41Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-08-10T11:57:26Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104208805
characteristics.characteristic: External Id
characteristics.text: Institute for Medical Immunology Universite Libre de Bruxelles
characteristics.characteristic: INSDC center name
characteristics.text: 2018-06-26T17:03:41Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-08-10T11:57:26Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1867823
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-5962:Med_2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: RAW 264.7
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001231
characteristics.characteristic: cell line
characteristics.text: macrophage
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000235
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: 10e6 RAW 264.7 cells were cultured in medium (DMEM containing 10% of FCS, L-glutamine, pyruvate, non-essential amino acids, and penicillin/streptomycin/gentamycin) in 12 well plates at 37°C with 5% of CO2 overnight. Cells were then stimulated with AS03 (1/30) for 4 or 8 hours in independent triplicates. total RNA was extracted with an RNAeasy Plus Mini Kit (QIAGEN) following manufacturer’s instructions. The RNA concentration and purity were measured with a NanoDrop spectrophotometer. RNA libraries were prepared for sequencing using Illumina Truseq Stranded mRNA library prep kit
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: blood
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: BALB/c
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000602
characteristics.characteristic: strain
characteristics.text: Med_2
characteristics.characteristic: title