characteristics.text: SAMEA1027567
characteristics.characteristic: External Id
characteristics.text: BGI
characteristics.characteristic: INSDC center name
characteristics.text: 2011-12-31T17:00:24Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T15:25:30Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS025225
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-567:3N
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Prostate tumor and adjacent normal tissues from 14 patients obtained from Shanghai Changhai Hospital were selected as the discovery cohort for RNA sequencing. RNA-seq libraries were constructed using the Illumina kit following the manufacturer's standard protocol. The poly(A) containing RNA were enriched from 5ug total RNA and fragmented to 200-250nt in size via fragmentation buffer at 70 C for 90 seconds. The first cDNA strand was synthesized with random hexamer primers using Super transcriptase II and the second-strand synthesis was performed by incubation with RNase H and DNA polymerase. End-repair was performed using Klenow polymerase, T4 DNA polymerase and T4 polynucleotide kinase. The end-repaired cDNA fragments were 3- adenylated with Klenow exo- and dATP, followed by Illumina PE adapters ligation on both ends. After recovery from agarose gel, the ligation products were amplified by 15 cycles of PCR with Phusion polymerase. The final size and yield of each library was validated on Agilent Technologies 2100 Bioanalyzer and q-PCR separately, and was diluted to 2.5pM for sequencing on a single lane of an Illumina HiSeq2000 flowcell.
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: 3N
characteristics.characteristic: title