characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2019-01-20T17:02:03Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-08-10T15:52:51Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104209706
characteristics.characteristic: External Id
characteristics.text: INGM, National Institute of Molecular Genetics, “Romeo ed Enrica Invernizzi”, Milan, Italy
characteristics.characteristic: INSDC center name
characteristics.text: 2019-01-20T17:02:03Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-08-10T15:52:51Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1868724
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-5961:RNA-seq CHX 2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: T-helper 1 cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000545
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Naive CD4+ T cells purified from the blood of healthy donors were polarized in vitro to TH1 in RPMI media with 10% FBS, 0.1% Penicillin/Streptomycin 0.1 % nonessential amino acids and 0,1% Sodium Pyruvate at 37°C and 5% CO2. For RNAseq samples, RNA was extracted using the mirVana isolation kit according to manufacturer's instructions (mirVana ThermoFisher #AM 1560) Libraries for RNAseq were prepared using Illumina TruSeq Stranded Total RNA Library Prep Kit
characteristics.characteristic: description
characteristics.text: normal
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000461
characteristics.characteristic: disease
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: blood
characteristics.characteristic: organism part
characteristics.text: RNA-seq
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002770
characteristics.characteristic: protocol
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: RNA-seq CHX 2
characteristics.characteristic: title