characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2017-08-31T17:01:57Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-08-21T17:37:23Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104221588
characteristics.characteristic: External Id
characteristics.text: Beijing Institute of Heart, Lung and Blood Vessel Diseases
characteristics.characteristic: INSDC center name
characteristics.text: 2017-08-31T17:01:57Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-08-21T17:37:23Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1880606
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-5886:Sample 2
characteristics.characteristic: Submitter Id
characteristics.text: 12
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: monocyte
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000576
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: 30 ul 10uM Cardiotoxin (CTX) was injected to TA muscle. 24 hours after injury, TA muscle was digested into single cells, and CD11b+ cells was sorted by FACS sortor. RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser. the mRNA is enriched by using the oligo(dT) magnetic beads (for eukaryotes). Mixed with the fragmentation buffer, the mRNA is fragmented into short fragments (about 200 bp). Then the first strand of cDNA is synthesized by using random hexamer-primer. Buffer, dNTPs, RNase H and DNA polymerase I are added to synthesize the second strand. The double strand cDNA is purified with magnetic beads. End reparation and 3'-end single nucleotide A (adenine) addition is then performed
characteristics.characteristic: description
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: Cardiotoxin injection into TA muscle
characteristics.characteristic: injury
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: tibialis anterior muscle
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0001385
characteristics.characteristic: organism part
characteristics.text: CD11b-positive
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0002465
characteristics.characteristic: phenotype
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: C57BL/6J
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000606
characteristics.characteristic: strain
characteristics.text: Sample 2
characteristics.characteristic: title