characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2018-03-03T17:03:05Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-08-17T10:42:17Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104216948
characteristics.characteristic: External Id
characteristics.text: Centre for Genome Enabled Biology and Medicine, University of Aberdeen
characteristics.characteristic: INSDC center name
characteristics.text: 2018-03-03T17:03:05Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-08-17T10:42:17Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1875966
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-5990:25_2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: Protocols: JC50 background strain Cells were incubated overnight in 5 ml volumes in the indicated conditions and transferred to fresh medium in 50 mL culture flasks for 4 hours (30C, 200 rpm) to an OD600 = 0.6. Half of the samples were treated with 2.5 mM DPTA NONOate. The other half of the samples remained no stress controls. RNA was obtained via Qiazol/chloroform extraction (QIAgen) according to the manufacturer’s instructions and assessed for quality by nanodrop. RNA was DNAse treated (TURBO DNAse, Ambion) according to the manufacturer’s instructions and assessed for quality by Agilent Bioanalyser (RIN>8). The sequencing library was prepared with the Ion Total RNA-Seq Kit v2 from Thermo Fisher Scientific (Life Tech) using ribosomal RNA depleted total RNA.
characteristics.characteristic: description
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: YPD
characteristics.characteristic: growth condition
characteristics.text: Candida albicans
characteristics.characteristic: organism
characteristics.text: Candida albicans
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_5476
characteristics.characteristic: organism
characteristics.text: Sequencing Run 2
characteristics.characteristic: replicate
characteristics.text: Candida albicans
characteristics.characteristic: scientific_name
characteristics.text: JC50
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0004556
characteristics.characteristic: strain
characteristics.text: 25_2
characteristics.characteristic: title