characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2017-09-28T17:01:30Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-09-18T15:27:26Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104305864
characteristics.characteristic: External Id
characteristics.text: Max Delbruck Center for Molecular Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2017-09-28T17:01:30Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-09-18T15:27:26Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1930842
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-6075:UN_C10
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: THP-1
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001253
characteristics.characteristic: cell line
characteristics.text: macrophage
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000235
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: We used the THP-1 monocytic cell line obtained from ATCC (#TIB-202). After differentiation and stimulation, cells were harvested, loaded into Fluidigm C1 integrated fluidic circuits (IFCs) , and cell capturing in microcells was performed according to the standard recommended procedure from Fluidigm. After cell capturing, IFC wells with cells were imaged using light microscopy. THP-1 cells were cultured in RPMI 1640 medium (Biochrom, #FG1215) supplemented with 10% fetal bovine serum (FBS, Biochrom, #S0615). All treatment compounds were added to the standard growth medium. THP-1 monocytes were differentiated to macrophages with 10 nM phorbol-12-myristate-13-acetate (PMA, Sigma, #P8139-5MG) for 48 hours. Following differentiation, cells were stimulated with 100 ng/ml LPS (Sigma-Aldrich, #L5293-2ML) for 2 hours, with 250 µM palmitate (Sigma Aldrich, #P9767) for 24 hours in the presence of 10 nM PMA or left unstimulated. Reverse transcription and pre-amplification was performed without RNA extraction directly after cell lysis in C1 IFC according to the standard protocol using SMARTer Ultra Low Input kit (Clontech, #634936). Sequencing libraries were prepared using Nextera XT library preparation kit (Illumina, #FC-131-1096/#FC-131-1002) according to the standard procedure recommended by Fluidigm; 96-cell library pools, barcoded with Nextera indexes, were prepared for each treatment condition.
characteristics.characteristic: description
characteristics.text: acute monocytic leukemia
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000221
characteristics.characteristic: disease
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: blood
characteristics.characteristic: organism part
characteristics.text: OK
characteristics.characteristic: pre-analysis well quality
characteristics.text: monocyte
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000576
characteristics.characteristic: progenitor cell type
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: UN_C10
characteristics.characteristic: title