characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2018-04-05T17:02:53Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-08-14T16:51:25Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104212604
characteristics.characteristic: External Id
characteristics.text: CSHL
characteristics.characteristic: INSDC center name
characteristics.text: 2018-04-05T17:02:53Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-08-14T16:51:25Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1871622
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-5956:group 8
characteristics.characteristic: Submitter Id
characteristics.text: not available
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001071
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: sorghum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4558
characteristics.characteristic: common name
characteristics.text: BTX623
characteristics.characteristic: cultivar
characteristics.text: Protocols: Sorghum BTX623 were grown at CSHL Uplands Farm. Root, shoot, leaf, and seedling were collected 14 days after germination; embryo, endosperm and pericarp 20 days after pollination; and pollen at the 9-10 weeks’ stage. Inflorescences were collected at the following sizes: 1-5 mm (inflorescence-1), 5-10 mm (inflorescence-2), and 1-2 cm (inflorescence-3). All tissues were immediately frozen in liquid N2. For each tissue, at least 10 plants were pooled in each of three biological replicates. Total RNA was prepared by grinding tissue in TRIzol reagent (Invitrogen 15596026) on dry ice and processing according to the protocol provided by the manufacturer. To remove DNA, an aliquot of total RNA was treated with RQ1 DNase (Promega M6101), followed by phenol/chloroform/isoamyl alcohol extraction, chloroform/isoamyl alcohol extraction using Phase Lock Gel Light tubes (5 PRIME 2302800), and ethanol precipitation. Precipitated RNA was stored at -20°C。 Total RNA (20 μg) was used for poly(A)+ selection using oligo(dT) magnetic beads (Invitrogen 610-02), eluted in water, and subjected to RNA-seq library construction using the ScriptSeq™ kit (Epicentre SS10906).
characteristics.characteristic: description
characteristics.text: 20 days after pollination
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0009010
characteristics.characteristic: developmental stage
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: Sorghum bicolor
characteristics.characteristic: organism
characteristics.text: Sorghum bicolor
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4558
characteristics.characteristic: organism
characteristics.text: endosperm
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0009089
characteristics.characteristic: organism part
characteristics.text: Sorghum bicolor
characteristics.characteristic: scientific_name
characteristics.text: group 8
characteristics.characteristic: title