characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2017-09-24T17:01:55Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-08-10T17:44:06Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104209879
characteristics.characteristic: External Id
characteristics.text: Department of Computer Science and Applied Mathematics, Weizmann Institute of Science
characteristics.characteristic: INSDC center name
characteristics.text: 2017-09-24T17:01:55Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-08-10T17:44:06Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1868897
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-5968:729
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: HeLa
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001185
characteristics.characteristic: cell line
characteristics.text: epithelial cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000066
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: No collection. Commercial Hela genomic DNA (NEB) The following scWGA kits were used (Kit short in manuscript, Kit name, Cat. Number, WGA technique, Manufacturer): Ampli1, Ampli1 WGA Kit, WG 001 050 R02, Linker adapter PCR (LA-PCR), Silicon Biosystems MALBAC, MALBAC Single Cell WGA Kit, YK001B, Multiple Annealing and Looping Based Amplification Cycles (MALBAC), Yikon Genomics GenomePlex, GenomePlex Single Cell Whole Genome Amplification Kit (WGA4), WGA4, Degenerate oligonucleotide-primed PCR (DOP-PCR), Sigma PicoPlex, PicoPLEX WGA Kit, E2620S, displacement DOP-PCR (D-DOP-PCR), New England Biolabs (manufactured by Rubicon) GPHI-SC, illustra Single Cell GenomiPhi DNA Amplification Kit, 29-1081-07, Multiple Displacement amplification (MDA), GE Healthcare RepliG-SC, REPLI-g Single Cell Kit, 150345, MDA, Qiagen TruePrime, TruePrime Single Cell WGA kit, 350025, MDA, SYGNIS Targeted enrichment using Access Array (Fluidigm) using the protocol as described in (Biezuner et al. Genome Research. 2016.) Sequencing primers are described in manuscript Supplementary Table 1.
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: GenomePlex
characteristics.characteristic: scWGA kit
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: 729
characteristics.characteristic: title