characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2017-10-31T17:01:52Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-09-01T11:03:36Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104233507
characteristics.characteristic: External Id
characteristics.text: Laboratory of Molecular Cytogenetics, Institute of Experimental Biology, Faculty of Science, Masaryk University Brno, Czech republic
characteristics.characteristic: INSDC center name
characteristics.text: 2017-10-31T17:01:52Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-09-01T11:03:36Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1892525
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-6036:Sample 1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: plasma cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000786
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Female patient was diagnosed with MM in the Department of Internal Medicine - Hematooncology (IHOK), University Hospital Brno, in 11/2007. Patient’s biological material was obtained after she signed the informed consent form approved by the Ethical committee of the hospital Plasma cells (CD138+) a were obtained from the bone marrow of multiple myeloma patients during routine clinical procedure. Plasma cells (CD138+) in mononuclear cell fraction were enriched by anti-CD138 immunomagnetic beads and sorted using magnetic activated cell sorting, MACS (AutoMACS, Miltenyi Biotec). Purity of CD138+ fraction was measured by flowcytometry. Genomic DNA was extracted from sorted cells sing the Puregene Core kit according to manufacturer´s protocol. Extracted gDNA was diluted in TE buffer and stored in -20C. The capture probes are based on ultra-long RNA baits (120 nucleotides) with high sensitivity and specificity for the detection of SNPs and indels. Probe design captures all the coding regions of 42 genes including splicing sequences plus exonic hotspots of an additional 20 genes.
characteristics.characteristic: description
characteristics.text: multiple myeloma
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001378
characteristics.characteristic: disease
characteristics.text: NRAS mutation c.181C>A; p.Gln61Lys
characteristics.characteristic: genotype
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: bone marrow
characteristics.characteristic: organism part
characteristics.text: CD138 positive
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000786
characteristics.characteristic: phenotype
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: Sample 1
characteristics.characteristic: title