characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2018-03-01T17:01:50Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-08-30T17:51:22Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104231786
characteristics.characteristic: External Id
characteristics.text: Department of Plant Protection Biology, Swedish University of Agricultural Sciences
characteristics.characteristic: INSDC center name
characteristics.text: 2018-03-01T17:01:50Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-08-30T17:51:22Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1890804
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-5996:L2
characteristics.characteristic: Submitter Id
characteristics.text: 5
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: potato
characteristics.characteristic: common name
characteristics.text: Desiree x SW93-1015
characteristics.characteristic: cultivar
characteristics.text: Protocols: Potato plants were grown under controlled conditions with 16 h/8 h day and night regime with a fluorescent lamp light intensity of 200 μmol m-2 s-1. The temperature was set to 20°C and the relative humidity was 65%. Pots were circulated every week to avoid positional effects. All sampling was done before flowering and only fully expanded leaves from the middle part of the plants were used. RNA was isolated by RNeasy Plant Mini Kit (Qiagen) Beads with oligo(dT) were used to isolate poly(A) mRNA. Fragmentation buffer was added for interrupting mRNA to short fragments. Taking these short fragments as templates, Random hexamerprimer were used to synthesize the first-strand cDNA. The second-strand cDNA was synthesized using buffer, dNTPs, RNase H and DNA polymerase I, respectively. Short fragments were purified with QiaQuick PCR extraction kit and resolved with EB buffer for end reparation and adding poly(A). After that, the short fragments were connected with sequencing adaptors. And, for amplification with PCR, we selected suitable fragments, as templates, with respect to the result of agarose gel electrophoresis.
characteristics.characteristic: description
characteristics.text: L2
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002719
characteristics.characteristic: individual
characteristics.text: Solanum tuberosum
characteristics.characteristic: organism
characteristics.text: Solanum tuberosum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4113
characteristics.characteristic: organism
characteristics.text: leaf
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: organism part
characteristics.text: Solanum tuberosum
characteristics.characteristic: scientific_name
characteristics.text: L2
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002719
characteristics.characteristic: title