characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2018-01-14T17:01:58Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-09-12T18:52:29Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104293467
characteristics.characteristic: External Id
characteristics.text: 1-Division of Pediatric General and Thoracic Surgery, Cincinnati Children’s Hospital Medical Center 2-
characteristics.characteristic: INSDC center name
characteristics.text: 2018-01-14T17:01:58Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-09-12T18:52:29Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1918445
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-6017:Infant_SI_14078
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Total tissue was collected in RLT Plus buffer (Quiagen) supplemented with 0.1% 2-Mercaptoethanol in FastPrep lysing Matrix D tubes. Prior RNA extraction, total tissue collected in RLT Plus buffer were lysed using a FastPrep machine. Total RNA was extracted using an RNeasy Plus Micro Kit (Qiagen) according to manufacturer guidelines. The libraries were constructed according to manufacturer guidelines. (Illumina). Briefly, 350 to 900 ng of total RNA determined by Qbit high sensitivity spectrofluorometric measurement (Invitrogen) was poly-A selected and reverse transcribed using Illumina’s TruSeq RNA library preparation kit V2. Each sample was fitted with a unique adapter containing a 6 base molecular barcode for high level multiplexing. Prior sequencing, a 12 cycles of PCR amplification was performed on the samples.
characteristics.characteristic: description
characteristics.text: infant
characteristics.characteristic: developmental stage
characteristics.text: normal
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000461
characteristics.characteristic: disease
characteristics.text: native tissue
characteristics.characteristic: growth condition
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: jejunum
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Infant_SI_14078
characteristics.characteristic: title