characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2018-12-30T17:01:43Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-08-08T10:22:25Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104205809
characteristics.characteristic: External Id
characteristics.text: INGM, National Institute of Molecular Genetics, “Romeo ed Enrica Invernizzi”, Milan, Italy
characteristics.characteristic: INSDC center name
characteristics.text: 2018-12-30T17:01:43Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-08-08T10:22:25Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1864827
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-5954:GMR/+ Larva 1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: fruit fly
characteristics.characteristic: common name
characteristics.text: Protocols: Fly strains were maintained on standard cornmeal food at 18°C. Flies were investigated at the third instar larva stage and in pupa 40 hours after puparium formation. RNA was extracted with mirVana isolation kit according to the manufacturer protocols (mirVana ThermoFisher #AM 1560). Libraries for Illumina sequencing were constructed from 100 ng of total RNA. Libraries for eye imaginal disc were prepared with the Illumina TruSeq RNA Sample Preparation Kit.
characteristics.characteristic: description
characteristics.text: third instar larva stage
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002682
characteristics.characteristic: developmental stage
characteristics.text: GMR/+
characteristics.characteristic: genotype
characteristics.text: Drosophila melanogaster
characteristics.characteristic: organism
characteristics.text: Drosophila melanogaster
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7227
characteristics.characteristic: organism
characteristics.text: eye disc
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/FBbt_00001768
characteristics.characteristic: organism part
characteristics.text: wild type phenotype
characteristics.characteristic: phenotype
characteristics.text: Drosophila melanogaster
characteristics.characteristic: scientific_name
characteristics.text: GMR/+ Larva 1
characteristics.characteristic: title