characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2017-09-22T17:03:01Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-09-19T16:12:12Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104310475
characteristics.characteristic: External Id
characteristics.text: Boehringer Ingelheim Pharma GmbH & Co. KG Target Discovery Research
characteristics.characteristic: INSDC center name
characteristics.text: 2017-09-22T17:03:01Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-09-19T16:12:12Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1935453
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-6081:Sample 68
characteristics.characteristic: Submitter Id
characteristics.text: 7 to 8
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: Rn103
characteristics.characteristic: animal id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: Norway rat
characteristics.characteristic: common name
characteristics.text: Protocols: Male Wistar Han rats (Crl:WI(Han)) and male BL/6J mice (C57BL/6J) were obtained from Charles River Laboratories (Germany). Experimental protocols concerning the use of laboratory animals were reviewed by a German Federal Ethics Committee and approved by German governmental authorities. Animals were housed in groups of three on a 12-h light/dark cycle and fed ad libitum a standard pelleted rodent diet (Diet No. 3438, Provimi Kliba Switzerland) with free access to water. Rats with a body weight of 160-180 g and rats and mice at the age of 7-8 weeks were used for tissue sampling. Retrobulbar blood samples were collected in the morning at 9 a.m. under isofluran anesthesia immediately prior to dissection. Animals (n = 3 for each species) were sacrificed thereafter by intraperitoneal injection of pentobarbital (rats) or cervical dislocation (mice) and tissues (esophagus, stomach, duodenum, jejunum, ileum, colon, pancreas, liver, thymus, kidney, heart, brain, quadriceps muscle) were harvested and transferred immediately to RNA Later at 4 degrees celsius. Total RNAs were individually extracted using the Ambion Magmax™-96 total RNA isolation kit (Life Sciences) according to the manufacturer’s instructions. Briefly, 5 mg of tissue was placed in the lysis solution and homogenized in Qiagen Tissuelyzer™ for a period of 30 sec. Nucleic acids were captured onto magnetic beads, washed and treated with DNase. Total RNA was then eluted in 50 μl elution buffer. RNA quality and concentration was measured using an RNA Pico chip on an Agilent Bioanalyzer. The Sequencing library preparation has been done using 200 ng of total RNA input with the TrueSeq RNA Sample Prep Kit v2-Set B (RS-122-2002, Illumina Inc, San Diego, CA) producing a 275bp fragment including adapters in average size. In the final step before sequencing, eight individual libraries were normalized and pooled together using the adapter indices supplied by the manufacturer.
characteristics.characteristic: description
characteristics.text: normal
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000461
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: Rattus norvegicus
characteristics.characteristic: organism
characteristics.text: Rattus norvegicus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10116
characteristics.characteristic: organism
characteristics.text: duodenum
characteristics.characteristic: organism part
characteristics.text: 199_69
characteristics.characteristic: sample id
characteristics.text: Rattus norvegicus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: Wistar Han
characteristics.characteristic: strain
characteristics.text: Sample 68
characteristics.characteristic: title