characteristics.text: 2011-11-01T17:00:25Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T15:25:19Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA1022694
characteristics.characteristic: External Id
characteristics.text: CRUK-CRI
characteristics.characteristic: INSDC center name
characteristics.text: 2011-11-01T17:00:25Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T15:25:19Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS017364
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-437:TC1 input 2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: hepatocytes
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000182
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: The protocol describing the mouse strain TC1 can be found at http://dx.doi.org/10.1126/science.1114535. For the TC1 analysis the obtained reads were aligned to the entire mouse genome + an additional human chromsome 21 and regions of known deletions and insertions in this chromosome were removed. Hepatocytes were prepared by direct perfusion of the liver with buffered salt solution followed by percent formaldehyde. After 10 minutes the tissue was removed and diced in 500 mM glycine buffer to neutralize the formaldehyde. After homogenization the hepatocytes were rinsed with PBS.
characteristics.characteristic: description
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: liver
characteristics.characteristic: tissue
characteristics.text: TC1 input 2
characteristics.characteristic: title