characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2018-12-30T17:01:43Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-08-22T11:59:51Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104222292
characteristics.characteristic: External Id
characteristics.text: Department of Bioinformatics,Tongji University
characteristics.characteristic: INSDC center name
characteristics.text: 2018-12-30T17:01:43Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-08-22T11:59:51Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1881310
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-5985:Pwp1 (-/+) OE Pwp1 input
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: E14.1
characteristics.characteristic: cell line
characteristics.text: embryonic stem cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0002322
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: mES cells (E14.1) were cultured on plates coated with 0.1% gelatin (Millipore) in high-glucose Dulbecco’s modiﬁed Eagle’s medium (DMEM; HyClone) supplemented with 15% ES cell-qualiﬁed fetal bovine serum (Gibco), 1% penicillin and streptomycin (Gibco), 2 mM L-glutamine (Gibco), 0.1 mM non-essential amino acids (NEAAs; Gibco), 0.1 mM 2-mercaptoethanol (Gibco) and 1000 units/ml of LIF (Chemicon). Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody. Libraries were prepared for sequencing using standard Illumina protocols at BGI-Shenzhen (sequencing service provider).
characteristics.characteristic: description
characteristics.text: embryo
characteristics.characteristic: developmental stage
characteristics.text: Pwp1 -/+ and Pwp1 overexpression
characteristics.characteristic: genotype
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: Pwp1 (-/+) OE Pwp1 input
characteristics.characteristic: title