characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2017-10-02T17:03:33Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-09-22T15:45:39Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104313571
characteristics.characteristic: External Id
characteristics.text: University of Muenster, Medical Faculty, Institute of Bioinformatics
characteristics.characteristic: INSDC center name
characteristics.text: 2017-10-02T17:03:33Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-09-22T15:45:39Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1938549
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-6111:genome
characteristics.characteristic: Submitter Id
characteristics.text: 19
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: CADO ES1
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002818
characteristics.characteristic: cell line
characteristics.text: Ewings sarcoma
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Ewing Sarcoma cell lines CADO-ES1, was purchased form DSMZ. Ewing sarcoma cell lines were cultivated in RPMI1640 medium, supplemented with 10% (v/v) FCS, 1% Glutamin and 1% of an antibiotic cocktail containing penicillin and streptomycin. All Ewing sarcoma cell lines were maintained on cell culture plastic coated with collagen (0,5mg/ml). Culture media was supplemented by 1% of an antibiotic cocktail containing penicillin and streptomycin and incubated at 37°C and 5%CO2 in a humidified atmosphere. Routine microbiological analysis was done using a PCR Mycoplasma Detection Kit according to suggested protocols (Applied Biosystems, Darmstadt, Germany). The extraction of nucleic acids from cell cultures was performed by the use of the DNeasy Kit (Qiagen) according to the supplier's recommendations. For DNA library preparation 500 - 5000ng DNA were used. ERCCs as an additional internal quality control were added to the libraries. We followed exactly the Thermo-Fischer/Life-Technology(ABI) protocols for the SOLID-5500XL Technology for DNAseq. Most of the procedures were run in a core lab facility of Life-Technology as a commercial service.
characteristics.characteristic: description
characteristics.text: Ewing sarcoma
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000174
characteristics.characteristic: disease
characteristics.text: EWS-ERG
characteristics.characteristic: genotype
characteristics.text: pleural fluid
characteristics.characteristic: metastatic site
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: bone
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: genome
characteristics.characteristic: title