characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2018-02-28T17:01:47Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-09-20T15:48:49Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104312503
characteristics.characteristic: External Id
characteristics.text: Mississippi State University
characteristics.characteristic: INSDC center name
characteristics.text: 2018-02-28T17:01:47Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-09-20T15:48:49Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1937481
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-6070:speE_1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: Protocols: Bacterial strains were cultured in vitro in Todd-Hewitt Yeast extract medium. Bacteria were harvested from mid-log phase cultures and total RNA was isolated using RNEasy kit (Qiagen) and quality assessed by Bioanalyzer. Illumina’s Ribozero Gold Epidemiology kit was used for Ribosomal RNA depletion. Library was prepared using KAPA Stranded RNA-Seq kit, QCed and pooled evenly at 2nM.
characteristics.characteristic: description
characteristics.text: deletion
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0004014
characteristics.characteristic: genetic modification
characteristics.text: speE
characteristics.characteristic: genotype
characteristics.text: Todd-Hewitt Yeast extract medium
characteristics.characteristic: growth condition
characteristics.text: Streptococcus pneumoniae
characteristics.characteristic: organism
characteristics.text: Streptococcus pneumoniae
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_1313
characteristics.characteristic: organism
characteristics.text: 1
characteristics.characteristic: replicate
characteristics.text: Streptococcus pneumoniae
characteristics.characteristic: scientific_name
characteristics.text: TIGR4
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_170187
characteristics.characteristic: strain
characteristics.text: speE_1
characteristics.characteristic: title