characteristics.text: 2011-12-28T17:00:35Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T15:27:10Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA1031594
characteristics.characteristic: External Id
characteristics.text: NCGR
characteristics.characteristic: INSDC center name
characteristics.text: 2011-12-28T17:00:35Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T15:27:10Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS040157
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-721:SKU-DT-L4
characteristics.characteristic: Submitter Id
characteristics.text: 14 days
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UO_0000033
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: rice
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4530
characteristics.characteristic: common name
characteristics.text: Protocols: Oryza sativa cv. Nipponbare seeds were used in all experiments. Seeds germinated at 28 C in dark for 2 days were transferred to plant growth chamber to grow for 14 days under controlled conditions (12 h light 30 C/12h dark 24 C cycles) to harvest the seedlings. For stress treatments, 14-day-old seedlings were incubated in solutions containing 20% PEG-6000, 200 mM NaCl, for 4 h at 30 C, respectively. For cold treatment seedlings at the same developmental stage were treated at 4 C for 24 h in dark. Total genomic DNA was extracted from leaf tissues using DNase (New England Biolabs), and total RNA was isolated using the TRIzol reagent (Invitrogen). The OligoTex mRNA midi kit (Qiagen) was used to purify poly(A) mRNA from the total RNA samples. Next, mRNAs were fragmented by the RNA fragmentation kit (Ambion). The first cDNA strand was synthesized using random hexamer primers and the second strand cDNA was synthesized where dUTP was used instead of dTTP. Then the dUTP was digested. Small RNAs, ranging from 18-35 bp were enriched by polyacrylamide gel electrophoresis, and ligated to 5' and 3' adapters. The ligation product was reverse-transcript into cDNA, which was then amplified by 15 PCR cycles.
characteristics.characteristic: description
characteristics.text: drought stress
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/EO_0007404
characteristics.characteristic: growth condition
characteristics.text: Oryza sativa
characteristics.characteristic: organism
characteristics.text: Oryza sativa
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4530
characteristics.characteristic: organism
characteristics.text: seedling
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0007131
characteristics.characteristic: organism part
characteristics.text: Oryza sativa
characteristics.characteristic: scientific_name
characteristics.text: SKU-DT-L4
characteristics.characteristic: title