characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2018-04-26T17:02:33Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-09-22T10:52:20Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104313380
characteristics.characteristic: External Id
characteristics.text: Cell Signal Unit, Okinawa Institute of Science and Technology Graduate University
characteristics.characteristic: INSDC center name
characteristics.text: 2018-04-26T17:02:33Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-09-22T10:52:20Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1938358
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-5887:ChIP_Cnot1_LKO_liver_4
characteristics.characteristic: Submitter Id
characteristics.text: 10
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Mice were maintained on a 12 hr light / dark cycle in a temperature-controlled (22ºC) barrier facility with free access to water and a normal diet (NCD, CA-1, CLEA Japan, Inc.). Control (Cnot1fl/fl) and Cnot1 LKO mice placed on tamoxifen-containing food at 6 weeks of age for 2 weeks. Lysate from liver was subjected to ChIP assay with Histone H3 (tri methyl K4) (H3K4me3) antibody (ab8580; abcam) using SimpleChIP Enzyatic Chromatin IP Kit (#9003; Cell Signaling Technology) with modification. Briefly, after micrococcal nuclease treatment, cross-linked chromatin was incubated with anti-H3K4me3 antibody at 4ºC for 24 hr. The immune complex was washed, and DNA was eluted and de-cross-linked. Libraries for DNA sequence were prepared from DNA isolated by ChIP assays with a KAPA Hyper Prep Kit (Illumina), according to the manufacturer’s protocol.
characteristics.characteristic: description
characteristics.text: Alb-Cre-ERT2 / Cnot1 flox/flox
characteristics.characteristic: genotype
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: liver
characteristics.characteristic: organism part
characteristics.text: liver-specific Cnot1 knockout
characteristics.characteristic: phenotype
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: C57BL/6J
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000606
characteristics.characteristic: strain
characteristics.text: ChIP_Cnot1_LKO_liver_4
characteristics.characteristic: title