characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2018-07-22T17:01:55Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-08-15T15:26:59Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104215622
characteristics.characteristic: External Id
characteristics.text: The Rockefeller University Laboratory of Molecular and Cellular Neuroscience
characteristics.characteristic: INSDC center name
characteristics.text: 2018-07-22T17:01:55Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-08-15T15:26:59Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1874640
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-5965:MR36
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: H9
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0003045
characteristics.characteristic: cell line
characteristics.text: dopaminergic neuron
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000700
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: The hESCs H9 (WA-09)-NURR1-GFP reporter line was maintained using E8-essential medium (Fisher Scientific) without feeder on VTA-N (Fisher Scientific) and passaged every 4-5 days by EDTA. Midbrain dopamine (mDA) differentiation from hESC was done with an optimized protocol from previously published by our group. mock (solvent) or 50uM 6OHDA treatment for 16h ChIP was performed and DNA was purified with a magnetic bead-based purification method according to the manufacturer’s protocol (MAGnify Chromatin Immunoprecipitation System-Kit, LifeTechnologies). sheared DNA was used to generate ChIP-sequencing libraries by employing Ovation Ultralow System V2 Kit (NuGEN).
characteristics.characteristic: description
characteristics.text: NURR1::GFP
characteristics.characteristic: genotype
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: embryonic stem cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0002322
characteristics.characteristic: progenitor cell type
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: MR36
characteristics.characteristic: title