characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2017-12-04T17:02:33Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2017-08-29T17:02:29Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104230401
characteristics.characteristic: External Id
characteristics.text: Charite University Hospital Dept of Radiooncology and Radiothearpy
characteristics.characteristic: INSDC center name
characteristics.text: 2017-12-04T17:02:33Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-08-29T17:02:29Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1889419
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-6021:FaDu clone 54
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: FaDu
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002178
characteristics.characteristic: cell line
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Cells were seeded in petri dishes and grown until reaching a confluency of 70-80%. Cells were then harvested by trypsination. Genomic DNA (gDNA) was extracted with the Invisorb Spin Tissue Mini Kit (Stratec, Birkenfeld, Germany). HaloPlex library preparation was performed using approximately 225ng of DNA as recommended by the manufacturer (Agilent, protocol version F1, July 2015). Briefly, the gDNA was digested and the HaloPlex probe library was hybridized in the presence of the indexing primer cassette. The biotinylated DNA-probe hybrids were captured by magnetic purification of the samples with streptavidin-coated magnetic beads. Fragmented targets were amplified via PCR at cycling conditions recommended by the manufacturer. Subsequent to the PCR reaction, the final library was purified using AMPure XP magnetic beads (Beckman Coulter, Krefeld, Germany) and quantified using the library quantification kit Illumina TruSeq (Bio-Rad Laboratories, Munich, Germany) and the QX200 ddPCR System (Bio-Rad Laboratories).
characteristics.characteristic: description
characteristics.text: hypopharyngeal squamous cell carcinoma
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_1001960
characteristics.characteristic: disease
characteristics.text: clone 54
characteristics.characteristic: individual
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: cisplatin-sensitive
characteristics.characteristic: phenotype
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: FaDu clone 54
characteristics.characteristic: title